LOCUS       sequence-346842-        9901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3138
FEATURES             Location/Qualifiers
     source          1..9901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2655..2679)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2687..2728
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2839..3427
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2892..2912)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3310..3321)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3499..3732
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3810..3931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3847..3866)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3901..3920
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3971..4106
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4126..4145)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4127..4145)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4155..4172)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4155..4171)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4164..4186)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4313..4768
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4400..4419)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4610..4631
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4794..4898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4899..4967,4968..5759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5117..5136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5930..6518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6419..6438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6672..6689
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6806..6827
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6842..6847,6848..6865,6866..6872)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6880..6896
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6885..6907
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6904..6920
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6904..6920
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6939..6959
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6941..6959
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6987..7213
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7214..7394
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7441..7566
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8059..8292
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8477..8521
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8752..8962
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8774..8970
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8821..8956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9002..9604
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9002..9021)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9498..9518
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9647..9764
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgaccat ggttgacaca gagatgccat tctggcccac
     1261 caactttggg atcagctccg tggatctctc cgtaatggaa gaccactccc actcctttga
     1321 tatcaagccc ttcactactg ttgacttctc cagcatttct actccacatt acgaagacat
     1381 tccattcaca agaacagatc cagtggttgc agattacaag tatgacctga aacttcaaga
     1441 gtaccaaagt gcaatcaaag tggagcctgc atctccacct tattattctg agaagactca
     1501 gctctacaat aagcctcatg aagagccttc caactccctc atggcaattg aatgtcgtgt
     1561 ctgtggagat aaagcttctg gatttcacta tggagttcat gcttgtgaag gatgcaaggg
     1621 tttcttccgg agaacaatca gattgaagct tatctatgac agatgtgatc ttaactgtcg
     1681 gatccacaaa aaaagtagaa ataaatgtca gtactgtcgg tttcagaaat gccttgcagt
     1741 ggggatgtct cataatgcca tcaggtttgg gcggatgcca caggccgaga aggagaagct
     1801 gttggcggag atctccagtg atatcgacca gctgaatcca gagtccgctg acctccgggc
     1861 cctggcaaaa catttgtatg actcatacat aaagtccttc ccgctgacca aagcaaaggc
     1921 gagggcgatc ttgacaggaa agacaacaga caaatcacca ttcgttatct atgacatgaa
     1981 ttccttaatg atgggagaag ataaaatcaa gttcaaacac atcacccccc tgcaggagca
     2041 gagcaaagag gtggccatcc gcatctttca gggctgccag tttcgctccg tggaggctgt
     2101 gcaggagatc acagagtatg ccaaaagcat tcctggtttt gtaaatcttg acttgaacga
     2161 ccaagtaact ctcctcaaat atggagtcca cgagatcatt tacacaatgc tggcctcctt
     2221 gatgaataaa gatggggttc tcatatccga gggccaaggc ttcatgacaa gggagtttct
     2281 aaagagcctg cgaaagcctt ttggtgactt tatggagccc aagtttgagt ttgctgtgaa
     2341 gttcaatgca ctggaattag atgacagcga cttggcaata tttattgctg tcattattct
     2401 cagtggagac cgcccaggtt tgctgaatgt gaagcccatt gaagacattc aagacaacct
     2461 gctacaagcc ctggagctcc agctgaagct gaaccaccct gagtcctcac agctgtttgc
     2521 caagctgctc cagaaaatga cagacctcag acagattgtc acggaacacg tgcagctact
     2581 gcaggtgatc aagaagacgg agacagacat gagtcttcac ccgctcctgc aggagatcta
     2641 caaggacttg tactacccaa ctttcttgta caaagtggtt gatatcggta agcctatccc
     2701 taaccctctc ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat
     2761 ttcgatttct tggctttata tatcttgtgg aaaggacgag tataacgccg gagatccacg
     2821 acaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2881 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2941 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3001 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3061 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3121 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3181 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     3241 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3301 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3361 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3421 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3481 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     3541 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3601 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3661 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3721 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     3781 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     3841 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3901 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     3961 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4021 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4081 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     4141 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4201 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4261 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     4321 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4381 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4441 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4501 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4561 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4621 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4681 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4741 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     4801 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4861 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4921 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4981 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5041 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5101 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5161 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5221 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5281 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5341 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5401 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5461 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5521 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5581 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5641 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5701 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5761 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5821 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5881 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5941 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6001 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6061 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6121 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6181 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6241 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6301 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6361 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6421 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6481 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6541 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6601 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6661 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6721 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6781 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6841 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6901 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     6961 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     7021 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     7081 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     7141 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     7201 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7261 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7321 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7381 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     7441 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     7501 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7561 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7621 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     7681 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     7741 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7801 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7861 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     7921 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7981 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8041 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     8101 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     8161 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     8221 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     8281 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8341 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     8401 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8461 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8521 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8581 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     8641 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
     8701 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8761 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
     8821 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8881 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8941 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
     9001 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     9061 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     9121 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     9181 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
     9241 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     9301 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     9361 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     9421 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     9481 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     9541 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
     9601 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     9661 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     9721 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     9781 acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt
     9841 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
     9901 t
//
