LOCUS       sequence-346763-        9958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3131
FEATURES             Location/Qualifiers
     source          1..9958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2712..2736)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2744..2785
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2896..3484
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2949..2969)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3367..3378)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3556..3789
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3867..3988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3904..3923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3958..3977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4028..4163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4183..4202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4184..4202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4212..4229)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4212..4228)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4221..4243)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4370..4825
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4457..4476)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4667..4688
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4851..4955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4956..5024,5025..5816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5174..5193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5987..6575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6476..6495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6729..6746
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6863..6884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6899..6904,6905..6922,6923..6929)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6937..6953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6942..6964
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6961..6977
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6961..6977
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6996..7016
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6998..7016
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7044..7270
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7271..7451
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7498..7623
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8116..8349
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8534..8578
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8809..9019
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8831..9027
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8878..9013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9059..9661
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9059..9078)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9555..9575
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9704..9821
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgtc ttctaattca gatactgggg atttacaaga
     1261 gtctttaaag cacggactta cacctattgt gtctcaattt aaaatggtga attactccta
     1321 tgatgaagat ctggaagagc tttgtcccgt gtgtggagat aaagtgtctg ggtaccatta
     1381 tgggctcctc acctgtgaaa gctgcaaggg attttttaag cgaacagtcc aaaataataa
     1441 aaggtacaca tgtatagaaa accagaactg ccaaattgac aaaacacaga gaaagcgttg
     1501 tccttactgt cgttttcaaa aatgtctaag tgttggaatg aagctagaag ctgtaagggc
     1561 cgaccgaatg cgtggaggaa ggaataagtt tgggccaatg tacaagagag acagggccct
     1621 gaagcaacag aaaaaagccc tcatccgagc caatggactt aagctagaag ccatgtctca
     1681 ggtgatccaa gctatgccct ctgacctgac catttcctct gcaattcaaa acatccactc
     1741 tgcctccaaa ggcctacctc tgaaccatgc tgccttgcct cctacagact atgacagaag
     1801 tccctttgta acatccccca ttagcatgac aatgccccct cacggcagcc tgcaaggtta
     1861 ccaaacatat ggccactttc ctagccgggc catcaagtct gagtacccag acccctatac
     1921 cagctcaccc gagtccataa tgggctattc atatatggat agttaccaga cgagctctcc
     1981 agcaagcatc ccacatctga tactggaact tttgaagtgt gagccagatg agcctcaagt
     2041 ccaggctaaa atcatggcct atttgcagca agagcaggct aaccgaagca agcacgaaaa
     2101 gctgagcacc tttgggctta tgtgcaaaat ggcagatcaa actctcttct ccattgtcga
     2161 gtgggccagg agtagtatct tcttcagaga acttaaggtt gatgaccaaa tgaagctgct
     2221 tcagaactgc tggagtgagc tcttaatcct cgaccacatt taccgacaag tggtacatgg
     2281 aaaggaagga tccatcttcc tggttactgg gcaacaagtg gactattcca taatagcatc
     2341 acaagccgga gccaccctca acaacctcat gagtcatgca caggagttag tggcaaaact
     2401 tcgttctctc cagtttgatc aacgagagtt cgtatgtctg aaattcttgg tgctctttag
     2461 tttagatgtc aaaaaccttg aaaacttcca gctggtagaa ggtgtccagg aacaagtcaa
     2521 tgccgccctg ctggactaca caatgtgtaa ctacccgcag cagacagaga aatttggaca
     2581 gctacttctt cgactacccg aaatccgggc catcagtatg caggctgaag aatacctcta
     2641 ctacaagcac ctgaacgggg atgtgcccta taataacctt ctcattgaaa tgttgcatgc
     2701 caaaagagca ttgccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa
     2761 ccctctcctc ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc
     2821 gatttcttgg ctttatatat cttgtggaaa ggacgatcca gctcacagaa aagcgccccc
     2881 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2941 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3001 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3061 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3121 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3181 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3241 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3301 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3361 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3421 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3481 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     3541 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     3601 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3661 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3721 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3781 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     3841 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     3901 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3961 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     4021 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4081 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4141 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     4201 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4261 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4321 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     4381 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4441 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4501 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4561 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4621 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4681 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4741 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4801 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4861 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4921 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4981 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5041 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5101 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5161 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5221 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5281 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5341 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5401 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5461 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5521 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5581 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5641 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5701 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5761 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5821 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5881 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5941 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6001 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6061 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6121 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6601 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6661 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6721 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6781 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6841 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6901 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6961 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     7021 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     7081 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     7141 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     7201 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     7261 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7321 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7381 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7441 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7501 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7561 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7621 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     7681 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     7741 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     7801 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     7861 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     7921 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     7981 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     8041 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     8101 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     8161 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     8221 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     8281 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     8341 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     8401 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     8461 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     8521 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     8581 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     8641 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     8701 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg
     8761 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     8821 atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8881 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8941 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9001 ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat
     9061 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     9121 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     9181 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     9241 cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag
     9301 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     9361 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     9421 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     9481 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     9541 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     9601 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta
     9661 acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     9721 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     9781 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     9841 gcagagatcc actttggcgc cggctcgagg cctgcaggtg caaagatgga taaagtttta
     9901 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaatt
//
