LOCUS       sequence-347047-        9120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to 
            induce retrograde transport of recycling endosomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Nijenhuis W, van Grinsven MMP, Kapitein LC
  TITLE     An optimized toolbox for the optogenetic control of intracellular 
            transport.
  JOURNAL   J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 
            10.1083/jcb.201907149.
  PUBMED    32328628
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-04-6"; volume: "219(4). pii: 133834. doi"; 
            pages: " 10.1083/jcb.201907149"
COMMENT     Sequence Label: 
            pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-R
            AB11
FEATURES             Location/Qualifiers
     source          1..9120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(19..21,22..732)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(19..21,22..732)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(61..82)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(322..341)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     669..690
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    742..766
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             1230..1238
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             2317..2373
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2380..2817
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     CDS             2857..3561
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(2999..3017)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    4435..4569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4472..4491)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4526..4545
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4583..4599)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4583..4599)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4596..4618)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4607..4623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4631..4637,4638..4655,4656..4661))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4676..4697
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4814..4831)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4985..5573)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5065..5084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5744..6535,6536..6604))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6367..6386
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6605..6709)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6777..6795
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6833..6855)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6955..6974
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7168..7190
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7182..7199
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7183..7199
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggggtggga gtaagcttgt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
       61 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
      121 ggcgatgcca cctacggcaa gctgaccctg aagttgatct gcaccaccgg caagctgccc
      181 gtgccctggc ccaccctcgt gaccaccctc ggctacggcc tgcagtgctt cgcccgctac
      241 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
      301 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
      361 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
      421 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
      481 gacaagcaga agaacggcat caaggcaaac ttcaagatcc gccacaacat cgaggacggc
      541 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
      601 ctgcccgaca accactacct gagctaccag tccaaactga gcaaagaccc caacgagaag
      661 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      721 gagctgtaca agcaccggta tacaagtttg tacaaaaaag caggctccgc tattgaagac
      781 aagttggaag aaatactttc gaaattgtac cacattgaaa atgagttggc cagaattaag
      841 aaattattgg gcgaaattgg tggaggtagt ggtggaggta gtgcggccgc cagagatgat
      901 ttgaggggac gacttgtaac tctcgagaag aaattagcag agcgtaccca agatttgagt
      961 cttgcagagt ctactttggc aactcgtgag ggtgaattcg aatccctgca aagcaatcta
     1021 atggagctta atgagctccg ggagatgaag gaggatatcg acagaaagaa tgagcaaaca
     1081 gcagctatat tgaagcgaca agcagaccag ataactgaat tagaaactct ttatagggaa
     1141 gaacaagtcc ttcgaaaacg atacttcaac atgatggaag acatgaaagg taagatcagg
     1201 gtatatgctc ggtggagacc cctaagtagt aaggaggtca aagagcggca acaaaacgtg
     1261 ttgatagcac ctgatgaatt caccatcgaa catccttgga aagacgacaa acccaaacaa
     1321 catcaatttg atcacgtttt cgaccaccat gcgacccaag aagaggtctt tgaggataca
     1381 aagtatttgg tccaatcagc aattgacgga tacaatgtct gtatatttgc atacgggcaa
     1441 actgggtcag gcaaaacgtt tacgatttac ggttctgaca acaacccagg tctaactcca
     1501 cgagctacca aagaactttt tggctatctt aagcgagacg ccaataagtt ttcgtttgct
     1561 ctgaaggttt acatgcttga aatatatcaa gattcgctca tcgatctgct tctaccaaag
     1621 agtgctgcaa agccacgaaa gcttgagatt aagaaggact ccaagggaat ggttgtcgta
     1681 gagaatgcaa ctctactgcc gattgcatcc catgatgaac tgcaagccat tgttcataag
     1741 ggtctggaga gacgacatgt ttcgggcacc cacatgaatg cggagagttc acggtcacac
     1801 ctcattctat cagtaatcgt tgaaagcacc aaccgacagt ctcaagttct tgtgaagggc
     1861 aagctgagtt ttgtggatct tgcgggctct gaaagagtga agaaatctgg atccagtggt
     1921 gaacaactga aagaggccca aagcattaat aaatctttgt cagcactagg tgatgtcatt
     1981 agcgcccttg caacagagga gcagcatatt ccttaccgta accacaagct aacaatgctt
     2041 atgagcgatt ctctcggtgg aaatgcgaag accctcatgt ttgtaaatat ctcaccagca
     2101 gagtctaatc tggacgagac ccataattca ctctgctatg ccacaagggt aagatccatc
     2161 atcaatgagg ccagtaaaaa tactacaacg aaagagatac tgcgactgaa gaaacaaatc
     2221 gcgtactgga aggagcaagc gggcaggatg ggagggtccg aggatctgga agaggttatg
     2281 gatgatcgtg gaatcaaaac tgcagatgga tccggagcca cgaacttctc tctgttaaag
     2341 caagcaggag acgtggaaga aaaccccggg cccaccatgc tggcaaccac actggaacgg
     2401 atcgagaaaa atttcgtgat tactgatccg agactgcctg acaacccaat catttttgcg
     2461 agcgattcct tcctgcagct gacagaatat tctcgggaag agatcctggg gcgcaattgc
     2521 cgttttctgc agggacccga gacagaccgt gccactgttc ggaaaatcag agatgctatt
     2581 gacaaccaga ctgaagtgac cgttcagctg atcaattata ccaagagcgg caagaagttc
     2641 tggaacgtgt tccacctgca gccgatgcgc gattataagg gcgacgtcca gtacttcatt
     2701 ggcgtgcagc tggatggcac cgaacgtctt catggcgccg ctgagcgtga ggcggtctgc
     2761 ctgatcaaaa agacagcctt tcagattgct gaggcagcga acgacgaaaa ttacttttct
     2821 agaggtggcg gggggagcgg gggtgggagt aagcttgtga gcaagggcga ggaggataac
     2881 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     2941 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     3001 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     3061 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     3121 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     3181 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     3241 cgcggcacca acttcccctc cgacggtccc gtaatgcaga agaagaccat gggctgggag
     3301 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     3361 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     3421 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     3481 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     3541 ggcatggacg agctgtacaa ggctagcgga ggcggtggct caggcggagg agggtcagga
     3601 gggggaggca gtggcggggg cggctctgga ggagggggtt ctggtggtgg tagctacgta
     3661 ggcacccgcg acgacgagta cgactatctc ttcaaagttg tccttattgg agattctggt
     3721 gttggaaaga gtaatctctt gtctcgattt actcgaaatg agtttaatct cgaaagtaag
     3781 agcaccattg gagtagagtt tgcaacaaga agcatccagg ttgatgggaa aacaataaag
     3841 gcacagatat gggacacggc agggcaggag cgataccgag ctataacatc agcatattat
     3901 cgtggagctg taggtgcctt actggtttat gacattgcta agcatctcac atatgaaaat
     3961 gtagaacgat ggctgaaaga actgagagat catgctgata gtaacattgt tatcatgctt
     4021 gtgggcaata agagtgattt gcgtcatctc agggcagttc ctacagatga agcaagagct
     4081 tttgcagaaa agaatggttt gtcattcatt gagacttccg ctctagactc tacaaatgta
     4141 gaagctgctt ttcagacaat tctgacagag atataccgca ttgtttccca gaaacaaatg
     4201 tcagacagac gtgaaaatga catgtctcca agcaacaatg tggttcctat tcatgttcca
     4261 ccaaccactg aaaacaagcc aaaggtgcag tgctgtcaga acatctaaac cggtcctaga
     4321 taagtaatga tcataatcag ccatatcaca tctgtagagg ttttacttgc tttaaaaaac
     4381 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     4441 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4501 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4561 gtctggatca attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4621 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     4681 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     4741 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4801 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4861 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4921 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4981 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5041 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5101 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5161 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5221 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5281 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5341 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5401 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5461 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5521 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5581 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5641 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5701 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5761 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5821 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5881 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5941 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6001 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6061 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6121 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6181 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6241 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6301 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6361 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6421 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6481 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6541 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6601 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6661 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6721 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     6781 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     6841 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     6901 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     6961 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     7021 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     7081 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     7141 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     7201 ccaagttggg atctttgcat tggcccacgg ctctcaggat ggggatgctc cccttcagca
     7261 cccggttccc cttggaaact gatggtcctg gctctgtggc atggcagtgg cactgtgagg
     7321 agccccctac cagcagcaca cagtggggtt ggcactgcca cgctccggat gccgcgctct
     7381 gatccaaccc cataatcaag ggaacccgaa ttgccccatc attgccccca ccacccccat
     7441 cctgccgggc cctcacaccc cacgctgcct tctggtgaca ttccccagcc caaacccacg
     7501 gcttcatggc taccgcgggg catttcccat tgccgcccca ttatcagctc tgcacacctc
     7561 ccgctgtacc catgcctcgt ggctgccctt ctttgacgta taatcttcta attaataccc
     7621 ggccttgtca aagtggagca caaacgttaa ttaattcccc agcaggcagg taattaacag
     7681 tgtgactccc tttttgctgc gagtggggct gatacagaga gatgtggcac tatggagccc
     7741 acggggtcct ggcactgggt gcccacggag gtccccatgt gctgcagtgt caccgcctcc
     7801 gaggagacag tattgtccct gcggtgtccc tgcagctcag ctctgtccac agggccacct
     7861 ccagtttgga ggggacacaa tgcagccccg atgcaaccca tcctcgcagc atcccaggga
     7921 caaagacccc actgcaagac cgcacacagg gctgggtccc gctcccctaa tatctacagt
     7981 gcttcttgca tggcccctta atcaatgcag ttaatcagca tgcgctcatg caccgctctg
     8041 gagctgcaaa gcccctcgca gcgctgctca ccaacaccgc gcaccgcccc ggcccagcct
     8101 gcagcacgcg ctgcaaacag gaaagaaaca aaatattgcc caaatgtagg caaaggcatt
     8161 cggctgcctt gacctccgcc gggccgggcc ctgcctgact cagctcctta ctcagcgctc
     8221 gcttcctccc tccggctgcc accgccgcag cgcacaccct gacaaagagt ggcccttaac
     8281 gggctctgag gtgcacccag cagtgcactc agcagtccaa gggccggcct ggaggtttgc
     8341 accgctacgt gctgacatta gcattgaact tggccctggg tagtgctgca ggccgggcgg
     8401 ggtgggtgta gagagtgcag cgcgcgttgc acccggtgcc ccttcccctc ccttgcatcc
     8461 cagcaggctg caccccagca ccaggcccgt gcatgcatgc tcctggtgtt attgcagcct
     8521 ggtgcatgca tgcgtcttga tggtgcagcg ctgtgcatgc atcctccttg gtgtgtagca
     8581 gtctagtgca tgcatacccc tcggtgttat tgctgctctg tgcacgcacg ctcattgtat
     8641 cacttcatcc cagtgcatgc actcacactg gagcgattgc tgctcggtgc acgcacactc
     8701 attgtatcac tgcagctcag tggctgcacg cacaccggtg ttattgctgc tcggtgcgtg
     8761 catgcacatc agtgtcgctg cagctcagtg catgcacgct cattgcccat cgctatccct
     8821 gcctctcctg ctggcgctcc ccgggaggtg acttcaaggg gaccgcagga ccacctcggg
     8881 ggtgggggga gggctgcaca cgcggacccc gctccccctc cccaacaaag cactgtggaa
     8941 tcaaaaaggg gggagggggg atggaggggc gcgtcacacc cccgccccac accctcacct
     9001 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     9061 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggc
//
