LOCUS       sequence-346759-        9964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3128
FEATURES             Location/Qualifiers
     source          1..9964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2718..2742)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2750..2791
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2902..3490
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2955..2975)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3373..3384)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3562..3795
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3873..3994
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3910..3929)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3964..3983
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4034..4169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4189..4208)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4190..4208)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4218..4235)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4218..4234)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4227..4249)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4376..4831
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4463..4482)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4673..4694
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4857..4961
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4962..5030,5031..5822)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5180..5199)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5993..6581
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6482..6501
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6735..6752
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6869..6890
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6905..6910,6911..6928,6929..6935)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6943..6959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6948..6970
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6967..6983
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6967..6983
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7002..7022
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7004..7022
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7050..7276
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7277..7457
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7504..7629
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8122..8355
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8540..8584
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8815..9025
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8837..9033
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8884..9019
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9065..9667
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9065..9084)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9561..9581
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9710..9827
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgaacca gtccatccca gtggctccca ccccaccccg
     1261 ccgcgtgcgg ctgaagccct ggctggtggc ccaggtgaac agctgccagt acccagggct
     1321 tcaatgggtc aacggggaaa agaaattatt ctgcatcccc tggaggcatg ccacaaggca
     1381 tggtcccagc caggacggag ataacaccat cttcaaggcc tgggccaagg agacagggaa
     1441 atacaccgaa ggcgtggatg aagccgatcc ggccaagtgg aaggccaacc tgcgctgtgc
     1501 ccttaacaag agccgggact tccgcctcat ctacgacggg ccccgggaca tgccacctca
     1561 gccctacaag atctacgagg tctgctccaa tggccctgct cccacagact cccagccccc
     1621 tgaggattac tcttttggtg caggagagga ggaggaagaa gaggaagagc tgcagaggat
     1681 gttgccaagc ctgagcctca cagaggatgt caagtggccg cccactctgc agccgcccac
     1741 tctgcggccg cctactctgc agccgcccac tctgcagccg cccgtggtgc tgggtccccc
     1801 tgctccagac cccagccccc tggctcctcc ccctggcaac cctgctggct tcagggagct
     1861 tctctctgag gtcctggagc ctgggcccct gcctgccagc ctgccccctg caggcgaaca
     1921 gctcctgcca gacctgctga tcagccccca catgctgcct ctgaccgacc tggagatcaa
     1981 gtttcagtac cgggggcggc caccccgggc cctcaccatc agcaaccccc atggctgccg
     2041 gctcttctac agccagctgg aggccaccca ggagcaggtg gaactcttcg gccccataag
     2101 cctggagcaa gtgcgcttcc ccagccctga ggacatcccc agtgacaagc agcgcttcta
     2161 cacgaaccag ctgctggatg tcctggaccg cgggctcatc ctccagctac agggccagga
     2221 cctttatgcc atccgcctgt gtcagtgcaa ggtgttctgg agcgggcctt gtgcctcagc
     2281 ccatgactca tgccccaacc ccatccagcg ggaggtcaag accaagcttt tcagcctgga
     2341 gcattttctc aatgagctca tcctgttcca aaagggccag accaacaccc caccaccctt
     2401 cgagatcttc ttctgctttg gggaagaatg gcctgaccgc aaaccccgag agaagaagct
     2461 cattactgta caggtggtgc ctgtagcagc tcgactgctg ctggagatgt tctcagggga
     2521 gctatcttgg tcagctgata gtatccggct acagatctca aacccagacc tcaaagaccg
     2581 catggtggag caattcaagg agctccatca catctggcag tcccagcagc ggttgcagcc
     2641 tgtggcccag gcccctcctg gagcaggcct tggtgttggc caggggccct ggcctatgca
     2701 cccagctggc atgcaatacc caactttctt gtacaaagtg gttgatatcg gtaagcctat
     2761 ccctaaccct ctcctcggtc tcgattctac gtagtaatga actagtccgt aacttgaaag
     2821 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacctgca gtacttaaga
     2881 cttaggacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     2941 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3001 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3061 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3121 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3181 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3241 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     3301 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3361 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3421 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3481 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     3541 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     3601 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3661 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3721 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3781 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     3841 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     3901 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3961 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     4021 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4081 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4141 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     4201 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4261 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4321 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     4381 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4441 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4501 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4561 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4621 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4681 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4741 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4801 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     4861 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4921 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4981 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5041 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5101 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5161 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5221 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5281 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5341 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5401 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5461 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5521 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5581 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5641 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5701 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5761 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5821 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     5881 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5941 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6001 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6061 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6121 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6181 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6241 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6301 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6361 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6421 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6481 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6541 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6601 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6661 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6721 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     6781 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     6841 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     6901 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     6961 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     7021 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     7081 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     7141 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     7201 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     7261 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7321 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7381 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7441 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     7501 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     7561 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     7621 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     7681 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     7741 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     7801 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     7861 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     7921 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     7981 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     8041 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     8101 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     8161 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     8221 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     8281 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     8341 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     8401 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     8461 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     8521 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     8581 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     8641 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     8701 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     8761 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     8821 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     8881 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8941 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9001 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct
     9061 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     9121 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     9181 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     9241 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc
     9301 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     9361 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     9421 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     9481 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     9541 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9601 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9661 cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     9721 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     9781 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     9841 gggacagcag agatccactt tggcgccggc tcgaggcctg caggtgcaaa gatggataaa
     9901 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     9961 aatt
//
