LOCUS       sequence-346744-        4416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo level 0 Tomato E8 promoter module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Grutzner R, Schubert R, Horn C, Yang C, Vogt T, Marillonnet S
  TITLE     Engineering Betalain Biosynthesis in Tomato for High Level Betanin 
            Production in Fruits.
  JOURNAL   Front Plant Sci. 2021 Jun 9;12:682443. doi: 
            10.3389/fpls.2021.682443. eCollection 2021.
  PUBMED    34177999
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-06-9"; volume: "12:682443. doi"; pages: " 
            10.3389/fpls.2021.682443. eCollection 2021"
COMMENT     Sequence Label: pAGM24951
FEATURES             Location/Qualifiers
     source          1..4416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..58)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        126..230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             452..1243
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1414..2002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1903..1922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2156..2173
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aatgtgagac cacgaagtgg ctcttcagtg gacgaaaggg cctcgtgata cgcctatttt
       61 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
      121 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      181 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgcgctcac
      241 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
      301 tggcttgtta tgactgtttt tttttggggt acagtctatg cctcgggcat ccaagcagca
      361 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
      421 gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg ccgaagtatc
      481 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
      541 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagcg atattgattt
      601 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
      661 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
      721 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
      781 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
      841 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
      901 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
      961 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     1021 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     1081 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     1141 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     1201 ccattacgta aaaggcgaga tcaccaaggt agtcggcaaa taactgtcag accaagttta
     1261 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1321 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1381 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1441 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1501 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1561 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1621 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1681 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1741 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1801 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1861 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1921 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1981 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2041 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2101 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     2161 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     2221 gccgattcat taatcactct gtggtctcag gagttttgtg gaagtgagag agtccaattt
     2281 tgataagaaa agagtcagaa aacgtaatat tttaaaagtc taaatctttc tacaaataag
     2341 agcaaattta tttatttttt aatccaataa atattaatgg aggacaaatt caattcactt
     2401 ggttgtaaaa taaacttaaa ccaataacca aagaactaat aaatcctgaa gtggaattat
     2461 taaggataaa tgtacataga caatgaagaa ataataggtt cgatgaatta ataataatta
     2521 aggatgttac aatcatcatg tgccaagtat atacacaata ttctatggga tttataattt
     2581 cgttacttca cttaactttt gcgtaaataa aacgaattat ctgatatttt ataataaaac
     2641 agttaattaa gaaccatcat ttttaacaac atagatatat tatttctaat agtttaatga
     2701 tacttttaaa tcttttaaat tttatgtttc ttttagaaaa taaaaattca aaaaattaaa
     2761 tatatttaca aaaactacaa tcaaacacaa cttcatatat taaaagcaaa atatattttg
     2821 aaaatttcaa gtgtcctaac aaataagaca agaggaaaat gtacgatgag agacataaag
     2881 agaactaata attgaggagt cctataatat ataataaagt ttattagtaa acttaattat
     2941 taaggactcc taaaatatat gataggagaa aatgaatggt gagagatatt ggaaaactta
     3001 ataattaagg attttaaaat atatggtaaa agataggcaa agtatccatt atcccctttt
     3061 aacttgaagt ctactaggcg catgtgaaag ttgatttttt gtcacgtcat atagctataa
     3121 cgtaaaaaaa gaaagtaaaa tttttaattt tttttaatat atgacatatt ttaaacgaaa
     3181 tataggacaa aatgtaaatg aatagtaaag gaaacaaaga ttaatactta ctttgtaaga
     3241 atttaagata aatttaaaat ttaatagatc aactttacgt ctagaaagac ccatatctag
     3301 aaggaatttc acgaaatcgg cccttattca aaaataactt ttaaataatg aattttaaat
     3361 tttaagaaat aatatccaat gaataaatga catgtagcat tttacctaaa tatttcaact
     3421 attttaatcc aatattaatt tgttttattc ccaacaatag aaagtcttgt gcagacattt
     3481 aatctgactt ttccagtact aaatattaat tttctgaaga ttttcgggtt tagtccacaa
     3541 gttttagtga gaagttttgc tcaaaatttt aggtgagaag gtttgatatt tatcttttgt
     3601 taaattaatt tatctaggtg actattattt atttaagtag aaattcatat cattactttt
     3661 gccaacttgt agtcataata ggagtaggtg tatatgatga aggaataaac aagttcagtg
     3721 aagtgattaa aataaaatat aatttaggtg tacatcaaat aaaaacctta aagtttagaa
     3781 aggcaccgaa taattttgca tagaagatat tagtaaattt ataaaaataa aagaaatgta
     3841 gttgtcaagt tgtgttcttt tttttggata aaaatagcag ttggcttatg tcattctttt
     3901 acaacctcca tgccacttgt ccaattgttg acacttaact aattagtttg attcatgtat
     3961 gaatactaaa taatttttta ggactgactc aaatattttt atattatcat agtaatattt
     4021 atctaatttt taggaccact tattactaaa taataaatta actactacta tattattgtt
     4081 gtgaaacaac aacgttttgg ttgttatgat gaaacgtaca ctatatcagt atgaaaaatt
     4141 caaaacgatt agtataaatt atattgaaaa tttgatattt ttctattctt aatcagacgt
     4201 attgggtttc atattttaaa aagggactaa acttagaaga gaagtttgtt tgaaactact
     4261 tttgtctctt tcttgttccc atttctctct tagatttcaa aaagtgaact actttatctc
     4321 tttctttgtt cacattttat tttattctat tataaatatg gcatcctcat attgagattt
     4381 ttagaaatta ttctaatcat tcacagtgca aaagaa
//
