LOCUS       sequence-346779-        5655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrative vector for Candida albicans with dominant SAT1 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Dorsaz S, Coste AT, Sanglard D
  TITLE     Red-Shifted Firefly Luciferase Optimized for Candida albicans In 
            vivo Bioluminescence Imaging.
  JOURNAL   Front Microbiol. 2017 Aug 3;8:1478. doi: 10.3389/fmicb.2017.01478. 
            eCollection 2017.
  PUBMED    28824601
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2017-08-3"; volume: "8:1478. doi"; pages: " 
            10.3389/fmicb.2017.01478. eCollection 2017"
COMMENT     Sequence Label: pDS1904
FEATURES             Location/Qualifiers
     source          1..5655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(867..883)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(868..884)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(912..930)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(951..967)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(951..967)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(964..986)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    975..991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(999..1005,1006..1023,1024..1029))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1044..1065
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1182..1199)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1353..1941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1433..1452)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2112..2903,2904..2972))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2735..2754
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2973..3077)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3104..3559
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3191..3210)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3401..3422
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3685..3707
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3699..3716
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3700..3716
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3723..3742
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3723..3741
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agattctaaa acaacatctt tgaaaccaga agaaactttc ttaccttttt cttcggttga
       61 accttcaccg tgcaaagcca ataatgaacc caagtcgaat tttggttgtt tcaacaattt
      121 gacttttctg atgtggacat tttgtaatgg gaaaatggtt tgggtggatt tttcaatttc
      181 acggccaatg acttctggaa tcaatttgga agttaattga gctaaagtac agttggaaac
      241 ttctctttgc ataatttcaa tcatcttctt tctgacttct ctcaatttag aggattgagc
      301 gtaagtagtt ttcttgattt ggtttggttg tcttttggtg aaagcaatgg caaaaactct
      361 caaaacgtaa tcgtcggaag ttttaacagt gacattagct tcaactaatg attgccattt
      421 tctgaccaat gatcttaatt tgtcagaagt gaaatccaaa ccatgaggcc tccatgaggc
      481 ctccatgagg cctccatgag gcctccatgg aggcctcatg gaagttggtc aacaagtttt
      541 taccttggac ttcatcaact ctcaatttga tttttctgta agagtggtct tcggaacctt
      601 gtaagtcggc caaacaaact tcgaaaactc tacctttcaa gccatcagcg gcattcttta
      661 aaccggtaga tctgttgatc aaagttttac caacatttct gttttcaaaa gtggttggag
      721 ctttgatgtc aaaccaatct tttctggtga atgggtcaac gaccttcttt tttaatcctt
      781 tctttccttt ggacaatctc ttgtttttac cgacagccat gttgtacttg agttggatct
      841 acgcgtgaat tcgatatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc
      901 agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg gtcatagctg
      961 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1021 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1081 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1141 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     1201 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1261 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1321 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1381 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1441 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1501 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1561 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1621 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1681 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1741 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1801 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1861 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1921 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1981 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2041 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2101 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2161 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2221 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     2281 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     2341 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     2401 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     2461 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     2521 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     2581 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     2641 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     2701 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     2761 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     2821 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     2881 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2941 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3001 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3061 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
     3121 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3181 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3241 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3301 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3361 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     3421 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     3481 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     3541 atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     3601 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     3661 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     3721 tgtaatacga ctcactatag ggcgaattgg agctccaccg cggtggcggc cgcgtcaaaa
     3781 ctagagaata ataaagaaaa cgatcttttc aaaaagaaaa aaccttttag ttttcctttg
     3841 ttgttgttgt gggtgtgtgc tatttatatt atatagttta ctcataatac cataaaatat
     3901 tcggtttgat taggttattt taataagcta atttgtttct aatcgtgtaa tttatgctgt
     3961 gtatattaag tagtgtgtgc actgcccaaa aatgtttgtt gtttatagtc ggttaaagag
     4021 aaaaaagaaa aaaagatcca tacacacacg ttaattagtt gttcaacgta atacactcat
     4081 attttgttct tatttgcttt cggtcgctgt tctcaccaag atttattgcc aacgaaacaa
     4141 ttttttttta tatattttca gatttttctt tttttccttt cctttccttt tctaattttc
     4201 actcctggtt ttctttcttt cttagaaaca ttatctcgat attaatatta aaaaaatata
     4261 atcattcaaa atggacggtg gtatgtttta gtttagcttc aattctaatt gattgattaa
     4321 tcagttgatt ggtttcaata tgacaaatgg gtagggtggg aaaacttcat tttcaattca
     4381 gatcaaactt ttttgttgtc gacataatat ttctcgtttg ggatgttact gtcacattaa
     4441 taatacacac acatcagctt ataattttga aagtaattta tcagatatgt tgtgacgatc
     4501 agtggaaatg gctaacttca atgtatctgt tcttcccctt tttcaaagtt cacgtttttt
     4561 gattgattga ttgatctgtc ggcagtggtt tcaaaaccat tcggtgagta atcctatcaa
     4621 tcaatgttac gacaaaaggc tcaatattca aaattgcaat gttttatgtt ttcctacgtg
     4681 tacttgtgca aggcaattga ttcaacattg cttttggtgt ttgacgagtt tctagtttgg
     4741 acttgtgttg ttatctgggc tatacagatt tcccggctca ctatgaattt tttttttcga
     4801 cgctcagtgc acacaactat agacaacaca aacacaaaca cagcaagaaa aaaaaaaacg
     4861 aacattgaat tgaaaccaag ccaactgaaa aattccttat ttaaatgact gtcatactaa
     4921 cccattttta tagaagaagt tgctgcttta gttatcgata acggttctca tatgaaaatt
     4981 tcggtgatcc ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt
     5041 gaagtgttcg atgtgcacct atccgaccaa ggctttgaac tatctaccag aagtgtgagc
     5101 ccctaccgga aggattacat ctcggatgat gactctgatg aagactctgc ttgctatggc
     5161 gcattcatcg accaagagct tgtcgggaag attgaactca actcaacatg gaacgatcta
     5221 gcctctatcg aacacattgt tgtgtcgcac acgcaccgag gcaaaggagt cgcgcacagt
     5281 ctcatcgaat ttgcgaaaaa gtgggcacta agcagacagc tccttggcat acgattagag
     5341 acacaaacga acaatgtacc tgcctgcaat ttgtacgcaa aatgtggctt tactctcggc
     5401 ggcattgacc tcttcacgta taaaactaga cctcaagtct cgaacgaaac agcgatgtac
     5461 tggtactggt tctcgggagc acaggatgac gcctaacata tgtgaagtgt gaagggggag
     5521 attttcactt tattagattt gtatatatgt ataataaata aataaataag ttaaataaat
     5581 aattagataa gggtggtaat tattactatt tacaatcaaa ggtggtccgg atcccccaga
     5641 tccttatcct taaac
//
