LOCUS       sequence-346984-        9279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for HA-tagged rat Neurexin 1alpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Taniguchi H, Gollan L, Scholl FG, Mahadomrongkul V, Dobler E, 
            Limthong N, Peck M, Aoki C, Scheiffele P
  TITLE     Silencing of neuroligin function by postsynaptic neurexins.
  JOURNAL   J Neurosci. 2007 Mar 14;27(11):2815-24.
  PUBMED    17360903
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2007-03-14"; volume: "27(11)"; pages: "2815-24"
COMMENT     Sequence Label: pCAG-HA-rat Nrxn1alpha
FEATURES             Location/Qualifiers
     source          1..9279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          135..1151
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1075..1097
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1159..1178
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1396..1422
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1429..1440)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(5794..5813)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5859..5914
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5913..5932)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6275..6291)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6275..6291)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6288..6310)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6299..6315
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6323..6329,6330..6347,6348..6353))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6368..6389
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6448..6643
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6494..6629
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6649..6783
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6686..6705)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6740..6759
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6851..6868)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7022..7610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7102..7121)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7781..8572,8573..8641))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8404..8423
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8642..8746)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8777..9156
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(9158..9279,1..134)
                     /label=chicken beta-actin promoter
                     /note="contains a 21-bp deletion relative to the standard 
                     chicken beta-actin promoter"
ORIGIN
        1 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg
       61 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
      121 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
      181 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
      241 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
      301 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
      361 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
      421 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
      481 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
      541 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
      601 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
      661 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
      721 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc
      781 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
      841 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
      901 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
      961 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     1021 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     1081 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
     1141 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     1201 aattcgcggc cgctagcgct acgggactca gatctcgagc tcaagcttgg gaccatggca
     1261 cttcccagat gcatgtggcc aaattatgtt tggagagcta tgatggcatg tgtggtccac
     1321 aggggatccg gtgccccatt gactctctgc ttgttgggat gtttgctaca gacttttcac
     1381 gtactctctc aaaagtatcc atacgacgtt ccggactacg caactagtct ggagttccca
     1441 ggcgctgagg gccagtggac gcgcttcccc aagtggaacg cgtgctgcga gagtgagatg
     1501 agcttccagc tgaagacacg cagcgcccgc ggcctcgtgc tctacttcga cgacgagggc
     1561 ttctgcgact tcctcgagct catcctgaca cgcggcggcc gcttgcagct cagcttctcc
     1621 atcttctgcg ccgagcccgc cacgctgttg gccgacacgc cagtcaacga cggcgcctgg
     1681 cacagcgtgc gcatccgccg ccagttccgc aacaccactc tctacatcga ccgcgctgag
     1741 gccaagtggg tggaggtcaa gtccaagcgc agggacatga cggtgttcag tgggctgttc
     1801 gtgggcggcc tgccgcctga actgcgagcc gctgcgctca agctcaccct ggcctcggtg
     1861 cgtgagcggg agcccttcaa gggctggatc cgtgatgtca gggtcaactc gtcccaggcg
     1921 ctgccggtgg acggcagcga ggtcaagctg gacgaagagc cgcccaatag cgggggcggg
     1981 agcccgtgcg aggcgggcga tgagggcgac ggcggcgtgt gcctcaacgg gggcgtgtgc
     2041 tccgtggtcg acgaccaggc cgtgtgtgac tgctcgagga ccggtttccg tggcaaagac
     2101 tgcagccaag gaaaagaaga gtatattgcc acattcaagg gatctgagta cttctgctac
     2161 gacttatccc aaaaccccat tcaaagcagc agcgacgaaa taactctgtc ctttaaaact
     2221 cttcagagga atggactgat gcttcacaca gggaaatcgg ctgattatgt caaccttgcc
     2281 ttgaaaaatg gagctgtctc tctggtcatt aatttgggat caggggcctt tgaagcgcta
     2341 gtggagcccg tgaatggaaa gtttaatgat aatgcctggc atgatgtgaa agtcaccagg
     2401 aatctgcgtc aggtgacaat atcagtggat gggattctta ccacaacggg ctacacgcaa
     2461 gaagattaca ccatgctggg gtctgatgac tttttctatg ttggaggcag ccccagcaca
     2521 gccgaccttc cagggtcacc agtcagtaac aactttatgg gctgtctcaa agaggtcgta
     2581 tataaaaata atgatgtaag actggagtta tcgcgacttg ccaagcaagg agatcctaag
     2641 atgaagatcc atggtgtggt ggcttttaag tgtgaaaatg tggcaacctt agacccaatc
     2701 acttttgaga ccccggaatc tttcatttct ttgcctaagt ggaatgcaaa gaaaacaggc
     2761 tcaatatcat ttgatttccg tacaacggag ccaaatggcc tcatcttgtt tagccatggc
     2821 aagccaagac atcagaagga tgccaagcac ccgcagatga ttaaggttga cttttttgct
     2881 attgaaatgc tggatggcca cttgtacctc ctcttagaca tgggatcagg tactataaaa
     2941 atcaaagccc ttcagaagaa agtcaatgat ggagaatggt atcacgtgga tttccagcga
     3001 gatggacggt caggtaccat ttctgtcaac acacttcgta ctccctacac agctcccggt
     3061 gagagtgaga tcctagacct cgatgatgag ttgtatctgg gtggcttgcc agaaaataag
     3121 gctggcctgg tcttccccac tgaggtgtgg actgctctgc tcaactatgg ctacgtaggc
     3181 tgcatccggg atctattcat tgatggccag agcaaagata tccggcagat ggcggaaatt
     3241 cagagtactg ctggagtgaa gccatcctgc tcaagggaga ctgcaaaacc atgccttagc
     3301 aatccttgca aaaacaatgg catgtgcagg gatggctgga acagatacgt ctgtgattgc
     3361 tctggaacag gctatctcgg caggtcctgt gagagagagg caacggtttt gagctacgat
     3421 gggagtatgt ttatgaaaat tcagctccct gtggtgatgc acactgaggc cgaggacgtt
     3481 tccttacggt tcagatccca gcgtgcgtat ggcattctga tggcgaccac ttctagagac
     3541 tccgcagata cccttcgact ggagctagat gcagggcgcg tgaaactcac ggtcaatcta
     3601 gattgtatca ggattaactg taattccagc aaaggtcctg agaccctttt tgccggctat
     3661 aacctcaatg ataatgaatg gcacacagta cgtgtggttc ggcgaggaaa aagtttaaag
     3721 ctaaccgtgg atgatcaaca agccatgaca ggccaaatgg caggtgatca tacaaggctg
     3781 gagtttcata acatagagac aggcatcatc acagaacgac gctatctttc ttctgtcccc
     3841 tccaacttca ttgggcacct gcaaagcctc acatttaatg gaatggcata cattgacttg
     3901 tgcaaaaatg gtgatataga ttattgtgaa ctcaatgcca ggtttggatt caggaacatc
     3961 atcgcggatc ctgtcacctt caagaccaaa tcaagctatg ttgccttagc tacattgcaa
     4021 gcctacactt ctatgcatct atttttccaa ttcaagacaa catccctaga tggactaatt
     4081 ctgtataaca gtggggatgg aaatgacttt attgtggttg aattagttaa agggtactta
     4141 cattatgtgt ttgacttggg aaatggtgct aacctcatca aagggagctc aaacaaacca
     4201 ctcaacgaca atcagtggca caatgtgatg atttcaaggg acaccagcaa tctccacaca
     4261 gtaaagatcg acacaaaaat cacaacacaa atcactgcgg gagccagaaa cttagacctc
     4321 aagagtgact tgtatattgg aggagtggct aaagaaacat acaaatcgtt gccgaaactg
     4381 gtccatgcta aagaaggctt tcaaggttgc ctggcatctg tggacttgaa tggcaggctt
     4441 ccagacctca tatctgatgc tcttttctgc aatgggcaaa ttgagagagg atgcgaaggg
     4501 cccagcacaa cctgtcaaga ggactcatgt tcaaatcaag gcgtgtgctt gcagcaatgg
     4561 gatggcttta gctgtgactg tagcatgact tccttcagtg gcccgctctg caatgaccct
     4621 gggacgacgt atatctttag caaaggtggt ggacaaatta catataagtg gcctcctaat
     4681 gaccgaccca gtacacgagc agacaggctg gccatcgggt ttagcactgt tcagaaggaa
     4741 gcagtgttgg tgcgtgtgga cagttcctca ggcctgggtg actaccttga gctgcacata
     4801 caccaaggaa aaattggagt taaatttaat gttgggacag atgacatcgc cattgaggag
     4861 tccaatgcaa tcattaatga tggaaaatac catgtagtgc gtttcacgag gagtggtggc
     4921 aatgccacgt tacaggtgga cagctggcca gtcatcgaac gataccctgc agggcgtcag
     4981 ctcacaatct tcaatagcca agcaaccata ataattggcg ggaaagagca gggccagccc
     5041 ttccagggcc agctctctgg gctttactac aatggcttga aagttctgaa tatggcggca
     5101 gagaacgatg ccaacatcgc catagtggga aatgtgaggc tggtcggtga agtgccttcc
     5161 tctatgacaa ctgagtcgac agccactgcc atgcagtccg agatgtccac ctcaatcatg
     5221 gagaccacca caaccctggc taccagcaca gctcgacgag gaaagccccc cacaaaggag
     5281 cctatcagcc agaccacgga tgatatcctt gtggcctcgg cagagtgtcc cagcgacgat
     5341 gaggacattg acccctgtga gccgagctca ggtgggttag ccaaccccac ccgagtgggc
     5401 ggccgcgaac catacccagg ctcggcagag gtgatccggg agtctagcag taccactggc
     5461 atggtggtgg ggattgtcgc agcagcagct ctgtgcatcc tcatcctcct ctatgccatg
     5521 tacaagtaca gaaaccggga tgaaggctca tatcacgtgg atgagagtcg aaactacatc
     5581 agtaactcag cacagtccaa tggggctgtg gtcaaggaga agcagcccag cagtgcgaaa
     5641 agcgccaaca aaaacaagaa gaacaaggat aaggagtatt atgtctgact cgaggtaccc
     5701 ccggggagct caattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
     5761 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
     5821 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     5881 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     5941 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     6001 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     6061 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     6121 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     6181 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     6241 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6301 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6361 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6421 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc
     6481 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6541 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6601 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt
     6661 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6721 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6781 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6841 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6901 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6961 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7141 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7201 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7261 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7321 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7381 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7441 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7501 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7561 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7621 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7681 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7741 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7801 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7861 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7921 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7981 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8041 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8101 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8161 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8221 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8281 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8341 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8401 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8461 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8521 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8581 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8641 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8701 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8761 aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc aattacgggg
     8821 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     8881 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     8941 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     9001 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     9061 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     9121 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     9181 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     9241 ttattttgtg cagcgatggg ggcggggggg ggggggggg
//
