LOCUS       sequence-346781-        5867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for nucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-173a-16X
FEATURES             Location/Qualifiers
     source          1..5867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3239..3255)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3239..3255)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3252..3274)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3263..3279
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3287..3293,3294..3311,3312..3317))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3332..3353
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3470..3487)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3641..4229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3721..3740)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4400..5191,5192..5260))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5023..5042
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5261..5365)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5433..5451
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5489..5511)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5611..5630
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5824..5846
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5838..5855
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5839..5855
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggtacccggg gatcctgcag tgcttttttt tttcacaatc ccggtgccga ggccgctcaa
       61 ttggtcgtag acagctctag caccgcttaa acgcacgtac ggaatcagat cctgcagtgc
      121 tttttttttt cacaatcccg gtgccgaggc cgctcaattg gtcgtagaca gctctagcac
      181 cgcttaaacg cacgtacgga atcagatcct gcagtgcttt tttttttcac aatcccggtg
      241 ccgaggccgc tcaattggtc gtagacagct ctagcaccgc ttaaacgcac gtacggattc
      301 agatcctgca gtgctttttt ttttcacaat cccggtgccg aggccgctca attggtcgta
      361 gacagctcta gcaccgctta aacgcacgta cggaatcaga tcctgcagtg cttttttttt
      421 tcacaatccc ggtgccgagg ccgctcaatt ggtcgtagac agctctagca ccgcttaaac
      481 gcacgtacgg attcagatcc tgcagtgctt ttttttttca caatcccggt gccgaggccg
      541 ctcaattggt cgtagacagc tctagcaccg cttaaacgca cgtacggaat cagatcctgc
      601 agtgcttttt tttttcacaa tcccggtgcc gaggccgctc aattggtcgt agacagctct
      661 agcaccgctt aaacgcacgt acggattcag atcctgcagt gctttttttt ttcacaatcc
      721 cggtgccgag gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg
      781 gaatcagatc ctgcagtgct tttttttttc acaatcccgg tgccgaggcc gctcaattgg
      841 tcgtagacag ctctagcacc gcttaaacgc acgtacggat tcagatcctg cagtgctttt
      901 ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc tagcaccgct
      961 taaacgcacg tacggaatca gatcctgcag tgcttttttt tttcacaatc ccggtgccga
     1021 ggccgctcaa ttggtcgtag acagctctag caccgcttaa acgcacgtac ggattcagat
     1081 cctgcagtgc tttttttttt cacaatcccg gtgccgaggc cgctcaattg gtcgtagaca
     1141 gctctagcac cgcttaaacg cacgtacgga atcagatcct gcagtgcttt tttttttcac
     1201 aatcccggtg ccgaggccgc tcaattggtc gtagacagct ctagcaccgc ttaaacgcac
     1261 gtacggattc agatcctgca gtgctttttt ttttcacaat cccggtgccg aggccgctca
     1321 attggtcgta gacagctcta gcaccgctta aacgcacgta cggaatcaga tcctgcagtg
     1381 cttttttttt tcacaatccc ggtgccgagg ccgctcaatt ggtcgtagac agctctagca
     1441 ccgcttaaac gcacgtacgg attcagatcc tgcagtgctt ttttttttca caatcccggt
     1501 gccgaggccg ctcaattggt cgtagacagc tctagcaccg cttaaacgca cgtacggaat
     1561 cagatcctgc agtgcttttt tttttcacaa tcccggtgcc gaggccgctc aattggtcgt
     1621 agacagctct agcaccgctt aaacgcacgt acggattcag atcctgcagt gctttttttt
     1681 ttcacaatcc cggtgccgag gccgctcaat tggtcgtaga cagctctagc accgcttaaa
     1741 cgcacgtacg gaatcagatc ctgcagtgct tttttttttc acaatcccgg tgccgaggcc
     1801 gctcaattgg tcgtagacag ctctagcacc gcttaaacgc acgtacggat tcagatcctg
     1861 cagtgctttt ttttttcaca atcccggtgc cgaggccgct caattggtcg tagacagctc
     1921 tagcaccgct taaacgcacg tacggaatca gatcctgcag tgcttttttt tttcacaatc
     1981 ccggtgccga ggccgctcaa ttggtcgtag acagctctag caccgcttaa acgcacgtac
     2041 ggattcagat cctgcagtgc tttttttttt cacaatcccg gtgccgaggc cgctcaattg
     2101 gtcgtagaca gctctagcac cgcttaaacg cacgtacgga atcagatcct gcagtgcttt
     2161 tttttttcac aatcccggtg ccgaggccgc tcaattggtc gtagacagct ctagcaccgc
     2221 ttaaacgcac gtacggattc agatcctgca gtgctttttt ttttcacaat cccggtgccg
     2281 aggccgctca attggtcgta gacagctcta gcaccgctta aacgcacgta cggaatcaga
     2341 tcctgcagtg cttttttttt tcacaatccc ggtgccgagg ccgctcaatt ggtcgtagac
     2401 agctctagca ccgcttaaac gcacgtacgg attcagatcc tgcagtgctt ttttttttca
     2461 caatcccggt gccgaggccg ctcaattggt cgtagacagc tctagcaccg cttaaacgca
     2521 cgtacggaat cagatcctgc agtgcttttt tttttcacaa tcccggtgcc gaggccgctc
     2581 aattggtcgt agacagctct agcaccgctt aaacgcacgt acggattcag atcctgcagt
     2641 gctttttttt ttcacaatcc cggtgccgag gccgctcaat tggtcgtaga cagctctagc
     2701 accgcttaaa cgcacgtacg gaatcagatc ctgcagtgct tttttttttc acaatcccgg
     2761 tgccgaggcc gctcaattgg tcgtagacag ctctagcacc gcttaaacgc acgtacggat
     2821 tcagatcctg cagtgctttt ttttttcaca atcccggtgc cgaggccgct caattggtcg
     2881 tagacagctc tagcaccgct taaacgcacg tacggaatca gatcctgcag tgcttttttt
     2941 tttcacaatc ccggtgccga ggccgctcaa ttggtcgtag acagctctag caccgcttaa
     3001 acgcacgtac ggattcagat cctgcagtgc tttttttttt cacaatcccg gtgccgaggc
     3061 cgctcaattg gtcgtagaca gctctagcac cgcttaaacg cacgtacgga atcagatcct
     3121 gcagtgcttt tttttttcac aatcccggtg ccgaggccgc tcaattggtc gtagacagct
     3181 ctagcaccgc ttaaacgcac gtacggattc agatctgcag aagcttggcg taatcatggt
     3241 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     3301 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     3361 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     3421 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     3481 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3541 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3601 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3661 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3721 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3781 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3841 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3901 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3961 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4021 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4081 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4141 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     4201 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     4261 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     4321 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     4381 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     4441 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4501 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4561 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4621 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4681 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4741 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4801 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4861 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4921 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4981 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     5041 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     5101 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     5161 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     5221 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     5281 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     5341 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     5401 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     5461 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     5521 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     5581 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     5641 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     5701 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     5761 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     5821 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctc
//
