LOCUS       sequence-346764-       10066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10066)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3132
FEATURES             Location/Qualifiers
     source          1..10066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2820..2844)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2852..2893
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3004..3592
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3057..3077)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3475..3486)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3664..3897
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3975..4096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4012..4031)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4066..4085
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4136..4271
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4291..4310)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4292..4310)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4320..4337)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4320..4336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4329..4351)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4478..4933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4565..4584)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4775..4796
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4959..5063
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5064..5132,5133..5924)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5282..5301)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6095..6683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6584..6603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6837..6854
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6971..6992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7007..7012,7013..7030,7031..7037)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7045..7061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7050..7072
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7069..7085
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7069..7085
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7104..7124
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7106..7124
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7152..7378
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7379..7559
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7606..7731
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8224..8457
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8642..8686
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8917..9127
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8939..9135
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8986..9121
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9167..9769
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9167..9186)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9663..9683
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9812..9929
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagcag gcaccatgga tataaaaaac tcaccatcta gccttaattc
     1261 tccttcctcc tacaactgca gtcaatccat cttacccctg gagcacggct ccatatacat
     1321 accttcctcc tatgtagaca gccaccatga atatccagcc atgacattct atagccctgc
     1381 tgtgatgaat tacagcattc ccagcaatgt cactaacttg gaaggtgggc ctggtcggca
     1441 gaccacaagc ccaaatgtgt tgtggccaac acctgggcac ctttctcctt tagtggtcca
     1501 tcgccagtta tcacatctgt atgcggaacc tcaaaagagt ccctggtgtg aagcaagatc
     1561 gctagaacac accttacctg taaacagaga gacactgaaa aggaaggtta gtgggaaccg
     1621 ttgcgccagc cctgttactg gtccaggttc aaagagggat gctcacttct gcgctgtctg
     1681 cagcgattac gcatcgggat atcactatgg agtctggtcg tgtgaaggat gtaaggcctt
     1741 ttttaaaaga agcattcaag gacataatga ttatatttgt ccagctacaa atcagtgtac
     1801 aatcgataaa aaccggcgca agagctgcca ggcctgccga cttcggaagt gttacgaagt
     1861 gggaatggtg aagtgtggct cccggagaga gagatgtggg taccgccttg tgcggagaca
     1921 gagaagtgcc gacgagcagc tgcactgtgc cggcaaggcc aagagaagtg gcggccacgc
     1981 gccccgagtg cgggagctgc tgctggacgc cctgagcccc gagcagctag tgctcaccct
     2041 cctggaggct gagccgcccc atgtgctgat cagccgcccc agtgcgccct tcaccgaggc
     2101 ctccatgatg atgtccctga ccaagttggc cgacaaggag ttggtacaca tgatcagctg
     2161 ggccaagaag attcccggct ttgtggagct cagcctgttc gaccaagtgc ggctcttgga
     2221 gagctgttgg atggaggtgt taatgatggg gctgatgtgg cgctcaattg accaccccgg
     2281 caagctcatc tttgctccag atcttgttct ggacagggat gaggggaaat gcgtagaagg
     2341 aattctggaa atctttgaca tgctcctggc aactacttca aggtttcgag agttaaaact
     2401 ccaacacaaa gaatatctct gtgtcaaggc catgatcctg ctcaattcca gtatgtaccc
     2461 tctggtcaca gcgacccagg atgctgacag cagccggaag ctggctcact tgctgaacgc
     2521 cgtgaccgat gctttggttt gggtgattgc caagagcggc atctcctccc agcagcaatc
     2581 catgcgcctg gctaacctcc tgatgctcct gtcccacgtc aggcatgcga gtaacaaggg
     2641 catggaacat ctgctcaaca tgaagtgcaa aaatgtggtc ccagtgtatg acctgctgct
     2701 ggagatgctg aatgcccacg tgcttcgcgg gtgcaagtcc tccatcacgg ggtccgagtg
     2761 cagcccggca gaggacagta aaagcaaaga gggctcccag aacccacagt ctcagtgaga
     2821 cccagctttc ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg
     2881 tctcgattct acgtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct
     2941 ttatatatct tgtggaaagg acgatatgat cccccgggcg tatttagcac gcgttaagtc
     3001 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3061 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3121 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3181 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3241 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3301 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3361 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     3421 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3481 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3541 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     3601 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     3661 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     3721 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     3781 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     3841 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     3901 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     3961 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4021 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4081 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     4141 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4201 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4261 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     4321 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4381 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     4441 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     4501 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4561 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4621 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4681 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4741 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4801 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4861 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4921 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4981 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5041 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5101 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5161 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5221 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5281 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5341 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5401 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5461 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5521 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5581 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5641 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5701 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5761 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5821 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5881 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5941 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6001 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6061 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6121 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6181 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6241 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6301 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6361 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6421 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6481 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6541 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6601 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6661 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6721 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6781 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6841 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6901 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     6961 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7021 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7081 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     7141 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     7201 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     7261 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     7321 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     7381 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7441 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7501 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7561 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     7621 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     7681 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     7741 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     7801 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     7861 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     7921 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     7981 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     8041 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     8101 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     8161 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     8221 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     8281 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     8341 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     8401 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     8461 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     8521 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     8581 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     8641 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     8701 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     8761 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     8821 attatcgttt cagacccacc tcccaacccc gaggggaccc atgcattgca tctcaattag
     8881 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8941 cgtctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     9001 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9061 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9121 ctaggctttt gcaaaaagct ttctagaggt accaccggag cttaccatga ccgagtacaa
     9181 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     9241 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     9301 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     9361 ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg
     9421 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     9481 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     9541 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     9601 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     9661 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     9721 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccagttaac gaattctaga
     9781 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     9841 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     9901 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     9961 tttggcgccg gctcgaggcc tgcaggtgca aagatggata aagttttaaa cagagaggaa
    10021 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaatt
//
