LOCUS       sequence-347042-       10728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde 
            transport of recycling endosomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Nijenhuis W, van Grinsven MMP, Kapitein LC
  TITLE     An optimized toolbox for the optogenetic control of intracellular 
            transport.
  JOURNAL   J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 
            10.1083/jcb.201907149.
  PUBMED    32328628
REFERENCE   2  (bases 1 to 10728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-04-6"; volume: "219(4). pii: 133834. doi"; 
            pages: " 10.1083/jcb.201907149"
COMMENT     Sequence Label: 
            pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11
FEATURES             Location/Qualifiers
     source          1..10728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1059..1081
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      1144..1153
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2126..2135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2749..2751,2752..3462)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2749..2751,2752..3462)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2791..2812)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3052..3071)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3399..3420
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             join(3592..3798,3799..3801,3802..3921)
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein and with the mutation R74Q to reduce the 
                     affinity for iLID"
                     /label=SspB micro
                     /note="dimerizes with iLID at moderate affinity to create 
                     iLID micro (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNAQFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     CDS             3934..3990
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3997..4434
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     CDS             4474..5178
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4616..4634)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    6052..6186
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6089..6108)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6143..6162
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6200..6216)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6200..6216)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6213..6235)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6224..6240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6248..6254,6255..6272,6273..6278))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6293..6314
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6431..6448)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6602..7190)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6682..6701)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7361..8152,8153..8221))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7984..8003
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8222..8326)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8394..8412
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8450..8472)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8572..8591
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8785..8807
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8799..8816
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8800..8816
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt
       61 ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag
      121 tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      181 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      241 gctgtaatta gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc
      301 ttaaagggct ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg
      361 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      421 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
      481 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
      541 gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc
      601 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg
      661 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
      721 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
      781 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
      841 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
      901 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
      961 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgtccgca
     1021 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1081 cggcggggtt tatatcttcc cttctctgtt cctccgcagc ccccaagcta ggcaattggg
     1141 cgcgccacca tggccggggc ttcggtgaag gtggcggtgc gtgtccgccc cttcaactcc
     1201 cgggaaatga gccgagactc caagtgcatc attcagatgt ctggaagcac caccactatt
     1261 gtcaacccca aacagcccaa ggagacaccc aagagcttca gctttgatta ctcctactgg
     1321 tcacatacct cgccggagga catcaactat gcctctcaga agcaggtcta cagggacatt
     1381 ggggaagaga tgctacagca tgcctttgag ggctacaatg tgtgcatctt tgcctatggg
     1441 cagacggggg ccggcaagtc ctacaccatg atggggaaac aggagaagga ccagcaaggc
     1501 atcattccac agctctgcga agacctcttc tctcgaatca acgacaccac caacgacaac
     1561 atgtcctact ctgtggaggt cagctacatg gagatttact gtgagcgagt ccgtgacctc
     1621 ctgaacccca agaacaaagg caacctgcgt gtgagggagc accctctgct ggggccttat
     1681 gtggaggacc tctccaagct ggctgtaacc tcctacaatg acatccagga cctcatggac
     1741 tcagggaaca aggccaggac tgtggcggcc acaaacatga atgagaccag cagccgttct
     1801 cacgcagtct tcaacatcat ctttacccag aagcgccatg atgcagagac caacatcacc
     1861 acagagaaag tgagcaaaat cagcctggtg gacctggcag gcagtgaacg agccgactcc
     1921 acaggagcca agggcacgcg actcaaggag ggggccaaca tcaacaagtc tttgaccacc
     1981 ctgggaaagg tcatctctgc tctggctgaa atggactctg gacccaacaa gaacaagaaa
     2041 aagaagaaga cagatttcat cccttaccga gactccgtat tgacatggct tctccgggaa
     2101 aacctcggtg gcaactcaag gacagccatg gtggcagccc tgagccctgc ggacatcaac
     2161 tatgatgaga ccctcagcac actgagatat gcagatcggg ccaaacaaat ccgctgcaat
     2221 gccatcatca atgaagaccc caactctaga ggtggcgggg gcagtggggg atcccatact
     2281 ctttatgccc ccggtggata tgacattatg ggatatctgt gccagatcat gaaccggcca
     2341 aacccgcagg tggaactggg ccccgtggat acatcctgcg ccttggttct ttgtgacctg
     2401 aaacagaaag acaccccggt agtttacgcg agtgaagcct tcctctacat gacaggttac
     2461 agcaacgcag aggtgctggg ccggaattgc cggtttctgc aaagccctga cggcatggtg
     2521 aagcccaaga gcacccggaa gtacgtggat agtaacacaa tcaatactat gcgcaaggca
     2581 atcgacagga atgccgaggt gcaggttgaa gtagtcaatt ttaaaaagaa tggacagcga
     2641 tttgttaatt tcctgactat gatacctgtt agggacgaaa caggcgagta tcgatactct
     2701 atgggattcc agtgcgaagt cgacgggagc gggggtggga gtaagcttgt gagcaagggc
     2761 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     2821 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     2881 aagttgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctc
     2941 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     3001 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3061 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3121 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3181 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggcaaac
     3241 ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag
     3301 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     3361 tccaaactga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3421 accgccgccg ggatcactct cggcatggac gagctgtaca aggctagcgg aggcggtggc
     3481 tcaggcggag gagggtcagg agggggaggc agtggcgggg gcggctctgg aggagggggt
     3541 tctggtggtg gtagctacgt agatctgagc ggcctgaccc tgcaggaatt cagctccccg
     3601 aaacgcccta agctgctgcg tgaatattac gattggctgg ttgataacag ctttacccca
     3661 tatctggtgg tggatgccac atacctgggc gtgaacgtgc ccgtggagta tgtgaaagac
     3721 ggtcagatcg tgctgaatct gtctgcaagt gcgaccggca acctgcaact gacaaatgat
     3781 tttatccagt tcaacgccca gtttaagggc gtgtctcgtg aactgtatat cccgatgggt
     3841 gccgctctgg ccatttacgc tcgcgagaac ggcgatggtg tgatgttcga accagaagaa
     3901 atctatgacg agctgaatat tggtggatcc ggagccacga acttctctct gttaaagcaa
     3961 gcaggagacg tggaagaaaa ccccgggccc accatgctgg caaccacact ggaacggatc
     4021 gagaaaaatt tcgtgattac tgatccgaga ctgcctgaca acccaatcat ttttgcgagc
     4081 gattccttcc tgcagctgac agaatattct cgggaagaga tcctggggcg caattgccgt
     4141 tttctgcagg gacccgagac agaccgtgcc actgttcgga aaatcagaga tgctattgac
     4201 aaccagactg aagtgaccgt tcagctgatc aattatacca agagcggcaa gaagttctgg
     4261 aacgtgttcc acctgcagcc gatgcgcgat tataagggcg acgtccagta cttcattggc
     4321 gtgcagctgg atggcaccga acgtcttcat ggcgccgctg agcgtgaggc ggtctgcctg
     4381 atcaaaaaga cagcctttca gattgctgag gcagcgaacg acgaaaatta cttttctaga
     4441 ggtggcgggg ggagcggggg tgggagtaag cttgtgagca agggcgagga ggataacatg
     4501 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     4561 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     4621 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     4681 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     4741 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     4801 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     4861 ggcaccaact tcccctccga cggtcccgta atgcagaaga agaccatggg ctgggaggcc
     4921 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     4981 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     5041 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     5101 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     5161 atggacgagc tgtacaaggc tagcggaggc ggtggctcag gcggaggagg gtcaggaggg
     5221 ggaggcagtg gcgggggcgg ctctggagga gggggttctg gtggtggtag ctacgtaggc
     5281 acccgcgacg acgagtacga ctatctcttc aaagttgtcc ttattggaga ttctggtgtt
     5341 ggaaagagta atctcttgtc tcgatttact cgaaatgagt ttaatctcga aagtaagagc
     5401 accattggag tagagtttgc aacaagaagc atccaggttg atgggaaaac aataaaggca
     5461 cagatatggg acacggcagg gcaggagcga taccgagcta taacatcagc atattatcgt
     5521 ggagctgtag gtgccttact ggtttatgac attgctaagc atctcacata tgaaaatgta
     5581 gaacgatggc tgaaagaact gagagatcat gctgatagta acattgttat catgcttgtg
     5641 ggcaataaga gtgatttgcg tcatctcagg gcagttccta cagatgaagc aagagctttt
     5701 gcagaaaaga atggtttgtc attcattgag acttccgctc tagactctac aaatgtagaa
     5761 gctgcttttc agacaattct gacagagata taccgcattg tttcccagaa acaaatgtca
     5821 gacagacgtg aaaatgacat gtctccaagc aacaatgtgg ttcctattca tgttccacca
     5881 accactgaaa acaagccaaa ggtgcagtgc tgtcagaaca tctaaaccgg tcctagataa
     5941 gtaatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc
     6001 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     6061 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     6121 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     6181 tggatcaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6241 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6301 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6361 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6421 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6481 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6541 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6601 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6661 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6721 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6781 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6841 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6901 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6961 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7021 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7081 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7141 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7201 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7261 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7321 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7381 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7441 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7501 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7561 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7621 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7681 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7741 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7801 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7861 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7921 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7981 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8041 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8101 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8161 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8221 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8281 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8341 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     8401 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     8461 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     8521 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     8581 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     8641 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     8701 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     8761 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
     8821 agttgggatc tttgcattgg cccacggctc tcaggatggg gatgctcccc ttcagcaccc
     8881 ggttcccctt ggaaactgat ggtcctggct ctgtggcatg gcagtggcac tgtgaggagc
     8941 cccctaccag cagcacacag tggggttggc actgccacgc tccggatgcc gcgctctgat
     9001 ccaaccccat aatcaaggga acccgaattg ccccatcatt gcccccacca cccccatcct
     9061 gccgggccct cacaccccac gctgccttct ggtgacattc cccagcccaa acccacggct
     9121 tcatggctac cgcggggcat ttcccattgc cgccccatta tcagctctgc acacctcccg
     9181 ctgtacccat gcctcgtggc tgcccttctt tgacgtataa tcttctaatt aatacccggc
     9241 cttgtcaaag tggagcacaa acgttaatta attccccagc aggcaggtaa ttaacagtgt
     9301 gactcccttt ttgctgcgag tggggctgat acagagagat gtggcactat ggagcccacg
     9361 gggtcctggc actgggtgcc cacggaggtc cccatgtgct gcagtgtcac cgcctccgag
     9421 gagacagtat tgtccctgcg gtgtccctgc agctcagctc tgtccacagg gccacctcca
     9481 gtttggaggg gacacaatgc agccccgatg caacccatcc tcgcagcatc ccagggacaa
     9541 agaccccact gcaagaccgc acacagggct gggtcccgct cccctaatat ctacagtgct
     9601 tcttgcatgg ccccttaatc aatgcagtta atcagcatgc gctcatgcac cgctctggag
     9661 ctgcaaagcc cctcgcagcg ctgctcacca acaccgcgca ccgccccggc ccagcctgca
     9721 gcacgcgctg caaacaggaa agaaacaaaa tattgcccaa atgtaggcaa aggcattcgg
     9781 ctgccttgac ctccgccggg ccgggccctg cctgactcag ctccttactc agcgctcgct
     9841 tcctccctcc ggctgccacc gccgcagcgc acaccctgac aaagagtggc ccttaacggg
     9901 ctctgaggtg cacccagcag tgcactcagc agtccaaggg ccggcctgga ggtttgcacc
     9961 gctacgtgct gacattagca ttgaacttgg ccctgggtag tgctgcaggc cgggcggggt
    10021 gggtgtagag agtgcagcgc gcgttgcacc cggtgcccct tcccctccct tgcatcccag
    10081 caggctgcac cccagcacca ggcccgtgca tgcatgctcc tggtgttatt gcagcctggt
    10141 gcatgcatgc gtcttgatgg tgcagcgctg tgcatgcatc ctccttggtg tgtagcagtc
    10201 tagtgcatgc atacccctcg gtgttattgc tgctctgtgc acgcacgctc attgtatcac
    10261 ttcatcccag tgcatgcact cacactggag cgattgctgc tcggtgcacg cacactcatt
    10321 gtatcactgc agctcagtgg ctgcacgcac accggtgtta ttgctgctcg gtgcgtgcat
    10381 gcacatcagt gtcgctgcag ctcagtgcat gcacgctcat tgcccatcgc tatccctgcc
    10441 tctcctgctg gcgctccccg ggaggtgact tcaaggggac cgcaggacca cctcgggggt
    10501 ggggggaggg ctgcacacgc ggaccccgct ccccctcccc aacaaagcac tgtggaatca
    10561 aaaagggggg aggggggatg gaggggcgcg tcacaccccc gccccacacc ctcacctcga
    10621 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt
    10681 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggg
//
