LOCUS       sequence-34686-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH
  TITLE     Drosophila Brainbow: a recombinase-based fluorescence labeling 
            technique to subdivide neural expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):253-9. doi: 10.1038/nmeth.1566. Epub 2011
            Feb 6.
  PUBMED    21297621
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1566"; journalName: "Nat Methods"; date: "2011-03"; 
            volume: "8"; issue: "3"; pages: "253-9"
COMMENT     Sequence Label: dBrainbow
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     141..162
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    204..237
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    259..292
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    314..347
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          487..552
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             682..702
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
       61 tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc
      121 agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt attgaataca
      181 agaagagaac tctgaataga tctataactt cgtataaagt atcctatacg aagttatgag
      241 gcagcgcggc tatcgtggat aacttcgtat aatgtgtact atacgaagtt atctggccac
      301 gacgggcgtt cctataactt cgtataatgt atgctatacg aagttatgcc ctgaatgcgg
      361 ccgccagtgt gatggatatc tgcagaattc gcccttcggc cgccaaaatg gatctttgtg
      421 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
      481 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
      541 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
      601 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
      661 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
      721 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
      781 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
      841 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
      901 tttacttgct ttaaaaaacc tc
//
