LOCUS       sequence-347044-       11514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prgef-1 EGL-3 unc-54 3' UTR C.elegans pan-neural expression of 
            EGL-3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 11514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH2124
FEATURES             Location/Qualifiers
     source          1..11514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8642..8659)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8642..8658)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8651..8673)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8871..9326
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8958..8977)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9168..9189
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9343..9362)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9462..9484
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9522..9540)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9608..9712
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9713..9781,9782..10573)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9931..9950)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10744..11332
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11233..11252
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11486..11503
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgaatg
      241 gccatgggac gtcgacaagc ttgcatgcct gcagcgtttc cgataccccc ttatatcagc
      301 acacattcag tcatcatacc acaaaatgtt gtgatagtga taggcaaaac taccgtattt
      361 cctctattaa tcttgcatgc aagactaatt ttcgattaac ccgtaggggt gcaagactaa
      421 tagagactgc aagactatta gaggctgaaa tactaatttt cgtatgctca ataattttgg
      481 aaattggcct attttttgta gaaacttgat accgtttaaa caaggaaaaa tacacacttt
      541 ttaatatttc atcaataatt tgaacgattt tgtgatttta agttcaattt gcccaaaaaa
      601 agacaatttt tctgacgtta ccgcaaagta atgctggtca ggcaaaaaga gcggtgcaaa
      661 aatataagag actgcaatac taatagagga aatacggtaa ttgaatttta gtgaaacttg
      721 cgacagtttt cctcattttt ttgttattcc ggcatacgag tgtggcatac gagtgaggtc
      781 atcttttgtt tcttccgttt cttcatcgct tttgaaaaaa aatgttgaag aaaacttcgt
      841 agccgatagc cggataatgt ttggcacggc gttccaacat atatcattgg aaatttttta
      901 agatttttgc gaaaaatcac attcttcacg atgagaacac gttattgaag gaatataaat
      961 caagaataac atatagttat atttctctat tactttaacg ttaaatatga gcaaatttga
     1021 gcattttgat tgcgatgaaa agcagaatcg agtcaactga aatccgttca aaaacctaag
     1081 ctcggttcgc ccgaaagtcg atttttccaa tagccgaaca gcactgcttc tttattttcc
     1141 aggtattttt ggggtgtaag aattatatct tctttgtttg ggtatttacg tttccgaatt
     1201 ccctttcttg atctgtcgct atttttcaga aaccagaaca gtttatcctt tttcatgaga
     1261 acctgaatag ctcaaaaacc cgtttaattt cttttcatca tctcatttga aactttccta
     1321 gtcttcttaa tgatttccag gctcctccta catttgtatc tcaagatgca tacactttat
     1381 ttccgctcca attcgaatca tctacttctc ttttttcttc aactttctac ttttttccat
     1441 tcttttcttt caaatgttcg acgtcttcaa gtgactgctc ttcattctcc tcatttttct
     1501 cgaatttcat tcttgtctct tttttgctca aaaatggaaa atgaaagtga cgtgagattt
     1561 ggacggcggg acacgggggc agtagaagca gcaaaaagga gagaaagagg acacaaataa
     1621 gaagaacgaa ttcaaaaata agcggagagg agctatttcc gtcaattcta cctccccaat
     1681 cttcatcaat tcggctcaat tgaatgacgt cacagggaga taaaccgttt ggatgagcgc
     1741 cgtccatcac tgacgccatc ccgtttggga caagaaaaga gaaaaaagag cacaaagttt
     1801 ttggtgacgg atcttgtcaa tcatatgaaa gttgttctga ttgattgtca gtttttttcc
     1861 tacttttttg gattctatcc acttctgaac ttttgacaag tttcaaactt tctgaatcat
     1921 tttctatgca ttttcctgga attcttttta tgtaaaatat gaaatagaat gtttttgaat
     1981 tcaagttctg cttttttctt cttttttgtt ctgttcgggc ttgggtatgc tttttttaaa
     2041 aattattttg cacatcgacc aataagtgcg caacttataa aattaattta tttttgttaa
     2101 tttttgaaat acttgtattg ctttaagtga tctgacctcg ccctgagctt tccacgtagt
     2161 tatcaaatac aaatcctcca agggtaacgt acctatatta ctgatcttta taataacttt
     2221 atcacctgtc cagttccaga ggaattctgt taagcttata ggcacagaag gagtcatttc
     2281 tgctggtttt taatgatcca aatctttatt tcaagtaaaa aactgaacac ttgcgaataa
     2341 aactatcaga ttaaccattc accaaaaatg tgtttgaatc taaaactttc tcagtattcc
     2401 aaatatagaa ataaataacc acgacattgc taaaatctgt ctgaattgtg tactccttac
     2461 cgtgaagtaa taatggatat aatgaatcgt ttgaaatgaa tgatcagcac atttttggtg
     2521 aaagatcaca aataaggaat aagcgacgga aaataaaacg attatttcgg atcaaaaatt
     2581 tgttgaagat catatacact cgagaccaag attattctag acaattttca aattggcttc
     2641 tttgtttgca aatcttcata ataatctgtg aagtttggaa atttgaattt ttaatctttt
     2701 ttcatagatt atagttttta tttctttgca aaactatatt aaaaaccgat gcattgtttt
     2761 agggaaatta atgagccttt tgttcaacac taaaaacaat aaaattaaaa ttttggcttc
     2821 atcatttgac cttttttaag ttcgaaaact tttctcgtat ttctgaaccg ccaatttttt
     2881 cacacatctc tagacttttg gtgcccgttc cagaaagtta agtaattgct attctaagaa
     2941 agttctcaac attgtttttt agttctgatt gaattctgat gttccaggaa tatattttaa
     3001 atttaatatt ctttgcacta tttctatact aactaaataa taaatagtct aagtatgttc
     3061 aatgagaaca aaaaagctct atctatattt ttctatccta tttcatttat atccttttca
     3121 ttttgaactc accagcttct cttcttcttc ttgtccatat acttcttaaa agtctcgaac
     3181 ttctctctct cactctccat caatttcttt atggctaccg cttgcgctct gccgcttccg
     3241 aagaaagaag gcgttggcag agctctcaat tctatttttt ctcgccgtag gctatcgatt
     3301 tctcagcttc tcttctctct tcctcactcc accaccacca cttcgggctt cttcttttct
     3361 tcattttcgt cttcttcttc atcctcttct ttttttttca gttccccctc actcctcccc
     3421 ttatatgcgc gtgcgagagg ggtgcaaaag cagcgcgcat ccgagaattg aaacgagaaa
     3481 acggagacgc agcagcagtt cgtcctcaga aaaatagcca gtaaaaagag aaaaagatag
     3541 agagagacct atcgcatttt attttcaaat tgcaattcct gcaattcatg tgtgcgtgtg
     3601 cccattttca attcttcccg ttatttttca gaccattacc aacgttcttt ctagatcttg
     3661 agacaatctt ccttctgctc aatcgttcgt cgtgaagacg gcatcgacga cgacgacgat
     3721 cagcagtaaa ggatcccccg ggctgcagga attcgatatg cagaagctga tctcagagga
     3781 ggacctgctt cagaagctta tctcagagga ggaccttcga cctcgagggg gggcccggta
     3841 ccaatgaaaa acacacatgt cgacctaata tgtgtgttcc tgtcgatttt catcggcatt
     3901 ggtgaggcgg tcgacgtcta caccaaccat ttccatgttc atttaaaaga gggaggtgga
     3961 ctggaagatg cgcatcggat agccaaacgt cacggattta ttaatagagg acaagttgca
     4021 gcaagtgata atgaatatca ttttgtgcaa ccagcacttg ttcatgcacg aaccagaaga
     4081 tcagcaggtc atcatgctaa acttcataat gatgatgagg taagaaatca tgggttactg
     4141 tagacttgtt gctgttttga ccaagaaaaa tgttatagag aagcatataa attggtaatc
     4201 agactacttt aaaaaagtaa aagttcggtt agacagttga aaattacatt ttacaactta
     4261 tgataaacat cgctttattt aacagaagtt cttaattcaa attgaggcaa aaacaaaaaa
     4321 ctaaatgatg tgaatttcat agaatttagt gcggcacatt tttgccaaaa ttttaaaagt
     4381 tatattaact aggcgatttg cagtcattaa ttgaatactg acactgacat tgaatggaat
     4441 ataattcgta ttatttcggt aattactgat cgcagtattt attatcatga acaagtgctg
     4501 gttgcacaaa tatttaaact agaaacatta tactacacag ttctgttcaa aagcattcgt
     4561 caccacataa tgtgaaattt gtaaaaaaaa caatcatgac aaattcaaca atgatccgga
     4621 caaattttaa ctttcaaaaa caaaatatat tgatatacta atcccataat tctcttcacc
     4681 atacacctat ttttacaaca ttgatcagct aaatctgtgt taaatccaat tcaaaatggt
     4741 cattcatttg atcatttaga gcaatagaat attccttcca acatctcttc cctacgtcat
     4801 ttcatccaac ttttttcatt ctttattcag gttcttcacg tcgagcagct gaaaggatac
     4861 acccgtacaa agcgaggata tcgtccgctt gaacagcgac tggaaagcca gtttgacttt
     4921 tcagcagtca tgtctccatc ggatccactt tatggatatc agtggtactt ggtgagtcaa
     4981 ggaacacatt ttgaacagaa ataatgttac ttggtatttc gaattcaggc aaccacgtga
     5041 cgcaattcgt tcagttcgta ctaatttcct agtatttcag atagtatgca ttagataatg
     5101 agaataacaa aaatatttcc gtcttcaacg ttttttttaa taaatcgtaa ttccagaact
     5161 aaggacacga aaacattgat aacttttatt aattaaacct gcacaaaagt ttttttgatg
     5221 tttttctgat cgtgcacaac gtctaccctc tccccccata aattagggat ccattttctt
     5281 ggttgtccct accctgaggg cttcatcaga atttcctttc gtctcgtctt tccgttgctt
     5341 tcctcccaac catgtaaata gttcatgaga taacttcgtc atgaaaagaa aaaaacgagg
     5401 gaggagaata aagaaaaatg cgccccaatg ttctgctgct ctgcgccaaa aaattctctc
     5461 cgaaacatgc tctttttcca gaaaaacact ggacaagctg gcggaaaagc tcgtttggat
     5521 ttgaatgtgg aaagggtaac aaattgaaaa gaaaaataaa aattttggaa aataaaaatt
     5581 ttaaggcttg ggcaatggga tttactggaa agaacatcac aacagcgatt atggatgacg
     5641 gagtggatta catgcatccg gatattaaga acaactttgt aagtcgtctg attacacatt
     5701 acaatcaggg tcttatcgtt ttggtagaaa ctcacatatt ttgatatttg agacaaatag
     5761 tgaagccata aaatagggaa actgaattaa attccaaagc tgtcttgaga acactaaaat
     5821 gcccagtaaa tttttttgga tttctcttcg aagaaatcag aaaccaaaaa tgtaaagtct
     5881 agaataacca aaaagctatt gaaaacttac ttgagagcca tttgtctcaa aaaatggatt
     5941 ctcaaaagtc tcatttgtag acgtaacata attgaacatt tatttcactg atttttataa
     6001 tctttttaaa attgatgttt aaaattgaaa atttcgtcaa attatctgtt tacagtagat
     6061 ttatgaatcg aatttttttc cagaatgccg aagcttcata cgatttctca tcaaatgatc
     6121 cattcccata tccaagatac actgatgact ggttcaattc ccacggaact cgatgcgccg
     6181 gagaaatcgt tgccgcccgt gacaacggtg tctgtggagt cggagttgcc tatgacggaa
     6241 aggttgctgg aatcagaatg cttgatcaac cctacatgac cgatctcatt gaagccaatt
     6301 caatgggaca cgagccgagc aaaattcata tctactctgc ttcatgggga ccgactgatg
     6361 atggaaagac tgttgatgga ccacgtaatg ccactatgcg agcaatcgtc aggggtgtca
     6421 acgagggacg taacggactt ggatccattt ttgtttgggc cagtggagac ggaggagagg
     6481 atgatgattg taattgtgat ggatatgcgg caagcatgtg gacaatctcg atcaattcag
     6541 ccattaacaa tggagaaaat gctcactacg atgagtatgt ttcgagtttt attttcactt
     6601 caaacacaaa ttaaaacaaa cttcagatct tgttcatcaa cccttgcttc cactttctcc
     6661 aatggaggac gaaacccaga aaccggagtc gctactaccg atctctacgg aagatgcacc
     6721 cgatctcact ccggaacttc cgctgctgct ccagaagcgg caggagtctt tgcccttgct
     6781 cttgaagcca atccatctct tacctggaga gatctccaac acttgacagt tctcacttca
     6841 agcagaaact ccctgtttga cggaagatgc cgtgacttcc catctctcgg aatcaacgat
     6901 aaccaccgcg actctcatgg aaactgttct cactttgaat ggcaaatgaa tggagttgga
     6961 ctcgagtaca atcacctttt cggatttgga gttcttgatg cagcagagat ggtcatgttg
     7021 gcaatggcct ggaagacttc cccaccacgt tatcactgta ctgctggact cattgacact
     7081 ccacagtaag tcacttttac agaatgacca ttcaaattcc taactaatca ttacgttaaa
     7141 ttgctttcag tgagatccca gccgatggaa acttgattct tgaaattaat actgatggat
     7201 gtgccggatc tcaattcgaa gtccgctacc tggaacatgt tcaagcagtt gtctcgttca
     7261 actcgactcg tcgtggagat accactctct acttgatctc tccaatggga acccgtacca
     7321 tgattctttc ccgcagacca aaggatgatg attcaaagga tggattcacc aactggccat
     7381 tcatgacaac acacacatgg ggagagaatc caacaggaaa atggagactt gtcgccagat
     7441 tccaagtgag taatgcattc atgatgaaca attttatttg agcacttaaa tattgtatac
     7501 ctttttctaa gttcgcctaa cgttttttta gaactagatc gaaatcggct gcgaaatcgg
     7561 ctaagataag atatcttaaa atgttgtttt ctcatagatc aaagcacaac atttatatga
     7621 taatctgtac acaaaatttt ctgtaaacac taataatgtt tcagggacct ggagctcatg
     7681 ccggaaccct taagaagttc gagctgatgt tgcacggaac aagagaagcc ccatacaatc
     7741 tcatcgagcc aatcgtcggt caaaccaaca agaagctcga caccgttcaa aaagcccaca
     7801 aacgcagcca cgcggtacca tggtattgat atctgagctc cgcatcggcc gctgtcatca
     7861 gatcgccatc tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta
     7921 catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aaacttcttc
     7981 ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt
     8041 caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta
     8101 ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat gtttttttta
     8161 cttctgataa attttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat
     8221 atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg
     8281 acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag
     8341 tgaaagttta tgaaattaat tttcctgctt ttgctttttg ggggtttccc ctattgtttg
     8401 tcaagagttt cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg
     8461 caagaaagat cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat
     8521 aatttgaaag tggagtagtg tctatggggt ttttgcctta aatgacagaa tacattccca
     8581 atataccaaa cataactgtt tcctactagt ggccacgtgg gccgtgcacc ttaagcttgg
     8641 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     8701 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     8761 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     8821 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     8881 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8941 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     9001 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     9061 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     9121 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     9181 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     9241 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     9301 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     9361 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     9421 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     9481 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     9541 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     9601 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9661 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9721 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9781 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9841 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     9901 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     9961 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    10021 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    10081 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    10141 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    10201 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    10261 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    10321 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    10381 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    10441 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    10501 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    10561 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    10621 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    10681 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10741 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    10801 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    10861 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    10921 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    10981 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    11041 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    11101 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    11161 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    11221 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    11281 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    11341 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    11401 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    11461 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aaga
//
