LOCUS       sequence-347028-       12163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the humanized fnCas12a gene sequence
            tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the
            Nitrate reductase gene and one spacer targeting the LPAAT gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 12163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_hfnCas12a_NR1_NR2_LPT1
FEATURES             Location/Qualifiers
     source          1..12163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2708..2719
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2940..2960
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7398..7424
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      7426..7435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7440..7460
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8141..8150
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(9035..9409)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      complement(10369..10957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10449..10468)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11128..11919,11920..11988))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11751..11770
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11989..12093)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(12130..12163,1..470)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 ttttaatata aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata
       61 gtttttgttt tccgaagatg taaaagactc tagggggatc gccaacaaat actacctttt
      121 atcttgtact tcctgctctc aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa
      181 gaccacacac gaaaatcctg tgattttaca ttttacttat cgttaatcga atgtatatct
      241 atttaatctg cttttcttgt ctaataaata tatatgtaaa gtacgctttt tgttgaaatt
      301 ttttaaacct ttgtttattt ttttttcttc attccgtaac tcttctacct tctttattta
      361 ctttctaaaa tccaaataca aaacataaaa ataaataaac acagagtaaa ttcccaaatt
      421 attccatcat taaaagatac gaggcgcgtg taagttacag gcaagcgatc cgtctaagaa
      481 accggtgcaa agctgacgcc cttttcgtcg tcgttgctgg tgttaaaatt gcgcgtttct
      541 tttttttccg tctttgtcgt cttttgactt ttttatcttc ttttctgtct tcttttattc
      601 ttctcttctt cttcatcact ccctcttcat ttttcttctt ctttttcctc gtctattgtc
      661 ctcgggcatt accagttgtg ctgctcacgt gcgcgcaagc ggtgaaaggg tgagaggata
      721 cacggacaac aatcattgaa aattacgctt aaagccggtg cctggaaaag tccacgcgca
      781 gatggccttt gcttgccttg ctgctgctgc agctgctgct gctgtcgcct gtgatggtaa
      841 tggttgttgt tgtggtcctg cttgcttgct agtttgcatt gcgtttgcgg ttgccttatg
      901 accgactccg tgtgattgat ccttaaacat attttatcat tgatgtggat tgtgtgacga
      961 cggcaaaaaa gaattacttt acacatgact atcacacctg ctcccctccc ttctcctttc
     1021 aatcggtcaa cttcccaacc gctcctcgaa aatatttcag ttgaatcctc tactacgtac
     1081 cccttactta aatttacttt ctctctcccc atcaatgcgc cgttgttctg aagttgtatt
     1141 attgatggga gatgattggc actgcaccag acctggccaa aagccaccac aggtggccac
     1201 atactcttgc atgggtatac tctaccgagg caaagattaa agtgcacgtc ccctgcccct
     1261 ctctctccct acttccttca gtctcagtgg ttccgtgccc gttcagcact acatcgccct
     1321 gcccgctgtt ccttctcaaa cagccaccca tcccaaccca catacacaga aacaaccttt
     1381 cctacacatt ttcccaagtc ttctcttcac tattctacac taatacacca ccgtgatatg
     1441 ccggttatct agatgtcctt tctcctttct catcctttct ttcccatggt accatctaca
     1501 acagtagaaa ttatttaaag ttcttagact acttcctcgc ggtactcatt ctccatctac
     1561 aacagtagaa attatttaaa gttcttagac cttgaggagg gggatactcg tgccaatcta
     1621 caacagtaga aattatttaa agttcttaga ccagcacagg acgcgattgt cgtggaagtg
     1681 gatctacaac agtagaaatt atttaaagtt cttagacgaa gagccaaggc ctgctcttca
     1741 tcgattctgt ggatggaggg agggaaggag ggggggagtg agtgagtcga gagaacgacg
     1801 actggggcaa agagaggatg acgccactgc aacaaaagac agggaagaat agaggctgtt
     1861 ggaaaacagg aacacagtga agaatagagg ctgcagggta acaggcacat ttatcgcaga
     1921 gaggcgggcg gcaggggcga tacgtaaaga gacccctaaa cttctccgcg tcatccgttc
     1981 acgctacctc cttccctccc tttactccca ttgatcacca aggcagcgcc cgcagccacc
     2041 agcagccacc gtcctcagtg tcacagctag acgcgtcccc atcaggcagc agaccaccag
     2101 aatatgcgct ttcgcttccc atcatatccc tccctcctcc ccttctattc ctcatcatgc
     2161 atcatttctt tctccttgct gcatgcccct cttcttacct tggctaagat ctaccggagg
     2221 aagaagtctt caacgataga taaatgcctg tatcgtgatg gttgtttgaa tggtggaagt
     2281 gagccaggta ttgagaacga ggggcgcaag ggtgcgtgga agaaggcagg gagaggcgag
     2341 gcgaaggaga agggcatgtg gtgggaacga agcctccaca ccacagccac ccatgctttt
     2401 ctctgttcga gttcccgggt tctactctca cacactacac gtgcacagaa aacattcacg
     2461 cacaacagag atacagaggg atccgtcgag atggtctaca cgtcctaatg tcccctgctt
     2521 tcgaagccca gagccatcgc tttctgtttt tatccattcg caacacatcc tgttctcgcg
     2581 acctctcctc ttcctggcca ccatcgcttc tccgtgccat gccctgtaca tccttccctc
     2641 cctcgttgtc taccacacta atcatgcgct ctgcagcaga cactcggcta ctatttgtgt
     2701 cgtacggatt gaggggaggg aggggaaggg aggcaagaag taaatcggtg gtcaacaaca
     2761 gacgcggtct ccccaccctc cctcattcct ttcccctcct ataatgagcg aagaaagcca
     2821 tggacaccga gtcacccact ctcctaatcc accaccacca gggcactaac ggactcacgc
     2881 tcaccaaact cggtaacaat atccactgca cacagataca cacgcaaccc tcgagcatgc
     2941 ccaagaaaaa gcggaaggtg agcatctacc aggagttcgt caacaagtat tcactgagta
     3001 agacactgcg gttcgagctg atcccacagg gcaagacact ggagaacatc aaggcccgag
     3061 gcctgattct ggacgatgag aagcgggcaa aagactataa gaaagccaag cagatcattg
     3121 ataaatacca ccagttcttt atcgaggaaa ttctgagctc cgtgtgcatc agtgaggatc
     3181 tgctgcagaa ttactcagac gtgtacttca agctgaagaa gagcgacgat gacaacctgc
     3241 agaaggactt caagtccgcc aaggacacca tcaagaaaca gattagcgag tacatcaagg
     3301 actccgaaaa gtttaaaaat ctgttcaacc agaatctgat cgatgctaag aaaggccagg
     3361 agtccgacct gatcctgtgg ctgaaacagt ctaaggacaa tgggattgaa ctgttcaagg
     3421 ctaactccga tatcactgat attgacgagg cactggaaat catcaagagc ttcaagggat
     3481 ggaccacata ctttaaaggc ttccacgaga accgcaagaa cgtgtactcc agcaacgaca
     3541 ttcctacctc catcatctac cgaatcgtcg atgacaatct gccaaagttc ctggagaaca
     3601 aggccaaata tgaatctctg aaggacaaag ctcccgaggc aattaattac gaacagatca
     3661 agaaagatct ggctgaggaa ctgacattcg atatcgacta taagactagc gaggtgaacc
     3721 agagggtctt ttccctggac gaggtgtttg aaatcgccaa tttcaacaat tacctgaacc
     3781 agtccggcat tactaaattc aataccatca ttggcgggaa gtttgtgaac ggggagaata
     3841 ccaagcgcaa gggaattaac gaatacatca atctgtatag ccagcagatc aacgacaaaa
     3901 ctctgaagaa atacaagatg tctgtgctgt tcaaacagat cctgagtgat accgagtcca
     3961 agtcttttgt cattgataaa ctggaagatg actcagacgt ggtcactacc atgcagagct
     4021 tttatgagca gatcgccgct ttcaagacag tggaggaaaa atctattaag gaaactctga
     4081 gtctgctgtt cgatgacctg aaagcccaga agctggacct gagtaagatc tacttcaaaa
     4141 acgataagag tctgacagac ctgtcacagc aggtgtttga tgactattcc gtgattggga
     4201 ccgccgtcct ggagtacatt acacagcaga tcgctccaaa gaacctggat aatccctcta
     4261 agaaagagca ggaactgatc gctaagaaaa ccgagaaggc aaaatatctg agtctggaaa
     4321 caattaagct ggcactggag gagttcaaca agcacaggga tattgacaaa cagtgccgct
     4381 ttgaggaaat cctggccaac ttcgcagcca tccccatgat ttttgatgag atcgcccaga
     4441 acaaagacaa tctggctcag atcagtatta agtaccagaa ccagggcaag aaagacctgc
     4501 tgcaggcttc agcagaagat gacgtgaaag ccatcaagga tctgctggac cagaccaaca
     4561 atctgctgca caagctgaaa atcttccata ttagtcagtc agaggataag gctaatatcc
     4621 tggataaaga cgaacacttc tacctggtgt tcgaggaatg ttacttcgag ctggcaaaca
     4681 ttgtccccct gtataacaag attaggaact acatcacaca gaagccttac tctgacgaga
     4741 agtttaaact gaacttcgaa aatagtaccc tggccaacgg gtgggataag aacaaggagc
     4801 ctgacaacac agctatcctg ttcatcaagg atgacaagta ctatctggga gtgatgaata
     4861 agaaaaacaa taagatcttc gatgacaaag ccattaagga gaacaaaggg gaaggataca
     4921 agaaaatcgt gtataagctg ctgcccggcg caaataagat gctgcctaag gtgttcttca
     4981 gcgccaagag tatcaaattc tacaacccat ccgaggacat cctgcggatt agaaatcact
     5041 caacacatac taagaacggg agcccccaga agggatatga gaaatttgag ttcaacatcg
     5101 aggattgcag gaagtttatt gacttctaca agcagagcat ctccaaacac cctgaatgga
     5161 aggattttgg cttccggttt tccgacacac agagatataa ctctatcgac gagttctacc
     5221 gcgaggtgga aaatcagggg tataagctga cttttgagaa catttctgaa agttacatcg
     5281 acagcgtggt caatcaggga aagctgtacc tgttccagat ctataacaaa gatttttcag
     5341 catacagcaa gggcagacca aacctgcata cactgtactg gaaggccctg ttcgatgaga
     5401 ggaatctgca ggacgtggtc tataaactga acggagaggc cgaactgttt taccggaagc
     5461 agtctattcc taagaaaatc actcacccag ctaaggaggc catcgctaac aagaacaagg
     5521 acaatcctaa gaaagagagc gtgttcgaat acgatctgat taaggacaag cggttcaccg
     5581 aagataagtt ctttttccat tgtccaatca ccattaactt caagtcaagc ggcgctaaca
     5641 agttcaacga cgagatcaat ctgctgctga aggaaaaagc aaacgatgtg cacatcctga
     5701 gcattgaccg aggagagcgg catctggcct actataccct ggtggatggc aaagggaata
     5761 tcattaagca ggatacattc aacatcattg gcaatgaccg gatgaaaacc aactaccacg
     5821 ataaactggc tgcaatcgag aaggatagag actcagctag gaaggactgg aagaaaatca
     5881 acaacattaa ggagatgaag gaaggctatc tgagccaggt ggtccatgag attgcaaagc
     5941 tggtcatcga atacaatgcc attgtggtgt tcgaggatct gaacttcggc tttaagaggg
     6001 ggcgctttaa ggtggaaaaa caggtctatc agaagctgga gaaaatgctg atcgaaaagc
     6061 tgaattacct ggtgtttaaa gataacgagt tcgacaagac cggaggcgtc ctgagagcct
     6121 accagctgac agctcccttt gaaactttca agaaaatggg aaaacagaca ggcatcatct
     6181 actatgtgcc agccggattc acttccaaga tctgccccgt gaccggcttt gtcaaccagc
     6241 tgtaccctaa atatgagtca gtgagcaagt cccaggaatt tttcagcaag ttcgataaga
     6301 tctgttataa tctggacaag gggtacttcg agttttcctt cgattacaag aacttcggcg
     6361 acaaggccgc taaggggaaa tggaccattg cctccttcgg atctcgcctg atcaactttc
     6421 gaaattccga taaaaaccac aattgggaca ctagggaggt gtacccaacc aaggagctgg
     6481 aaaagctgct gaaagactac tctatcgagt atggacatgg cgaatgcatc aaggcagcca
     6541 tctgtggcga gagtgataag aaatttttcg ccaagctgac ctcagtgctg aatacaatcc
     6601 tgcagatgcg gaactcaaag accgggacag aactggacta tctgattagc cccgtggctg
     6661 atgtcaacgg aaacttcttc gacagcagac aggcacccaa aaatatgcct caggatgcag
     6721 acgccaacgg ggcctaccac atcgggctga agggactgat gctgctgggc cggatcaaga
     6781 acaatcagga ggggaagaag ctgaacctgg tcattaagaa cgaggaatac ttcgagtttg
     6841 tccagaatag aaataacggg tccggcagcg ggtccgttaa catggtgttt actctcgagg
     6901 acttcgtggg cgactggcgg cagacagctg gctataacct cgaccaggtg ctggagcagg
     6961 gcggtgtgtc ctcgcttttc cagaacctgg gcgtctccgt gaccccaatc cagcgcatcg
     7021 tcctgagcgg cgagaacggc ctgaagatcg acatccacgt catcattccc tacgagggcc
     7081 tgagcgggga ccaaatgggc cagatcgaga agatcttcaa ggttgtgtac cccgtggacg
     7141 atcaccactt caaggtgata ctgcactacg ggacgctggt catcgacggt gtgacgccga
     7201 acatgattga ctacttcggc cgtccttacg agggcattgc cgtgttcgac ggcaagaaga
     7261 tcacggtgac cggcaccctg tggaacggca ataagatcat tgacgagcgg ctgatcaacc
     7321 cggatggctc gctgttgttc cgcgtgacca tcaacggggt aaccggctgg cgcctgtgcg
     7381 agcgcatcct ggccggatac ccctacgacg tgcccgacta cgcgtccatg gtggctagcc
     7441 cgaaaaagaa gaggaaggtc tgacaattgg aaagatccaa gagagacgag tagagatttt
     7501 ttttttggga ttgatgtttg tcgttctttg agttgtcgtc gagttacgcc ttttgtaaga
     7561 atgttccgca ggagaggagg aggatgggca tgagtgaggg tgagagggct tgcccgcttt
     7621 tttttttaaa aacgctgaag acgtggttgt caaacaaacc ccccatagaa acgattttgt
     7681 tacggtgcgg tccagacgtc acttgaatgg ctccgcggaa aggccaggga gggaaggggg
     7741 gagggaggaa acatgaaaca tgttgaacgg ctcaacaggg tttgggggac aagagaggta
     7801 gcgccctgat ggactgctcc ctcccctcct ttccctcaat gtctcattca tccatgcttc
     7861 ccccttctct ctctcccctc cgttccatcc cccgcgggcg tggtagtggc gtgatgggat
     7921 ccactaaaat gtacgtgtaa gaaaagccgg tgagcttacg cttttgtgaa agtgggagta
     7981 cgagtgttgt gtgtgtgtgt agtggtttca gaccccagac agaggcgaag cagaaaaagc
     8041 agacgatgaa gacgacgaag aaatgagcag tctattttta tcgtggaaac agaagaggtg
     8101 atatcgtctc ctgcaggcat gcaagctgat ccactagagg ccatggcggc cgcgagggtg
     8161 gacttgagag tgtggaagtg agtgagggta gaagatagag taaggatggc agacaagatg
     8221 acgataaaga agacgtggaa gagagggaaa tacacgtaca aaggaagaaa acaagaaaag
     8281 gaaaaagagg aaaaaataaa cgcaggcgga attactcact gttgaaatgg tctctcgtcc
     8341 tgacacggaa gttgtacgaa caacacgcat gcacacaacg tactcttctt ctgtgttcta
     8401 aataaagact atttgctaag ggagggtagt ggcgatggtg gcgtacagtg acttaatggg
     8461 gaggagcaaa tcaatgatgc cttttttggc atttttctat tgtctatctg cggtactaca
     8521 attcgttgtt atacagtcac ggatggaagg aagaagcagc agtagagtag gggataaacc
     8581 aatgagataa gggagctgat gtaacgagac caggacgcct gaaattaaag atagaaaaac
     8641 cacaagtaga gccaggagcg atgaccatct tccatttcgt ttcttcacaa cagtgctttt
     8701 gctttcttcg cttctactct ctccttactg gctaccatga cattgttcac ttctttcatt
     8761 tctccttccc tctctcacac acatcccacc ccttcgattt ccttctgccc ttcttccctc
     8821 ggggtctagt cttattttga ttgaaccgat gcgtgaattg gatcgagtga cttgtatgac
     8881 gggcacacac acacatgacg acgacaggtg aggacttgaa ggacctaagg catgacacct
     8941 aaaaggaagg aaggccgaaa gacgacggca gaagtgggga tctctgaaac cagatccctt
     9001 ctcctcttca ctccctcctc ctgatccctg gccatcagtc ctgctcctcg gccacgaagt
     9061 gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga
     9121 tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact
     9181 cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca
     9241 cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt
     9301 cgtcctccac gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg
     9361 cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa cttggccatc tgctcggagg
     9421 ggaggatgaa gggaaggagg gaggggggag gggcatttat gacaattaga acttcagagg
     9481 tctactcctt gtgtgtaacg aggtgaacga cgggtgaaag tgttcgcctg acaggactgg
     9541 tcgaagatcg gggggagggg gctacctcta tctctgaggg catctttctg ataaggtgcc
     9601 ttcaaccgca accccgaaaa cccctgcccc ccaccacacc atggacacat gcacgcacga
     9661 caaatagaca gagcgatgcc aacaggattg gttgtatagt atggttctgg taagatagag
     9721 ggattaaatg ccctgctcca ggccgtgagg ggcgaactgg cgcgcttgtg cttctccttc
     9781 tgtacttaat ctacatccct gaccttgaac tggatcttga caaatataga tccgaaaacc
     9841 cactgccgca cgaacttacc ataatttaaa aagaggtttt agaggcgaag agtggagact
     9901 tctgtgtgag gtttgtgaaa aggtaagaag gaggggacag gagatcatga atctatatct
     9961 taattaagca cttctgctgt tttgaatggg gttagagcac aagtgggatg cctggacttt
    10021 cagggcatgc aagcacacac tacgaccaca acagaagtca aaagggtgta gatagagttt
    10081 ggcctcttgt ctgatgggca ttcacccggc agtatcctag ttaaaacaaa gacacatgaa
    10141 gagatataaa gatcatcgcg tgtgccctct agacgggcgg gctatagagg aaaggacaaa
    10201 agaccgccaa accgacgcag gatgcggaca ggttcagtgc accaaggagg aggaaacgcc
    10261 caaccacgag gtccaaggct ggcagagaaa taaacgaccc cgtttaaaca tgtgagcaaa
    10321 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10381 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10441 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    10501 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10561 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10621 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10681 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10741 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10801 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10861 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10921 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10981 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    11041 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    11101 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    11161 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    11221 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    11281 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    11341 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11401 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    11461 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    11521 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    11581 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    11641 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    11701 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11761 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11821 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11881 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11941 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    12001 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    12061 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg
    12121 gtccttttca tcacgtgcta taaaaataat tataatttaa att
//
