LOCUS       sequence-346970-        9572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-myristoylation signaling peptide of Src and the C-terminal 
            fragment of G-protein coupled receptor kinase 2 (ct-GRK2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Alten B, Guzikowski NJ, Zurawski Z, Hamm HE, Kavalali ET
  TITLE     Presynaptic mechanisms underlying GABA(B)-receptor-mediated 
            inhibition of spontaneous neurotransmitter release.
  JOURNAL   Cell Rep. 2022 Jan 18;38(3):110255. doi: 
            10.1016/j.celrep.2021.110255.
  PUBMED    35045279
REFERENCE   2  (bases 1 to 9572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-01-18"; volume: "38(3):110255. doi"; pages: " 
            10.1016/j.celrep.2021.110255"
COMMENT     Sequence Label: myristoylated ct-GRK2
FEATURES             Location/Qualifiers
     source          1..9572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        192..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        573..771
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             789..969
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1016..1141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1634..1867
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2052..2096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2394..2511
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2565..3776
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3612..3631
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3807..3848
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     misc_feature    4262..4850
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4315..4335)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4733..4744)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4853..4869)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4854..4870)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5375..5555
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5581..5598)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5587..5811
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5857..6285
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5944..5963)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6154..6175
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6294..6314)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6299..6628
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6479..6614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6541..6560
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6676..6723
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6742..7116
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7246..7367
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7283..7302)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7337..7356
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7416..7432)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7416..7432)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7429..7451)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7440..7456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7464..7470,7471..7488,7489..7494))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7509..7530
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7647..7664)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7818..8406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7898..7917)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8577..9368,9369..9437))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9200..9219
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9438..9542)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
       61 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
      121 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
      181 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      241 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      301 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      361 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      421 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      481 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      541 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      601 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      661 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      721 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
      781 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      841 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      901 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      961 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     1021 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     1081 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     1141 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     1201 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     1261 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1321 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1381 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1441 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1501 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1561 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1621 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1681 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1741 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1801 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1861 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1921 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1981 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     2041 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     2101 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     2161 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     2221 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     2281 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2341 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     2401 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     2461 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2521 tacagggaca gcagagatcc agtttggtta attaagggtg cagcggcctc cgcgccgggt
     2581 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
     2641 gcgcaggagc gttcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
     2701 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
     2761 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
     2821 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
     2881 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
     2941 ggatcgctgt gatcgtcact tggtgagttg cgggctgctg ggctggccgg ggctttcgtg
     3001 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
     3061 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcacaaa atggcggctg
     3121 ttcccgagtc ttgaatggaa gacgcttgta aggcgggctg tgaggtcgtt gaaacaaggt
     3181 ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc
     3241 tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac
     3301 tgactggaga actcgggttt gtcgtctggt tgcgggggcg gcagttatgc ggtgccgttg
     3361 ggcagtgcac ccgtaccttt gggagcgcgc gcctcgtcgt gtcgtgacgt cacccgttct
     3421 gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg
     3481 tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct
     3541 ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct
     3601 tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg
     3661 tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg
     3721 ttagactagt aaatttgtcc gctaaattct ggccgttttt ggcttttttg ttagacgaag
     3781 cttgggctgc aggtcgactc tagaccatgg gttcaagcaa gtccaaaccg aaggatccta
     3841 gccaaagacg tgaattcggc cacgaggagg actacgccct gggcaaggac tgcatcatgc
     3901 atggctacat gtccaagatg ggcaacccct tcctgaccca gtggcagcgg cggtacttct
     3961 acctgttccc taaccggctc gagtggcggg gcgagggcga ggccccgcag agcctgctga
     4021 ccatggagga gatccagtcg gtggaggaga cgcagatcaa ggagcgaaag tgcctcctcc
     4081 tcaagatccg aggtggcaag cagtttgtcc tgcagtgcga tagtgaccca gagctggtgc
     4141 agtggaagaa ggagcttcga gacgcctacc gcgaggccca gcagctagtg cagcgggtgc
     4201 ccaagatgaa gaacaagcca cgctcacctt aagttaacaa ttcgatatca agcttatcga
     4261 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     4321 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     4381 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     4441 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     4501 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     4561 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     4621 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     4681 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     4741 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     4801 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     4861 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     4921 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     4981 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5041 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     5101 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     5161 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     5221 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     5281 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     5341 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     5401 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5461 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5521 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     5581 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     5641 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     5701 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5761 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     5821 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     5881 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5941 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6001 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6061 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6121 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6181 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6241 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     6301 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6361 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6421 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6481 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6541 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     6601 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     6661 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     6721 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     6781 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     6841 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     6901 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     6961 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     7021 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     7081 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     7141 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     7201 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     7261 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7321 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     7381 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7441 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7501 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7561 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7621 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7681 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7741 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7801 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7861 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7921 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7981 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8041 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8101 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8161 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8221 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8281 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8341 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8401 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8461 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8521 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8581 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8641 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8701 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8761 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8821 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8881 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8941 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9001 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9061 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9121 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9181 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9241 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9301 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9361 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9421 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9481 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9541 cgcacatttc cccgaaaagt gccacctgac ac
//
