LOCUS       sequence-346967-       11648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Tol2 transgenesis of expressed barcode containing SpyCas9, 
            SauCas9, and LbCas12a target sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11648)
  AUTHORS   Takasugi PR, Wang S, Truong KT, Drage EP, Kanishka SN, Higbee MA, 
            Bamidele N, Ojelabi O, Sontheimer EJ, Gagnon JA
  TITLE     Orthogonal CRISPR-Cas tools for genome editing, inhibition, and 
            CRISPR recording in zebrafish embryos.
  JOURNAL   Genetics. 2021 Nov 4. pii: 6420709. doi: 10.1093/genetics/iyab196.
  PUBMED    34735006
REFERENCE   2  (bases 1 to 11648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2021 Nov 4. pii: 6420709. doi: 10.1093/genetics/iyab196."
COMMENT     Sequence Label: pTol2-HybridBarcode
FEATURES             Location/Qualifiers
     source          1..11648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1154..1165,1166..1328)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      4001..4010
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4007..4684
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(4137..4155)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(4187..4207)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4405..4424
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4599..4622
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5669..5688)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5723..5742
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(5814..6527,6528..6530,6531..6533))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5817..6527,6528..6530,6531..6533))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5859..5880)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6208..6227
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6467..6488
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(7832..8019,8020..8031)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8065..8081)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8065..8081)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8118..8138)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8118..8136)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8157..8173)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8157..8173)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8170..8192)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8181..8197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8205..8211,8212..8229,8230..8235))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8250..8271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8388..8405)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8559..9147)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8639..8658)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9318..10109,10110..10178))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9941..9960
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10179..10283)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10309..10764)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10446..10467)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10658..10677
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10891..10913
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10905..10922
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10906..10922
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10932..10950
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcttcctgtt tggatcacta gaaggaggag gtcactcatt acagttctca tatacagtcg
       61 ttggttggtt ggttggttgg tagattgatt gattgattga ttgattgatt gattgattga
      121 ttgattgatt gattgattga ttgattgatt gattgattgg tagtcaaaat aagaaataat
      181 ttccacagat tcattacaga aatgattaaa tgcatacata aaaaactggg gggggggggg
      241 atacaacaac acacttaagt cacatttgcc tacgtaaaga aaagtaaaga aaatcaatag
      301 ctatatttta catctccttt ttttgctgtc ttttaattag ccttgttttg ctgttatctt
      361 gattgaaact gtaacttctt cacctgcttc ttttctttgt aggttttgcc agcccagaaa
      421 caggcatggc tgtgcaaggc cagagcacca tgcacaatgc gctgcatgtc ttcatgaacg
      481 gctcaatgtc ctcagtccag ggctcagcca acgaccccat cttcctcctt caccatgctt
      541 tcattgacag gtaacaaaca cgtcatgaca ttagactgca cagtttttga caaagttcat
      601 acaatctgtt gtttatagct gctacaatta gtgaagtttg tgaatgtact tggatgagca
      661 gcgaaagatc aattgagatc aattgttaga gtttggttgc cctgcagagc aaagaacaaa
      721 aaataatctg gtggctttac tgcgtgaggt tattattggt ggaatagaaa cacaaaacat
      781 aattgcattt atttgtttaa ttttttatct tatcttaact ttcatcttgc atatttgttt
      841 cttacatcat ttctagcatc tttgagcgct ggctaagaac tcatcagcct ccccggtcca
      901 tctacccacg taccaatgca ccaattggcc acaatgacgg ctactacatg gtgccattcc
      961 ttcctcttta taggaatgga gactacctcc tgtccaacaa tgctcttgga tacgagtacg
     1021 cctacctgtt ggacccaggt cattgcacaa caccagaaat gccctctgat ctgcaaaaga
     1081 cgtgaataat ctgttcagac acccatatgc actctgttcc acacaggtca gaggtttgtc
     1141 caggagttct tgacagaggt gtaaaaagta ctcaaaaatt ttactcaagt gaaagtacaa
     1201 gtacttaggg aaaattttac tcaattaaaa gtaaaagtat ctggctagaa tcttacttga
     1261 gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt attaaggaag taaaagtaaa
     1321 agcaagaaag aaaactagag attcttgttt aagcttttaa tctcaaaaaa cattaaatga
     1381 aatgcataca aggttttatc ctgctttaga actgtttgta tttaattatc aaactataag
     1441 acagacaatc taatgccagt acacgctact caaagttgta aaacctcaga tttaacttca
     1501 gtagaagctg attctcaaaa ttgttagtgt caagcctagc tcttttgggg ctgaaaagca
     1561 atcctgcagt gctgaaaagc ctctcacagg cagccgatgc gggaagaggt gtattagtct
     1621 tgatagagag gctgcaaata gcaggaaacg tgagcagaga ctccctggtg tctgaaacac
     1681 aggccagatg ggccattcgt acgcttaatt tcgtataggg ataacagggt aatgatctag
     1741 gtcgacaccg gtgatatcgg gccccctagg atcgatgaat tctcaggggt gtcgcttggt
     1801 tatttccaaa aatcaaatta attttattaa actattagaa cgagcatgtt ttgtctatat
     1861 gctacagaag ataaaaaata ataggagtta acagttataa aacaacacac tttgtttcta
     1921 ttgattgttg accacactgg ggtctcatta agttagatta aagacacact aactgggtca
     1981 aaagcagcag attgatttca tagcaccagg gtaaactttc taacactttt acggcaatca
     2041 tatacattaa aattaaatac agaccacgac tgaacaagga ggatgatctc caatattaaa
     2101 caaagagact tgtgcctatt tctctgaggg taaacatgac ctctcaagtt agcaagttgt
     2161 ttttaacact acaaaaatag ttaagactgc aatcccagaa taaagtattg gttttaacca
     2221 atcaatatag tacagtaaac atccatttgt tttgttgaaa cgttaaacaa atctgaccaa
     2281 agctattagc ttatataaaa caggtttgcc ttctatgtag ctgaaaacac cacaggcccg
     2341 attttgctac tgtgtaaaac atttcagcaa gattttttta ttgcattttt ttttactgaa
     2401 tcgttcaaac attttatcat tttagtttgt tcattcattg caactggaaa aacaacacat
     2461 cacacaaccg cacatatttc agcaataagt acaataaaac actcaaataa aaaaaccatt
     2521 ttaaatctct ttgtattttt gaccgctgtt tcgcgtaatt tcacggtaaa actctggaaa
     2581 tctccactac attcctctca gcggctcctc tcaatgacag ctgaagaagt gacgcggctg
     2641 cctgctgtgt tttgattggt cgaattcact ggaggcttcc agaacagtgt agagtctgaa
     2701 cgggtgcgcg ctctgctgta tttaaagggc gaaagagaga ccgcagagaa actcaaccga
     2761 agagaagcga cttgacaaag aagaaaagag cagcctgaca ggactttttc cccgacgagg
     2821 tgtttattcg ctctatttaa gaatctactg taaggtaagt ctcaatatat tgtactctat
     2881 tggctaatca gaattatata gagattatat gtacttaatg tcaaaaaatc aactttgtat
     2941 atgtaatctt tttacatgtg gactgcctat gttcatctta ttttaggtct actagaaaat
     3001 tatatttccc gttttcacaa taaggatttt taaaaaaagc aatgaacaga cgggcattta
     3061 ctttatgttg ctgacattat tttatatgag cataataacc ataaatacta gcaaatgtcc
     3121 taaatgaatt tgtgttaatg ttgtctacaa aagaaaatta gcgttttact tgtacaacta
     3181 ataataactt ggttattaag agaatttcac ttgttgacta gaaaaatcct ttcataatga
     3241 aacaattgca ccataaattg tataaatata aaattaattc taattgtttt tttttttcct
     3301 gctgcatcga tttgtttgtg agtatgatgt taaatataag caatcgacgt ttgttttgtc
     3361 gtttgttaaa tctagcaatg ttttatttca attaaagtgt taaccggttc atttaaaggt
     3421 gtatttctga atgagctaac gtattagctt ttaacgctag ccggactgtt tggttttgag
     3481 gatcaataaa ccaacgtttt tttctgattt attttgcttt aaaatataaa ggtataattc
     3541 gtcgtcgaga attttgggtt ttgttcgagt ttagaaattt ggcggcgttt ttttgtaccg
     3601 cgaaacaggg cggtggttcg gtgagcttct caattcatct gcttgacaaa cggttttcgg
     3661 caactgtaaa tgcgctatga tgcttttatt cttttcagcg tgtaactttg ttaaatataa
     3721 ttgactttcc gactatcgac gttcgtttca tctccgtgtt attgtttaat ggccattttc
     3781 tgtggtaaac tgtcgccatt ttgttttcag tcgtccgttt gttccgccct tactcacagc
     3841 tgtgtcagca ttagtgatga ctgttctcac ctggcctgtt tgctttcatt attgatgaag
     3901 agctttctag agaattctga agctcgtctt tgtcctgaat tcatctgtat tcatttaaaa
     3961 aaaaaaatta accttaattt ttttttcttc tcagcgtaaa gccaccatgg cctcctccga
     4021 ggacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca
     4081 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     4141 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     4201 ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact acaagaagct
     4261 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     4321 gaccgtgacc caggactcct ccctgcagga cggctgcttc atctacaagg tgaagttcat
     4381 cggcgtgaac ttcccctccg acggccccgt aatgcagaag aagactatgg gctgggagcc
     4441 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc acaaggccct
     4501 gaagctgaag gacggcggcc actacctggt ggagttcaag tccatctaca tggccaagaa
     4561 gcccgtgcag ctgcccggct actactacgt ggactccaag ctggacatca cctcccacaa
     4621 cgaggactac accatcgtgg agcagtacga gcgcgccgag ggccgccacc acctgttcct
     4681 gtagaaatcc ggactcagat ctcgagctcg gatcctttgg aagggaattt tgctacgctg
     4741 tgtcttttgg cttcagaggg ccaaactgca cagagtttgg ctaagcacac caccagccgt
     4801 gatagtttcc agacctccgc tggccatttc cctacatttc aggcagaggt tcctgtcaga
     4861 atatcacgta caaatggaac cgcccgtcgg aatgcggtaa gcggagatac ctaccgatga
     4921 atgcagtaac gcgtctacac cgcatcggaa tcacgagtat gcgtcgctaa cgatccgaat
     4981 gtagtcgacg acgagacgag catcagaata cggagagcat agacatcgag taaggatgga
     5041 ctacagtcgc tacgactagg gtgagacaga gatatcatgc agtatggctc gacagcagta
     5101 tctgctgtca cggcgtgact gcacgacagt cgactaaggg catgcaatta agtctcagcc
     5161 accgttaact gaacatgtca aaacctgtgg agactgttga gatttgatgt tctgaaaaga
     5221 taaagcctat aaataaaatg ttgcccaaat ttcctgcctg atgtttttct ttgtctttgc
     5281 tacatggctt tgctgctcgg atcggctcac tctgtgtatg ccacgttcac tttgtactct
     5341 ccttctcacg gtaggtttat tatttttaga tgtgcagtta gtttctgtga aataacacac
     5401 cacacactga tattgtctgt gcattgactt ggtgagtgca cattgttttt gatcttgaca
     5461 tatttatatt tgattgatca ggtgaactgt gtgaatctaa agtgctccat acagatgttc
     5521 tgcattgaaa atattctcat tttattagtg gaagtgagtg tatgccaccc gggaaagcaa
     5581 gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga tctcttgttt
     5641 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     5701 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     5761 tggatctacg taatacgact cactatagtt ctagaggctc gagaggggcc gctttacttg
     5821 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
     5881 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
     5941 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
     6001 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc
     6061 tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc
     6121 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
     6181 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
     6241 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
     6301 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc
     6361 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
     6421 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
     6481 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtcact
     6541 gtctgctttg ctgttggtct gggctcctgg gtcactggct tactaatgga gtctttatgt
     6601 atgaggactc ttatcaattg ttcttctata aaggtctgca gtgtttctgt tcgtccccta
     6661 catggacacc cagagcctcc taaatacagg agccctgata actgcacaag tgctcagatt
     6721 ccagcagggt ggaaaatgag ataaagtgtg cagatgggga gggggacgtg aatgagagat
     6781 ttgagggatg aaaaggatgg atgaacgcat tgaaaatagc ccctttcaca aaataatacc
     6841 agtaaattgt catataatta atgaccagac ctttacctgt aaatgatgtg atgttcatat
     6901 acagaagaat gccaacaatt tacagatgat tttacaaatt cttaattttc cttttctaaa
     6961 tggatttgtt cacacattat ctcttcaaag caattgaatt ttctggaaaa gattggctgt
     7021 gtgtgtaaaa ggtgctactg aggaatttaa tgtgacatgg aatgaagcaa acagcagcct
     7081 aagacaggat ggcaggaaaa atgtcacatg tttaaaataa gagtgaaacc aacgacctgg
     7141 acttatagag actgtgtgtc cttaggcaaa gctcttgtac aaacagtgct tgtgactgtg
     7201 aaattcgggg tttgcctgga ttgtgttaaa tgtgtgcttg gatgtatcat aaaaaagaat
     7261 cggttctctg tatttgacat tttctgtaat ttatctaaag ttacaactgg ctatgcctga
     7321 tttttatcac tattgaatca atcattttaa agaatgttaa gtctgctgat ttggagagct
     7381 catttacatt tattttgaat gtctcttatt taacacaact gatttaagct ttagatctaa
     7441 tctgatcatt caggcgcgcc aacacgtgaa taccacgcga ggccttaggg ataacagggt
     7501 aatacgcgtg atctgcgaag atacggccac gggtgctctt gatcctgtgg ctgattttgg
     7561 actgtgctgc tcgcagctgc tgatgaatca catacttcct ccattttctt ccactgattg
     7621 actgttataa tttccctaat ttccaggtca aggtgctgtg cattgtggta atagatgtga
     7681 catgacgtca cttccaaagg accaatgaac atgtctgacc aatttcatat aatgtgaaaa
     7741 cgattttcat aggcagaata aataacattt aaattaaact gggcatcagc gcaattcaat
     7801 tggtttggta atagcaaggg aaaatagaat gaagtgatct ccaaaaaata agtacttttt
     7861 gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa
     7921 gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc cccaaaaata
     7981 atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct ggttcttgac
     8041 cccctacctt cagcaagccc agcagatcca ctagttctag agcggccgcc accgcggtgg
     8101 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     8161 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8221 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8281 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8341 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8401 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8461 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8521 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8581 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8641 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8701 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8761 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8821 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8881 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8941 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9001 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9061 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9121 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9181 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9241 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9301 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9361 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9421 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9481 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9541 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9601 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9661 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9721 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9781 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9841 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9901 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9961 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10021 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10081 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10141 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10201 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10261 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
    10321 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
    10381 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
    10441 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
    10501 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
    10561 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
    10621 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
    10681 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
    10741 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
    10801 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    10861 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    10921 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg taccaaatag taggaattac
    10981 ccacctgtac aagtgctgaa aacttggatg aataagcccg tttgccattt tctactgcta
    11041 ttttaaatct tttctgtatt tgtctgcatt tgtctttacc cttgaataaa tgttttagtt
    11101 gttttttttc aattatgact gtgtttaaca gacattatta caataatatg taatagtagt
    11161 acacactatt attatgtaat agtacttgtt gactgtattt gagaactgga ccgagtgagt
    11221 gttacgtcac ccattcaaaa tgacttactt ctggctccaa caaaatgaag ttaattcagt
    11281 tgccattttt cactgtatgg acatcgccgt gttggagcta gacgttgcca ataagcaaga
    11341 aagagccgag atgcgtcgag tctgagtcac cgttcctatg gcaacccctc taaccaatca
    11401 gaagtaagct tgttggaagt ccacagccta ccacttgaaa gcgggctgca caaaatctgt
    11461 caaacgtttt gaacgttgga tgtgagagca catactttta ttaaggcatc tggttggtca
    11521 gtttataata tcaacaactt gggctacaga aaagaaaagt tattacagaa attatgatta
    11581 acaagtacat gttaaataaa gattttaata tgaatgccac cactggagca ttcatgccat
    11641 ttggagct
//
