LOCUS       sequence-346751-       11779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for CLTX CD32a-ITAM CD3z CAR expression in human 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Chang Y, Cai X, Syahirah R, Yao Y, Xu Y, Jin G, Bhute VJ, 
            Torregrosa-Allen S, Elzey BD, Won YY, Deng Q, Lian XL, Wang X, 
            Eniola-Adefeso O, Bao X
  TITLE     CAR-neutrophil mediated delivery of tumor-microenvironment 
            responsive nanodrugs for glioblastoma chemo-immunotherapy.
  JOURNAL   Nat Commun. 2023 Apr 20;14(1):2266. doi: 10.1038/s41467-023-37872-4.
  PUBMED    37080958
REFERENCE   2  (bases 1 to 11779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-20"; volume: "14(1):2266. doi"; pages: " 
            10.1038/s41467-023-37872-4"
COMMENT     Sequence Label: AAVS1-Puro CAG-CLTX CD32a ITAM CD3z CAR
FEATURES             Location/Qualifiers
     source          1..11779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..836
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    843..868
                     /label=SA
                     /note="splice acceptor site"
     CDS             892..945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             955..1554
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(955..974)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1451..1471
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1586..1603)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1592..1816
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        1822..2201
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2520..3536
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3460..3482
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3544..3563
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    5136..5724
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5189..5209)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5607..5618)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5756..6232
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5955..5974)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6342..6361)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6407..6462
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6461..6480)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6823..6839)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6847..6863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6871..6877,6878..6895,6896..6901))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6916..6937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    7011..7847
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7881..7900)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7882..7900)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7907..7924)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7907..7923)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7916..7938)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             8061..8363
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8242..8261
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(8766..8785)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9380..9399
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(9760..10551,10552..10620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10383..10402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10744..11332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11233..11252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11486..11503
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11620..11641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11656..11661,11662..11679,11680..11686)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11694..11710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11718..11734
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11755..11773
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc
      841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagatgacc
      961 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     1021 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     1081 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     1141 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     1201 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     1261 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     1321 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     1381 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     1441 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     1501 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatctaga
     1561 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     1621 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1681 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1741 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1801 gcggtgggct ctatgggtct cgacattgat tattgactag ttattaatag taatcaatta
     1861 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1921 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1981 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     2041 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     2101 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2161 cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt
     2221 tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt
     2281 tttaattatt ttgtgcagcg atgggggcgg gggggggggg gggggggcsc crgggggggg
     2341 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggc grggggsggg
     2401 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
     2461 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
     2521 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
     2581 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     2641 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
     2701 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
     2761 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
     2821 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     2881 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     2941 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     3001 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     3061 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     3121 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     3181 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     3241 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     3301 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     3361 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     3421 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     3481 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     3541 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gtcgacgcca
     3601 ccatgcttct cctggtgaca agccttctgc tctgtgagtt accacaccca gcattcctcc
     3661 tgatcccaat gtgcatgcct tgcttcacca ccgaccacca gatggccaga aagtgcgatg
     3721 actgctgcgg cggcaagggc agaggcaagt gctacggccc ccagtgtctg tgcagagagt
     3781 ccaaatatgg tcccccatgc ccaccatgcc cagcacctga gttcctgggg ggaccatcag
     3841 tcttcctgtt ccccccaaaa cccaaggaca ctctcatgat ctcccggacc cctgaggtca
     3901 cgtgcgtggt ggtggacgtg agccaggaag accccgaggt ccagttcaac tggtacgtgg
     3961 atggcgtgga ggtgcataat gccaagacaa agccgcggga ggagcagttc aacagcacgt
     4021 accgtgtggt cagcgtcctc accgtcctgc accaggactg gctgaacggc aaggagtaca
     4081 agtgcaaggt ctccaacaaa ggcctcccgt cctccatcga gaaaaccatc tccaaagcca
     4141 aagggcagcc ccgagagcca caggtgtaca ccctgccccc atcccaggag gagatgacca
     4201 agaaccaggt cagcctgacc tgcctggtca aaggcttcta ccccagcgac atcgccgtgg
     4261 agtgggagag caatgggcag ccggagaaca actacaagac cacgcctccc gtgctggact
     4321 ccgacggctc cttcttcctc tacagcaggc taaccgtgga caagagcagg tggcaggagg
     4381 ggaatgtctt ctcatgctcc gtgatgcatg aggctctgca caaccactac acacagaaga
     4441 gcctctccct gtccctaggt aaaatcattg tggctgtggt cattgcgact gctgtagcag
     4501 ccattgttgc tgctgtagtg gccttgatct actgcaggaa aaagcggatt tcagccaatt
     4561 ccactgatcc tgtgaaggct gcccaatttg agccacctgg acgtcaaatg attgccatca
     4621 gaaagagaca acttgaagaa accaacaatg actatgaaac agctgacggc ggctacatga
     4681 ctctgaaccc cagggcacct actgacgatg ataaaaacat ctacctgact cttcctccca
     4741 acgaccatgt caacagtaat aacagagtga agttcagcag gagcgcagac gcccccgcgt
     4801 accagcaggg ccagaaccag cagctctata acgagctcaa tctaggacga agagaggagt
     4861 acgatgtttt ggacaagaga cgtggccggg accctgagat ggggggaaag ccgagaagga
     4921 agaaccctca ggaaggcctg tacaatgaac tgcagaaaga taagatggcg gaggcctaca
     4981 gtgagattgg gatgaaaggc gagcgccgga ggggcaaggg gcacgatggc ctttaccagg
     5041 gtctcagtac agccaccaag gacacctacg acgcccttca catgcaggcc ctgccccctc
     5101 gctgaacgcg tgaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
     5161 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5221 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5281 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5341 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5401 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5461 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5521 tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc
     5581 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5641 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     5701 tctccctttg ggccgcctcc ccgcatcgat accgagcgct gctcgagaga tctacgggtg
     5761 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc
     5821 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta
     5881 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg
     5941 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac
     6001 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg
     6061 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt
     6121 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc
     6181 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt
     6241 gtaggtaacc aggcgcgtga attcactcct caggtgcagg ctgcctatca gaaggtggtg
     6301 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
     6361 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
     6421 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
     6481 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
     6541 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
     6601 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
     6661 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
     6721 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
     6781 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
     6841 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6901 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6961 tttccagtcg ggaaacctgt cgtgccagcg gatcgacagt actaagcttt actagggaca
     7021 ggattggtga cagaaaagcc ccatccttag gcctcctcct tcctagtctc ctgatattgg
     7081 gtctaacccc cacctcctgt taggcagatt ccttatctgg tgacacaccc ccatttcctg
     7141 gagccatctc tctccttgcc agaacctcta aggtttgctt acgatggagc cagagaggat
     7201 cctgggaggg agagcttggc agggggtggg agggaagggg gggatgcgtg acctgcccgg
     7261 ttctcagtgg ccaccctgcg ctaccctctc ccagaacctg agctgctctg acgcggctgt
     7321 ctggtgcgtt tcactgatcc tggtgctgca gcttccttac acttcccaag aggagaagca
     7381 gtttggaaaa acaaaatcag aataagttgg tcctgagttc taactttggc tcttcacctt
     7441 tctagtcccc aatttatatt gttcctccgt gcgtcagttt tacctgtgag ataaggccag
     7501 tagccagccc cgtcctggca gggctgtggt gaggaggggg gtgtccgtgt ggaaaactcc
     7561 ctttgtgaga atggtgcgtc ctaggtgttc accaggtcgt ggccgcctct actccctttc
     7621 tctttctcca tccttctttc cttaaagagt ccccagtgct atctgggaca tattcctccg
     7681 cccagagcag ggtcccgctt ccctaaggcc ctgctctggg cttctgggtt tgagtccttg
     7741 gcaagcccag gagaggcgct caggcttccc tgtccccctt cctcgtccac catctcatgc
     7801 ccctggctct cctgcccctt ccctacaggg gttcctggct ctgctctaag ggcgaattcg
     7861 cggccgctaa attcaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
     7921 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     7981 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8041 tgcgcagcct atacgtacgg cagtttaagg tttacaccta taaaagagag agccgttatc
     8101 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc ggggcgacgg atggtgatcc
     8161 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     8221 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     8281 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     8341 acctgatgtt ctggggaata taaatgtcag gcatgagatt atcaaaaagg atcttcacct
     8401 agatcctttt cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca
     8461 gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca
     8521 gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat
     8581 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     8641 tcttgccgcc aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag
     8701 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     8761 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     8821 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     8881 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     8941 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     9001 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     9061 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     9121 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     9181 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     9241 gcatgcccga cggcgaggat ctcgtcgtga cccatgcatg gcgatgcctg cttgccgaat
     9301 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     9361 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     9421 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     9481 ttctatcgcc ttcttgacga gttcttctga attattaacg cttacaattt cctgatgcgg
     9541 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tcaggtggca cttttcgggg
     9601 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg aatactcaac
    10321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgaccaaaat
    10681 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10741 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    10801 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    10861 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    10921 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    10981 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    11041 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    11101 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    11161 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    11221 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    11281 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    11341 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    11401 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    11461 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
    11521 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    11581 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    11641 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    11701 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tcagaattaa
    11761 ccctcactaa agggactag
//
