LOCUS       sequence-347037-       11576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the humanized fnCas12a gene sequence
            without Nlux tag and crRNA targeting the Nitrate reductase gene of 
            N. oceanica IMET1 with spacer 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11576)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 11576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_hfnCas12a_crRNA NR3
FEATURES             Location/Qualifiers
     source          1..11576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        486..590
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(591..659,660..1451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(809..828)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1622..2210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2111..2130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3170..3544
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      4429..4438
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5119..5139)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5144..5153
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5155..5181)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(9079..9099)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9320..9331)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     ncRNA           10328..10364
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     ncRNA           10426..10493
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     misc_feature    join(11522..11576,1..449)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 gggaatttac tctgtgttta tttattttta tgttttgtat ttggatttta gaaagtaaat
       61 aaagaaggta gaagagttac ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt
      121 caacaaaaag cgtactttac atatatattt attagacaag aaaagcagat taaatagata
      181 tacattcgat taacgataag taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta
      241 cacagacaag atgaaacaat tcggcattaa tacctgagag caggaagtac aagataaaag
      301 gtagtatttg ttggcgatcc ccctagagtc ttttacatct tcggaaaaca aaaactattt
      361 tttctttaat ttcttttttt actttctatt tttaatttat atatttatat taaaaaattt
      421 aaattataat tatttttata gcacgtgatg aaaaggaccc aggtggcact tttcggggaa
      481 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      541 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      601 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      661 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      721 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      781 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      841 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
      901 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
      961 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1021 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1081 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     1141 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1201 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1261 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1321 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1381 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1441 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1501 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1561 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1621 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1681 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1741 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1801 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1861 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1921 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1981 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2041 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2101 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2161 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2221 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tttaaacggg
     2281 gtcgtttatt tctctgccag ccttggacct cgtggttggg cgtttcctcc tccttggtgc
     2341 actgaacctg tccgcatcct gcgtcggttt ggcggtcttt tgtcctttcc tctatagccc
     2401 gcccgtctag agggcacacg cgatgatctt tatatctctt catgtgtctt tgttttaact
     2461 aggatactgc cgggtgaatg cccatcagac aagaggccaa actctatcta cacccttttg
     2521 acttctgttg tggtcgtagt gtgtgcttgc atgccctgaa agtccaggca tcccacttgt
     2581 gctctaaccc cattcaaaac agcagaagtg cttaattaag atatagattc atgatctcct
     2641 gtcccctcct tcttaccttt tcacaaacct cacacagaag tctccactct tcgcctctaa
     2701 aacctctttt taaattatgg taagttcgtg cggcagtggg ttttcggatc tatatttgtc
     2761 aagatccagt tcaaggtcag ggatgtagat taagtacaga aggagaagca caagcgcgcc
     2821 agttcgcccc tcacggcctg gagcagggca tttaatccct ctatcttacc agaaccatac
     2881 tatacaacca atcctgttgg catcgctctg tctatttgtc gtgcgtgcat gtgtccatgg
     2941 tgtggtgggg ggcaggggtt ttcggggttg cggttgaagg caccttatca gaaagatgcc
     3001 ctcagagata gaggtagccc cctccccccg atcttcgacc agtcctgtca ggcgaacact
     3061 ttcacccgtc gttcacctcg ttacacacaa ggagtagacc tctgaagttc taattgtcat
     3121 aaatgcccct cccccctccc tccttccctt catcctcccc tccgagcaga tggccaagtt
     3181 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     3241 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     3301 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     3361 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     3421 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     3481 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     3541 ctgatggcca gggatcagga ggagggagtg aagaggagaa gggatctggt ttcagagatc
     3601 cccacttctg ccgtcgtctt tcggccttcc ttccttttag gtgtcatgcc ttaggtcctt
     3661 caagtcctca cctgtcgtcg tcatgtgtgt gtgtgcccgt catacaagtc actcgatcca
     3721 attcacgcat cggttcaatc aaaataagac tagaccccga gggaagaagg gcagaaggaa
     3781 atcgaagggg tgggatgtgt gtgagagagg gaaggagaaa tgaaagaagt gaacaatgtc
     3841 atggtagcca gtaaggagag agtagaagcg aagaaagcaa aagcactgtt gtgaagaaac
     3901 gaaatggaag atggtcatcg ctcctggctc tacttgtggt ttttctatct ttaatttcag
     3961 gcgtcctggt ctcgttacat cagctccctt atctcattgg tttatcccct actctactgc
     4021 tgcttcttcc ttccatccgt gactgtataa caacgaattg tagtaccgca gatagacaat
     4081 agaaaaatgc caaaaaaggc atcattgatt tgctcctccc cattaagtca ctgtacgcca
     4141 ccatcgccac taccctccct tagcaaatag tctttattta gaacacagaa gaagagtacg
     4201 ttgtgtgcat gcgtgttgtt cgtacaactt ccgtgtcagg acgagagacc atttcaacag
     4261 tgagtaattc cgcctgcgtt tattttttcc tctttttcct tttcttgttt tcttcctttg
     4321 tacgtgtatt tccctctctt ccacgtcttc tttatcgtca tcttgtctgc catccttact
     4381 ctatcttcta ccctcactca cttccacact ctcaagtcca ccctcgcggc cgccatggcc
     4441 tctagtggat cagcttgcat gcctgcagga gacgatatca cctcttctgt ttccacgata
     4501 aaaatagact gctcatttct tcgtcgtctt catcgtctgc tttttctgct tcgcctctgt
     4561 ctggggtctg aaaccactac acacacacac aacactcgta ctcccacttt cacaaaagcg
     4621 taagctcacc ggcttttctt acacgtacat tttagtggat cccatcacgc cactaccacg
     4681 cccgcggggg atggaacgga ggggagagag agaaggggga agcatggatg aatgagacat
     4741 tgagggaaag gaggggaggg agcagtccat cagggcgcta cctctcttgt cccccaaacc
     4801 ctgttgagcc gttcaacatg tttcatgttt cctccctccc cccttccctc cctggccttt
     4861 ccgcggagcc attcaagtga cgtctggacc gcaccgtaac aaaatcgttt ctatgggggg
     4921 tttgtttgac aaccacgtct tcagcgtttt taaaaaaaaa agcgggcaag ccctctcacc
     4981 ctcactcatg cccatcctcc tcctctcctg cggaacattc ttacaaaagg cgtaactcga
     5041 cgacaactca aagaacgaca aacatcaatc ccaaaaaaaa aatctctact cgtctctctt
     5101 ggatctttcc aattgtcaga ccttcctctt ctttttcggg ctagccacca tggacgcgta
     5161 gtcgggcacg tcgtaggggt agttatttct attctggaca aactcgaagt attcctcgtt
     5221 cttaatgacc aggttcagct tcttcccctc ctgattgttc ttgatccggc ccagcagcat
     5281 cagtcccttc agcccgatgt ggtaggcccc gttggcgtct gcatcctgag gcatattttt
     5341 gggtgcctgt ctgctgtcga agaagtttcc gttgacatca gccacggggc taatcagata
     5401 gtccagttct gtcccggtct ttgagttccg catctgcagg attgtattca gcactgaggt
     5461 cagcttggcg aaaaatttct tatcactctc gccacagatg gctgccttga tgcattcgcc
     5521 atgtccatac tcgatagagt agtctttcag cagcttttcc agctccttgg ttgggtacac
     5581 ctccctagtg tcccaattgt ggtttttatc ggaatttcga aagttgatca ggcgagatcc
     5641 gaaggaggca atggtccatt tccccttagc ggccttgtcg ccgaagttct tgtaatcgaa
     5701 ggaaaactcg aagtacccct tgtccagatt ataacagatc ttatcgaact tgctgaaaaa
     5761 ttcctgggac ttgctcactg actcatattt agggtacagc tggttgacaa agccggtcac
     5821 ggggcagatc ttggaagtga atccggctgg cacatagtag atgatgcctg tctgttttcc
     5881 cattttcttg aaagtttcaa agggagctgt cagctggtag gctctcagga cgcctccggt
     5941 cttgtcgaac tcgttatctt taaacaccag gtaattcagc ttttcgatca gcattttctc
     6001 cagcttctga tagacctgtt tttccacctt aaagcgcccc ctcttaaagc cgaagttcag
     6061 atcctcgaac accacaatgg cattgtattc gatgaccagc tttgcaatct catggaccac
     6121 ctggctcaga tagccttcct tcatctcctt aatgttgttg attttcttcc agtccttcct
     6181 agctgagtct ctatccttct cgattgcagc cagtttatcg tggtagttgg ttttcatccg
     6241 gtcattgcca atgatgttga atgtatcctg cttaatgata ttccctttgc catccaccag
     6301 ggtatagtag gccagatgcc gctctcctcg gtcaatgctc aggatgtgca catcgtttgc
     6361 tttttccttc agcagcagat tgatctcgtc gttgaacttg ttagcgccgc ttgacttgaa
     6421 gttaatggtg attggacaat ggaaaaagaa cttatcttcg gtgaaccgct tgtccttaat
     6481 cagatcgtat tcgaacacgc tctctttctt aggattgtcc ttgttcttgt tagcgatggc
     6541 ctccttagct gggtgagtga ttttcttagg aatagactgc ttccggtaaa acagttcggc
     6601 ctctccgttc agtttataga ccacgtcctg cagattcctc tcatcgaaca gggccttcca
     6661 gtacagtgta tgcaggtttg gtctgccctt gctgtatgct gaaaaatctt tgttatagat
     6721 ctggaacagg tacagctttc cctgattgac cacgctgtcg atgtaacttt cagaaatgtt
     6781 ctcaaaagtc agcttatacc cctgattttc cacctcgcgg tagaactcgt cgatagagtt
     6841 atatctctgt gtgtcggaaa accggaagcc aaaatccttc cattcagggt gtttggagat
     6901 gctctgcttg tagaagtcaa taaacttcct gcaatcctcg atgttgaact caaatttctc
     6961 atatcccttc tgggggctcc cgttcttagt atgtgttgag tgatttctaa tccgcaggat
     7021 gtcctcggat gggttgtaga atttgatact cttggcgctg aagaacacct taggcagcat
     7081 cttatttgcg ccgggcagca gcttatacac gattttcttg tatccttccc ctttgttctc
     7141 cttaatggct ttgtcatcga agatcttatt gtttttctta ttcatcactc ccagatagta
     7201 cttgtcatcc ttgatgaaca ggatagctgt gttgtcaggc tccttgttct tatcccaccc
     7261 gttggccagg gtactatttt cgaagttcag tttaaacttc tcgtcagagt aaggcttctg
     7321 tgtgatgtag ttcctaatct tgttatacag ggggacaatg tttgccagct cgaagtaaca
     7381 ttcctcgaac accaggtaga agtgttcgtc tttatccagg atattagcct tatcctctga
     7441 ctgactaata tggaagattt tcagcttgtg cagcagattg ttggtctggt ccagcagatc
     7501 cttgatggct ttcacgtcat cttctgctga agcctgcagc aggtctttct tgccctggtt
     7561 ctggtactta atactgatct gagccagatt gtctttgttc tgggcgatct catcaaaaat
     7621 catggggatg gctgcgaagt tggccaggat ttcctcaaag cggcactgtt tgtcaatatc
     7681 cctgtgcttg ttgaactcct ccagtgccag cttaattgtt tccagactca gatattttgc
     7741 cttctcggtt ttcttagcga tcagttcctg ctctttctta gagggattat ccaggttctt
     7801 tggagcgatc tgctgtgtaa tgtactccag gacggcggtc ccaatcacgg aatagtcatc
     7861 aaacacctgc tgtgacaggt ctgtcagact cttatcgttt ttgaagtaga tcttactcag
     7921 gtccagcttc tgggctttca ggtcatcgaa cagcagactc agagtttcct taatagattt
     7981 ttcctccact gtcttgaaag cggcgatctg ctcataaaag ctctgcatgg tagtgaccac
     8041 gtctgagtca tcttccagtt tatcaatgac aaaagacttg gactcggtat cactcaggat
     8101 ctgtttgaac agcacagaca tcttgtattt cttcagagtt ttgtcgttga tctgctggct
     8161 atacagattg atgtattcgt taattccctt gcgcttggta ttctccccgt tcacaaactt
     8221 cccgccaatg atggtattga atttagtaat gccggactgg ttcaggtaat tgttgaaatt
     8281 ggcgatttca aacacctcgt ccagggaaaa gaccctctgg ttcacctcgc tagtcttata
     8341 gtcgatatcg aatgtcagtt cctcagccag atctttcttg atctgttcgt aattaattgc
     8401 ctcgggagct ttgtccttca gagattcata tttggccttg ttctccagga actttggcag
     8461 attgtcatcg acgattcggt agatgatgga ggtaggaatg tcgttgctgg agtacacgtt
     8521 cttgcggttc tcgtggaagc ctttaaagta tgtggtccat cccttgaagc tcttgatgat
     8581 ttccagtgcc tcgtcaatat cagtgatatc ggagttagcc ttgaacagtt caatcccatt
     8641 gtccttagac tgtttcagcc acaggatcag gtcggactcc tggcctttct tagcatcgat
     8701 cagattctgg ttgaacagat ttttaaactt ttcggagtcc ttgatgtact cgctaatctg
     8761 tttcttgatg gtgtccttgg cggacttgaa gtccttctgc aggttgtcat cgtcgctctt
     8821 cttcagcttg aagtacacgt ctgagtaatt ctgcagcaga tcctcactga tgcacacgga
     8881 gctcagaatt tcctcgataa agaactggtg gtatttatca atgatctgct tggctttctt
     8941 atagtctttt gcccgcttct catcgtccag aatcaggcct cgggccttga tgttctccag
     9001 tgtcttgccc tgtgggatca gctcgaaccg cagtgtctta ctcagtgaat acttgttgac
     9061 gaactcctgg tagatgctca ccttccgctt tttcttgggc atgctcgagg gttgcgtgtg
     9121 tatctgtgtg cagtggatat tgttaccgag tttggtgagc gtgagtccgt tagtgccctg
     9181 gtggtggtgg attaggagag tgggtgactc ggtgtccatg gctttcttcg ctcattatag
     9241 gaggggaaag gaatgaggga gggtggggag accgcgtctg ttgttgacca ccgatttact
     9301 tcttgcctcc cttcccctcc ctcccctcaa tccgtacgac acaaatagta gccgagtgtc
     9361 tgctgcagag cgcatgatta gtgtggtaga caacgaggga gggaaggatg tacagggcat
     9421 ggcacggaga agcgatggtg gccaggaaga ggagaggtcg cgagaacagg atgtgttgcg
     9481 aatggataaa aacagaaagc gatggctctg ggcttcgaaa gcaggggaca ttaggacgtg
     9541 tagaccatct cgacggatcc ctctgtatct ctgttgtgcg tgaatgtttt ctgtgcacgt
     9601 gtagtgtgtg agagtagaac ccgggaactc gaacagagaa aagcatgggt ggctgtggtg
     9661 tggaggcttc gttcccacca catgcccttc tccttcgcct cgcctctccc tgccttcttc
     9721 cacgcaccct tgcgcccctc gttctcaata cctggctcac ttccaccatt caaacaacca
     9781 tcacgataca ggcatttatc tatcgttgaa gacttcttcc tccggtagat cttagccaag
     9841 gtaagaagag gggcatgcag caaggagaaa gaaatgatgc atgatgagga atagaagggg
     9901 aggagggagg gatatgatgg gaagcgaaag cgcatattct ggtggtctgc tgcctgatgg
     9961 ggacgcgtct agctgtgaca ctgaggacgg tggctgctgg tggctgcggg cgctgccttg
    10021 gtgatcaatg ggagtaaagg gagggaagga ggtagcgtga acggatgacg cggagaagtt
    10081 taggggtctc tttacgtatc gcccctgccg cccgcctctc tgcgataaat gtgcctgtta
    10141 ccctgcagcc tctattcttc actgtgttcc tgttttccaa cagcctctat tcttccctgt
    10201 cttttgttgc agtggcgtca tcctctcttt gccccagtcg tcgttctctc gactcactca
    10261 ctccccccct ccttccctcc ctccatccac agaatcgatg aagagcaggc cttggctctt
    10321 cttagacctg atgagtccgt gaggacgaaa cgagtaagct cgtcgtctaa gaactttaaa
    10381 taatttctac tgttgtagat cagtctcgtc ggcccctgcc tccatggccg gcatggtccc
    10441 agcctcctcg ctggcgccgg ctgggcaaca tgcttcggca tggcgaatgg gactcctggg
    10501 taccatggga aagaaaggat gagaaaggag aaaggacatc tagataaccg gcatatcacg
    10561 gtggtgtatt agtgtagaat agtgaagaga agacttggga aaatgtgtag gaaaggttgt
    10621 ttctgtgtat gtgggttggg atgggtggct gtttgagaag gaacagcggg cagggcgatg
    10681 tagtgctgaa cgggcacgga accactgaga ctgaaggaag tagggagaga gaggggcagg
    10741 ggacgtgcac tttaatcttt gcctcggtag agtataccca tgcaagagta tgtggccacc
    10801 tgtggtggct tttggccagg tctggtgcag tgccaatcat ctcccatcaa taatacaact
    10861 tcagaacaac ggcgcattga tggggagaga gaaagtaaat ttaagtaagg ggtacgtagt
    10921 agaggattca actgaaatat tttcgaggag cggttgggaa gttgaccgat tgaaaggaga
    10981 agggagggga gcaggtgtga tagtcatgtg taaagtaatt cttttttgcc gtcgtcacac
    11041 aatccacatc aatgataaaa tatgtttaag gatcaatcac acggagtcgg tcataaggca
    11101 accgcaaacg caatgcaaac tagcaagcaa gcaggaccac aacaacaacc attaccatca
    11161 caggcgacag cagcagcagc tgcagcagca gcaaggcaag caaaggccat ctgcgcgtgg
    11221 acttttccag gcaccggctt taagcgtaat tttcaatgat tgttgtccgt gtatcctctc
    11281 accctttcac cgcttgcgcg cacgtgagca gcacaactgg taatgcccga ggacaataga
    11341 cgaggaaaaa gaagaagaaa aatgaagagg gagtgatgaa gaagaagaga agaataaaag
    11401 aagacagaaa agaagataaa aaagtcaaaa gacgacaaag acggaaaaaa aagaaacgcg
    11461 caattttaac accagcaacg acgacgaaaa gggcgtcagc tttgcaccgg tttcttagac
    11521 ggatcgcttg cctgtaactt acacgcgcct cgtatctttt aatgatggaa taattt
//
