LOCUS       sequence-346766-       15889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15889)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 15889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3135
FEATURES             Location/Qualifiers
     source          1..15889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2961..2970
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8643..8667)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8675..8716
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    8827..9415
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8880..8900)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9298..9309)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9487..9720
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9798..9919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9835..9854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9889..9908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9959..10094
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10114..10133)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10115..10133)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10143..10160)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10143..10159)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10152..10174)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10301..10756
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10388..10407)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10598..10619
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10782..10886
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10887..10955,10956..11747)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11105..11124)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11918..12506
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12407..12426
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12660..12677
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12794..12815
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12830..12835,12836..12853,12854..12860)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12868..12884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12873..12895
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12892..12908
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12892..12908
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12927..12947
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12929..12947
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12975..13201
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             13202..13382
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    13429..13554
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    14047..14280
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             14465..14509
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        14740..14950
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        14762..14958
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      14809..14944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             14990..15592
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(14990..15009)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     15486..15506
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    15635..15752
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagcag gcaccatgcc cgccctgcgc cccgctctgc tgtgggcgct
     1261 gctggcgctc tggctgtgct gcgcggcccc cgcgcatgca ttgcagtgtc gagatggcta
     1321 tgaaccctgt gtaaatgaag gaatgtgtgt tacctaccac aatggcacag gatactgcaa
     1381 atgtccagaa ggcttcttgg gggaatattg tcaacatcga gacccctgtg agaagaaccg
     1441 ctgccagaat ggtgggactt gtgtggccca ggccatgctg gggaaagcca cgtgccgatg
     1501 tgcctcaggg tttacaggag aggactgcca gtactcaaca tctcatccat gctttgtgtc
     1561 tcgaccctgc ctgaatggcg gcacatgcca tatgctcagc cgggatacct atgagtgcac
     1621 ctgtcaagtc gggtttacag gtaaggagtg ccaatggacg gatgcctgcc tgtctcatcc
     1681 ctgtgcaaat ggaagtacct gtaccactgt ggccaaccag ttctcctgca aatgcctcac
     1741 aggcttcaca gggcagaaat gtgagactga tgtcaatgag tgtgacattc caggacactg
     1801 ccagcatggt ggcacctgcc tcaacctgcc tggttcctac cagtgccagt gccctcaggg
     1861 cttcacaggc cagtactgtg acagcctgta tgtgccctgt gcaccctcac cttgtgtcaa
     1921 tggaggcacc tgtcggcaga ctggtgactt cacttttgag tgcaactgcc ttccaggttt
     1981 tgaagggagc acctgtgaga ggaatattga tgactgccct aaccacaggt gtcagaatgg
     2041 aggggtttgt gtggatgggg tcaacactta caactgccgc tgtcccccac aatggacagg
     2101 acagttctgc acagaggatg tggatgaatg cctgctgcag cccaatgcct gtcaaaatgg
     2161 gggcacctgt gccaaccgca atggaggcta tggctgtgta tgtgtcaacg gctggagtgg
     2221 agatgactgc agtgagaaca ttgatgattg tgccttcgcc tcctgtactc caggctccac
     2281 ctgcatcgac cgtgtggcct ccttctcttg catgtgccca gaggggaagg caggtctcct
     2341 gtgtcatctg gatgatgcat gcatcagcaa tccttgccac aagggggcac tgtgtgacac
     2401 caacccccta aatgggcaat atatttgcac ctgcccacaa ggctacaaag gggctgactg
     2461 cacagaagat gtggatgaat gtgccatggc caatagcaat ccttgtgagc atgcaggaaa
     2521 atgtgtgaac acggatggcg ccttccactg tgagtgtctg aagggttatg caggacctcg
     2581 ttgtgagatg gacatcaatg agtgccattc agacccctgc cagaatgatg ctacctgtct
     2641 ggataagatt ggaggcttca catgtctgtg catgccaggt ttcaaaggtg tgcattgtga
     2701 attagaaata aatgaatgtc agagcaaccc ttgtgtgaac aatgggcagt gtgtggataa
     2761 agtcaatcgt ttccagtgcc tgtgtcctcc tggtttcact gggccagttt gccagattga
     2821 tattgatgac tgttccagta ctccgtgtct gaatggggca aagtgtatcg atcacccgaa
     2881 tggctatgaa tgccagtgtg ccacaggttt cactggtgtg ttgtgtgagg agaacattga
     2941 caactgtgac cccgatcctt gccaccatgg tcagtgtcag gatggtattg attcctacac
     3001 ctgcatctgc aatcccgggt acatgggcgc catctgcagt gaccagattg atgaatgtta
     3061 cagcagccct tgcctgaacg atggtcgctg cattgacctg gtcaatggct accagtgcaa
     3121 ctgccagcca ggcacgtcag gggttaattg tgaaattaat tttgatgact gtgcaagtaa
     3181 cccttgtatc catggaatct gtatggatgg cattaatcgc tacagttgtg tctgctcacc
     3241 aggattcaca gggcagagat gtaacattga cattgatgag tgtgcctcca atccctgtcg
     3301 caagggtgca acatgtatca acggtgtgaa tggtttccgc tgtatatgcc ccgagggacc
     3361 ccatcacccc agctgctact cacaggtgaa cgaatgcctg agcaatccct gcatccatgg
     3421 aaactgtact ggaggtctca gtggatataa gtgtctctgt gatgcaggct gggttggcat
     3481 caactgtgaa gtggacaaaa atgaatgcct ttcgaatcca tgccagaatg gaggaacttg
     3541 tgacaatctg gtgaatggat acaggtgtac ttgcaagaag ggctttaaag gctataactg
     3601 ccaggtgaat attgatgaat gtgcctcaaa tccatgcctg aaccaaggaa cctgctttga
     3661 tgacataagt ggctacactt gccactgtgt gctgccatac acaggcaaga attgtcagac
     3721 agtattggct ccctgttccc caaacccttg tgagaatgct gctgtttgca aagagtcacc
     3781 aaattttgag agttatactt gcttgtgtgc tcctggctgg caaggtcagc ggtgtaccat
     3841 tgacattgac gagtgtatct ccaagccctg catgaaccat ggtctctgcc ataacaccca
     3901 gggcagctac atgtgtgaat gtccaccagg cttcagtggt atggactgtg aggaggacat
     3961 tgatgactgc cttgccaatc cttgccagaa tggaggttcc tgtatggatg gagtgaatac
     4021 tttctcctgc ctctgccttc cgggtttcac tggggataag tgccagacag acatgaatga
     4081 gtgtctgagt gaaccctgta agaatggagg gacctgctct gactacgtca acagttacac
     4141 ttgcaagtgc caggcaggat ttgatggagt ccattgtgag aacaacatca atgagtgcac
     4201 tgagagctcc tgtttcaatg gtggcacatg tgttgatggg attaactcct tctcttgctt
     4261 gtgccctgtg ggtttcactg gatccttctg cctccatgag atcaatgaat gcagctctca
     4321 tccatgcctg aatgagggaa cgtgtgttga tggcctgggt acctaccgct gcagctgccc
     4381 cctgggctac actgggaaaa actgtcagac cctggtgaat ctctgcagtc ggtctccatg
     4441 taaaaacaaa ggtacttgcg ttcagaaaaa agcagagtcc cagtgcctat gtccatctgg
     4501 atgggctggt gcctattgtg acgtgcccaa tgtctcttgt gacatagcag cctccaggag
     4561 aggtgtgctt gttgaacact tgtgccagca ctcaggtgtc tgcatcaatg ctggcaacac
     4621 gcattactgt cagtgccccc tgggctatac tgggagctac tgtgaggagc aactcgatga
     4681 gtgtgcgtcc aacccctgcc agcacggggc aacatgcagt gacttcattg gtggatacag
     4741 atgcgagtgt gtcccaggct atcagggtgt caactgtgag tatgaagtgg atgagtgcca
     4801 gaatcagccc tgccagaatg gaggcacctg tattgacctt gtgaaccatt tcaagtgctc
     4861 ttgcccacca ggcactcggg gcctactctg tgaagagaac attgatgact gtgcccgggg
     4921 tccccattgc cttaatggtg gtcagtgcat ggataggatt ggaggctaca gttgtcgctg
     4981 cttgcctggc tttgctgggg agcgttgtga gggagacatc aacgagtgcc tctccaaccc
     5041 ctgcagctct gagggcagcc tggactgtat acagctcacc aatgactacc tgtgtgtttg
     5101 ccgtagtgcc tttactggcc ggcactgtga aaccttcgtc gatgtgtgtc cccagatgcc
     5161 ctgcctgaat ggagggactt gtgctgtggc cagtaacatg cctgatggtt tcatttgccg
     5221 ttgtcccccg ggattttccg gggcaaggtg ccagagcagc tgtggacaag tgaaatgtag
     5281 gaagggggag cagtgtgtgc acaccgcctc tggaccccgc tgcttctgcc ccagtccccg
     5341 ggactgcgag tcaggctgtg ccagtagccc ctgccagcac gggggcagct gccaccctca
     5401 gcgccagcct ccttattact cctgccagtg tgccccacca ttctcgggta gccgctgtga
     5461 actctacacg gcacccccca gcacccctcc tgccacctgt ctgagccagt attgtgccga
     5521 caaagctcgg gatggcgtct gtgatgaggc ctgcaacagc catgcctgcc agtgggatgg
     5581 gggtgactgt tctctcacca tggagaaccc ctgggccaac tgctcctccc cacttccctg
     5641 ctgggattat atcaacaacc agtgtgatga gctgtgcaac acggtcgagt gcctgtttga
     5701 caactttgaa tgccagggga acagcaagac atgcaagtat gacaaatact gtgcagacca
     5761 cttcaaagac aaccactgtg accaggggtg caacagtgag gagtgtggtt gggatgggct
     5821 ggactgtgct gctgaccaac ctgagaacct ggcagaaggt accctggtta ttgtggtatt
     5881 gatgccacct gaacaactgc tccaggatgc tcgcagcttc ttgcgggcac tgggtaccct
     5941 gctccacacc aacctgcgca ttaagcggga ctcccagggg gaactcatgg tgtaccccta
     6001 ttatggtgag aagtcagctg ctatgaagaa acagaggatg acacgcagat cccttcctgg
     6061 tgaacaagaa caggaggtgg ctggctctaa agtctttctg gaaattgaca accgccagtg
     6121 tgttcaagac tcagaccact gcttcaagaa cacggatgca gcagcagctc tcctggcctc
     6181 tcacgccata caggggaccc tgtcataccc tcttgtgtct gtcgtcagtg aatccctgac
     6241 tccagaacgc actcagctcc tctatctcct tgctgttgct gttgtcatca ttctgtttat
     6301 tattctgctg ggggtaatca tggcaaaacg aaagcgtaag catggctctc tctggctgcc
     6361 tgaaggtttc actcttcgcc gagatgcaag caatcacaag cgtcgtgagc cagtgggaca
     6421 ggatgctgtg gggctgaaaa atctctcagt gcaagtctca gaagctaacc taattggtac
     6481 tggaacaagt gaacactggg tcgatgatga agggccccag ccaaagaaag taaaggctga
     6541 agatgaggcc ttactctcag aagaagatga ccccattgat cgacggccat ggacacagca
     6601 gcaccttgaa gctgcagaca tccgtaggac accatcgctg gctctcaccc ctcctcaggc
     6661 agagcaggag gtggatgtgt tagatgtgaa tgtccgtggc ccagatggct gcaccccatt
     6721 gatgttggct tctctccgag gaggcagctc agatttgagt gatgaagatg aagatgcaga
     6781 ggactcttct gctaacatca tcacagactt ggtctaccag ggtgccagcc tccaggccca
     6841 gacagaccgg actggtgaga tggccctgca ccttgcagcc cgctactcac gggctgatgc
     6901 tgccaagcgt ctcctggatg caggtgcaga tgccaatgcc caggacaaca tgggccgctg
     6961 tccactccat gctgcagtgg cagctgatgc ccaaggtgtc ttccagattc tgattcgcaa
     7021 ccgagtaact gatctagatg ccaggatgaa tgatggtact acacccctga tcctggctgc
     7081 ccgcctggct gtggagggaa tggtggcaga actgatcaac tgccaagcgg atgtgaatgc
     7141 agtggatgac catggaaaat ctgctcttca ctgggcagct gctgtcaata atgtggaggc
     7201 aactcttttg ttgttgaaaa atggggccaa ccgagacatg caggacaaca aggaagagac
     7261 acctctgttt cttgctgccc gggaggggag ctatgaagca gccaagatcc tgttagacca
     7321 ttttgccaat cgagacatca cagaccatat ggatcgtctt ccccgggatg tggctcggga
     7381 tcgcatgcac catgacattg tgcgccttct ggatgaatac aatgtgaccc caagccctcc
     7441 aggcaccgtg ttgacttctg ctctctcacc tgtcatctgt gggcccaaca gatctttcct
     7501 cagcctgaag cacaccccaa tgggcaagaa gtctagacgg cccagtgcca agagtaccat
     7561 gcctactagc ctccctaacc ttgccaagga ggcaaaggat gccaagggta gtaggaggaa
     7621 gaagtctctg agtgagaagg tccaactgtc tgagagttca gtaactttat cccctgttga
     7681 ttccctagaa tctcctcaca cgtatgtttc cgacaccaca tcctctccaa tgattacatc
     7741 ccctgggatc ttacaggcct cacccaaccc tatgttggcc actgccgccc ctcctgcccc
     7801 agtccatgcc cagcatgcac tatctttttc taaccttcat gaaatgcagc ctttggcaca
     7861 tggggccagc actgtgcttc cctcagtgag ccagttgcta tcccaccacc acattgtgtc
     7921 tccaggcagt ggcagtgctg gaagcttgag taggctccat ccagtcccag tcccagcaga
     7981 ttggatgaac cgcatggagg tgaatgagac ccagtacaat gagatgtttg gtatggtcct
     8041 ggctccagct gagggcaccc atcctggcat agctccccag agcaggccac ctgaagggaa
     8101 gcacataacc acccctcggg agcccttgcc ccccattgtg actttccagc tcatccctaa
     8161 aggcagtatt gcccaaccag cgggggctcc ccagcctcag tccacctgcc ctccagctgt
     8221 tgcgggcccc ctgcccacca tgtaccagat tccagaaatg gcccgtttgc ccagtgtggc
     8281 tttccccact gccatgatgc cccagcagga cgggcaggta gctcagacca ttctcccagc
     8341 ctatcatcct ttcccagcct ctgtgggcaa gtaccccaca cccccttcac agcacagtta
     8401 tgcttcctca aatgctgctg agcgaacacc cagtcacagt ggtcacctcc agggtgagca
     8461 tccctacctg acaccatccc cagagtctcc tgaccagtgg tcaagttcat caccccactc
     8521 tgcttctgac tggtcagatg tgaccaccag ccctacccct gggggtgctg gaggaggtca
     8581 gcggggacct gggacacaca tgtctgagcc accacacaac aacatgcagg tttatgcgtg
     8641 agacccagct ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct
     8701 cggtctcgat tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     8761 gctttatata tcttgtggaa aggacgagtt ctataacagg gcaccaccct gacgcgttaa
     8821 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     8881 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     8941 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     9001 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     9061 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     9121 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     9181 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     9241 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     9301 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     9361 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     9421 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     9481 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     9541 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     9601 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9661 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9721 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     9781 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9841 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9901 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     9961 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    10021 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    10081 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
    10141 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    10201 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    10261 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
    10321 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    10381 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
    10441 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
    10501 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
    10561 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
    10621 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
    10681 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
    10741 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    10801 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    10861 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    10921 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    10981 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    11041 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    11101 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    11161 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    11221 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    11281 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    11341 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    11401 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    11461 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    11521 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    11581 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    11641 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    11701 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    11761 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    11821 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    11881 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    11941 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    12001 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    12061 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    12121 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    12181 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    12241 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    12301 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    12361 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    12421 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    12481 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    12541 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    12601 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    12661 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    12721 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    12781 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    12841 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    12901 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    12961 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
    13021 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
    13081 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
    13141 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
    13201 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    13261 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    13321 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    13381 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
    13441 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
    13501 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
    13561 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
    13621 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
    13681 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
    13741 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
    13801 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
    13861 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
    13921 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
    13981 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
    14041 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
    14101 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
    14161 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
    14221 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
    14281 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
    14341 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
    14401 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
    14461 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
    14521 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
    14581 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
    14641 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
    14701 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    14761 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
    14821 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    14881 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    14941 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
    15001 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
    15061 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
    15121 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
    15181 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
    15241 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
    15301 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
    15361 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
    15421 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
    15481 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
    15541 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
    15601 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    15661 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    15721 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    15781 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
    15841 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
