LOCUS       sequence-34690-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Inoki K, Zhu T, Guan KL
  TITLE     TSC2 mediates cellular energy response to control cell growth and 
            survival.
  JOURNAL   Cell. 2003 Nov 26. 115(5):577-90.
  PUBMED    14651849
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2003 
            Nov 26. 115(5):577-90."
COMMENT     Sequence Label: TSC2-S1345A
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..183
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 tagcgagctc tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttgccatg gcctacccat acgatgttcc tgactatgcg
      121 ggctatccct atgacgtccc ggactatgca ggatcctatc catatgacgt tccagattac
      181 gctgctcagt gcggccgcat ggccaaacca actagcaaag attcaggttt gaaggagaag
      241 ttcaagatac tattaggact gggaacgtca aggccaaatc ctaggtgtgc agaaggcaaa
      301 cagacggagt ttatcatcac agcggaaatc ttgagagaac tgagtggtga atgcggcctc
      361 aacaatcgaa tccggatgat agggcagatc tgtgatgtgg caaaaactaa gaaacttgaa
      421 gagcatgcag tggaggcact ttggaaggct gtctcagact tgctacagcc agagcggcca
      481 ccggaggccc gacatgcagt tctggccttg ttgaaggcca ttgtgcaggg acagggtgat
      541 cgtttggggg ttctccgagc cctcttcttc aaagtgatca aggactatcc ttccaacgaa
      601 gacctccatg aaaggctaga ggtttttaag gccctcacag acaatggaag gcatattacc
      661 tacttggaag aagaactggc agagtttgtc ctgcagtgga tggatgttgg cttgtcctca
      721 gaattccttc tggtactcgt gaacctggtc aagttcaaca gctgctacct tgacgaatac
      781 attgcgccaa tggttcacat gatttgtctg ttatgtatcc ggacagtgtc ctctgtggac
      841 atcgaggtgt ccttgcaagt gctggatgct gtggtctgct acaactgcct gcctgctgaa
      901 aac
//
