LOCUS       sequence-346958-        4314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible gene expression, pCDF origin, LacI repressor, PLlacO-1 
            promoter, mRFP, Streptomycin Resistance Marker. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4314)
  TITLE     Reisch Lab Duet Plasmids
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDLlR6
FEATURES             Location/Qualifiers
     source          1..4314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(28..46)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      42..89
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(260..1051)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1052..1143)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1191..1929)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     2139..2155
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2206..3288)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     3250..3269
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3289..3366)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      complement(3430..3473)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3476..3497
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    3500..3516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3523..3539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3591..3602
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             3609..4286
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 gctttccagt cgggaaacct gctgccaccg ctgagcaata actagcataa ccccttgggg
       61 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
      121 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
      181 aacgaccctg ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg gcccctcggc
      241 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      301 ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      361 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      421 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      481 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      541 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      601 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      661 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      721 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      781 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      841 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      901 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      961 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
     1021 tcgatacttc ggcgatcacc gcttccctca tactcttcct ttttcaatat tattgaagca
     1081 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     1141 aaatagctag ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga
     1201 cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa
     1261 cagcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat
     1321 aaacagacgc ttttccggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag
     1381 tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggcgggt cgctccctct
     1441 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt tctcccttac
     1501 gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt gtaggtcgtt
     1561 cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg cgccttatcc
     1621 ggtaactgtt cacttgagtc caacccggaa aagcacggta aaacgccact ggcagcagcc
     1681 attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg ctcttgaagt
     1741 gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc tgcgaaagcc
     1801 agttaccacg gttaagcagt tccccaactg ncttaacctt cgatcaaacc acctccccag
     1861 gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat
     1921 cctttgatct tttctactga accgctctag atttcagtgc aatttatctc ttcaaatgta
     1981 gcacctgaag tcagccccat acgatataag ttgtaattct catgttagtc atgccccgcg
     2041 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg
     2101 cctaatgagt gagctaactt acattaattg cgttgcgcgt aaaacgacgg ccagtgagat
     2161 cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc
     2221 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2281 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
     2341 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     2401 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
     2461 ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat
     2521 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
     2581 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
     2641 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
     2701 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     2761 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     2821 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
     2881 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
     2941 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
     3001 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
     3061 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
     3121 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     3181 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
     3241 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     3301 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     3361 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaaa tggcgtatca
     3421 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt
     3481 cgcggccgct tctagagaat tgtgagcgga taacaattga cattgtgagc ggataacaag
     3541 atactgagca caccctgtag gaactgggaa gagcgcaaca catactagag aaagaggaga
     3601 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
     3661 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
     3721 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
     3781 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
     3841 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
     3901 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
     3961 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
     4021 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
     4081 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
     4141 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
     4201 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
     4261 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgc
//
