LOCUS       sequence-347038-       11371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the dead version of humanized 
            fnCas12a gene sequence tagged with Nlux and crRNA targeting the 
            fluorescent gene tdtomato with spacer 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11371)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 11371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_dfnCas12a_crRNA Td2
FEATURES             Location/Qualifiers
     source          1..11371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     ncRNA           complement(1433..1500)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(1545..1581)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             2563..2574
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2795..2815
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7253..7279
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      7281..7290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7295..7315
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      7997..8006
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     rep_origin      complement(9511..10099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9591..9610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10270..11061,11062..11130))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10893..10912
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11131..11235)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(11272..11371,1..404)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 gttttccgaa gatgtaaaag actctagggg gatcgccaac aaatactacc ttttatcttg
       61 tacttcctgc tctcaggtat taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac
      121 acacgaaaat cctgtgattt tacattttac ttatcgttaa tcgaatgtat atctatttaa
      181 tctgcttttc ttgtctaata aatatatatg taaagtacgc tttttgttga aattttttaa
      241 acctttgttt attttttttt cttcattccg taactcttct accttcttta tttactttct
      301 aaaatccaaa tacaaaacat aaaaataaat aaacacagag taaattccca aattattcca
      361 tcattaaaag atacgaggcg cgtgtaagtt acaggcaagc gatccgtcta agaaaccggt
      421 gcaaagctga cgcccttttc gtcgtcgttg ctggtgttaa aattgcgcgt ttcttttttt
      481 tccgtctttg tcgtcttttg acttttttat cttcttttct gtcttctttt attcttctct
      541 tcttcttcat cactccctct tcatttttct tcttcttttt cctcgtctat tgtcctcggg
      601 cattaccagt tgtgctgctc acgtgcgcgc aagcggtgaa agggtgagag gatacacgga
      661 caacaatcat tgaaaattac gcttaaagcc ggtgcctgga aaagtccacg cgcagatggc
      721 ctttgcttgc cttgctgctg ctgcagctgc tgctgctgtc gcctgtgatg gtaatggttg
      781 ttgttgtggt cctgcttgct tgctagtttg cattgcgttt gcggttgcct tatgaccgac
      841 tccgtgtgat tgatccttaa acatatttta tcattgatgt ggattgtgtg acgacggcaa
      901 aaaagaatta ctttacacat gactatcaca cctgctcccc tcccttctcc tttcaatcgg
      961 tcaacttccc aaccgctcct cgaaaatatt tcagttgaat cctctactac gtacccctta
     1021 cttaaattta ctttctctct ccccatcaat gcgccgttgt tctgaagttg tattattgat
     1081 gggagatgat tggcactgca ccagacctgg ccaaaagcca ccacaggtgg ccacatactc
     1141 ttgcatgggt atactctacc gaggcaaaga ttaaagtgca cgtcccctgc ccctctctct
     1201 ccctacttcc ttcagtctca gtggttccgt gcccgttcag cactacatcg ccctgcccgc
     1261 tgttccttct caaacagcca cccatcccaa cccacataca cagaaacaac ctttcctaca
     1321 cattttccca agtcttctct tcactattct acactaatac accaccgtga tatgccggtt
     1381 atctagatgt cctttctcct ttctcatcct ttctttccca tggtacccag gagtcccatt
     1441 cgccatgccg aagcatgttg cccagccggc gccagcgagg aggctgggac catgccggcc
     1501 ccgaggacaa caacatggcc gtcatatcta caacagtaga aattgacgag cttactcgtt
     1561 tcgtcctcac ggactcatca gaatttcgct cttcatcgat tctgtggatg gagggaggga
     1621 aggagggggg gagtgagtga gtcgagagaa cgacgactgg ggcaaagaga ggatgacgcc
     1681 actgcaacaa aagacaggga agaatagagg ctgttggaaa acaggaacac agtgaagaat
     1741 agaggctgca gggtaacagg cacatttatc gcagagaggc gggcggcagg ggcgatacgt
     1801 aaagagaccc ctaaacttct ccgcgtcatc cgttcacgct acctccttcc ctccctttac
     1861 tcccattgat caccaaggca gcgcccgcag ccaccagcag ccaccgtcct cagtgtcaca
     1921 gctagacgcg tccccatcag gcagcagacc accagaatat gcgctttcgc ttcccatcat
     1981 atccctccct cctccccttc tattcctcat catgcatcat ttctttctcc ttgctgcatg
     2041 cccctcttct taccttggct aagatctacc ggaggaagaa gtcttcaacg atagataaat
     2101 gcctgtatcg tgatggttgt ttgaatggtg gaagtgagcc aggtattgag aacgaggggc
     2161 gcaagggtgc gtggaagaag gcagggagag gcgaggcgaa ggagaagggc atgtggtggg
     2221 aacgaagcct ccacaccaca gccacccatg cttttctctg ttcgagttcc cgggttctac
     2281 tctcacacac tacacgtgca cagaaaacat tcacgcacaa cagagataca gagggatccg
     2341 tcgagatggt ctacacgtcc taatgtcccc tgctttcgaa gcccagagcc atcgctttct
     2401 gtttttatcc attcgcaaca catcctgttc tcgcgacctc tcctcttcct ggccaccatc
     2461 gcttctccgt gccatgccct gtacatcctt ccctccctcg ttgtctacca cactaatcat
     2521 gcgctctgca gcagacactc ggctactatt tgtgtcgtac ggattgaggg gagggagggg
     2581 aagggaggca agaagtaaat cggtggtcaa caacagacgc ggtctcccca ccctccctca
     2641 ttcctttccc ctcctataat gagcgaagaa agccatggac accgagtcac ccactctcct
     2701 aatccaccac caccagggca ctaacggact cacgctcacc aaactcggta acaatatcca
     2761 ctgcacacag atacacacgc aaccctcgag catgcccaag aaaaagcgga aggtgagcat
     2821 ctaccaggag ttcgtcaaca agtattcact gagtaagaca ctgcggttcg agctgatccc
     2881 acagggcaag acactggaga acatcaaggc ccgaggcctg attctggacg atgagaagcg
     2941 ggcaaaagac tataagaaag ccaagcagat cattgataaa taccaccagt tctttatcga
     3001 ggaaattctg agctccgtgt gcatcagtga ggatctgctg cagaattact cagacgtgta
     3061 cttcaagctg aagaagagcg acgatgacaa cctgcagaag gacttcaagt ccgccaagga
     3121 caccatcaag aaacagatta gcgagtacat caaggactcc gaaaagttta aaaatctgtt
     3181 caaccagaat ctgatcgatg ctaagaaagg ccaggagtcc gacctgatcc tgtggctgaa
     3241 acagtctaag gacaatggga ttgaactgtt caaggctaac tccgatatca ctgatattga
     3301 cgaggcactg gaaatcatca agagcttcaa gggatggacc acatacttta aaggcttcca
     3361 cgagaaccgc aagaacgtgt actccagcaa cgacattcct acctccatca tctaccgaat
     3421 cgtcgatgac aatctgccaa agttcctgga gaacaaggcc aaatatgaat ctctgaagga
     3481 caaagctccc gaggcaatta attacgaaca gatcaagaaa gatctggctg aggaactgac
     3541 attcgatatc gactataaga ctagcgaggt gaaccagagg gtcttttccc tggacgaggt
     3601 gtttgaaatc gccaatttca acaattacct gaaccagtcc ggcattacta aattcaatac
     3661 catcattggc gggaagtttg tgaacgggga gaataccaag cgcaagggaa ttaacgaata
     3721 catcaatctg tatagccagc agatcaacga caaaactctg aagaaataca agatgtctgt
     3781 gctgttcaaa cagatcctga gtgataccga gtccaagtct tttgtcattg ataaactgga
     3841 agatgactca gacgtggtca ctaccatgca gagcttttat gagcagatcg ccgctttcaa
     3901 gacagtggag gaaaaatcta ttaaggaaac tctgagtctg ctgttcgatg acctgaaagc
     3961 ccagaagctg gacctgagta agatctactt caaaaacgat aagagtctga cagacctgtc
     4021 acagcaggtg tttgatgact attccgtgat tgggaccgcc gtcctggagt acattacaca
     4081 gcagatcgct ccaaagaacc tggataatcc ctctaagaaa gagcaggaac tgatcgctaa
     4141 gaaaaccgag aaggcaaaat atctgagtct ggaaacaatt aagctggcac tggaggagtt
     4201 caacaagcac agggatattg acaaacagtg ccgctttgag gaaatcctgg ccaacttcgc
     4261 agccatcccc atgatttttg atgagatcgc ccagaacaaa gacaatctgg ctcagatcag
     4321 tattaagtac cagaaccagg gcaagaaaga cctgctgcag gcttcagcag aagatgacgt
     4381 gaaagccatc aaggatctgc tggaccagac caacaatctg ctgcacaagc tgaaaatctt
     4441 ccatattagt cagtcagagg ataaggctaa tatcctggat aaagacgaac acttctacct
     4501 ggtgttcgag gaatgttact tcgagctggc aaacattgtc cccctgtata acaagattag
     4561 gaactacatc acacagaagc cttactctga cgagaagttt aaactgaact tcgaaaatag
     4621 taccctggcc aacgggtggg ataagaacaa ggagcctgac aacacagcta tcctgttcat
     4681 caaggatgac aagtactatc tgggagtgat gaataagaaa aacaataaga tcttcgatga
     4741 caaagccatt aaggagaaca aaggggaagg atacaagaaa atcgtgtata agctgctgcc
     4801 cggcgcaaat aagatgctgc ctaaggtgtt cttcagcgcc aagagtatca aattctacaa
     4861 cccatccgag gacatcctgc ggattagaaa tcactcaaca catactaaga acgggagccc
     4921 ccagaaggga tatgagaaat ttgagttcaa catcgaggat tgcaggaagt ttattgactt
     4981 ctacaagcag agcatctcca aacaccctga atggaaggat tttggcttcc ggttttccga
     5041 cacacagaga tataactcta tcgacgagtt ctaccgcgag gtggaaaatc aggggtataa
     5101 gctgactttt gagaacattt ctgaaagtta catcgacagc gtggtcaatc agggaaagct
     5161 gtacctgttc cagatctata acaaagattt ttcagcatac agcaagggca gaccaaacct
     5221 gcatacactg tactggaagg ccctgttcga tgagaggaat ctgcaggacg tggtctataa
     5281 actgaacgga gaggccgaac tgttttaccg gaagcagtct attcctaaga aaatcactca
     5341 cccagctaag gaggccatcg ctaacaagaa caaggacaat cctaagaaag agagcgtgtt
     5401 cgaatacgat ctgattaagg acaagcggtt caccgaagat aagttctttt tccattgtcc
     5461 aatcaccatt aacttcaagt caagcggcgc taacaagttc aacgacgaga tcaatctgct
     5521 gctgaaggaa aaagcaaacg atgtgcacat cctgagcatt gcccgaggag agcggcatct
     5581 ggcctactat accctggtgg atggcaaagg gaatatcatt aagcaggata cattcaacat
     5641 cattggcaat gaccggatga aaaccaacta ccacgataaa ctggctgcaa tcgagaagga
     5701 tagagactca gctaggaagg actggaagaa aatcaacaac attaaggaga tgaaggaagg
     5761 ctatctgagc caggtggtcc atgagattgc aaagctggtc atcgaataca atgccattgt
     5821 ggtgttcgcg gatctgaact tcggctttaa gagggggcgc tttaaggtgg aaaaacaggt
     5881 ctatcagaag ctggagaaaa tgctgatcga aaagctgaat tacctggtgt ttaaagataa
     5941 cgagttcgac aagaccggag gcgtcctgag agcctaccag ctgacagctc cctttgaaac
     6001 tttcaagaaa atgggaaaac agacaggcat catctactat gtgccagccg gattcacttc
     6061 caagatctgc cccgtgaccg gctttgtcaa ccagctgtac cctaaatatg agtcagtgag
     6121 caagtcccag gaatttttca gcaagttcga taagatctgt tataatctgg acaaggggta
     6181 cttcgagttt tccttcgatt acaagaactt cggcgacaag gccgctaagg ggaaatggac
     6241 cattgcctcc ttcggatctc gcctgatcaa ctttcgaaat tccgataaaa accacaattg
     6301 ggacactagg gaggtgtacc caaccaagga gctggaaaag ctgctgaaag actactctat
     6361 cgagtatgga catggcgaat gcatcaaggc agccatctgt ggcgagagtg ataagaaatt
     6421 tttcgccaag ctgacctcag tgctgaatac aatcctgcag atgcggaact caaagaccgg
     6481 gacagaactg gactatctga ttagccccgt ggctgatgtc aacggaaact tcttcgacag
     6541 cagacaggca cccaaaaata tgcctcagga tgcagacgcc aacggggcct accacatcgg
     6601 gctgaaggga ctgatgctgc tgggccggat caagaacaat caggagggga agaagctgaa
     6661 cctggtcatt aagaacgagg aatacttcga gtttgtccag aatagaaata acgggtccgg
     6721 cagcgggtcc gttaacatgg tgtttactct cgaggacttc gtgggcgact ggcggcagac
     6781 agctggctat aacctcgacc aggtgctgga gcagggcggt gtgtcctcgc ttttccagaa
     6841 cctgggcgtc tccgtgaccc caatccagcg catcgtcctg agcggcgaga acggcctgaa
     6901 gatcgacatc cacgtcatca ttccctacga gggcctgagc ggggaccaaa tgggccagat
     6961 cgagaagatc ttcaaggttg tgtaccccgt ggacgatcac cacttcaagg tgatactgca
     7021 ctacgggacg ctggtcatcg acggtgtgac gccgaacatg attgactact tcggccgtcc
     7081 ttacgagggc attgccgtgt tcgacggcaa gaagatcacg gtgaccggca ccctgtggaa
     7141 cggcaataag atcattgacg agcggctgat caacccggat ggctcgctgt tgttccgcgt
     7201 gaccatcaac ggggtaaccg gctggcgcct gtgcgagcgc atcctggccg gataccccta
     7261 cgacgtgccc gactacgcgt ccatggtggc tagcccgaaa aagaagagga aggtctgaca
     7321 attggaaaga tccaagagag acgagtagag attttttttt tgggattgat gtttgtcgtt
     7381 ctttgagttg tcgtcgagtt acgccttttg taagaatgtt ccgcaggaga ggaggaggat
     7441 gggcatgagt gagggtgaga gggcttgccc gctttttttt tttaaaaacg ctgaagacgt
     7501 ggttgtcaaa caaacccccc atagaaacga ttttgttacg gtgcggtcca gacgtcactt
     7561 gaatggctcc gcggaaaggc cagggaggga aggggggagg gaggaaacat gaaacatgtt
     7621 gaacggctca acagggtttg ggggacaaga gaggtagcgc cctgatggac tgctccctcc
     7681 cctcctttcc ctcaatgtct cattcatcca tgcttccccc ttctctctct cccctccgtt
     7741 ccatcccccg cgggcgtggt agtggcgtga tgggatccac taaaatgtac gtgtaagaaa
     7801 agccggtgag cttacgcttt tgtgaaagtg ggagtacgag tgttgtgtgt gtgtgtagtg
     7861 gtttcagacc ccagacagag gcgaagcaga aaaagcagac gatgaagacg acgaagaaat
     7921 gagcagtcta tttttatcgt ggaaacagaa gaggtgatat cgtctcctgc aggcatgcaa
     7981 gctgatccac tagaggccat ggcggccgcg atggagtgga tggaggagga ggcgagcgta
     8041 gcagcaagcg tgagttatac agccaggcac atgtcgcaat ccttcggtct cgggcttaaa
     8101 atccacgcac taatcacgct gggccatgca aagagcaatg ccgaggccca ccacacaaaa
     8161 cgctgtgtcg cgcgttgcgg cctgaagctt catacttctt agtcgccgcc aaaagggctc
     8221 gagagacgag acccgttggc atgaccgatg ttgttcgacg cggtttgctt cgtcacagtc
     8281 gacgtgattc aggaatctgg agcctgcaga tcattttttt cagcctgata tcgttctttt
     8341 ccactgagaa ccatcagacc accttttctt ccattgtgtg aaggagtagg agttgccgtg
     8401 ctgctttgtg ggagacatct gcgatggtga ccagcctccc gtcgtctggt cgacgtgacg
     8461 agcctcttca ctgttcttcg acggagagac gcaagcgaga cggctctaga ccttttggac
     8521 acgcattctg tgtgtgaact agtggacagt gataccacgt ctgaaagctc accactgccc
     8581 atggtgcagc tacttgtcac aaagttttga ctccgtcggt atcaccattc gcgctcgtgt
     8641 gcctggttgt tccgccacgc cggcctgccc cggggcgggg caatattcta aaatctcacg
     8701 caaaacaccg cacttacccc tcacacatat tcgtgataga ccaccaccaa tctcagcccg
     8761 catcaacaca ggagggccca tgcccctctc gcaggaggag agcacgctga tcgagagggc
     8821 tacggcgacg attaactcga ttcctatctc cgaggattac tcggtggctt ccgctgctct
     8881 gtcgtccgac ggacggatct tcacgggggt caacgtgtac cattttacgg gggggccttg
     8941 cgcggagctg gtggtcctgg ggaccgccgc tgctgccgcg gctgggaacc tgacgtgcat
     9001 tgtcgccatc gggaacgaga accgcgggat tctgagcccc tgcgggcggt gccggcaggt
     9061 cctgctcgac ctccaccctg ggatcaaggc gattgtgaag gattccgacg ggcagcctac
     9121 ggcggtcggt atcagagaac ttctcccttc gggctacgtg tgggagagcg cttgaactag
     9181 tagtagatgc cgaccggagt ccgcaaaaat caccagtctc tctctacaaa tctatctctc
     9241 tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta gggttcttat
     9301 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
     9361 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacc tggtttaaac
     9421 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9481 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9541 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9601 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9661 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9721 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9781 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9841 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9901 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     9961 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10021 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10081 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10141 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10201 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10261 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10321 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10381 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10441 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10501 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10561 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10621 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10681 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10741 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10801 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10861 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10921 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10981 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    11041 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    11101 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    11161 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    11221 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcctttt catcacgtgc
    11281 tataaaaata attataattt aaatttttta atataaatat ataaattaaa aatagaaagt
    11341 aaaaaaagaa attaaagaaa aaatagtttt t
//
