LOCUS       sequence-346959-        9856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde 
            transport of recycling endosomes. Compatible with Flp-in TREX 
            system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Nijenhuis W, van Grinsven MMP, Kapitein LC
  TITLE     An optimized toolbox for the optogenetic control of intracellular 
            transport.
  JOURNAL   J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 
            10.1083/jcb.201907149.
  PUBMED    32328628
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-04-6"; volume: "219(4). pii: 133834. doi"; 
            pages: " 10.1083/jcb.201907149"
COMMENT     Sequence Label: 
            pCDNA5-FRT-TO-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCher
            ry-RAB11
FEATURES             Location/Qualifiers
     source          1..9856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(167..186)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     514..535
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(707..913,914..916,917..1036)
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein and with the mutation R74Q to reduce the 
                     affinity for iLID"
                     /label=SspB micro
                     /note="dimerizes with iLID at moderate affinity to create 
                     iLID micro (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     CDS             1049..1105
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1112..1549
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     CDS             1589..2293
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1731..1749)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(3072..3089)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3078..3302
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3435..3454)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    3586..3633
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             3641..4663
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             3641..4663
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    4793..4914
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4830..4849)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4884..4903
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4963..4979)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4963..4979)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4976..4998)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4987..5003
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5011..5017,5018..5035,5036..5041))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5056..5077
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5194..5211)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5365..5953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5445..5464)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6124..6915,6916..6984))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6747..6766
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6985..7089)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7164..7183)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7355..7734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7735..7938
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7889..7909
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    7940..7958
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7961..7979
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     7989..8013
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      8115..8124
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      9097..9106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9720..9722,9723..9856,1..577)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(9720..9722,9723..9856,1..577)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9762..9783)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gatctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctcggcta
       61 cggcctgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      121 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      181 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      241 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
      301 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg caaacttcaa
      361 gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac
      421 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa
      481 actgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
      541 cgccgggatc actctcggca tggacgagct gtacaaggct agcggaggcg gtggctcagg
      601 cggaggaggg tcaggagggg gaggcagtgg cgggggcggc tctggaggag ggggttctgg
      661 tggtggtagc tacgtagatc tgagcggcct gaccctgcag gaattcagct ccccgaaacg
      721 ccctaagctg ctgcgtgaat attacgattg gctggttgat aacagcttta ccccatatct
      781 ggtggtggat gccacatacc tgggcgtgaa cgtgcccgtg gagtatgtga aagacggtca
      841 gatcgtgctg aatctgtctg caagtgcgac cggcaacctg caactgacaa atgattttat
      901 ccagttcaac gcccagttta agggcgtgtc tcgtgaactg tatatcccga tgggtgccgc
      961 tctggccatt tacgctcgcg agaacggcga tggtgtgatg ttcgaaccag aagaaatcta
     1021 tgacgagctg aatattggtg gatccggagc cacgaacttc tctctgttaa agcaagcagg
     1081 agacgtggaa gaaaaccccg ggcccaccat gctggcaacc acactggaac ggatcgagaa
     1141 aaatttcgtg attactgatc cgagactgcc tgacaaccca atcatttttg cgagcgattc
     1201 cttcctgcag ctgacagaat attctcggga agagatcctg gggcgcaatt gccgttttct
     1261 gcagggaccc gagacagacc gtgccactgt tcggaaaatc agagatgcta ttgacaacca
     1321 gactgaagtg accgttcagc tgatcaatta taccaagagc ggcaagaagt tctggaacgt
     1381 gttccacctg cagccgatgc gcgattataa gggcgacgtc cagtacttca ttggcgtgca
     1441 gctggatggc accgaacgtc ttcatggcgc cgctgagcgt gaggcggtct gcctgatcaa
     1501 aaagacagcc tttcagattg ctgaggcagc gaacgacgaa aattactttt ctagaggtgg
     1561 cggggggagc gggggtggga gtaagcttgt gagcaagggc gaggaggata acatggccat
     1621 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     1681 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     1741 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     1801 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     1861 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     1921 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     1981 caacttcccc tccgacggtc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     2041 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     2101 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     2161 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     2221 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     2281 cgagctgtac aaggctagcg gaggcggtgg ctcaggcgga ggagggtcag gagggggagg
     2341 cagtggcggg ggcggctctg gaggaggggg ttctggtggt ggtagctacg taggcacccg
     2401 cgacgacgag tacgactatc tcttcaaagt tgtccttatt ggagattctg gtgttggaaa
     2461 gagtaatctc ttgtctcgat ttactcgaaa tgagtttaat ctcgaaagta agagcaccat
     2521 tggagtagag tttgcaacaa gaagcatcca ggttgatggg aaaacaataa aggcacagat
     2581 atgggacacg gcagggcagg agcgataccg agctataaca tcagcatatt atcgtggagc
     2641 tgtaggtgcc ttactggttt atgacattgc taagcatctc acatatgaaa atgtagaacg
     2701 atggctgaaa gaactgagag atcatgctga tagtaacatt gttatcatgc ttgtgggcaa
     2761 taagagtgat ttgcgtcatc tcagggcagt tcctacagat gaagcaagag cttttgcaga
     2821 aaagaatggt ttgtcattca ttgagacttc cgctctagac tctacaaatg tagaagctgc
     2881 ttttcagaca attctgacag agatataccg cattgtttcc cagaaacaaa tgtcagacag
     2941 acgtgaaaat gacatgtctc caagcaacaa tgtggttcct attcatgttc caccaaccac
     3001 tgaaaacaag ccaaaggtac aatgttgtca gaacatctaa accggtgggc ccgtttaaac
     3061 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3121 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3181 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3241 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     3301 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     3361 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3421 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3481 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3541 cctcgacccc aaaaaacttg attagggtga tggttcacgt acctagaagt tcctattccg
     3601 aagttcctat tctctagaaa gtataggaac ttccttggcc aaaaagcctg aactcaccgc
     3661 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     3721 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     3781 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     3841 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
     3901 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     3961 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
     4021 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     4081 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     4141 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     4201 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     4261 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     4321 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     4381 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
     4441 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     4501 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     4561 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     4621 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt
     4681 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     4741 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     4801 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4861 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     4921 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     4981 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     5041 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     5101 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     5161 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5221 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5281 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5341 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5401 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5461 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5521 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5581 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5641 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5701 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5761 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     5821 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5881 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5941 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6001 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6061 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6121 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6181 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6241 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6301 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6361 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6421 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6481 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6541 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6601 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6661 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6721 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6781 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6841 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6901 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6961 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7021 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7081 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     7141 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     7201 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     7261 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     7321 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
     7381 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     7441 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     7501 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     7561 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     7621 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     7681 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     7741 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     7801 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     7861 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     7921 gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag tgatagagat
     7981 cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     8041 gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag
     8101 cttgccaccg gcgcgccacc atggccgggg cttcggtgaa ggtggcggtg cgtgtccgcc
     8161 ccttcaactc ccgggaaatg agccgagact ccaagtgcat cattcagatg tctggaagca
     8221 ccaccactat tgtcaacccc aaacagccca aggagacacc caagagcttc agctttgatt
     8281 actcctactg gtcacatacc tcgccggagg acatcaacta tgcctctcag aagcaggtct
     8341 acagggacat tggggaagag atgctacagc atgcctttga gggctacaat gtgtgcatct
     8401 ttgcctatgg gcagacgggg gccggcaagt cctacaccat gatggggaaa caggagaagg
     8461 accagcaagg catcattcca cagctctgcg aagacctctt ctctcgaatc aacgacacca
     8521 ccaacgacaa catgtcctac tctgtggagg tcagctacat ggagatttac tgtgagcgag
     8581 tccgtgacct cctgaacccc aagaacaaag gcaacctgcg tgtgagggag caccctctgc
     8641 tggggcctta tgtggaggac ctctccaagc tggctgtaac ctcctacaat gacatccagg
     8701 acctcatgga ctcagggaac aaggccagga ctgtggcggc cacaaacatg aatgagacca
     8761 gcagccgttc tcacgcagtc ttcaacatca tctttaccca gaagcgccat gatgcagaga
     8821 ccaacatcac cacagagaaa gtgagcaaaa tcagcctggt ggacctggca ggcagtgaac
     8881 gagccgactc cacaggagcc aagggcacgc gactcaagga gggggccaac atcaacaagt
     8941 ctttgaccac cctgggaaag gtcatctctg ctctggctga aatggactct ggacccaaca
     9001 agaacaagaa aaagaagaag acagatttca tcccttaccg agactccgta ttgacatggc
     9061 ttctccggga aaacctcggt ggcaactcaa ggacagccat ggtggcagcc ctgagccctg
     9121 cggacatcaa ctatgatgag accctcagca cactgagata tgcagatcgg gccaaacaaa
     9181 tccgctgcaa tgccatcatc aatgaagacc ccaactctag aggtggcggg ggcagtgggg
     9241 gatcccatac tctttatgcc cccggtggat atgacattat gggatatctg tgccagatca
     9301 tgaaccggcc aaacccgcag gtggaactgg gccccgtgga tacatcctgc gccttggttc
     9361 tttgtgacct gaaacagaaa gacaccccgg tagtttacgc gagtgaagcc ttcctctaca
     9421 tgacaggtta cagcaacgca gaggtgctgg gccggaattg ccggtttctg caaagccctg
     9481 acggcatggt gaagcccaag agcacccgga agtacgtgga tagtaacaca atcaatacta
     9541 tgcgcaaggc aatcgacagg aatgccgagg tgcaggttga agtagtcaat tttaaaaaga
     9601 atggacagcg atttgttaat ttcctgacta tgatacctgt tagggacgaa acaggcgagt
     9661 atcgatactc tatgggattc cagtgcgaag tcgacgggag cgggggtggg agtaagcttg
     9721 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     9781 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     9841 agctgaccct gaagtt
//
