LOCUS       sequence-347017-       12003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the humanized fnCas12a gene sequence
            tagged with Nlux and type IIS restriction site for endonuclease BaeI
            at the crRNA region that facilitates the generation of mulitpl.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 12003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_hfnCas12a_CRATES
FEATURES             Location/Qualifiers
     source          1..12003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    164..667
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        704..808
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(809..877,878..1669)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1027..1046)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1840..2428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2329..2348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3388..3762
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      4647..4656
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5337..5357)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5362..5371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5373..5399)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(9837..9857)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(10078..10089)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aaaatgaaga gggagtgatg aagaagaaga gaagaataaa agaagacaga aaagaagata
       61 aaaaagtcaa aagacgacaa agacggaaaa aaaagaaacg cgcaatttta acaccagcaa
      121 cgacgacgaa aagggcgtca gctttgcacc ggtttcttag acggatcgct tgcctgtaac
      181 ttacacgcgc ctcgtatctt ttaatgatgg aataatttgg gaatttactc tgtgtttatt
      241 tatttttatg ttttgtattt ggattttaga aagtaaataa agaaggtaga agagttacgg
      301 aatgaagaaa aaaaaataaa caaaggttta aaaaatttca acaaaaagcg tactttacat
      361 atatatttat tagacaagaa aagcagatta aatagatata cattcgatta acgataagta
      421 aaatgtaaaa tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc
      481 ggcattaata cctgagagca ggaagtacaa gataaaaggt agtatttgtt ggcgatcccc
      541 ctagagtctt ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac
      601 tttctatttt taatttatat atttatatta aaaaatttaa attataatta tttttatagc
      661 acgtgatgaa aaggacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      721 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      781 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      841 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      901 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      961 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1021 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     1081 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1141 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1201 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1261 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1321 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1381 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1441 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1501 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1561 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1621 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1681 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1741 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1801 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1861 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1921 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1981 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2041 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2101 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2161 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2221 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2281 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2341 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2401 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2461 ggccttttgc tggccttttg ctcacatgtt taaacggggt cgtttatttc tctgccagcc
     2521 ttggacctcg tggttgggcg tttcctcctc cttggtgcac tgaacctgtc cgcatcctgc
     2581 gtcggtttgg cggtcttttg tcctttcctc tatagcccgc ccgtctagag ggcacacgcg
     2641 atgatcttta tatctcttca tgtgtctttg ttttaactag gatactgccg ggtgaatgcc
     2701 catcagacaa gaggccaaac tctatctaca cccttttgac ttctgttgtg gtcgtagtgt
     2761 gtgcttgcat gccctgaaag tccaggcatc ccacttgtgc tctaacccca ttcaaaacag
     2821 cagaagtgct taattaagat atagattcat gatctcctgt cccctccttc ttaccttttc
     2881 acaaacctca cacagaagtc tccactcttc gcctctaaaa cctcttttta aattatggta
     2941 agttcgtgcg gcagtgggtt ttcggatcta tatttgtcaa gatccagttc aaggtcaggg
     3001 atgtagatta agtacagaag gagaagcaca agcgcgccag ttcgcccctc acggcctgga
     3061 gcagggcatt taatccctct atcttaccag aaccatacta tacaaccaat cctgttggca
     3121 tcgctctgtc tatttgtcgt gcgtgcatgt gtccatggtg tggtgggggg caggggtttt
     3181 cggggttgcg gttgaaggca ccttatcaga aagatgccct cagagataga ggtagccccc
     3241 tccccccgat cttcgaccag tcctgtcagg cgaacacttt cacccgtcgt tcacctcgtt
     3301 acacacaagg agtagacctc tgaagttcta attgtcataa atgcccctcc cccctccctc
     3361 cttcccttca tcctcccctc cgagcagatg gccaagttga ccagtgccgt tccggtgctc
     3421 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     3481 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     3541 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     3601 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     3661 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     3721 gccggcaact gcgtgcactt cgtggccgag gagcaggact gatggccagg gatcaggagg
     3781 agggagtgaa gaggagaagg gatctggttt cagagatccc cacttctgcc gtcgtctttc
     3841 ggccttcctt ccttttaggt gtcatgcctt aggtccttca agtcctcacc tgtcgtcgtc
     3901 atgtgtgtgt gtgcccgtca tacaagtcac tcgatccaat tcacgcatcg gttcaatcaa
     3961 aataagacta gaccccgagg gaagaagggc agaaggaaat cgaaggggtg ggatgtgtgt
     4021 gagagaggga aggagaaatg aaagaagtga acaatgtcat ggtagccagt aaggagagag
     4081 tagaagcgaa gaaagcaaaa gcactgttgt gaagaaacga aatggaagat ggtcatcgct
     4141 cctggctcta cttgtggttt ttctatcttt aatttcaggc gtcctggtct cgttacatca
     4201 gctcccttat ctcattggtt tatcccctac tctactgctg cttcttcctt ccatccgtga
     4261 ctgtataaca acgaattgta gtaccgcaga tagacaatag aaaaatgcca aaaaaggcat
     4321 cattgatttg ctcctcccca ttaagtcact gtacgccacc atcgccacta ccctccctta
     4381 gcaaatagtc tttatttaga acacagaaga agagtacgtt gtgtgcatgc gtgttgttcg
     4441 tacaacttcc gtgtcaggac gagagaccat ttcaacagtg agtaattccg cctgcgttta
     4501 ttttttcctc tttttccttt tcttgttttc ttcctttgta cgtgtatttc cctctcttcc
     4561 acgtcttctt tatcgtcatc ttgtctgcca tccttactct atcttctacc ctcactcact
     4621 tccacactct caagtccacc ctcgcggccg ccatggcctc tagtggatca gcttgcatgc
     4681 ctgcaggaga cgatatcacc tcttctgttt ccacgataaa aatagactgc tcatttcttc
     4741 gtcgtcttca tcgtctgctt tttctgcttc gcctctgtct ggggtctgaa accactacac
     4801 acacacacaa cactcgtact cccactttca caaaagcgta agctcaccgg cttttcttac
     4861 acgtacattt tagtggatcc catcacgcca ctaccacgcc cgcgggggat ggaacggagg
     4921 ggagagagag aagggggaag catggatgaa tgagacattg agggaaagga ggggagggag
     4981 cagtccatca gggcgctacc tctcttgtcc cccaaaccct gttgagccgt tcaacatgtt
     5041 tcatgtttcc tccctccccc cttccctccc tggcctttcc gcggagccat tcaagtgacg
     5101 tctggaccgc accgtaacaa aatcgtttct atggggggtt tgtttgacaa ccacgtcttc
     5161 agcgttttta aaaaaaaaag cgggcaagcc ctctcaccct cactcatgcc catcctcctc
     5221 ctctcctgcg gaacattctt acaaaaggcg taactcgacg acaactcaaa gaacgacaaa
     5281 catcaatccc aaaaaaaaaa tctctactcg tctctcttgg atctttccaa ttgtcagacc
     5341 ttcctcttct ttttcgggct agccaccatg gacgcgtagt cgggcacgtc gtaggggtat
     5401 ccggccagga tgcgctcgca caggcgccag ccggttaccc cgttgatggt cacgcggaac
     5461 aacagcgagc catccgggtt gatcagccgc tcgtcaatga tcttattgcc gttccacagg
     5521 gtgccggtca ccgtgatctt cttgccgtcg aacacggcaa tgccctcgta aggacggccg
     5581 aagtagtcaa tcatgttcgg cgtcacaccg tcgatgacca gcgtcccgta gtgcagtatc
     5641 accttgaagt ggtgatcgtc cacggggtac acaaccttga agatcttctc gatctggccc
     5701 atttggtccc cgctcaggcc ctcgtaggga atgatgacgt ggatgtcgat cttcaggccg
     5761 ttctcgccgc tcaggacgat gcgctggatt ggggtcacgg agacgcccag gttctggaaa
     5821 agcgaggaca caccgccctg ctccagcacc tggtcgaggt tatagccagc tgtctgccgc
     5881 cagtcgccca cgaagtcctc gagagtaaac accatgttaa cggacccgct gccggacccg
     5941 ttatttctat tctggacaaa ctcgaagtat tcctcgttct taatgaccag gttcagcttc
     6001 ttcccctcct gattgttctt gatccggccc agcagcatca gtcccttcag cccgatgtgg
     6061 taggccccgt tggcgtctgc atcctgaggc atatttttgg gtgcctgtct gctgtcgaag
     6121 aagtttccgt tgacatcagc cacggggcta atcagatagt ccagttctgt cccggtcttt
     6181 gagttccgca tctgcaggat tgtattcagc actgaggtca gcttggcgaa aaatttctta
     6241 tcactctcgc cacagatggc tgccttgatg cattcgccat gtccatactc gatagagtag
     6301 tctttcagca gcttttccag ctccttggtt gggtacacct ccctagtgtc ccaattgtgg
     6361 tttttatcgg aatttcgaaa gttgatcagg cgagatccga aggaggcaat ggtccatttc
     6421 cccttagcgg ccttgtcgcc gaagttcttg taatcgaagg aaaactcgaa gtaccccttg
     6481 tccagattat aacagatctt atcgaacttg ctgaaaaatt cctgggactt gctcactgac
     6541 tcatatttag ggtacagctg gttgacaaag ccggtcacgg ggcagatctt ggaagtgaat
     6601 ccggctggca catagtagat gatgcctgtc tgttttccca ttttcttgaa agtttcaaag
     6661 ggagctgtca gctggtaggc tctcaggacg cctccggtct tgtcgaactc gttatcttta
     6721 aacaccaggt aattcagctt ttcgatcagc attttctcca gcttctgata gacctgtttt
     6781 tccaccttaa agcgccccct cttaaagccg aagttcagat cctcgaacac cacaatggca
     6841 ttgtattcga tgaccagctt tgcaatctca tggaccacct ggctcagata gccttccttc
     6901 atctccttaa tgttgttgat tttcttccag tccttcctag ctgagtctct atccttctcg
     6961 attgcagcca gtttatcgtg gtagttggtt ttcatccggt cattgccaat gatgttgaat
     7021 gtatcctgct taatgatatt ccctttgcca tccaccaggg tatagtaggc cagatgccgc
     7081 tctcctcggt caatgctcag gatgtgcaca tcgtttgctt tttccttcag cagcagattg
     7141 atctcgtcgt tgaacttgtt agcgccgctt gacttgaagt taatggtgat tggacaatgg
     7201 aaaaagaact tatcttcggt gaaccgcttg tccttaatca gatcgtattc gaacacgctc
     7261 tctttcttag gattgtcctt gttcttgtta gcgatggcct ccttagctgg gtgagtgatt
     7321 ttcttaggaa tagactgctt ccggtaaaac agttcggcct ctccgttcag tttatagacc
     7381 acgtcctgca gattcctctc atcgaacagg gccttccagt acagtgtatg caggtttggt
     7441 ctgcccttgc tgtatgctga aaaatctttg ttatagatct ggaacaggta cagctttccc
     7501 tgattgacca cgctgtcgat gtaactttca gaaatgttct caaaagtcag cttatacccc
     7561 tgattttcca cctcgcggta gaactcgtcg atagagttat atctctgtgt gtcggaaaac
     7621 cggaagccaa aatccttcca ttcagggtgt ttggagatgc tctgcttgta gaagtcaata
     7681 aacttcctgc aatcctcgat gttgaactca aatttctcat atcccttctg ggggctcccg
     7741 ttcttagtat gtgttgagtg atttctaatc cgcaggatgt cctcggatgg gttgtagaat
     7801 ttgatactct tggcgctgaa gaacacctta ggcagcatct tatttgcgcc gggcagcagc
     7861 ttatacacga ttttcttgta tccttcccct ttgttctcct taatggcttt gtcatcgaag
     7921 atcttattgt ttttcttatt catcactccc agatagtact tgtcatcctt gatgaacagg
     7981 atagctgtgt tgtcaggctc cttgttctta tcccacccgt tggccagggt actattttcg
     8041 aagttcagtt taaacttctc gtcagagtaa ggcttctgtg tgatgtagtt cctaatcttg
     8101 ttatacaggg ggacaatgtt tgccagctcg aagtaacatt cctcgaacac caggtagaag
     8161 tgttcgtctt tatccaggat attagcctta tcctctgact gactaatatg gaagattttc
     8221 agcttgtgca gcagattgtt ggtctggtcc agcagatcct tgatggcttt cacgtcatct
     8281 tctgctgaag cctgcagcag gtctttcttg ccctggttct ggtacttaat actgatctga
     8341 gccagattgt ctttgttctg ggcgatctca tcaaaaatca tggggatggc tgcgaagttg
     8401 gccaggattt cctcaaagcg gcactgtttg tcaatatccc tgtgcttgtt gaactcctcc
     8461 agtgccagct taattgtttc cagactcaga tattttgcct tctcggtttt cttagcgatc
     8521 agttcctgct ctttcttaga gggattatcc aggttctttg gagcgatctg ctgtgtaatg
     8581 tactccagga cggcggtccc aatcacggaa tagtcatcaa acacctgctg tgacaggtct
     8641 gtcagactct tatcgttttt gaagtagatc ttactcaggt ccagcttctg ggctttcagg
     8701 tcatcgaaca gcagactcag agtttcctta atagattttt cctccactgt cttgaaagcg
     8761 gcgatctgct cataaaagct ctgcatggta gtgaccacgt ctgagtcatc ttccagttta
     8821 tcaatgacaa aagacttgga ctcggtatca ctcaggatct gtttgaacag cacagacatc
     8881 ttgtatttct tcagagtttt gtcgttgatc tgctggctat acagattgat gtattcgtta
     8941 attcccttgc gcttggtatt ctccccgttc acaaacttcc cgccaatgat ggtattgaat
     9001 ttagtaatgc cggactggtt caggtaattg ttgaaattgg cgatttcaaa cacctcgtcc
     9061 agggaaaaga ccctctggtt cacctcgcta gtcttatagt cgatatcgaa tgtcagttcc
     9121 tcagccagat ctttcttgat ctgttcgtaa ttaattgcct cgggagcttt gtccttcaga
     9181 gattcatatt tggccttgtt ctccaggaac tttggcagat tgtcatcgac gattcggtag
     9241 atgatggagg taggaatgtc gttgctggag tacacgttct tgcggttctc gtggaagcct
     9301 ttaaagtatg tggtccatcc cttgaagctc ttgatgattt ccagtgcctc gtcaatatca
     9361 gtgatatcgg agttagcctt gaacagttca atcccattgt ccttagactg tttcagccac
     9421 aggatcaggt cggactcctg gcctttctta gcatcgatca gattctggtt gaacagattt
     9481 ttaaactttt cggagtcctt gatgtactcg ctaatctgtt tcttgatggt gtccttggcg
     9541 gacttgaagt ccttctgcag gttgtcatcg tcgctcttct tcagcttgaa gtacacgtct
     9601 gagtaattct gcagcagatc ctcactgatg cacacggagc tcagaatttc ctcgataaag
     9661 aactggtggt atttatcaat gatctgcttg gctttcttat agtcttttgc ccgcttctca
     9721 tcgtccagaa tcaggcctcg ggccttgatg ttctccagtg tcttgccctg tgggatcagc
     9781 tcgaaccgca gtgtcttact cagtgaatac ttgttgacga actcctggta gatgctcacc
     9841 ttccgctttt tcttgggcat gctcgagggt tgcgtgtgta tctgtgtgca gtggatattg
     9901 ttaccgagtt tggtgagcgt gagtccgtta gtgccctggt ggtggtggat taggagagtg
     9961 ggtgactcgg tgtccatggc tttcttcgct cattatagga ggggaaagga atgagggagg
    10021 gtggggagac cgcgtctgtt gttgaccacc gatttacttc ttgcctccct tcccctccct
    10081 cccctcaatc cgtacgacac aaatagtagc cgagtgtctg ctgcagagcg catgattagt
    10141 gtggtagaca acgagggagg gaaggatgta cagggcatgg cacggagaag cgatggtggc
    10201 caggaagagg agaggtcgcg agaacaggat gtgttgcgaa tggataaaaa cagaaagcga
    10261 tggctctggg cttcgaaagc aggggacatt aggacgtgta gaccatctcg acggatccct
    10321 ctgtatctct gttgtgcgtg aatgttttct gtgcacgtgt agtgtgtgag agtagaaccc
    10381 gggaactcga acagagaaaa gcatgggtgg ctgtggtgtg gaggcttcgt tcccaccaca
    10441 tgcccttctc cttcgcctcg cctctccctg ccttcttcca cgcacccttg cgcccctcgt
    10501 tctcaatacc tggctcactt ccaccattca aacaaccatc acgatacagg catttatcta
    10561 tcgttgaaga cttcttcctc cggtagatct tagccaaggt aagaagaggg gcatgcagca
    10621 aggagaaaga aatgatgcat gatgaggaat agaaggggag gagggaggga tatgatggga
    10681 agcgaaagcg catattctgg tggtctgctg cctgatgggg acgcgtctag ctgtgacact
    10741 gaggacggtg gctgctggtg gctgcgggcg ctgccttggt gatcaatggg agtaaaggga
    10801 gggaaggagg tagcgtgaac ggatgacgcg gagaagttta ggggtctctt tacgtatcgc
    10861 ccctgccgcc cgcctctctg cgataaatgt gcctgttacc ctgcagcctc tattcttcac
    10921 tgtgttcctg ttttccaaca gcctctattc ttccctgtct tttgttgcag tggcgtcatc
    10981 ctctctttgc cccagtcgtc gttctctcga ctcactcact ccccccctcc ttccctccct
    11041 ccatccacag aatcgatgaa gagcaggcct tggctcttcg gatcccatga cgctagtatc
    11101 cagctgggtc taagaacttt aaataatttc tactgttgta gatggtacca tgggaaagaa
    11161 aggatgagaa aggagaaagg acatctagat aaccggcata tcacggtggt gtattagtgt
    11221 agaatagtga agagaagact tgggaaaatg tgtaggaaag gttgtttctg tgtatgtggg
    11281 ttgggatggg tggctgtttg agaaggaaca gcgggcaggg cgatgtagtg ctgaacgggc
    11341 acggaaccac tgagactgaa ggaagtaggg agagagaggg gcaggggacg tgcactttaa
    11401 tctttgcctc ggtagagtat acccatgcaa gagtatgtgg ccacctgtgg tggcttttgg
    11461 ccaggtctgg tgcagtgcca atcatctccc atcaataata caacttcaga acaacggcgc
    11521 attgatgggg agagagaaag taaatttaag taaggggtac gtagtagagg attcaactga
    11581 aatattttcg aggagcggtt gggaagttga ccgattgaaa ggagaaggga ggggagcagg
    11641 tgtgatagtc atgtgtaaag taattctttt ttgccgtcgt cacacaatcc acatcaatga
    11701 taaaatatgt ttaaggatca atcacacgga gtcggtcata aggcaaccgc aaacgcaatg
    11761 caaactagca agcaagcagg accacaacaa caaccattac catcacaggc gacagcagca
    11821 gcagctgcag cagcagcaag gcaagcaaag gccatctgcg cgtggacttt tccaggcacc
    11881 ggctttaagc gtaattttca atgattgttg tccgtgtatc ctctcaccct ttcaccgctt
    11941 gcgcgcacgt gagcagcaca actggtaatg cccgaggaca atagacgagg aaaaagaaga
    12001 aga
//
