LOCUS       sequence-346738-        2112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding a placeholder sequence as a Type 234 part to be 
            used in the Dueber YTK system (MoClo kit).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Camara E, Lenitz I, Nygard Y
  TITLE     A CRISPR activation and interference toolkit for industrial 
            Saccharomyces cerevisiae strain KE6-12.
  JOURNAL   Sci Rep. 2020 Sep 3;10(1):14605. doi: 10.1038/s41598-020-71648-w.
  PUBMED    32884066
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-09-3"; volume: "10(1):14605. doi"; pages: " 
            10.1038/s41598-020-71648-w"
COMMENT     Sequence Label: EC0_6_sgRNA
FEATURES             Location/Qualifiers
     source          1..2112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        305..380
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      complement(477..511)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(582..1241)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1156..1175
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1423..2011
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1912..1931
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcca acgatgtgct tcagtattac attttttgcc ttcaacgcct tgattgttct
       61 atttttgcta ataataaatc tatttcatcg gactaaaagt ccattagttg taagcggatt
      121 tagctcagtt gggagagcgc cagactgaag aaaaacttcg gtcaagtcat ctggaggtcc
      181 tgtgttcgat ccacagaatt cgcagatggc cggcatggtc ccagcctcct cgctggcgcc
      241 ggctgggcaa caccttcggg tggcgaatgg gactcgctat cgtcttctca cgaagactta
      301 ctcagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
      361 aagtggcacc gagtcggtgc tttttttatt ttttgtcact attgttatgt aaaatgccac
      421 ctctgacagt atggaacgca aacttctgtc tagtggatag ctgtgagacc agaccaataa
      481 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
      541 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
      601 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
      661 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
      721 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
      781 gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg
      841 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
      901 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
      961 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     1021 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     1081 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     1141 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     1201 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     1261 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     1321 ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt
     1381 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1441 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1501 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1561 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1621 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1681 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1741 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1801 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1861 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1921 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1981 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2041 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2101 ggataaccgt ag
//
