LOCUS       sequence-346730-       10509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC3D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10509)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 10509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-eGFP-TAX1BP1 CC3D
FEATURES             Location/Qualifiers
     source          1..10509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     327..350
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    404..428
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2610..2634)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2650..3360,3361..3363,3364..3366))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2650..3360,3361..3363,3364..3366))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2692..2713)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3041..3060
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3300..3321
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3363..3372
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3431..3455)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3452..3655)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3481..3501)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3656..3959
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4011..4128
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4421..4465)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4650..4883
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5380..5505
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5552..6185
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6446..6462)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6446..6462)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6459..6481)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6470..6486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6494..6500,6501..6518,6519..6524))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6539..6560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6800..6817)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6971..7559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7051..7070)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7730..8521,8522..8590))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8353..8372
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8591..8695)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8763..8781
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             8900..9133
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    9208..9796
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9314..9325
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9723..9743
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    9822..9943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     9887..9906
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9985..10383)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(join(10407..10509,1..385))
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10455..10474)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 cccctgagcc cgcccccgga cccacccctt cccagcctct gagcccagaa agcgaaggag
       61 caaagctgct attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg
      121 tccatctgca cgagactagt gagacgtgct acttccattt gtcacgtcct gcacgacgcg
      181 agctgcgggg cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg
      241 ccaccaaaga acggagccgg ttggcgccta ccggtggatg tggaatgtgt gcgaggccag
      301 aggccacttg tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc tccagactgc
      361 cttgggaaaa gcgcctcccc tacccggtag aattcgttat cgaaccactt tgtacaagaa
      421 agttgggtag tcaaaattta gaacattctg atcaaaatgg gtctgcacat gcctttcaaa
      481 cacctgctgg tcatagtcag gagggaactg ctcgctgcac atcgggcaca ccttccagtg
      541 actttcaaca tgttcttcaa atttgctctg atcatagtta ggaggaaaca ttaactcaca
      601 gaggggacac ttcttgtgaa catcaaagct ggaatcaaag caaaagcctg tcccatgccc
      661 acgtaaatgt tgacttggag gatcaggagc agtaggcaca ttctcatctt ctttgctatc
      721 ctcagagtcc tctaagccat caggccgact aaagtttcga gcaggctggc tgcagacaac
      781 attgtcttcc agtccccaag aggggactct gacaggtggc ctttgtattt catctgggta
      841 aaaagcacca tctgctccat ctcttgtttc ctgagatgca taaggattac catattgcag
      901 aactggttgt gcatttgaca atgtgagaac ataaccattt tgctctgagc atgttttgaa
      961 acattgagga ctctgggaat tctgaccttc cattttcctt tctgctggtt tgtcagcaat
     1021 ttctttggtc atttcacaga cttgcccctt tgttactatg tcaaaatctg cctctgctgc
     1081 agaaggtgat ggctttacat ctacagtaga ggcagaagtg gctgggtctg tgtttactga
     1141 agcatcattc actttctgct gattccccgt tttctttgtg aagacattat tattattagc
     1201 tgattgatct gaaagtttgt ttatttgttt ttggagcctt tggcattcct taaatttttc
     1261 tttataatgg tctgcagcca tctgaagacg gagtttcaga tcttcaactt ctcttcttag
     1321 ttcgtgttcc agtgtatcag tcttgtcctg atcttttttc atagcattta gtttaagttc
     1381 tgccactgca tcagctaact gctttttcac tttctcgttt tccaagcgtg cagtatgcag
     1441 gtctgccatc gttctgtctc gtacgttgac agcatcactg agttctttag ccagaaagac
     1501 aacttcttgc cgagttgcct gaacctgttc ctctgcttta cgaagttgct cctttaaaaa
     1561 acaagtatct tctttacttg aggttgtaag caggaaagtt ctttgcagat tttccttttc
     1621 agccaaacat aactgcagcc tgccaatctc ttctttgaaa tgagtaatca cgctttcttt
     1681 gttcccatct aaatttttta aagtctggac ctctgacata agcttggtat tttctatttc
     1741 tgtattcttc aaatgtacct tataaagttc cttttcatcc ttctctgtct tcaactgaca
     1801 ttcaagttgt tctctttcat gttgtgcctt cttgagtttg tcctttaaac tgtctaattc
     1861 ggtttctttt tcaattgctt tatgtgttac tgacacaata tcttcctcaa gctgatgggc
     1921 tttggatgta gcatcactga acctcttctt aaactcttca ttttccattt ttaagctttg
     1981 tgttacttca gtaagaccct tttgttctgc ttgcagttgg tcacatcttt ctttctcatg
     2041 gttaagttct ctttccattc tcccaacttg ttctcgaagt tgtgctgttt ctttttccag
     2101 aacggcaatt aactttaaca gttcttcttt ttctttcatg gttttctcaa ttttcaactc
     2161 aagaaggcct gcttttgtgg tcaccactaa catgtcagaa tttccttcat cttccatagt
     2221 aagcagctct tcaactggag aagaagctcg aaactggaaa ggtgtacttg ctccacgaat
     2281 ttcaccctta tgggtaacgt aacagaactg ataaaattct ccatcatcat ttggaaggta
     2341 atatccttgg aatgctagta cacaattgac tgttgatcct tccacataat gttcaggcat
     2401 aggggaccat aaaaacgtgt aataatcacg agcagtactc catccaacct tgaatatacc
     2461 aacccaatct tttggatgtg gatgaatata tggagttaag gtgtaatgac attccaggtg
     2521 tgcattagga aggtaactct tggccacatt ttgaaagatg acatgggcaa agttggaagt
     2581 ctgcaatggg acttcttgaa aggatgtcat gccaactttt ttgtacaaac ttgtttgata
     2641 acctcgagcc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
     2701 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct
     2761 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
     2821 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt
     2881 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
     2941 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
     3001 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
     3061 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
     3121 gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt
     3181 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
     3241 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
     3301 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
     3361 caccatggtg gcagatcggc cgccggtgtc ttctatggag gtcaaaacag cgtggatggc
     3421 gtctccaggc gatctgacgg ttcactaaac gagctctgct tatatagacc tcccaccgta
     3481 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     3541 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     3601 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
     3661 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     3721 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     3781 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     3841 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     3901 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     3961 ggaactccac tagtccaaac tggatctctg ctgtccctgt aataaacccg aaaattttga
     4021 atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact
     4081 attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat
     4141 actgccattt gtgaattcgg cgataccgtc gagatccgtt cactaatcga atggatctgt
     4201 ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg
     4261 ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag
     4321 aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt
     4381 ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta
     4441 attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt
     4501 gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc
     4561 aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa
     4621 tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca
     4681 cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct
     4741 gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt
     4801 gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca
     4861 agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc
     4921 tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata
     4981 attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccggc
     5041 cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc
     5101 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata
     5161 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
     5221 tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct
     5281 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat
     5341 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct
     5401 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc
     5461 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc
     5521 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag
     5581 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     5641 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     5701 caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat
     5761 gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc ctggaaagtc
     5821 cccagcggaa agtcccttgt agcaagctcg atatcagcag ttcttgaagt actccggatg
     5881 cagctctcgg gccacgtgat gaaatgctag gcggctgtca aacctccact ctaacacttc
     5941 tctctccggg tcatccatcc catgcaggct cacagggtgt aacaagctgg tgttctctcc
     6001 tttattggcc tcttctacct tatctggctc aactggtact agcttgtagc accatccaaa
     6061 ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc tgctaatcag ggaagtagcc
     6121 ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt ctttgggagt gaattagccc
     6181 ttccaactac taagtttgta gtacatattt aacaaataca atttctttaa aatgaaaata
     6241 attcagagga atcacaggtt tagagtaaat gaaaccacag gtaattggca gtggtaatag
     6301 ggtatggggt gggaagtttg ggatgatttt ggttagcttg agttatccag ttgatccaga
     6361 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     6421 ctttatttgt gaaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
     6481 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6541 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6601 tgtcgtgcca cggggagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     6661 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     6721 cagccatgag cttgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6781 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6841 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6901 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6961 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7021 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7081 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7141 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7201 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7261 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7321 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7381 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7441 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7501 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7561 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7621 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7681 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7741 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7801 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7861 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7921 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7981 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8041 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8101 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8161 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8221 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8281 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8341 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8401 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8461 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8521 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8581 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8641 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8701 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     8761 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatg atctagccct ttccttaatt
     8821 aacccgggcc tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata
     8881 agatgacatg aactactact gctagagatt ttccacactg actaaaaggg tctgagggat
     8941 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
     9001 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
     9061 tggtctaacc agagagaccc agtacaagca aaaagcagat cttgtcttcg ttgggagtga
     9121 attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac agctgccttg
     9181 taagtcattg gtcttaaagg atctcaggcg gggaggcggc ccaaagggag atccgactcg
     9241 tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag
     9301 gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc
     9361 aggtggcaac acaggcgagc agccaaggaa aggacgatga tttccccgac aacaccacgg
     9421 aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga
     9481 gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg
     9541 tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt
     9601 gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg
     9661 agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc
     9721 cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt
     9781 aatccagagg ttgattagga tctatcgatg catagacatg ataagataca ttgatgagtt
     9841 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     9901 tattgcttta tttgtaacca ttataagctg caataaacaa gtttcgaggt cgagtgtcag
     9961 tcctgctcct cggccacgaa gtgcttagcc ctcccacaca taaccagagg gcagcaattc
    10021 acgaatccca actgccgtcg gctgtccatc actgtccttc actatggctt tgatcccagg
    10081 atgcagatcg agaagcacct gtcggcaccg tccgcagggg ctcaagatgc ccctgttctc
    10141 atttccgatc gcgacgatac aagtcaggtt gccagctgcc gcagcagcag cagtgcccag
    10201 caccacgagt tctgcacaag gtcccccagt aaaatgatat acattgacac cagtgaagat
    10261 gcggccgtcg ctagagagag ctgcgctggc gacgctgtag tcttcagaga tggggatgct
    10321 gttgattgta gccgttgctc tttcaatgag ggtggattct tcttgagaca aaggcttggc
    10381 catggtttaa ttaatgggct gcaggtcgaa aggcccggag atgaggaaga ggagaacagc
    10441 gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga ccttcgggcg
    10501 cccgccccg
//
