LOCUS       sequence-347039-        9502 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses voltage sensor targeted to dendrites and spines along with
            ChrimsonR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   Cornejo VH, Ofer N, Yuste R
  TITLE     Voltage compartmentalization in dendritic spines in vivo.
  JOURNAL   Science. 2022 Jan 7;375(6576):82-86. doi: 10.1126/science.abg0501. 
            Epub 2021 Nov 11.
  PUBMED    34762487
REFERENCE   2  (bases 1 to 9502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abg0501"; journalName: "Science"; date: "2022-01-7-
            7"; volume: "375"; issue: "6576"; pages: "82-86"
COMMENT     Sequence Label: pCAG-postASAP-p2a-ChrimsonR
FEATURES             Location/Qualifiers
     source          1..9502
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     438..460
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     522..541
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      948..957
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2241..2297
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2304..3350
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=ChrimsonR
                     /note="K176R mutant with accelerated closing kinetics"
                     /translation="AELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTEW
                     WCDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSIA
                     IALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRYF
                     EWLLSCPVILIRLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIVS
                     CIYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLKL
                     SPYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEEF
                     VEEEDEDTV"
     CDS             3363..4073
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     primer_bind     complement(4191..4208)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4197..4421
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4467..4895
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4554..4573)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4764..4785
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4904..4924)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4909..5238
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5089..5224
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5151..5170
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5247..5846
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5247..5266)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5743..5763
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6006..6127
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6043..6062)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6097..6116
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6176..6192)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6176..6192)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6189..6211)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6200..6216
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6224..6230,6231..6248,6249..6254))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6269..6290
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6407..6424)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6578..7163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6658..6677)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7334..8125,8126..8194))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7957..7976
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8195..8299)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8345..8724
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8726..8999
                     /label=chicken beta-actin promoter
ORIGIN
        1 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
       61 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
      121 ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
      181 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
      241 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
      301 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
      361 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg
      421 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
      481 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
      541 gctgtctcat cattttggca aagaattcct cgaggaattc actcctcagg tgcaggctgc
      601 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatcc
      661 ccgggattac aatgctcgaa gtcaaggaag catcaccaac cagcatccag atcagctggg
      721 tgctccactt gcgccacgtt cgctactacc gcatcaccta cggtgaaact ggtggcaata
      781 gccctgtcca ggaattcacc gtgcctggca gcaagtccac tgctaccatc agcggcctga
      841 aacctggtgt cgactatacc atcacggtgt acgccgtcac gatcttcagc gcctaccgct
      901 ccgcctggcc gccgatctcc atcaactacc gcaccggaac cctagccgcc accatggaga
      961 cgactgtgag gtatgaacag gggtcagagc tcactaaaac ttcgagctct ccaacagcag
     1021 atgagcccac gataaagatt gatgatggtc gtgatgaggg taatgaacaa gacagctgtt
     1081 ccaataccat taggagaaaa atttccccgt ttgtgatgtc atttggattc agagtatttg
     1141 gagttgtgct tatcattgta gacatcatag tggtgattgt ggatctggcc atcagtgaga
     1201 agaaaagagg cattagagag attcttgaag gtgtttccct ggctatagca ctcttcttcc
     1261 ttgttgatgt tctcatgaga gtgtttgttg aaggcttcaa gaactatttc cggtccaaac
     1321 tgaatacttt ggatgcagtc atagtagtgg gcactctgct aattaatatg acctactcct
     1381 tctctgacgg tacctttagg ggcgataacg tgtatattac cgcggataaa cagaaaaacg
     1441 gcattaaagc gaactttacc gtgcgccata acgtggaaga tggcagcgtg cagctggcgg
     1501 atcattatca gcagaacacc ccgattggcg atggcccggt gctgctgccg gataaccatt
     1561 atctgagcac ccagaccgtg ctgagcaaag atccgaacga aaaacgcgat cacatggtgc
     1621 tgctggaatt tgtgaccgca gcgggcatta cacacggcat ggatgaactg tatggcggca
     1681 ccggcggcag cgcgagccag ggcgaagaac tgtttaccgg cgtggtgccg attctggtgg
     1741 aactggatgg cgatgtgaac ggccataaat ttagcgtgcg cggcgaaggc gaaggcgatg
     1801 cgaccattgg caaactgacc ctgaaattta tttgcaccac cggcaaacta ccggtgccgt
     1861 ggccgaccct ggtgaccacc ttaacctatg gcgtgcagtg ctttagccgc tatccggatc
     1921 atatgaaacg ccatgatttt tttaaaagcg cgatgccgga aggctatgtg caggaacgca
     1981 ccattagctt taaagatgat ggcaaatata aaacccgcgc ggtggtgaaa tttgaaggcg
     2041 ataccctggt gaaccgcatt gaactgaaag gcaccgattt taaagaagat ggcaacattc
     2101 tggggcataa actggaatat aacacagatc agatgccgca gatggttcgt cttcttcgag
     2161 ttctgagaat tgttatctta ataagaatat ttcgcctggc ttcacagaag aaacaacttg
     2221 aagtggtaac cggctccgga gccacgaact tctctctgtt aaagcaagca ggagacgtgg
     2281 aagaaaaccc cggtcccgtc gacgctgagc tgatcagcag cgccaccaga tctctgtttg
     2341 ccgccggagg catcaaccct tggcctaacc cctaccacca cgaggacatg ggctgtggag
     2401 gaatgacacc tacaggcgag tgcttcagca ccgagtggtg gtgtgaccct tcttacggac
     2461 tgagcgacgc cggatacgga tattgcttcg tggaggccac aggcggctac ctggtcgtgg
     2521 gagtggagaa gaagcaggct tggctgcaca gcagaggcac accaggagaa aagatcggcg
     2581 cccaggtctg ccagtggatt gctttcagca tcgccatcgc cctgctgaca ttctacggct
     2641 tcagcgcctg gaaggccact tgcggttggg aggaggtcta cgtctgttgc gtcgaggtgc
     2701 tgttcgtgac cctggagatc ttcaaggagt tcagcagccc cgccacagtg tacctgtcta
     2761 ccggcaacca cgcctattgc ctgcgctact tcgagtggct gctgtcttgc cccgtgatcc
     2821 tgatcagact gagcaacctg agcggcctga agaacgacta cagcaagcgg accatgggcc
     2881 tgatcgtgtc ttgcgtggga atgatcgtgt tcggcatggc cgcaggactg gctaccgatt
     2941 ggctcaagtg gctgctgtat atcgtgtctt gcatctacgg cggctacatg tacttccagg
     3001 ccgccaagtg ctacgtggaa gccaaccaca gcgtgcctaa aggccattgc cgcatggtcg
     3061 tgaagctgat ggcctacgct tacttcgcct cttggggcag ctacccaatc ctctgggcag
     3121 tgggaccaga aggactgctg aagctgagcc cttacgccaa cagcatcggc cacagcatct
     3181 gcgacatcat cgccaaggag ttttggacct tcctggccca ccacctgagg atcaagatcc
     3241 acgagcacat cctgatccac ggcgacatcc ggaagaccac caagatggag atcggaggcg
     3301 aggaggtgga agtggaagag ttcgtggagg aggaggacga ggacacagtg gcggcaccgg
     3361 taatggtgtc taagggcgaa gagctgatca aggaaaatat gcgtatgaag gtggtcatgg
     3421 aaggttcggt caacggccac caattcaaat gcacaggtga aggagaaggc aatccgtaca
     3481 tgggaactca aaccatgagg atcaaagtca tcgagggagg acccctgcca tttgcctttg
     3541 acattcttgc cacgtcgttc atgtatggca gccgtacttt tatcaagtac ccgaaaggca
     3601 ttcctgattt ctttaaacag tcctttcctg agggttttac ttgggaaaga gttacgagat
     3661 acgaagatgg tggagtcgtc accgtcatgc aggacaccag ccttgaggat ggctgtctcg
     3721 tttaccacgt ccaagtcaga ggggtaaact ttccctccaa tggtcccgtg atgcagaaga
     3781 agaccaaggg ttgggagcct aatacagaga tgatgtatcc agcagatggt ggtctgaggg
     3841 gatacactca tatggcactg aaagttgatg gtggtggcca tctgtcttgc tctttcgtaa
     3901 caacttacag gtcaaaaaag accgtcggga acatcaagat gcccggtatc catgccgttg
     3961 atcaccgcct ggaaaggtta gaggaaagtg acaatgaaat gttcgtagta caacgcgaac
     4021 acgcagttgc caagttcgcc gggcttggtg gtgggatgga cgagctgtac aagtaatgaa
     4081 agcttggtac cgagctcgga tccactagtc cagtgtggtg gaattctgca gatatccagc
     4141 acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     4201 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     4261 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     4321 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     4381 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     4441 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     4501 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4561 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4621 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4681 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4741 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4801 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4861 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     4921 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4981 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5041 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5101 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5161 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5221 ggcctaggct tttgcaaaaa gctcccatga ccgagtacaa gcccacggtg cgcctcgcca
     5281 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     5341 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     5401 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     5461 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     5521 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     5581 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     5641 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     5701 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     5761 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     5821 gcatgacccg caagcccggt gcctgattcg aaatgaccga ccaagcgacg cccaacctgc
     5881 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5941 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     6001 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6061 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6121 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6181 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6241 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6301 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6361 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6421 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6481 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6541 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6601 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6661 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6721 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6781 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6841 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6901 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6961 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7021 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7081 cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta
     7141 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7201 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7261 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7321 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7381 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7441 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7501 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7561 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7621 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7681 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7741 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7801 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7861 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7921 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7981 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8041 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8101 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8161 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8221 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8281 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     8341 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     8401 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     8461 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     8521 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     8581 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     8641 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     8701 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     8761 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     8821 gcgatggggg cggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
     8881 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
     8941 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
     9001 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
     9061 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     9121 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
     9181 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
     9241 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
     9301 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     9361 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     9421 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     9481 cccctccccg agttgctgag ca
//
