LOCUS       sequence-346777-       10040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  positive selection PACE Accessory Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10040)
  AUTHORS   Blum TR, Liu H, Packer MS, Xiong X, Lee PG, Zhang S, Richter M, 
            Minasov G, Satchell KJF, Dong M, Liu DR
  TITLE     Phage-assisted evolution of botulinum neurotoxin proteases with 
            reprogrammed specificity.
  JOURNAL   Science. 2021 Feb 19;371(6531):803-810. doi: 
            10.1126/science.abf5972.
  PUBMED    33602850
REFERENCE   2  (bases 1 to 10040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-02-19- 19"; volume: "371"; 
            issue: "6531"; pages: "803-810"
COMMENT     Sequence Label: AP-VAMP1(X)
FEATURES             Location/Qualifiers
     source          1..10040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..33)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        101..205
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(206..274,275..1066)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGITVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(424..443)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      1117..1163
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             1347..1799
                     /codon_start=1
                     /product="lysozyme from bacteriophage T7"
                     /label=T7 lysozyme
                     /note="inhibitor of T7 RNA polymerase"
                     /translation="MARVQFKQRESTDAIFVHCSATKPSQNVGVREIRQWHKEQGWLDV
                     GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
                     RSLLVTLLAKYEGAVLRAHHEVAPKASPSFDLKRWWEKNELVTSDRG"
     CDS             complement(4653..5633)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="KFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHF
                     SDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGF
                     SDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYT
                     PGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEID
                     HQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITA
                     AKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHTEYT"
     CDS             complement(5697..6776)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(6776..8050)
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     primer_bind     complement(8231..8250)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8232..8250)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      8814..9036
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             9084..10034
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 tatttggcct aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
       61 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      121 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      181 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      241 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      301 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      361 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
      421 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
      481 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
      541 ggatggcata acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
      601 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
      661 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
      721 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
      781 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
      841 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
      901 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
      961 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1021 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaacgta atgctctgcc
     1081 agtgttacaa ccaattaacc aattctgatt tccggtcaaa taaaacgaaa ggctcagtcg
     1141 aaagactggg cctttcgttt tattctagag cacagctaac accacgtcgt ccctatctgc
     1201 tgccctaggt ctatgagtgg ttgctggata actttacggg catgcataag gctcgtaata
     1261 tatattcagg gagaccacaa cggtttccct ctacaaataa ttttgtttaa cttttactag
     1321 agttccttcc tcgaaaggaa aaaaaaatgg ctcgtgtaca gtttaaacaa cgtgaatcta
     1381 ctgacgcaat ctttgttcac tgctcggcta ccaagccaag tcagaatgtt ggtgtccgtg
     1441 agattcgcca gtggcacaaa gagcagggtt ggctcgatgt gggataccac tttatcatca
     1501 agcgagacgg tactgtggag gcaggacgag atgagatggc tgtaggctct cacgctaagg
     1561 gttacaacca caactctatc ggcgtctgcc ttgttggtgg tatcgacgat aaaggtaagt
     1621 tcgacgctaa ctttacgcca gcccaaatgc aatcccttcg ctcactgctt gtcacactgc
     1681 tggctaagta cgaaggcgct gtgcttcgcg cccatcatga ggtggcgccg aaggcttccc
     1741 cttcgttcga ccttaagcgt tggtgggaga agaacgaact ggtcacatct gaccgtggta
     1801 gcggcggtgg tgcgagtggt ggtgcgctgg aacgtgatca gaaactgtct gaactggacg
     1861 accgtgctga cgctctgggc ggtagcgcag gcagtggagc gggcggtaac acgattaaca
     1921 tcgctaagaa cgacttctct gacatcgaac tggctgctat cccgttcaac actctggctg
     1981 accattacgg tgagcgttta gctcgcgaac agttggccct tgagcatgag tcttacgaga
     2041 tgggtgaagc acgcttccgc aagatgtttg agcgtcaact taaagctggt gaggttgcgg
     2101 ataacgctgc cgctaagcct ctcatcacta ccctactccc taagatgatt gcacgcatca
     2161 acgactggtt tgaggaagtg aaagctaagc gcggcaagcg cccgacagcc ttccagttcc
     2221 tgcaagaaat caagccggaa gccgtagcgt acatcaccat taagaccact ctggcttgcc
     2281 taaccagtgc tgacaataca accgttcagg ctgtagcaag cgcaatcggt cgggccattg
     2341 aggacgaggc tcgcttcggt cgtatccgtg accttgaagc taagcacttc aagaaaaacg
     2401 ttgaggaaca actcaacaag cgcgtagggc acgtctacaa gaaagcattt atgcaagttg
     2461 tcgaggctga catgctctct aagggtctac tcggtggcga ggcgtggtct tcgtggcata
     2521 aggaagactc tattcatgta ggagtacgct gcatcgagat gctcattgag tcaaccggaa
     2581 tggttagctt acaccgccaa aatgctggcg tagtaggtca agactctgag actatcgaac
     2641 tcgcacctga atacgctgag gctatcgcaa cccgtgcagg tgcgctggct ggcatctctc
     2701 cgatgttcca accttgcgta gttcctccta agccgtggac tggcattact ggtggtggct
     2761 attgggctaa cggtcgtcgt cctctggcgc tggtgcgtac tcacagtaag aaagcactga
     2821 tgcgctacga agacgtttac atgcctgagg tgtacaaagc gattaacatt gcgcaaaaca
     2881 ccgcatggaa aatcaacaag aaagtcctag cggtcgccaa cgtaatcacc aagtggaagc
     2941 attgtccggt cgaggacatc cctgcgattg agcgtgaaga actcccgatg aaaccggaag
     3001 acatcgacat gaatcctgag gctctcaccg cgtggaaacg tgctgccgct gctgtgtacc
     3061 gcaaggacaa ggctcgcaag tctcgccgta tcagccttga gttcatgctt gagcaagcca
     3121 ataagtttgc taaccataag gccatctggt tcccttacaa catggactgg cgcggtcgtg
     3181 tttacgctgt gtcaatgttc aacccgcaag gtaacgatat gaccaaagga ctgcttacgc
     3241 tggcgaaagg taaaccaatc ggtaaggaag gttactactg gctgaaaatc cacggtgcaa
     3301 actgtgcggg tgtcgataag gttccgttcc ctgagcgcat caagttcatt gaggaaaacc
     3361 acgagaacat catggcttgc gctaagtctc cactggagaa cacttggtgg gctgagcaag
     3421 attctccgtt ctgcttcctt gcgttctgct ttgagtacgc tggggtacag caccacggcc
     3481 tgagctataa ctgctccctt ccgctggcgt ttgacgggtc ttgctctggc atccagcact
     3541 tctccgcgat gctccgagat gaggtaggtg gtcgcgcggt taacttgctt cctagtgaaa
     3601 ccgttcagga catctacggg attgttgcta agaaagtcaa cgagattcta caagcagacg
     3661 caatcaatgg gaccgataac gaagtagtta ccgtgaccga tgagaacact ggtgaaatct
     3721 ctgagaaagt caagctgggc actaaggcac tggctggtca atggctggct tacggtgtta
     3781 ctcgcagtgt gactaagcgt tcagtcatga cgctggctta cgggtccaaa gagttcggct
     3841 tccgtcaaca agtgctggaa gataccattc agccagctat tgattccggc aagggtctga
     3901 tgttcactca gccgaatcag gctgctggat acatggctaa gctgatttgg gaatctgtga
     3961 gcgtgacggt ggtagctgcg gttgaagcaa tgaactggct taagtctgct gctaagctgc
     4021 tggctgctga ggtcaaagat aagaagactg gagagattct tcgcaagcgt tgcgctgtgc
     4081 attgggtaac tcctgatggt ttccctgtgt ggcaggaata caagaagcct attcagacgc
     4141 gcttgaacct gatgttcctc ggtcagttcc gcttacagcc taccattaac accaacaaag
     4201 atagcgagat tgatgcacac aaacaggagt ctggtatcgc tcctaacttt gtacacagcc
     4261 aagacggtag ccaccttcgt aagactgtag tgtgggcaca cgagaagtac ggaatcgaat
     4321 cttttgcact gattcacgac tccttcggta ccattccggc tgacgctgcg aacctgttca
     4381 aagcagtgcg cgaaactatg gttgacacat atgagtcttg tgatgtactg gctgatttct
     4441 acgaccagtt cgctgaccag ttgcacgagt ctcaattgga caaaatgcca gcacttccgg
     4501 ctaaaggtaa cttgaacctc cgtgacatct tagagtcgga cttcgcgttc gcgtaatgga
     4561 gattttcaac atgacgccat gggcattaaa aaatgccctc ttgggttatc aagagggtca
     4621 ttatatttgc tgaggagtgc cgttaattaa gtttaggtat attccgtgtg gtacttctta
     4681 atattatcat caacaatatt gattacattt ttttggctca tcaaatcatt cattggttca
     4741 aaggacagca atacactttt cgcaccacac ttttcaattg ccaacttagc cgcagttata
     4801 cactccgtat aatttccgac agcgttttct gcaattattt cttcaagttt attttcgaaa
     4861 ttttcattag ggtgcatttc aagaacataa tcactaataa atgcacgcgt ctcttgttta
     4921 gctttattac tatcttcgtt atagttaact aatatcatta actgatggtc tatctctgat
     4981 aggtcaacgt catatttatc cgcaacggct ttatatcttt cagcatattc atatctaaca
     5041 tcattagaat catcccactt aaagatgaga ggaatacctt ttttggccgc ccactcaaca
     5101 atatgatgac tggttgctgt tacatatttc cgaggtccgc ctggcgtata agcatgggga
     5161 tttacagata ttttagggaa gctataaaaa tcgttatctg gattacaata gcctgttgtt
     5221 aaagcatcgt taatgatttc ataacactct tcaaatagtt gctgttgata ttcaaccggg
     5281 cgattaaaaa aatgcatttc atcttttttt tcgcaatcac taaaccctaa aataaatctc
     5341 ccttcactta actgatccaa taagcaagct tcctccgcta tgcggacagg atgatgagtt
     5401 gtaatgatgt gatttaatga accaatttta attttctctg ttaaaccgag cagaaaacca
     5461 gaaacagtca gaggagcgcc gacaacacca ttatctgaaa aatgattttc atacactaaa
     5521 atctgttcaa aattcaactt atcaacatac tccgttattt cctgcatgcg aactatactc
     5581 tgttcttgaa cagttgttga attgatgaag ttaaggaaga acaatccaaa tttgctaccg
     5641 ccaccgccgc taccgccacc gccgctaccg ccaccgccgc taccgccacc gccataatat
     5701 aatagcgaac gttgtttttc tttaagaaat ggcatgacat cagactggaa gagcttcatg
     5761 gaagcaataa tttcgtctac tgttccatta gcttcaaatc cacaacaaat atttgatatt
     5821 cctgtagcat caatgtcttt ttgaattatg tcaatacatt cctgcggcgt tcccacggga
     5881 ttgatttcgt aactgtaatc aatacggcga ttagtatctt tatgtccttt taatacaaag
     5941 tcacgccact gccctttatt gaaatcataa cctcttgttt ggtctgaatc atcaaaaata
     6001 gtcgtagcat tcacataaga atcataccaa tgccccagaa atttccggca aatctctttc
     6061 gctttaattg agtcatgatc tacagatgtt atatatgata agcaatggtc gatattatga
     6121 atatcgtgcc catattcttg agccacttca ttataaagct caagttgtgc tttcttttcg
     6181 ttagtattta taatccaact taatatcatc ggtaggccaa attgagcagc ccactcagtc
     6241 gtcgaagctg attcagccac cacataaacc ggtgcgccac ctctgctata cgccgcgggg
     6301 tttactttta ccttatggaa cttgatatgt tcattatcag cttccatata tccctctgtc
     6361 atgccattct ttatcagccc gtaccagcat tccgctaagg cgcgactgtt attcatatct
     6421 gtgccgaata cgcgaaagtc cttgttgtaa agccctcggc aaataccaaa ccgaaatcgt
     6481 ccttttgaca tttgatccaa taaattcaca tcttcaagtt ggcgtactgg atgggctgtg
     6541 ggaagaacaa tagcggcagt tcctacattc aattttttag tcgcgccaag taaatatgca
     6601 gcagcgacat aagggttacc aagcaaacca aactccgtga aatgatgctc cagtaaccat
     6661 acggtatcaa aaccacactc ctcagagatg cgacctaatt taaccaaacg tttcattacc
     6721 tctgtttggg aaaattgggg aggttggtat gtaagcaaaa agtttccaaa tttcattaag
     6781 actccttatt acgcagtatg ttagcaaacg tagaaaatac atacataaag gtggcaacat
     6841 ataaaagaaa cgcaaagaca ccgcggaaca ggttgatctt atcgcagtcg atactgaact
     6901 cgtaaggttt accagcgcca aagacaaaag ggcgacattc aaccgattga gggagggaag
     6961 gtaaatattg acggaaatta ttcattaaag gtgaattatc accgtcaccg acttgagcca
     7021 tttgggaatt agagccagca aaatcaccag tagcaccatt accattagca aggccggaaa
     7081 cgtcaccaat gaaaccatcg atagcagcac cgtaatcagt agcgacagaa tcaagtttgc
     7141 ctttagcgtc agactgtagc gcgttttcat cggcattttc ggtcatagcc cccttattag
     7201 cgtttgccat cttttcataa tcaaaatcac cggaaccaga gccaccaccg gaaccgcctc
     7261 cctcagagcc gccaccctca gaaccgccac cctcagagcc accaccctca gagccgccac
     7321 cagaaccacc accagagccg ccgccagcat tgacaggagg ttgaggcagg tcagacgatt
     7381 ggccttgata ttcacaaacg aatggatcct cattaaagcc agaatggaaa gcgcagtctc
     7441 tgaatttacc gttccagtaa gcgtcataca tggcttttga tgatacagga gtgtactggt
     7501 aataagtttt aacggggtca gtgccttgag taacagtgcc cgtataaaca gttaatgccc
     7561 cctgcctatt tcggaaccta ttattctgaa acatgaaagt attaagaggc tgagactcct
     7621 caagagaagg attaggatta gcggggtttt gctcagtacc aggcggataa gtgccgtcga
     7681 gagggttgat ataagtatag cccggaatag gtgtatcacc gtactcagga ggtttagtac
     7741 cgccaccctc agaaccgcca ccctcagaac cgccaccctc agagccacca ccctcatttt
     7801 cagggatagc aagcccaata ggaacccatg taccgtaaca ctgagtttcg tcaccagtac
     7861 aaactacaac gcctgtagca ttccacagac agccctcata gttagcgtaa cgatctaaag
     7921 ttttgtcgtc tttccagacg ttagtaaatg aattttctgt atggggtttt gctaaacaac
     7981 tttcaacagt ttcagcggag tgagaataga aaggaacaac taaaggaatt gcgaataata
     8041 attttttcat ttttttttcc tttactgcac ctgcaggtct ttcttatgag ttacctcctg
     8101 aacttggtaa acagaacaac aaagctgcct gatagtggcc tttatttttg gcataaataa
     8161 cagaataaac actgcactgt gtattcattc caacgagtga atacacggag caatgtcgct
     8221 ccttctttct ccctatagtg agtcgtatta gtagggtccc taagtctcct cagcaaaacg
     8281 aaaggcccag tctttcgact gagcctttcg ttttatttga ccggatgtcc tcttgacagt
     8341 aagacgggta agcctgttga tgataccgct gccttactgg gtgcattagc cagtctgaat
     8401 gacctgtcac gggataatcc gaagtggtca gactggaaaa tcagagggca ggaactgctg
     8461 aacagcaaaa agtcagatag caccacatag cagacccgcc ataaaacgcc ctgagaagcc
     8521 cgtgacgggc ttttcttgta ttatgggtag tttccttgca tgaatccata aaaggcgcct
     8581 gtagtgccat ttacccccat tcactgccag agccgtgagc gcagcgaact gaatgtcacg
     8641 aaaaagacag cgactcaggt gcctgatggt cggagacaaa aggaatattc agcgatttgc
     8701 ccgagcttgc gagggtgcta cttaagcctt tagggtttta aggtctgttt tgtagaggag
     8761 caaacagcgt ttgcgacatc cttttgtaat actgcggaac tgactaaagt agtgagttat
     8821 acacagggct gggatctatt ctttttatct ttttttattc tttctttatt ctataaatta
     8881 taaccacttg aatataaaca aaaaaaacac acaaaggtct agcggaattt acagagggtc
     8941 tagcagaatt tacaagtttt ccagcaaagg tctagcagaa tttacagata cccacaactc
     9001 aaaggaaaag gactagtaat tatcattgac tagcccatct caattggtat agtgattaaa
     9061 atcacctaga ccaattgaga tgtatgtctg aattagttgt tttcaaagca aatgaactag
     9121 cgattagtcg ctatgactta acggagcatg aaaccaagct aattttatgc tgtgtggcac
     9181 tactcaaccc cacgattgaa aaccctacaa ggaaagaacg gacggtatcg ttcacttata
     9241 accaatacgc tcagatgatg aacatcagta gggaaaatgc ttatggtgta ttagctaaag
     9301 caaccagaga gctgatgacg agaactgtgg aaatcaggaa tcctttggtt aaaggctttg
     9361 agattttcca gtggacaaac tatgccaagt tctcaagcga aaaattagaa ttagttttta
     9421 gtgaagagat attgccttat cttttccagt taaaaaaatt cataaaatat aatctggaac
     9481 atgttaagtc ttttgaaaac aaatactcta tgaggattta tgagtggtta ttaaaagaac
     9541 taacacaaaa gaaaactcac aaggcaaata tagagattag ccttgatgaa tttaagttca
     9601 tgttaatgct tgaaaataac taccatgagt ttaaaaggct taaccaatgg gttttgaaac
     9661 caataagtaa agatttaaac acttacagca atatgaaatt ggtggttgat aagcgaggcc
     9721 gcccgactga tacgttgatt ttccaagttg aactagatag acaaatggat ctcgtaaccg
     9781 aacttgagaa caaccagata aaaatgaatg gtgacaaaat accaacaacc attacatcag
     9841 attcctacct acgtaacgga ctaagaaaaa cactacacga tgctttaact gcaaaaattc
     9901 agctcaccag ttttgaggca aaatttttga gtgacatgca aagtaagcat gatctcaatg
     9961 gttcgttctc atggctcacg caaaaacaac gaaccacact agagaacata ctggctaaat
    10021 acggaaggat ctgaacggcc
//
