LOCUS       sequence-346772-       10364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Cre in mammalian cells and enable FACS sorting Cre 
            expressing cells by tdTomato expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10364)
  AUTHORS   Ma H, de Zwaan E, Guo YE, Cejas P, Thiru P, van de Bunt M, Jeppesen 
            JF, Syamala S, Dall'Agnese A, Abraham BJ, Fu D, Garrett-Engele C, 
            Lee TI, Long HW, Griffith LG, Young RA, Jaenisch R
  TITLE     The nuclear receptor THRB facilitates differentiation of human PSCs 
            into more mature hepatocytes.
  JOURNAL   Cell Stem Cell. 2022 Apr 13. pii: S1934-5909(22)00150-3. doi: 
            10.1016/j.stem.2022.03.015.
  PUBMED    35452598
REFERENCE   2  (bases 1 to 10364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2022 Apr 13. pii: S1934-5909(22)00150-3. doi: 
            10.1016/j.stem.2022.03.015."
COMMENT     Sequence Label: Cre-p2a-tdTomato
FEATURES             Location/Qualifiers
     source          1..10364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(13..29)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    37..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(61..67,68..85,86..91))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(244..261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(415..1003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(495..514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1174..1965,1966..2034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1797..1816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2035..2139)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2207..2225
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2263..2285)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2385..2404
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2598..2620
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2612..2629
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2613..2629
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2663..3466
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    3473..3498
                     /label=SA
                     /note="splice acceptor site"
     CDS             3522..3575
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3585..4184
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3585..3604)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4081..4101
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4216..4233)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4222..4446
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        4452..4831
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4833..5110
                     /label=chicken beta-actin promoter
     intron          5111..6127
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6051..6073
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6135..6154
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6191..6211
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6212..7237
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRRNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     CDS             7238..7294
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7307..8737
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     primer_bind     complement(8847..8866)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8912..8967
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8966..8985)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9328..9344)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9352..9368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9376..9382,9383..9400,9401..9406))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9421..9442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    9516..10352
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 gcggccgcca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
       61 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      121 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      181 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      841 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1081 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1141 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1201 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1261 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1321 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1381 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1441 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1501 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1561 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1621 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1681 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1741 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1801 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1861 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1921 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1981 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2041 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2101 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2161 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2221 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2281 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2341 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2401 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2461 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2521 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2581 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattccctgc
     2641 aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc tgggcctgtg
     2701 ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca gatccttccc
     2761 tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc tctgctgtgt
     2821 tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc acgtgatgtc
     2881 ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc cctcacccaa
     2941 ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg cctgtgccat
     3001 ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc gcctcccctt
     3061 cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc gtctccctgg
     3121 ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc tcctgtggat
     3181 tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta cctctctagt
     3241 ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag agctcagcta
     3301 gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt tgctgcctcc
     3361 agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt taatgtggct
     3421 ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc ttctgacctc
     3481 ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga ggaagtcttc
     3541 taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagatgacc gagtacaagc
     3601 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     3661 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     3721 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     3781 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     3841 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     3901 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     3961 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     4021 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     4081 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     4141 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatctaga gggcccgttt
     4201 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4261 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4321 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4381 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4441 ctatgggtct cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     4501 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     4561 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     4621 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     4681 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     4741 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     4801 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
     4861 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
     4921 ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcg ccrggsgggg sggggsgggg
     4981 sgrggggsgg ggsggggcga ggcggagagg kgcggcggca gccaatcaga gcggcgcgct
     5041 ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc
     5101 gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc
     5161 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
     5221 cccttctcct ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg
     5281 gctgcgtgaa agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg
     5341 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg
     5401 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag
     5461 cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt
     5521 gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc
     5581 ccccctgcac ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg
     5641 tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc
     5701 gggcggggcg gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg
     5761 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     5821 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     5881 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     5941 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     6001 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     6061 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     6121 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaccg
     6181 gtccaccatg cccaagaaga agaggaaggt gtccaattta ctgaccgtac accaaaattt
     6241 gcctgcatta ccggtcgatg caacgagtga tgaggttcgc aggaacctga tggacatgtt
     6301 cagggatcgc caggcgtttt ctgagcatac ctggaaaatg cttctgtccg tttgccggtc
     6361 gtgggcggca tggtgcaagt tgaataaccg gaaatggttt cccgcagaac ctgaagatgt
     6421 tcgcgattat cttctatatc ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca
     6481 tttgggccag ctaaacatgc ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa
     6541 tgctgtttca ctggttatgc ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc
     6601 aaaacaggct ctagcgttcg aacgcactga tttcgaccag gttcgttcac tcatggaaaa
     6661 tagcgatcgc tgccaggata tacgtaatct ggcatttctg gggattgctt ataacaccct
     6721 gttacgtata gccgaaattg ccaggatcag ggttaaagat atctcacgta ctgacggtgg
     6781 gagaatgtta atccatattg gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa
     6841 ggcacttagc ctgggggtaa ctaaactggt cgagcgatgg atttccgtct ctggtgtagc
     6901 tgatgatccg aataactacc tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc
     6961 tgccaccagc cagctatcaa ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt
     7021 gatttacggc gctaaggatg actctggtca gagatacctg gcctggtctg gacacagtgc
     7081 ccgtgtcgga gccgcgcgag atatggcccg cgctggagtt tcaataccgg agatcatgca
     7141 agctggtggc tggaccaatg taaatattgt catgaactat atccgtaacc tggatagtga
     7201 aacaggggca atggtgcgcc tgctggaaga tggcgatgcg acgaattttt cgctactaaa
     7261 acaagcgggt gatgtagaag aaaatccggg tccgaccggt ccaaccatgg tgagcaaggg
     7321 cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg
     7381 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     7441 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
     7501 gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa
     7561 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct
     7621 ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat
     7681 gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga
     7741 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc
     7801 cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa
     7861 gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca
     7921 caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt
     7981 cctggggcat ggcaccggca gcaccggcag cggcagctcc ggcaccgcct cctccgagga
     8041 caacaacatg gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
     8101 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     8161 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     8221 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
     8281 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     8341 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
     8401 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
     8461 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     8521 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
     8581 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
     8641 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
     8701 cctgttcctg tacggcatgg acgagctgta caagtaatta attaagatat cgtcgacacg
     8761 cgtgaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     8821 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     8881 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     8941 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     9001 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
     9061 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
     9121 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
     9181 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
     9241 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
     9301 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9361 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9421 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9481 cctgtcgtgc cagcggatcg acagtactaa gctttactag ggacaggatt ggtgacagaa
     9541 aagccccatc cttaggcctc ctccttccta gtctcctgat attgggtcta acccccacct
     9601 cctgttaggc agattcctta tctggtgaca cacccccatt tcctggagcc atctctctcc
     9661 ttgccagaac ctctaaggtt tgcttacgat ggagccagag aggatcctgg gagggagagc
     9721 ttggcagggg gtgggaggga agggggggat gcgtgacctg cccggttctc agtggccacc
     9781 ctgcgctacc ctctcccaga acctgagctg ctctgacgcg gctgtctggt gcgtttcact
     9841 gatcctggtg ctgcagcttc cttacacttc ccaagaggag aagcagtttg gaaaaacaaa
     9901 atcagaataa gttggtcctg agttctaact ttggctcttc acctttctag tccccaattt
     9961 atattgttcc tccgtgcgtc agttttacct gtgagataag gccagtagcc agccccgtcc
    10021 tggcagggct gtggtgagga ggggggtgtc cgtgtggaaa actccctttg tgagaatggt
    10081 gcgtcctagg tgttcaccag gtcgtggccg cctctactcc ctttctcttt ctccatcctt
    10141 ctttccttaa agagtcccca gtgctatctg ggacatattc ctccgcccag agcagggtcc
    10201 cgcttcccta aggccctgct ctgggcttct gggtttgagt ccttggcaag cccaggagag
    10261 gcgctcaggc ttccctgtcc cccttcctcg tccaccatct catgcccctg gctctcctgc
    10321 cccttcccta caggggttcc tggctctgct ctaagggcga attc
//
