LOCUS       sequence-346960-       10379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prgef-1 INS-18::GFP unc-54 3' UTR C.elegans pan-neural expression of
            INS-18 fused to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 10379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH1498
FEATURES             Location/Qualifiers
     source          1..10379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     regulatory      5832..5841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5838..5840,5841..5843,5844..6557)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5838..5840,5841..5843,5844..6554)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5883..5904)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6144..6163)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6491..6512
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6582..6606)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6632..6673
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(7507..7524)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7507..7523)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7516..7538)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7736..8191
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7823..7842)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8033..8054
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8208..8227)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8327..8349
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8387..8405)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8473..8577
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8578..8646,8647..9438)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8796..8815)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9609..10197
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10098..10117
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10351..10368
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgaatg
      241 gccatgggac gtcgacaagc ttgcatgcct gcagcgtttc cgataccccc ttatatcagc
      301 acacattcag tcatcatacc acaaaatgtt gtgatagtga taggcaaaac taccgtattt
      361 cctctattaa tcttgcatgc aagactaatt ttcgattaac ccgtaggggt gcaagactaa
      421 tagagactgc aagactatta gaggctgaaa tactaatttt cgtatgctca ataattttgg
      481 aaattggcct attttttgta gaaacttgat accgtttaaa caaggaaaaa tacacacttt
      541 ttaatatttc atcaataatt tgaacgattt tgtgatttta agttcaattt gcccaaaaaa
      601 agacaatttt tctgacgtta ccgcaaagta atgctggtca ggcaaaaaga gcggtgcaaa
      661 aatataagag actgcaatac taatagagga aatacggtaa ttgaatttta gtgaaacttg
      721 cgacagtttt cctcattttt ttgttattcc ggcatacgag tgtggcatac gagtgaggtc
      781 atcttttgtt tcttccgttt cttcatcgct tttgaaaaaa aatgttgaag aaaacttcgt
      841 agccgatagc cggataatgt ttggcacggc gttccaacat atatcattgg aaatttttta
      901 agatttttgc gaaaaatcac attcttcacg atgagaacac gttattgaag gaatataaat
      961 caagaataac atatagttat atttctctat tactttaacg ttaaatatga gcaaatttga
     1021 gcattttgat tgcgatgaaa agcagaatcg agtcaactga aatccgttca aaaacctaag
     1081 ctcggttcgc ccgaaagtcg atttttccaa tagccgaaca gcactgcttc tttattttcc
     1141 aggtattttt ggggtgtaag aattatatct tctttgtttg ggtatttacg tttccgaatt
     1201 ccctttcttg atctgtcgct atttttcaga aaccagaaca gtttatcctt tttcatgaga
     1261 acctgaatag ctcaaaaacc cgtttaattt cttttcatca tctcatttga aactttccta
     1321 gtcttcttaa tgatttccag gctcctccta catttgtatc tcaagatgca tacactttat
     1381 ttccgctcca attcgaatca tctacttctc ttttttcttc aactttctac ttttttccat
     1441 tcttttcttt caaatgttcg acgtcttcaa gtgactgctc ttcattctcc tcatttttct
     1501 cgaatttcat tcttgtctct tttttgctca aaaatggaaa atgaaagtga cgtgagattt
     1561 ggacggcggg acacgggggc agtagaagca gcaaaaagga gagaaagagg acacaaataa
     1621 gaagaacgaa ttcaaaaata agcggagagg agctatttcc gtcaattcta cctccccaat
     1681 cttcatcaat tcggctcaat tgaatgacgt cacagggaga taaaccgttt ggatgagcgc
     1741 cgtccatcac tgacgccatc ccgtttggga caagaaaaga gaaaaaagag cacaaagttt
     1801 ttggtgacgg atcttgtcaa tcatatgaaa gttgttctga ttgattgtca gtttttttcc
     1861 tacttttttg gattctatcc acttctgaac ttttgacaag tttcaaactt tctgaatcat
     1921 tttctatgca ttttcctgga attcttttta tgtaaaatat gaaatagaat gtttttgaat
     1981 tcaagttctg cttttttctt cttttttgtt ctgttcgggc ttgggtatgc tttttttaaa
     2041 aattattttg cacatcgacc aataagtgcg caacttataa aattaattta tttttgttaa
     2101 tttttgaaat acttgtattg ctttaagtga tctgacctcg ccctgagctt tccacgtagt
     2161 tatcaaatac aaatcctcca agggtaacgt acctatatta ctgatcttta taataacttt
     2221 atcacctgtc cagttccaga ggaattctgt taagcttata ggcacagaag gagtcatttc
     2281 tgctggtttt taatgatcca aatctttatt tcaagtaaaa aactgaacac ttgcgaataa
     2341 aactatcaga ttaaccattc accaaaaatg tgtttgaatc taaaactttc tcagtattcc
     2401 aaatatagaa ataaataacc acgacattgc taaaatctgt ctgaattgtg tactccttac
     2461 cgtgaagtaa taatggatat aatgaatcgt ttgaaatgaa tgatcagcac atttttggtg
     2521 aaagatcaca aataaggaat aagcgacgga aaataaaacg attatttcgg atcaaaaatt
     2581 tgttgaagat catatacact cgagaccaag attattctag acaattttca aattggcttc
     2641 tttgtttgca aatcttcata ataatctgtg aagtttggaa atttgaattt ttaatctttt
     2701 ttcatagatt atagttttta tttctttgca aaactatatt aaaaaccgat gcattgtttt
     2761 agggaaatta atgagccttt tgttcaacac taaaaacaat aaaattaaaa ttttggcttc
     2821 atcatttgac cttttttaag ttcgaaaact tttctcgtat ttctgaaccg ccaatttttt
     2881 cacacatctc tagacttttg gtgcccgttc cagaaagtta agtaattgct attctaagaa
     2941 agttctcaac attgtttttt agttctgatt gaattctgat gttccaggaa tatattttaa
     3001 atttaatatt ctttgcacta tttctatact aactaaataa taaatagtct aagtatgttc
     3061 aatgagaaca aaaaagctct atctatattt ttctatccta tttcatttat atccttttca
     3121 ttttgaactc accagcttct cttcttcttc ttgtccatat acttcttaaa agtctcgaac
     3181 ttctctctct cactctccat caatttcttt atggctaccg cttgcgctct gccgcttccg
     3241 aagaaagaag gcgttggcag agctctcaat tctatttttt ctcgccgtag gctatcgatt
     3301 tctcagcttc tcttctctct tcctcactcc accaccacca cttcgggctt cttcttttct
     3361 tcattttcgt cttcttcttc atcctcttct ttttttttca gttccccctc actcctcccc
     3421 ttatatgcgc gtgcgagagg ggtgcaaaag cagcgcgcat ccgagaattg aaacgagaaa
     3481 acggagacgc agcagcagtt cgtcctcaga aaaatagcca gtaaaaagag aaaaagatag
     3541 agagagacct atcgcatttt attttcaaat tgcaattcct gcaattcatg tgtgcgtgtg
     3601 cccattttca attcttcccg ttatttttca gaccattacc aacgttcttt ctagatcttg
     3661 agacaatctt ccttctgctc aatcgttcgt cgtgaagacg gcatcgacga cgacgacgat
     3721 cagcagtaaa ggatccttat ggtccaccga cttttcatcg tccttattgc aattattctt
     3781 gtcgcaaaat caactgcaat ctcacttcaa caagctggtt tgttttcatg tttgtaatac
     3841 aaattttttt aacttttgga aaaaatatgt atcagatagc aaacttgata ataactttac
     3901 aaaatctaaa tagattcact tgggaaagtt cgacaccaat ccctgtggga ggaaaaattt
     3961 taatttccca gcacgttctc tgtgacattt tcgattcatt gtcctaacaa aatttgataa
     4021 gtttgtgaga atttcacata gttcgtaagc acttggtgga gattgcataa ctgaattgct
     4081 cacaacataa aattagcaaa ataaatgaaa acagggaaat attatatttg caagtttaat
     4141 ttctacaaga actggtttca aacatccaaa ctctttcggg gaaggcttgt cccgattttg
     4201 aaattttatc tcattctggt gtcctagaaa caattgaaaa tatgtcaagc ttctaggtag
     4261 ctggaaactc ctagctttag cttccgatat tggcacaagt acaaattcca aaaaccaaaa
     4321 aatccaactt taacgaattt ttctcattat tagtcaaaaa gagagaaagg gagagtagtt
     4381 ataggtcctt aaaaagtcca cattttatat ctcattttcc cattgtctcc tttctttctt
     4441 ttgcattcat tcctagtttg acttcaaatc ctaaatccca aaaagctcag ctttctcttg
     4501 gtttgccaag tcgactgcat tctcttagct gagatgtttt atgaatacta gataaccacc
     4561 aaacaaacta tattcttaga agaatgaggg atatcgggct ttatgacaat cataaaacat
     4621 cgacttgtga cgtggcatag ggaaaagaaa aaagagacaa gacgaacggc tcaaataaat
     4681 aaggaaacag ggaaataaca ttttttcggg agagctgttt agcgtgatta atatattgct
     4741 atcatattat tcctatcata tttacattaa aagaaacaca ttttggcaat tggcaattat
     4801 ttaattcttt ttttgaagaa acattgcttt caaatggagc actattagga gagatgtgaa
     4861 aatgaaattt aaatgaagtt ttaaattatc atacttccct catcaatact tgcatttcaa
     4921 tattaaactt tggcaactca tatcttgatt gaccaaaaaa gtgggcaaat gtttataatt
     4981 aaaaataagt aaaaaacatt ggaagattaa aaactttaga aactttttga gaaaaaaaaa
     5041 ctgaaaaatg tcacaagaaa aagtgcttcc tgtcctcgga aaaaactgat aaatgattca
     5101 aaaagtgttt caaacaatgg taaccatgga taattgagca tcttctttca gacggacgca
     5161 tgaaaatgtg cccaccaggt ggttcaacat tcacaatggc atggtcaatg tcgtgttcga
     5221 tgcgcaggag aaaacgagat gttggacgat atttcggtaa tttttttggg tagagaacta
     5281 gaacattaga gaacattaga aacctttggc cgaatatgtg agaaccagca aaatttagaa
     5341 agaattttct aatatttcct tcgaatcaaa gtgggagaca attattttgc agttgcaata
     5401 tctctaaaat caagtgttaa aaaatacata aacctgtctc cctcctgtct gacaaagaat
     5461 tttgtttgtt aattgaaaaa tgtttaaaag acgtacaaaa attgcctgaa agttgtcaat
     5521 tgaaaaaacc ttcacggacc tcttcgacca ctcgcaattt ccatgtaatt tttaacagat
     5581 caacacaaaa tatttaaata ttttagagtt acacaaattt taatttttat ataataactt
     5641 tgggtcaaga taccacaaaa aatccttttg tatagtttcc aacattatta atttccaaaa
     5701 tctttttttt tgcagaaaaa cgtgctctga tcgccccatc aatccgtcaa cttcaaacaa
     5761 tttgctgtca agttggttgc aacgtggaag atcttcttgc ctactgtgcc ccaattgggg
     5821 atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     5881 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     5941 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     6001 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     6061 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     6121 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     6181 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     6241 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     6301 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     6361 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     6421 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     6481 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     6541 acgagctgta caagtaaagc ggccgcactc gagatatcta gacccagctt tcttgtacaa
     6601 agtggttgat ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg
     6661 tctcgattct acgcgtaccg gttagtaatg agtttatctg agctccgcat cggccgctgt
     6721 catcagatcg ccatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat
     6781 ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaaact
     6841 tcttcttaat ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt
     6901 agattcaaaa atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc
     6961 cattaataat aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt
     7021 ttttacttct gataaatttt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt
     7081 atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg tctgggcctc
     7141 tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg aatttttcaa
     7201 tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttgggggt ttcccctatt
     7261 gtttgtcaag agtttcgagg acggcgtttt tcttgctaaa atcacaagta ttgatgagca
     7321 cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct cagtggaagg tgagtagaag
     7381 ttgataattt gaaagtggag tagtgtctat ggggtttttg ccttaaatga cagaatacat
     7441 tcccaatata ccaaacataa ctgtttccta ctagtggcca cgtgggccgt gcaccttaag
     7501 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     7561 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     7621 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     7681 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     7741 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7801 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7861 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7921 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     7981 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8041 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     8101 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8161 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     8221 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     8281 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     8341 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     8401 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     8461 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     8521 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     8581 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     8641 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8701 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8761 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     8821 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8881 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8941 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     9001 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     9061 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9121 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9181 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9241 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     9301 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     9361 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     9421 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     9481 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     9541 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     9601 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     9661 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     9721 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     9781 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     9841 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9901 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9961 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    10021 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    10081 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    10141 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    10201 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    10261 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    10321 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaaga
//
