LOCUS       sequence-34675-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Iwamoto M, Bjoerklund T, Lundberg C, Kirik D, Wandless TJ
  TITLE     A general chemical method to regulate protein stability in the 
            mammalian central nervous system.
  JOURNAL   Chem Biol. 2010 Sep 24. 17(9):981-8.
  PUBMED    20851347
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem Biol. 
            2010 Sep 24. 17(9):981-8."
COMMENT     Sequence Label: pBMN DHFR(DD)-YFP
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..496)
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDYVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     misc_feature    511..885
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(557..573)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
ORIGIN
        1 cgcccttgct cacgcatgct cgccgctcca gaatctcaaa gcaatagctg tgagagttct
       61 gcgcatcagc atcgtggaat tcgctgaata ccgattccca gtcatccggc tcgtaatccg
      121 ggaaatgggt gtcgccttcc acttctgcgt cgatatgcgt cagatacagt ttttgcgctt
      181 ttggcaagaa ctgttcaata acgcgaccgc cgccaatcac catgatttct ggtacgtcac
      241 cacacgccgc gatggcttca tccaccgact tcacccacgt tacgcgatcg tccgtactcg
      301 gttgactgct gaggataata tttttgcgtc ctggcaacgg acgaccgatt gattcccagg
      361 tatggcggcc cataatcacg ggtttattta aggtgttgcg tttaaaccag gcgagatcgg
      421 caggcaggtt ccacggcatg gcgttttcca tgccgataac gtaatctacc gctaacgccg
      481 caatcagact gatcatggtg gcggatccgg cagtctagag gatggtccac ccccggggtc
      541 ggcagccttc acgtgggcgg cgtgtatcca agctgcgatg ccgtctactt tgagggcggt
      601 gggggtggtc agcaggactg tgtaaggtcc tttccagcga ggttctaggt tcttagtctg
      661 gtgtcggcgg acccacactg tgtcgccgac tcggtaaggg tgaggtacca ccggtcggtc
      721 cagttgttct tggtaggctg ccgccagagg tctccagact tcgtgctgga ctaagtagag
      781 agcctgtaag tgagcttgga gagaggggct gttagtaact cttgtcatgt cagggtcagg
      841 gaagtttaca aggggcgggg gtgccccata taagatctca tatggccata tgggggcgcc
      901 t
//
