LOCUS       sequence-346725-        1093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Improved green-red FRET-based PKA activity reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Mo GCH, Posner C, Rodriguez EA, Sun T, Zhang J
  TITLE     A rationally enhanced red fluorescent protein expands the utility of
            FRET biosensors.
  JOURNAL   Nat Commun. 2020 Apr 15;11(1):1848. doi: 10.1038/s41467-020-15687-x.
  PUBMED    32296061
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-15"; volume: "11(1):1848. doi"; pages: " 
            10.1038/s41467-020-15687-x"
COMMENT     Sequence Label: pcDNA3-GR-AKAR3
FEATURES             Location/Qualifiers
     source          1..1093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(55..402)
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     primer_bind     complement(886..907)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctcgctcntg agctcgctgc cgccgtcaac cagcgtcgcg cgcctcaatc cggaaccacc
       61 agcactacca ccagcactac caccagcact accaccagca ctaccaccag cactggtact
      121 accaccagca ctaccaccag cactgccgga accaccagca ctaccaccag cactaccacc
      181 agcactacca ccagcactac caccagcact ggtactacca ccagcactac caccagcact
      241 gccggaacca ccagcactac caccagcact accaccagca ctaccaccag cactaccacc
      301 agcactggta ctaccaccag cactaccacc agcactgccg gaaccaccag cactaccacc
      361 agcactacca ccagcactac caccagcact accaccagca ctggtaccgc gatcaacttt
      421 gttctgctcc aggcactgct taaatttgtc gtttatgaaa atgaccagag ataaaatatc
      481 tgattccacg cctacaccaa cggttatttc atcaccttgg gacagtaact gattgctgtt
      541 cttctcgact ttttgcccat ttaaccaggt cccattagtg gaaatgtcat tcaataaaag
      601 gttaccgtct tctcctagta gtatttggaa atgcttattt gacagtcttg aaatgtttcc
      661 taaatgatag tcacaggctg ggtttctacc aaatgtccaa actttcttta tggatcgttt
      721 ttccttaagc acttgtgaaa tatcagctga caaatctcgg atgggaattt gacccgtggt
      781 acaaatgacc ctgcacacaa tgttttcgcc gatctgttct tgagaaaact tatgcataga
      841 tctcttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      901 gaccatgtga tcgcgcttct cgttggggtc tttgctcngn cggactgggt gctcnngtag
      961 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgtctgctgg tagtggtcgg
     1021 cnanctgcac gctgccgtcn tcgangntnn nggcggnnct tgaagtnngg ccttgatgcc
     1081 ntncttctgc ttg
//
