LOCUS       sequence-346962-       11776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the dead version of humanized 
            fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer
            sequence is replaced by the type IIS restriction site for 
            endonucleas.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 11776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_dfnCas12a-KRAB_crRNA NC
FEATURES             Location/Qualifiers
     source          1..11776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     ncRNA           complement(1491..1558)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(1606..1642)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             2639..2650
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2871..2891
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6840..7034
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             7716..7742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      7744..7753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7758..7778
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8460..8469
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     rep_origin      complement(9974..10562)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10054..10073)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10733..11524,11525..11593))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11356..11375
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11594..11698)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(11735..11776,1..462)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 taaatatata aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt
       61 tttccgaaga tgtaaaagac tctaggggga tcgccaacaa atactacctt ttatcttgta
      121 cttcctgctc tcaggtatta atgccgaatt gtttcatctt gtctgtgtag aagaccacac
      181 acgaaaatcc tgtgatttta cattttactt atcgttaatc gaatgtatat ctatttaatc
      241 tgcttttctt gtctaataaa tatatatgta aagtacgctt tttgttgaaa ttttttaaac
      301 ctttgtttat ttttttttct tcattccgta actcttctac cttctttatt tactttctaa
      361 aatccaaata caaaacataa aaataaataa acacagagta aattcccaaa ttattccatc
      421 attaaaagat acgaggcgcg tgtaagttac aggcaagcga tccgtctaag aaaccggtgc
      481 aaagctgacg cccttttcgt cgtcgttgct ggtgttaaaa ttgcgcgttt cttttttttc
      541 cgtctttgtc gtcttttgac ttttttatct tcttttctgt cttcttttat tcttctcttc
      601 ttcttcatca ctccctcttc atttttcttc ttctttttcc tcgtctattg tcctcgggca
      661 ttaccagttg tgctgctcac gtgcgcgcaa gcggtgaaag ggtgagagga tacacggaca
      721 acaatcattg aaaattacgc ttaaagccgg tgcctggaaa agtccacgcg cagatggcct
      781 ttgcttgcct tgctgctgct gcagctgctg ctgctgtcgc ctgtgatggt aatggttgtt
      841 gttgtggtcc tgcttgcttg ctagtttgca ttgcgtttgc ggttgcctta tgaccgactc
      901 cgtgtgattg atccttaaac atattttatc attgatgtgg attgtgtgac gacggcaaaa
      961 aagaattact ttacacatga ctatcacacc tgctcccctc ccttctcctt tcaatcggtc
     1021 aacttcccaa ccgctcctcg aaaatatttc agttgaatcc tctactacgt accccttact
     1081 taaatttact ttctctctcc ccatcaatgc gccgttgttc tgaagttgta ttattgatgg
     1141 gagatgattg gcactgcacc agacctggcc aaaagccacc acaggtggcc acatactctt
     1201 gcatgggtat actctaccga ggcaaagatt aaagtgcacg tcccctgccc ctctctctcc
     1261 ctacttcctt cagtctcagt ggttccgtgc ccgttcagca ctacatcgcc ctgcccgctg
     1321 ttccttctca aacagccacc catcccaacc cacatacaca gaaacaacct ttcctacaca
     1381 ttttcccaag tcttctcttc actattctac actaatacac caccgtgata tgccggttat
     1441 ctagatgtcc tttctccttt ctcatccttt ctttcccatg gtacccagga gtcccattcg
     1501 ccatgccgaa gcatgttgcc cagccggcgc cagcgaggag gctgggacca tgccggcccc
     1561 agctggatac tagcgtcatg ggatccatct acaacagtag aaattgacga gcttactcgt
     1621 ttcgtcctca cggactcatc agaatttcga agagccaagg cctgctcttc atcgattctg
     1681 tggatggagg gagggaagga gggggggagt gagtgagtcg agagaacgac gactggggca
     1741 aagagaggat gacgccactg caacaaaaga cagggaagaa tagaggctgt tggaaaacag
     1801 gaacacagtg aagaatagag gctgcagggt aacaggcaca tttatcgcag agaggcgggc
     1861 ggcaggggcg atacgtaaag agacccctaa acttctccgc gtcatccgtt cacgctacct
     1921 ccttccctcc ctttactccc attgatcacc aaggcagcgc ccgcagccac cagcagccac
     1981 cgtcctcagt gtcacagcta gacgcgtccc catcaggcag cagaccacca gaatatgcgc
     2041 tttcgcttcc catcatatcc ctccctcctc cccttctatt cctcatcatg catcatttct
     2101 ttctccttgc tgcatgcccc tcttcttacc ttggctaaga tctaccggag gaagaagtct
     2161 tcaacgatag ataaatgcct gtatcgtgat ggttgtttga atggtggaag tgagccaggt
     2221 attgagaacg aggggcgcaa gggtgcgtgg aagaaggcag ggagaggcga ggcgaaggag
     2281 aagggcatgt ggtgggaacg aagcctccac accacagcca cccatgcttt tctctgttcg
     2341 agttcccggg ttctactctc acacactaca cgtgcacaga aaacattcac gcacaacaga
     2401 gatacagagg gatccgtcga gatggtctac acgtcctaat gtcccctgct ttcgaagccc
     2461 agagccatcg ctttctgttt ttatccattc gcaacacatc ctgttctcgc gacctctcct
     2521 cttcctggcc accatcgctt ctccgtgcca tgccctgtac atccttccct ccctcgttgt
     2581 ctaccacact aatcatgcgc tctgcagcag acactcggct actatttgtg tcgtacggat
     2641 tgaggggagg gaggggaagg gaggcaagaa gtaaatcggt ggtcaacaac agacgcggtc
     2701 tccccaccct ccctcattcc tttcccctcc tataatgagc gaagaaagcc atggacaccg
     2761 agtcacccac tctcctaatc caccaccacc agggcactaa cggactcacg ctcaccaaac
     2821 tcggtaacaa tatccactgc acacagatac acacgcaacc ctcgagcatg cccaagaaaa
     2881 agcggaaggt gagcatctac caggagttcg tcaacaagta ttcactgagt aagacactgc
     2941 ggttcgagct gatcccacag ggcaagacac tggagaacat caaggcccga ggcctgattc
     3001 tggacgatga gaagcgggca aaagactata agaaagccaa gcagatcatt gataaatacc
     3061 accagttctt tatcgaggaa attctgagct ccgtgtgcat cagtgaggat ctgctgcaga
     3121 attactcaga cgtgtacttc aagctgaaga agagcgacga tgacaacctg cagaaggact
     3181 tcaagtccgc caaggacacc atcaagaaac agattagcga gtacatcaag gactccgaaa
     3241 agtttaaaaa tctgttcaac cagaatctga tcgatgctaa gaaaggccag gagtccgacc
     3301 tgatcctgtg gctgaaacag tctaaggaca atgggattga actgttcaag gctaactccg
     3361 atatcactga tattgacgag gcactggaaa tcatcaagag cttcaaggga tggaccacat
     3421 actttaaagg cttccacgag aaccgcaaga acgtgtactc cagcaacgac attcctacct
     3481 ccatcatcta ccgaatcgtc gatgacaatc tgccaaagtt cctggagaac aaggccaaat
     3541 atgaatctct gaaggacaaa gctcccgagg caattaatta cgaacagatc aagaaagatc
     3601 tggctgagga actgacattc gatatcgact ataagactag cgaggtgaac cagagggtct
     3661 tttccctgga cgaggtgttt gaaatcgcca atttcaacaa ttacctgaac cagtccggca
     3721 ttactaaatt caataccatc attggcggga agtttgtgaa cggggagaat accaagcgca
     3781 agggaattaa cgaatacatc aatctgtata gccagcagat caacgacaaa actctgaaga
     3841 aatacaagat gtctgtgctg ttcaaacaga tcctgagtga taccgagtcc aagtcttttg
     3901 tcattgataa actggaagat gactcagacg tggtcactac catgcagagc ttttatgagc
     3961 agatcgccgc tttcaagaca gtggaggaaa aatctattaa ggaaactctg agtctgctgt
     4021 tcgatgacct gaaagcccag aagctggacc tgagtaagat ctacttcaaa aacgataaga
     4081 gtctgacaga cctgtcacag caggtgtttg atgactattc cgtgattggg accgccgtcc
     4141 tggagtacat tacacagcag atcgctccaa agaacctgga taatccctct aagaaagagc
     4201 aggaactgat cgctaagaaa accgagaagg caaaatatct gagtctggaa acaattaagc
     4261 tggcactgga ggagttcaac aagcacaggg atattgacaa acagtgccgc tttgaggaaa
     4321 tcctggccaa cttcgcagcc atccccatga tttttgatga gatcgcccag aacaaagaca
     4381 atctggctca gatcagtatt aagtaccaga accagggcaa gaaagacctg ctgcaggctt
     4441 cagcagaaga tgacgtgaaa gccatcaagg atctgctgga ccagaccaac aatctgctgc
     4501 acaagctgaa aatcttccat attagtcagt cagaggataa ggctaatatc ctggataaag
     4561 acgaacactt ctacctggtg ttcgaggaat gttacttcga gctggcaaac attgtccccc
     4621 tgtataacaa gattaggaac tacatcacac agaagcctta ctctgacgag aagtttaaac
     4681 tgaacttcga aaatagtacc ctggccaacg ggtgggataa gaacaaggag cctgacaaca
     4741 cagctatcct gttcatcaag gatgacaagt actatctggg agtgatgaat aagaaaaaca
     4801 ataagatctt cgatgacaaa gccattaagg agaacaaagg ggaaggatac aagaaaatcg
     4861 tgtataagct gctgcccggc gcaaataaga tgctgcctaa ggtgttcttc agcgccaaga
     4921 gtatcaaatt ctacaaccca tccgaggaca tcctgcggat tagaaatcac tcaacacata
     4981 ctaagaacgg gagcccccag aagggatatg agaaatttga gttcaacatc gaggattgca
     5041 ggaagtttat tgacttctac aagcagagca tctccaaaca ccctgaatgg aaggattttg
     5101 gcttccggtt ttccgacaca cagagatata actctatcga cgagttctac cgcgaggtgg
     5161 aaaatcaggg gtataagctg acttttgaga acatttctga aagttacatc gacagcgtgg
     5221 tcaatcaggg aaagctgtac ctgttccaga tctataacaa agatttttca gcatacagca
     5281 agggcagacc aaacctgcat acactgtact ggaaggccct gttcgatgag aggaatctgc
     5341 aggacgtggt ctataaactg aacggagagg ccgaactgtt ttaccggaag cagtctattc
     5401 ctaagaaaat cactcaccca gctaaggagg ccatcgctaa caagaacaag gacaatccta
     5461 agaaagagag cgtgttcgaa tacgatctga ttaaggacaa gcggttcacc gaagataagt
     5521 tctttttcca ttgtccaatc accattaact tcaagtcaag cggcgctaac aagttcaacg
     5581 acgagatcaa tctgctgctg aaggaaaaag caaacgatgt gcacatcctg agcattgccc
     5641 gaggagagcg gcatctggcc tactataccc tggtggatgg caaagggaat atcattaagc
     5701 aggatacatt caacatcatt ggcaatgacc ggatgaaaac caactaccac gataaactgg
     5761 ctgcaatcga gaaggataga gactcagcta ggaaggactg gaagaaaatc aacaacatta
     5821 aggagatgaa ggaaggctat ctgagccagg tggtccatga gattgcaaag ctggtcatcg
     5881 aatacaatgc cattgtggtg ttcgcggatc tgaacttcgg ctttaagagg gggcgcttta
     5941 aggtggaaaa acaggtctat cagaagctgg agaaaatgct gatcgaaaag ctgaattacc
     6001 tggtgtttaa agataacgag ttcgacaaga ccggaggcgt cctgagagcc taccagctga
     6061 cagctccctt tgaaactttc aagaaaatgg gaaaacagac aggcatcatc tactatgtgc
     6121 cagccggatt cacttccaag atctgccccg tgaccggctt tgtcaaccag ctgtacccta
     6181 aatatgagtc agtgagcaag tcccaggaat ttttcagcaa gttcgataag atctgttata
     6241 atctggacaa ggggtacttc gagttttcct tcgattacaa gaacttcggc gacaaggccg
     6301 ctaaggggaa atggaccatt gcctccttcg gatctcgcct gatcaacttt cgaaattccg
     6361 ataaaaacca caattgggac actagggagg tgtacccaac caaggagctg gaaaagctgc
     6421 tgaaagacta ctctatcgag tatggacatg gcgaatgcat caaggcagcc atctgtggcg
     6481 agagtgataa gaaatttttc gccaagctga cctcagtgct gaatacaatc ctgcagatgc
     6541 ggaactcaaa gaccgggaca gaactggact atctgattag ccccgtggct gatgtcaacg
     6601 gaaacttctt cgacagcaga caggcaccca aaaatatgcc tcaggatgca gacgccaacg
     6661 gggcctacca catcgggctg aagggactga tgctgctggg ccggatcaag aacaatcagg
     6721 aggggaagaa gctgaacctg gtcattaaga acgaggaata cttcgagttt gtccagaata
     6781 gaaataacgg gtccggcagc gggtccgtta acgacgcgaa gtctttgaca gcctggagca
     6841 gaacgttggt cacatttaag gatgttttcg tggactttac tcgtgaggag tggaaactac
     6901 ttgacactgc ccagcaaatt gtttacagga acgttatgtt ggaaaactac aaaaatttag
     6961 tttcccttgg ttatcaactg accaagccag atgtcatact gagattagag aaaggcgaag
     7021 aaccctggct agttgagagg gaaattcatc aagaaaccca ccctgattct gaaacagctt
     7081 ttgaaattaa atcttcagtc tcttctcgta gcatttttaa ggacaagcag tcttgcgata
     7141 tcaagatgga gggtatggcc cgtaacgact tgtgggggtc cggcagcggg tccgttaaca
     7201 tggtgtttac tctcgaggac ttcgtgggcg actggcggca gacagctggc tataacctcg
     7261 accaggtgct ggagcagggc ggtgtgtcct cgcttttcca gaacctgggc gtctccgtga
     7321 ccccaatcca gcgcatcgtc ctgagcggcg agaacggcct gaagatcgac atccacgtca
     7381 tcattcccta cgagggcctg agcggggacc aaatgggcca gatcgagaag atcttcaagg
     7441 ttgtgtaccc cgtggacgat caccacttca aggtgatact gcactacggg acgctggtca
     7501 tcgacggtgt gacgccgaac atgattgact acttcggccg tccttacgag ggcattgccg
     7561 tgttcgacgg caagaagatc acggtgaccg gcaccctgtg gaacggcaat aagatcattg
     7621 acgagcggct gatcaacccg gatggctcgc tgttgttccg cgtgaccatc aacggggtaa
     7681 ccggctggcg cctgtgcgag cgcatcctgg ccggataccc ctacgacgtg cccgactacg
     7741 cgtccatggt ggctagcccg aaaaagaaga ggaaggtctg acaattggaa agatccaaga
     7801 gagacgagta gagatttttt ttttgggatt gatgtttgtc gttctttgag ttgtcgtcga
     7861 gttacgcctt ttgtaagaat gttccgcagg agaggaggag gatgggcatg agtgagggtg
     7921 agagggcttg cccgcttttt ttttttaaaa acgctgaaga cgtggttgtc aaacaaaccc
     7981 cccatagaaa cgattttgtt acggtgcggt ccagacgtca cttgaatggc tccgcggaaa
     8041 ggccagggag ggaagggggg agggaggaaa catgaaacat gttgaacggc tcaacagggt
     8101 ttgggggaca agagaggtag cgccctgatg gactgctccc tcccctcctt tccctcaatg
     8161 tctcattcat ccatgcttcc cccttctctc tctcccctcc gttccatccc ccgcgggcgt
     8221 ggtagtggcg tgatgggatc cactaaaatg tacgtgtaag aaaagccggt gagcttacgc
     8281 ttttgtgaaa gtgggagtac gagtgttgtg tgtgtgtgta gtggtttcag accccagaca
     8341 gaggcgaagc agaaaaagca gacgatgaag acgacgaaga aatgagcagt ctatttttat
     8401 cgtggaaaca gaagaggtga tatcgtctcc tgcaggcatg caagctgatc cactagaggc
     8461 catggcggcc gcgatggagt ggatggagga ggaggcgagc gtagcagcaa gcgtgagtta
     8521 tacagccagg cacatgtcgc aatccttcgg tctcgggctt aaaatccacg cactaatcac
     8581 gctgggccat gcaaagagca atgccgaggc ccaccacaca aaacgctgtg tcgcgcgttg
     8641 cggcctgaag cttcatactt cttagtcgcc gccaaaaggg ctcgagagac gagacccgtt
     8701 ggcatgaccg atgttgttcg acgcggtttg cttcgtcaca gtcgacgtga ttcaggaatc
     8761 tggagcctgc agatcatttt tttcagcctg atatcgttct tttccactga gaaccatcag
     8821 accacctttt cttccattgt gtgaaggagt aggagttgcc gtgctgcttt gtgggagaca
     8881 tctgcgatgg tgaccagcct cccgtcgtct ggtcgacgtg acgagcctct tcactgttct
     8941 tcgacggaga gacgcaagcg agacggctct agaccttttg gacacgcatt ctgtgtgtga
     9001 actagtggac agtgatacca cgtctgaaag ctcaccactg cccatggtgc agctacttgt
     9061 cacaaagttt tgactccgtc ggtatcacca ttcgcgctcg tgtgcctggt tgttccgcca
     9121 cgccggcctg ccccggggcg gggcaatatt ctaaaatctc acgcaaaaca ccgcacttac
     9181 ccctcacaca tattcgtgat agaccaccac caatctcagc ccgcatcaac acaggagggc
     9241 ccatgcccct ctcgcaggag gagagcacgc tgatcgagag ggctacggcg acgattaact
     9301 cgattcctat ctccgaggat tactcggtgg cttccgctgc tctgtcgtcc gacggacgga
     9361 tcttcacggg ggtcaacgtg taccatttta cgggggggcc ttgcgcggag ctggtggtcc
     9421 tggggaccgc cgctgctgcc gcggctggga acctgacgtg cattgtcgcc atcgggaacg
     9481 agaaccgcgg gattctgagc ccctgcgggc ggtgccggca ggtcctgctc gacctccacc
     9541 ctgggatcaa ggcgattgtg aaggattccg acgggcagcc tacggcggtc ggtatcagag
     9601 aacttctccc ttcgggctac gtgtgggaga gcgcttgaac tagtagtaga tgccgaccgg
     9661 agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa
     9721 taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg
     9781 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
     9841 atttctaatt cctaaaacca aaatccagtg acctggttta aaccacagaa tcaggggata
     9901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     9961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    10021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    10081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    10141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    10201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    10261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    10321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    10381 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    10441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    10501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    10561 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    10621 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    10681 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    10741 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10801 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    10861 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    10921 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10981 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    11041 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    11101 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    11161 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    11221 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    11281 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    11341 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    11401 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    11461 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11521 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11581 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11641 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    11701 catttccccg aaaagtgcca cctgggtcct tttcatcacg tgctataaaa ataattataa
    11761 tttaaatttt ttaata
//
