LOCUS       sequence-346791-       11574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP Tagged full length TEM4 protein with R130D mutation. 
            Please see notes below for information regarding the L722F mutation 
            in TEM4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Mitin N, Rossman KL, Der CJ
  TITLE     Identification of a novel actin-binding domain within the Rho 
            guanine nucleotide exchange factor TEM4.
  JOURNAL   PLoS One. 2012;7(7):e41876. doi: 10.1371/journal.pone.0041876. Epub 
            2012 Jul 24.
  PUBMED    22911862
REFERENCE   2  (bases 1 to 11574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7):e41876. doi"; pages: " 
            10.1371/journal.pone.0041876. Epub 2012 Jul 24"
COMMENT     Sequence Label: pEGFP TEM4 R130D
FEATURES             Location/Qualifiers
     source          1..11574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8302..8423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8339..8358)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8393..8412
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8430..8885)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8567..8588)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8779..8798
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8912..9016
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9018..9375
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9018..9209
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9041..9061)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9226..9361
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9410..10204
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9410..10204
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9464..9483)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10074..10093
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10392..10411)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10436..10483
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10812..11400
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11301..11320
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1021 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt ccggccggac tcagatctcg agctcaagct tcgaattcgc cactatggcg
     1321 gacggggcac cccggcccca gctttaccgc agcgtctcgt tcaagctgct ggagcgctgg
     1381 agcggcggcc ccgggctgag ggaggaggac acggacaccc ccggcttgag gcgacgcgcc
     1441 tcgtgccggc cgaccacggc tgcccggggc cagccctctc ggcgcgtgtc caagctggcg
     1501 tctgggcccc tggccgcccc cgcgcagccg cgcccgctcc gcagcctctc gccgtcggtt
     1561 cgccagctct cccggcgctt cgacgcgccg cgtctggacg acggctccgc tgggacccga
     1621 gacggaggcg tcttacccgc ggccgcggaa gaagcggccg agggcccagc gcgaggagcc
     1681 tggcccagcg tcaccgagat ggacaagctc ttcggcggtc ctggctccag gaggcccagc
     1741 gccgactctg aatccccagg aacgcccagc cccgacggtg ccgcgtggga gcctccggct
     1801 cgggagtcgc ggcagccacc gacgccaccc cctcggacat gcttccccct ggcgggtctg
     1861 cgttcggcgc ggcccctgac cgggccggag accgaaggga ggctgcgccg gccgcagcag
     1921 caacaggagc gggcgcagcg tccagcggat ggtttacatt cttggcatat cttctcccaa
     1981 ccgcaggccg gggcccgggc ctcctgctcc tcctcctcca tcgccgcctc ctatcctgtc
     2041 agccgcagtc gtgctgccag ctccagcgag gaggaagagg agggcccgcc gcagctgcct
     2101 ggagcccaga gtccggccta ccacggcggc cactcctcgg gcagtgacga cgaccgagac
     2161 ggtgagggcg gccaccgctg gggagggagg cccgggctca ggcctggaag ctccctattg
     2221 gatcaggact gcaggcctga cagtgatggg ttaaatctaa gcagcatgaa ctcagcaggg
     2281 gtttctggga gccctgagcc cccaacatct ccaagagccc ctagagaaga aggactccgg
     2341 gagtggggta gtggctctcc gccctgcgtc ccaggtcccc aggagggact tcggcctatg
     2401 tctgactctg tgggaggagc tttccgtgtg gccaaggtga gctttccctc gtacctggcc
     2461 agccccgcag gctcccgcgg tagcagccgt tattccagca cggagaccct caaggacgac
     2521 gacctatggt ctagtagggg ttctgggggc tggggcgtgt accgctcccc tagctttgga
     2581 gctggggaag ggctcctgcg gtcccaggct cgaacccgtg ccaaaggacc tggaggcacc
     2641 tctagggcat tgagggatgg aggatttgag cctgaaaaga gtcgacagcg gaagtccctg
     2701 tcaaatccag atatcgcctc agagaccctg acgcttctca gtttcctgcg ctcagacctt
     2761 tcagagctga gggtccgaaa acctggtggg agctccgggg accgtggaag caacccccta
     2821 gatggcagag actcaccatc cgcaggtggc cctgtggggc aacttgaacc catacccatc
     2881 ccagccccag catcacctgg cacgcgcccc acactcaagg acttgacagc cactctgcgg
     2941 agagcaaagt cattcacctg ctctgagaag cccatggccc gccgcctgcc ccgcaccagt
     3001 gctctgaagt ccagctcctc cgagctcctg ctcacaggcc ctggtgccga ggaggatccg
     3061 ctgcccctca tcgtccagga ccaatatgtg caggaggccc gccaggtttt tgagaagatc
     3121 cagcgcatgg gtgcccaaca agatgatgga agcgatgccc cccctggaag ccctgactgg
     3181 gcaggggatg tgacccgagg gcagcggtcc caggaggagc tctcaggccc tgagtccagt
     3241 ctgacagatg aaggcattgg ggcagaccct gagcctcctg ttgcagcatt ttgcggcctg
     3301 ggtaccacag ggatgtggcg acctctttcc tcatcctcgg cccagacgaa ccaccatggc
     3361 cctgggactg aggacagtct gggcgggtgg gccctggtgt cgcctgagac ccctcccaca
     3421 ccaggtgccc tccgccgacg acgcaaagtc ccaccttcag gttctggtgg gagcgaattt
     3481 agcaatgggg aggcagggga ggcctacagg tccctgagtg acccaattcc tcagcgccac
     3541 cgggctgcca cctctgaaga gcctactggg ttctctgtgg acagcaacct cctgggctca
     3601 ctgagcccca agacagggct ccctgccacc tcagccatgg atgagggctt gaccagtggt
     3661 cacagtgact ggtctgtggg cagtgaagag agcaagggat atcaggaggt tattcagagc
     3721 atagttcagg ggcctggcac cctggggcgt gtggtggacg acaggattgc tggcaaagcc
     3781 cccaagaaga aatccctgag tgaccccagc cgccgtgggg agctggctgg gcctggattc
     3841 gagggccctg gaggggagcc catccgagaa gttgagccca tgctgcctcc atccagcagc
     3901 gagcccatcc ttgtagagca gcgggcagag ccagaagaac ctggtgccac caggagccgg
     3961 gcacagtctg aaagggccct acctgaggct ctgcctcccc ctgccactgc ccaccgaaac
     4021 tttcaccttg accccaagct ggctgacatt ctgtccccga ggctaatccg ccgaggctcc
     4081 aagaagcgcc cagctcggag tagtcaccag gagcttcgga gagacgaggg cagtcaggac
     4141 cagactggca gcctgtctcg ggcccggccc tcctccagac acgttcgcca tgccagtgtg
     4201 cccgccacat ttatgcctat tgtggtgcct gagccaccaa cttctgttgg tccccctgtg
     4261 gctgtgccag aacccatagg cttccctacc cgagcccatc ccacgttgca ggcaccatcg
     4321 ctcgaggacg tcaccaagca gtacatgctg aacctgcact ccggtgaggt ccctgcccca
     4381 gtgccagtgg acatgccctg cttgcctctg gctgcaccgc cctctgctga ggccaagccc
     4441 cctgaggcag ctcggcctgc agatgagcct acccctgcca gcaagtgctg cagcaagcca
     4501 caggtggaca tgcggaagca cgtggccatg accctgctgg acacagagca gtcgtatgtg
     4561 gagtcgctgc gcaccctgat gcagggctac atgcagccgc tgaagcagcc agagaactcc
     4621 gtgctctgtg acccttcact ggtggacgag atcttcgacc agatccccga gctcctggag
     4681 caccacgagc aattcctgga gcaggttcgg cactgcatgc agacctggca tgcccagcag
     4741 aaggtgggag ccctgctcgt ccagtcgttc tccaaggatg tcctagtaaa catctattct
     4801 gcctatatcg ataacttcct caatgcaaag gatgctgtgc gtgtggccaa ggaggcgagg
     4861 cctgcctttc tcaagttcct agagcaaagc atgcgtgaga acaaggagaa gcaggcgctg
     4921 tctgacctca tgatcaagcc tgtgcagcgg atcccacgct acgagcttct ggtgaaggac
     4981 ctcctgaagc atacacctga ggaccacccg gaccatccac tcctgctgga ggcgcagcgg
     5041 aacatcaagc aggtggctga gcgcatcaac aagggtgtgc ggagtgccga ggaggcggag
     5101 cgccatgccc gtgtgctgca ggagatagag gctcacatcg agggcatgga ggatctccag
     5161 gcccctctgc ggcggttcct gagacaggag atggtcattg aagtgaaggc gatcggtggc
     5221 aagaaggacc ggtctctctt cctgttcacg gacctcatcg tctgcaccac tctgaagcga
     5281 aagtcaggct ccctgcggcg cagctccatg agcctgtaca cggcagccag tgtcattgac
     5341 acagccagca agtacaagat gctgtggaag ctgccgctgg aagacgcaga catcatcaaa
     5401 ggggcatccc aagccaccaa tcgggagaac atccagaagg ccatcagccg ccttgatgag
     5461 gacctcacca ccctgggcca aatgagcaag ctctctgaga gccttggttt cccccaccag
     5521 agcctggacg atgcactgcg ggacctctca gctgccatgc accgggacct gtcggagaag
     5581 caggcgctgt gctacgcgct ttccttcccg ccaaccaagc tggagctgtg cgccactcgg
     5641 cccgagggca ccgactccta catttttgag ttccctcacc ctgacgcccg ccttggtttt
     5701 gaacaggcct tcgatgaggc caagaggaag ctggcatcca gcaaaagctg tctagaccct
     5761 gagttcctga aggccatccc catcatgaaa acccgcagtg gcatgcagtt ctcctgtgcg
     5821 gctcccaccc tgaacagctg cccggagccc tcgcctgagg tatgggtctg caacagcgac
     5881 ggctacgtgg gccaggtgtg cctgctgagc ctgcgcgccg agccggacgt ggaggcctgc
     5941 atcgccgtct gttccgcccg catcctctgc atcggggcgg tgcccgggct gcagcctcgc
     6001 tgccaccggg agcctcctcc gtcgctgagg agtcctccag agacggcacc ggagcccgcc
     6061 gggccggagc tggacgtcga ggccgctgca gacgaggaag ccgcgacgct cgcggagccg
     6121 gggccgcagc cctgccttca catctccatt gcaggctcgg gcttggagat gacgccgggc
     6181 ctcggcgagg gtgacccccg cccagagctg gtgccctttg acagtgactc tgacgatgag
     6241 tcttcgccca gcccctcggg gacgctgcag agccaggcca gccggtccac catctcctcc
     6301 agctttggca atgaggagac cccgagttcc aaggaggcca cggcagagac caccagctca
     6361 gaggaggagc aggagccagg cttcctgcca ctgtctggct cctttgggcc tggtggtccc
     6421 tgcggcacca gcccaatgga tgggagagcc cttcgccgct ccagccacgg ctccttcacc
     6481 cggggcagcc ttgaggacct gctgagtgtc gaccctgagg cctaccagag ctccgtgtgg
     6541 ctgggcactg aggatggctg tgtccacgtg taccagtcct ccgacagcat ccgtgaccgc
     6601 aggaacagca tgaagctcca gcatgcggcc tctgtgacct gcatcttgta tctgaataac
     6661 caggtgtttg tgtctctggc caatggagag cttgtggtct accaaaggga agcaggccat
     6721 ttctgggacc cccagaactt caaatcagtg accttgggca cccaggggag ccccatcacc
     6781 aagatggtat ctgtgggtgg gcggctgtgg tgtggctgcc agaaccgagt ccttgtcctg
     6841 agccctgaca cgctgcagct ggagcacatg ttttacgtgg gtcaggattc aagccgctgc
     6901 gtggcttgca tggtggactc cagcctgggt gtgtgggtga cattgaaagg tagtgcccac
     6961 gtgtgtctct accatccaga cacctttgag cagctggcag aagtagacgt cactcctccc
     7021 gtgcacagga tgctggcagg ctcggatgcc atcatccggc agcacaaggc tgcctgtctg
     7081 cgaatcacag cgctgctggt gtgtgaggag ctgctgtggg tgggcaccag tgctggtgtc
     7141 gtcctcacca tgcccacttc gcccggtact gtcagctgcc cacgggcacc actcagtccc
     7201 acaggcctcg gccagggaca caccggccac gtccgcttct tggctgcagt ccagctgcca
     7261 gatggcttca acctgctctg cccaacccca ccacctcccc cagacacagg ccccgagaag
     7321 ctgccatcac tggagcaccg ggactcccct tggcaccgag gccccgcccc tgccaggcct
     7381 aaaatgctgg ttatcagtgg aggtgatggc tatgaggact tccgactcag cagtgggggc
     7441 ggcagcagca gtgagactgt gggtcgagac gacagcacaa accacctcct cctgtggagg
     7501 gtgtgaccct gtctgccgtg gcccaggact cgcccgccca cctgccttca gcctgcttgc
     7561 ctctccctag cccacacgca gactttgacc aggagtatcc agccaggggc acacatgtgc
     7621 ctgcgtgggc tctgccttgt cttcgcggaa gcattcctga tggaacaccc actggccagc
     7681 caggccatgg cttctcccga ccctctggct gccccggtgc ttccagtcat gatcgggtgg
     7741 gggacatgtg ggctgaccag gacctctgac cctggagctt ctaccaaaga cacagctggg
     7801 tctggacccc acggggctgg ggagggccat gtgcaatatt tggagggttt tctggagggc
     7861 agcaggaagg ctggggaatt ccccatgtac agtatttatg tttcttttta gatgtgtacc
     7921 ttcccaagca cttatttatg cagtgacctg gtcacctggg gtgggggtga tttgaggaaa
     7981 tgacatgagg aaaagaaacc tattcctgcc ctggggacca ccctgggact ctaaccaagc
     8041 cttcctggag ggacccatgc gcccctgagc cccattccat tcatacagac acacacgtac
     8101 gcacactgca tgtccaaggc cctaaacatt gcccgttgac ataaactttc cagggcccca
     8161 gcctgatggg gctgccctca gtcctctaga taactgatca taatcagcca taccacattt
     8221 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     8281 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     8341 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8401 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt
     8461 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     8521 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     8581 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     8641 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     8701 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     8761 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     8821 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     8881 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     8941 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     9001 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     9061 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     9121 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9181 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     9241 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     9301 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9361 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     9421 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     9481 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     9541 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     9601 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     9661 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     9721 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     9781 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     9841 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     9901 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     9961 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
    10021 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
    10081 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
    10141 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
    10201 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
    10261 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
    10321 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
    10381 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
    10441 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
    10501 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
    10561 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
    10621 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
    10681 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    10741 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    10801 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10861 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10921 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    10981 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    11041 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    11101 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    11161 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    11221 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    11281 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    11341 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    11401 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    11461 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
    11521 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccg
//
