LOCUS       sequence-346964-       12102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the humanized fnCas12a gene sequence
            tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the
            Nitrate reductase gene of N. oceanica IMET1 separated by full.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 12102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_hfnCas12a_NR1_NR2
FEATURES             Location/Qualifiers
     source          1..12102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        527..631
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(632..700,701..1492)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(850..869)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1663..2251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2152..2171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3211..3585
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      4470..4479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5160..5180)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5185..5194
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5196..5222)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(9660..9680)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9901..9912)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    join(12089..12102,1..490)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 aacttacacg cgcctcgtat cttttaatga tggaataatt tgggaattta ctctgtgttt
       61 atttattttt atgttttgta tttggatttt agaaagtaaa taaagaaggt agaagagtta
      121 cggaatgaag aaaaaaaaat aaacaaaggt ttaaaaaatt tcaacaaaaa gcgtacttta
      181 catatatatt tattagacaa gaaaagcaga ttaaatagat atacattcga ttaacgataa
      241 gtaaaatgta aaatcacagg attttcgtgt gtggtcttct acacagacaa gatgaaacaa
      301 ttcggcatta atacctgaga gcaggaagta caagataaaa ggtagtattt gttggcgatc
      361 cccctagagt cttttacatc ttcggaaaac aaaaactatt ttttctttaa tttctttttt
      421 tactttctat ttttaattta tatatttata ttaaaaaatt taaattataa ttatttttat
      481 agcacgtgat gaaaaggacc caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      541 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      601 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      661 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      721 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      781 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      841 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      901 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      961 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     1021 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     1081 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1141 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     1201 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     1261 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1321 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     1381 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1441 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     1501 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1561 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1621 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1681 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1741 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1801 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1861 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1921 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1981 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2041 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2101 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2161 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2221 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2281 cctggccttt tgctggcctt ttgctcacat gtttaaacgg ggtcgtttat ttctctgcca
     2341 gccttggacc tcgtggttgg gcgtttcctc ctccttggtg cactgaacct gtccgcatcc
     2401 tgcgtcggtt tggcggtctt ttgtcctttc ctctatagcc cgcccgtcta gagggcacac
     2461 gcgatgatct ttatatctct tcatgtgtct ttgttttaac taggatactg ccgggtgaat
     2521 gcccatcaga caagaggcca aactctatct acaccctttt gacttctgtt gtggtcgtag
     2581 tgtgtgcttg catgccctga aagtccaggc atcccacttg tgctctaacc ccattcaaaa
     2641 cagcagaagt gcttaattaa gatatagatt catgatctcc tgtcccctcc ttcttacctt
     2701 ttcacaaacc tcacacagaa gtctccactc ttcgcctcta aaacctcttt ttaaattatg
     2761 gtaagttcgt gcggcagtgg gttttcggat ctatatttgt caagatccag ttcaaggtca
     2821 gggatgtaga ttaagtacag aaggagaagc acaagcgcgc cagttcgccc ctcacggcct
     2881 ggagcagggc atttaatccc tctatcttac cagaaccata ctatacaacc aatcctgttg
     2941 gcatcgctct gtctatttgt cgtgcgtgca tgtgtccatg gtgtggtggg gggcaggggt
     3001 tttcggggtt gcggttgaag gcaccttatc agaaagatgc cctcagagat agaggtagcc
     3061 ccctcccccc gatcttcgac cagtcctgtc aggcgaacac tttcacccgt cgttcacctc
     3121 gttacacaca aggagtagac ctctgaagtt ctaattgtca taaatgcccc tcccccctcc
     3181 ctccttccct tcatcctccc ctccgagcag atggccaagt tgaccagtgc cgttccggtg
     3241 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     3301 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     3361 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     3421 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     3481 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     3541 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgatggcc agggatcagg
     3601 aggagggagt gaagaggaga agggatctgg tttcagagat ccccacttct gccgtcgtct
     3661 ttcggccttc cttcctttta ggtgtcatgc cttaggtcct tcaagtcctc acctgtcgtc
     3721 gtcatgtgtg tgtgtgcccg tcatacaagt cactcgatcc aattcacgca tcggttcaat
     3781 caaaataaga ctagaccccg agggaagaag ggcagaagga aatcgaaggg gtgggatgtg
     3841 tgtgagagag ggaaggagaa atgaaagaag tgaacaatgt catggtagcc agtaaggaga
     3901 gagtagaagc gaagaaagca aaagcactgt tgtgaagaaa cgaaatggaa gatggtcatc
     3961 gctcctggct ctacttgtgg tttttctatc tttaatttca ggcgtcctgg tctcgttaca
     4021 tcagctccct tatctcattg gtttatcccc tactctactg ctgcttcttc cttccatccg
     4081 tgactgtata acaacgaatt gtagtaccgc agatagacaa tagaaaaatg ccaaaaaagg
     4141 catcattgat ttgctcctcc ccattaagtc actgtacgcc accatcgcca ctaccctccc
     4201 ttagcaaata gtctttattt agaacacaga agaagagtac gttgtgtgca tgcgtgttgt
     4261 tcgtacaact tccgtgtcag gacgagagac catttcaaca gtgagtaatt ccgcctgcgt
     4321 ttattttttc ctctttttcc ttttcttgtt ttcttccttt gtacgtgtat ttccctctct
     4381 tccacgtctt ctttatcgtc atcttgtctg ccatccttac tctatcttct accctcactc
     4441 acttccacac tctcaagtcc accctcgcgg ccgccatggc ctctagtgga tcagcttgca
     4501 tgcctgcagg agacgatatc acctcttctg tttccacgat aaaaatagac tgctcatttc
     4561 ttcgtcgtct tcatcgtctg ctttttctgc ttcgcctctg tctggggtct gaaaccacta
     4621 cacacacaca caacactcgt actcccactt tcacaaaagc gtaagctcac cggcttttct
     4681 tacacgtaca ttttagtgga tcccatcacg ccactaccac gcccgcgggg gatggaacgg
     4741 aggggagaga gagaaggggg aagcatggat gaatgagaca ttgagggaaa ggaggggagg
     4801 gagcagtcca tcagggcgct acctctcttg tcccccaaac cctgttgagc cgttcaacat
     4861 gtttcatgtt tcctccctcc ccccttccct ccctggcctt tccgcggagc cattcaagtg
     4921 acgtctggac cgcaccgtaa caaaatcgtt tctatggggg gtttgtttga caaccacgtc
     4981 ttcagcgttt ttaaaaaaaa aagcgggcaa gccctctcac cctcactcat gcccatcctc
     5041 ctcctctcct gcggaacatt cttacaaaag gcgtaactcg acgacaactc aaagaacgac
     5101 aaacatcaat cccaaaaaaa aaatctctac tcgtctctct tggatctttc caattgtcag
     5161 accttcctct tctttttcgg gctagccacc atggacgcgt agtcgggcac gtcgtagggg
     5221 tatccggcca ggatgcgctc gcacaggcgc cagccggtta ccccgttgat ggtcacgcgg
     5281 aacaacagcg agccatccgg gttgatcagc cgctcgtcaa tgatcttatt gccgttccac
     5341 agggtgccgg tcaccgtgat cttcttgccg tcgaacacgg caatgccctc gtaaggacgg
     5401 ccgaagtagt caatcatgtt cggcgtcaca ccgtcgatga ccagcgtccc gtagtgcagt
     5461 atcaccttga agtggtgatc gtccacgggg tacacaacct tgaagatctt ctcgatctgg
     5521 cccatttggt ccccgctcag gccctcgtag ggaatgatga cgtggatgtc gatcttcagg
     5581 ccgttctcgc cgctcaggac gatgcgctgg attggggtca cggagacgcc caggttctgg
     5641 aaaagcgagg acacaccgcc ctgctccagc acctggtcga ggttatagcc agctgtctgc
     5701 cgccagtcgc ccacgaagtc ctcgagagta aacaccatgt taacggaccc gctgccggac
     5761 ccgttatttc tattctggac aaactcgaag tattcctcgt tcttaatgac caggttcagc
     5821 ttcttcccct cctgattgtt cttgatccgg cccagcagca tcagtccctt cagcccgatg
     5881 tggtaggccc cgttggcgtc tgcatcctga ggcatatttt tgggtgcctg tctgctgtcg
     5941 aagaagtttc cgttgacatc agccacgggg ctaatcagat agtccagttc tgtcccggtc
     6001 tttgagttcc gcatctgcag gattgtattc agcactgagg tcagcttggc gaaaaatttc
     6061 ttatcactct cgccacagat ggctgccttg atgcattcgc catgtccata ctcgatagag
     6121 tagtctttca gcagcttttc cagctccttg gttgggtaca cctccctagt gtcccaattg
     6181 tggtttttat cggaatttcg aaagttgatc aggcgagatc cgaaggaggc aatggtccat
     6241 ttccccttag cggccttgtc gccgaagttc ttgtaatcga aggaaaactc gaagtacccc
     6301 ttgtccagat tataacagat cttatcgaac ttgctgaaaa attcctggga cttgctcact
     6361 gactcatatt tagggtacag ctggttgaca aagccggtca cggggcagat cttggaagtg
     6421 aatccggctg gcacatagta gatgatgcct gtctgttttc ccattttctt gaaagtttca
     6481 aagggagctg tcagctggta ggctctcagg acgcctccgg tcttgtcgaa ctcgttatct
     6541 ttaaacacca ggtaattcag cttttcgatc agcattttct ccagcttctg atagacctgt
     6601 ttttccacct taaagcgccc cctcttaaag ccgaagttca gatcctcgaa caccacaatg
     6661 gcattgtatt cgatgaccag ctttgcaatc tcatggacca cctggctcag atagccttcc
     6721 ttcatctcct taatgttgtt gattttcttc cagtccttcc tagctgagtc tctatccttc
     6781 tcgattgcag ccagtttatc gtggtagttg gttttcatcc ggtcattgcc aatgatgttg
     6841 aatgtatcct gcttaatgat attccctttg ccatccacca gggtatagta ggccagatgc
     6901 cgctctcctc ggtcaatgct caggatgtgc acatcgtttg ctttttcctt cagcagcaga
     6961 ttgatctcgt cgttgaactt gttagcgccg cttgacttga agttaatggt gattggacaa
     7021 tggaaaaaga acttatcttc ggtgaaccgc ttgtccttaa tcagatcgta ttcgaacacg
     7081 ctctctttct taggattgtc cttgttcttg ttagcgatgg cctccttagc tgggtgagtg
     7141 attttcttag gaatagactg cttccggtaa aacagttcgg cctctccgtt cagtttatag
     7201 accacgtcct gcagattcct ctcatcgaac agggccttcc agtacagtgt atgcaggttt
     7261 ggtctgccct tgctgtatgc tgaaaaatct ttgttataga tctggaacag gtacagcttt
     7321 ccctgattga ccacgctgtc gatgtaactt tcagaaatgt tctcaaaagt cagcttatac
     7381 ccctgatttt ccacctcgcg gtagaactcg tcgatagagt tatatctctg tgtgtcggaa
     7441 aaccggaagc caaaatcctt ccattcaggg tgtttggaga tgctctgctt gtagaagtca
     7501 ataaacttcc tgcaatcctc gatgttgaac tcaaatttct catatccctt ctgggggctc
     7561 ccgttcttag tatgtgttga gtgatttcta atccgcagga tgtcctcgga tgggttgtag
     7621 aatttgatac tcttggcgct gaagaacacc ttaggcagca tcttatttgc gccgggcagc
     7681 agcttataca cgattttctt gtatccttcc cctttgttct ccttaatggc tttgtcatcg
     7741 aagatcttat tgtttttctt attcatcact cccagatagt acttgtcatc cttgatgaac
     7801 aggatagctg tgttgtcagg ctccttgttc ttatcccacc cgttggccag ggtactattt
     7861 tcgaagttca gtttaaactt ctcgtcagag taaggcttct gtgtgatgta gttcctaatc
     7921 ttgttataca gggggacaat gtttgccagc tcgaagtaac attcctcgaa caccaggtag
     7981 aagtgttcgt ctttatccag gatattagcc ttatcctctg actgactaat atggaagatt
     8041 ttcagcttgt gcagcagatt gttggtctgg tccagcagat ccttgatggc tttcacgtca
     8101 tcttctgctg aagcctgcag caggtctttc ttgccctggt tctggtactt aatactgatc
     8161 tgagccagat tgtctttgtt ctgggcgatc tcatcaaaaa tcatggggat ggctgcgaag
     8221 ttggccagga tttcctcaaa gcggcactgt ttgtcaatat ccctgtgctt gttgaactcc
     8281 tccagtgcca gcttaattgt ttccagactc agatattttg ccttctcggt tttcttagcg
     8341 atcagttcct gctctttctt agagggatta tccaggttct ttggagcgat ctgctgtgta
     8401 atgtactcca ggacggcggt cccaatcacg gaatagtcat caaacacctg ctgtgacagg
     8461 tctgtcagac tcttatcgtt tttgaagtag atcttactca ggtccagctt ctgggctttc
     8521 aggtcatcga acagcagact cagagtttcc ttaatagatt tttcctccac tgtcttgaaa
     8581 gcggcgatct gctcataaaa gctctgcatg gtagtgacca cgtctgagtc atcttccagt
     8641 ttatcaatga caaaagactt ggactcggta tcactcagga tctgtttgaa cagcacagac
     8701 atcttgtatt tcttcagagt tttgtcgttg atctgctggc tatacagatt gatgtattcg
     8761 ttaattccct tgcgcttggt attctccccg ttcacaaact tcccgccaat gatggtattg
     8821 aatttagtaa tgccggactg gttcaggtaa ttgttgaaat tggcgatttc aaacacctcg
     8881 tccagggaaa agaccctctg gttcacctcg ctagtcttat agtcgatatc gaatgtcagt
     8941 tcctcagcca gatctttctt gatctgttcg taattaattg cctcgggagc tttgtccttc
     9001 agagattcat atttggcctt gttctccagg aactttggca gattgtcatc gacgattcgg
     9061 tagatgatgg aggtaggaat gtcgttgctg gagtacacgt tcttgcggtt ctcgtggaag
     9121 cctttaaagt atgtggtcca tcccttgaag ctcttgatga tttccagtgc ctcgtcaata
     9181 tcagtgatat cggagttagc cttgaacagt tcaatcccat tgtccttaga ctgtttcagc
     9241 cacaggatca ggtcggactc ctggcctttc ttagcatcga tcagattctg gttgaacaga
     9301 tttttaaact tttcggagtc cttgatgtac tcgctaatct gtttcttgat ggtgtccttg
     9361 gcggacttga agtccttctg caggttgtca tcgtcgctct tcttcagctt gaagtacacg
     9421 tctgagtaat tctgcagcag atcctcactg atgcacacgg agctcagaat ttcctcgata
     9481 aagaactggt ggtatttatc aatgatctgc ttggctttct tatagtcttt tgcccgcttc
     9541 tcatcgtcca gaatcaggcc tcgggccttg atgttctcca gtgtcttgcc ctgtgggatc
     9601 agctcgaacc gcagtgtctt actcagtgaa tacttgttga cgaactcctg gtagatgctc
     9661 accttccgct ttttcttggg catgctcgag ggttgcgtgt gtatctgtgt gcagtggata
     9721 ttgttaccga gtttggtgag cgtgagtccg ttagtgccct ggtggtggtg gattaggaga
     9781 gtgggtgact cggtgtccat ggctttcttc gctcattata ggaggggaaa ggaatgaggg
     9841 agggtgggga gaccgcgtct gttgttgacc accgatttac ttcttgcctc ccttcccctc
     9901 cctcccctca atccgtacga cacaaatagt agccgagtgt ctgctgcaga gcgcatgatt
     9961 agtgtggtag acaacgaggg agggaaggat gtacagggca tggcacggag aagcgatggt
    10021 ggccaggaag aggagaggtc gcgagaacag gatgtgttgc gaatggataa aaacagaaag
    10081 cgatggctct gggcttcgaa agcaggggac attaggacgt gtagaccatc tcgacggatc
    10141 cctctgtatc tctgttgtgc gtgaatgttt tctgtgcacg tgtagtgtgt gagagtagaa
    10201 cccgggaact cgaacagaga aaagcatggg tggctgtggt gtggaggctt cgttcccacc
    10261 acatgccctt ctccttcgcc tcgcctctcc ctgccttctt ccacgcaccc ttgcgcccct
    10321 cgttctcaat acctggctca cttccaccat tcaaacaacc atcacgatac aggcatttat
    10381 ctatcgttga agacttcttc ctccggtaga tcttagccaa ggtaagaaga ggggcatgca
    10441 gcaaggagaa agaaatgatg catgatgagg aatagaaggg gaggagggag ggatatgatg
    10501 ggaagcgaaa gcgcatattc tggtggtctg ctgcctgatg gggacgcgtc tagctgtgac
    10561 actgaggacg gtggctgctg gtggctgcgg gcgctgcctt ggtgatcaat gggagtaaag
    10621 ggagggaagg aggtagcgtg aacggatgac gcggagaagt ttaggggtct ctttacgtat
    10681 cgcccctgcc gcccgcctct ctgcgataaa tgtgcctgtt accctgcagc ctctattctt
    10741 cactgtgttc ctgttttcca acagcctcta ttcttccctg tcttttgttg cagtggcgtc
    10801 atcctctctt tgccccagtc gtcgttctct cgactcactc actccccccc tccttccctc
    10861 cctccatcca cagaatcgat gaagagcagg ccttggctct tcgtctaaga actttaaata
    10921 atttctactg ttgtagatcc acttccacga caatcgcgtc ctgtgctggt ctaagaactt
    10981 taaataattt ctactgttgt agattggcac gagtatcccc ctcctcaagg tctaagaact
    11041 ttaaataatt tctactgttg tagatggtac catgggaaag aaaggatgag aaaggagaaa
    11101 ggacatctag ataaccggca tatcacggtg gtgtattagt gtagaatagt gaagagaaga
    11161 cttgggaaaa tgtgtaggaa aggttgtttc tgtgtatgtg ggttgggatg ggtggctgtt
    11221 tgagaaggaa cagcgggcag ggcgatgtag tgctgaacgg gcacggaacc actgagactg
    11281 aaggaagtag ggagagagag gggcagggga cgtgcacttt aatctttgcc tcggtagagt
    11341 atacccatgc aagagtatgt ggccacctgt ggtggctttt ggccaggtct ggtgcagtgc
    11401 caatcatctc ccatcaataa tacaacttca gaacaacggc gcattgatgg ggagagagaa
    11461 agtaaattta agtaaggggt acgtagtaga ggattcaact gaaatatttt cgaggagcgg
    11521 ttgggaagtt gaccgattga aaggagaagg gaggggagca ggtgtgatag tcatgtgtaa
    11581 agtaattctt ttttgccgtc gtcacacaat ccacatcaat gataaaatat gtttaaggat
    11641 caatcacacg gagtcggtca taaggcaacc gcaaacgcaa tgcaaactag caagcaagca
    11701 ggaccacaac aacaaccatt accatcacag gcgacagcag cagcagctgc agcagcagca
    11761 aggcaagcaa aggccatctg cgcgtggact tttccaggca ccggctttaa gcgtaatttt
    11821 caatgattgt tgtccgtgta tcctctcacc ctttcaccgc ttgcgcgcac gtgagcagca
    11881 caactggtaa tgcccgagga caatagacga ggaaaaagaa gaagaaaaat gaagagggag
    11941 tgatgaagaa gaagagaaga ataaaagaag acagaaaaga agataaaaaa gtcaaaagac
    12001 gacaaagacg gaaaaaaaag aaacgcgcaa ttttaacacc agcaacgacg acgaaaaggg
    12061 cgtcagcttt gcaccggttt cttagacgga tcgcttgcct gt
//
