LOCUS       sequence-347048-        7950 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rapalog-inducible coupling to dynein adaptor BICD2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Nijenhuis W, van Grinsven MMP, Kapitein LC
  TITLE     An optimized toolbox for the optogenetic control of intracellular 
            transport.
  JOURNAL   J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 
            10.1083/jcb.201907149.
  PUBMED    32328628
REFERENCE   2  (bases 1 to 7950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-04-6"; volume: "219(4). pii: 133834. doi"; 
            pages: " 10.1083/jcb.201907149"
COMMENT     Sequence Label: HA-BICD2(1-500)-FRB
FEATURES             Location/Qualifiers
     source          1..7950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1076..1098
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1168..1194
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2719..2997
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     polyA_signal    3275..3409
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3312..3331)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3366..3385
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3423..3439)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3423..3439)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3436..3458)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3447..3463
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3471..3477,3478..3495,3496..3501))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3516..3537
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3654..3671)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3825..4413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3905..3924)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4584..5375,5376..5444))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5207..5226
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5445..5549)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5617..5635
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5673..5695)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5795..5814
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6008..6030
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6022..6039
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6023..6039
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc
       61 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
      121 cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc
      181 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
      241 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggctcg tttcttttct
      301 gtggctgcgt gaaagcctta aagggctccg ggagggccct ttgtgcgggg gggagcggct
      361 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
      421 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
      481 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
      541 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcggcg gtcgggctgt
      601 aacccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
      661 ctccgtgcgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
      721 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc
      781 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
      841 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
      901 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
      961 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag
     1021 cctcggggct gtccgcaggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     1081 ggcttctggc gtgtgaccgg cggggtttat atcttccctt ctctgttcct ccgcagcccc
     1141 caagctagcg ctaagcttgc caccatgtac ccatacgacg tcccagacta cgctggcgcg
     1201 cctatgtccg cgccgtcgga ggaggaggag tatgcgcgtc tggtgatgga ggcgcagccg
     1261 gagtggctgc gcgccgaggt gaagcggctg tcccacgagc tggccgagac cacgcgtgag
     1321 aagatccagg cggccgagta tggactggcg gtgctggagg agaagcacca gctcaagctg
     1381 cagttcgagg agctcgaggt ggactatgag gccatccgca gcgagatgga gcagctcaaa
     1441 gaggcatttg gccaggcaca tacaaaccac aagaaggtgg ctgctgatgg tgagagccgg
     1501 gaggagagcc tgatccagga gtcggcctcc aaggagcagt actacgtgcg gaaggtgctg
     1561 gagctgcaga cagagctgaa acagctgcgc aacgtcctca ccaacaccca gtctgagaat
     1621 gagcgcctca cgtctgtggc ccaggagcta aaagagatca accagaatgt ggagatccag
     1681 cgtggtcgcc tgcgggatga catcaaggag tacaagttcc gggaggcccg tctacttcag
     1741 gactactcag agctggagga ggagaacatc agcctgcaga aacaagtgtc tgtgctcagg
     1801 cagaaccagg tggagtttga gggcctcaag catgaaatcg agcgcctgga ggaggagaca
     1861 gagtacctca acagccagct agaggatgcc atccggctta aggagatctc tgaacggcag
     1921 ttggaggagg cgctggagac actgaagaca gagcgggagc agaagaacaa cctgcgcaag
     1981 gagttgtcgc actacatgag catcaacgat tccttctata ccagccacct gcaggtctcc
     2041 ttagatggcc tcaagttcag tgatgatact gtcaccgcag agcccaacaa tgacgccgaa
     2101 gccctggtca atggctttga gcacagtggc ttggtcaaat catccttgga caacaagaca
     2161 tccacaccca ggaaggatgg cctagctcca ccctccccca gcctcgtctc tgacctgctc
     2221 agtgagctcc acatatctga gatccagaag ctgaaacagc agctggtgca gatggagcgg
     2281 gagaaggtgg gcctgctggc aacactgcag gacacacaga agcagctgga gcaggctcgg
     2341 ggaaccctct ctgagcagca tgagaaggtg aatcgtctca cagagaacct tagtgccctc
     2401 cggcgcctgc aggctggcaa ggagcgacag acctcgttgg ataatgagaa ggaccgtgac
     2461 agccacgaag acggtgacta ctatgaggtg gacatcaatg ggcctgagat cctggcctgc
     2521 aagtaccacg tggctgtggc tgaggctggc gagctccggg agcagctcaa ggcgttgcgc
     2581 agcacacacg aagctcgcga agcccagcac gcagaagaaa agggccggta tgaggctgag
     2641 ggccaggcac tcactgagaa gatctctctg ctggagaagg ctagccacca ggaccgagag
     2701 ctggaattcg cttctagaat cctctggcat gagatgtggc atgaaggcct ggaagaggca
     2761 tctcgtttgt actttgggga aaggaacgtg aaaggcatgt ttgaggtgct ggagcccttg
     2821 catgctatga tggaacgggg cccccagact ctgaaggaaa catcctttaa tcaggcctat
     2881 ggtcgagatt taatggaggc ccaagagtgg tgcaggaagt acatgaaatc agggaatgtc
     2941 aaggacctcc tccaagcctg ggacctctat tatcatgtgt tccgacgaat ctcaaagact
     3001 agttaagtcg actagtactt ctcgagctct gtacatgtcc gcggtcgcga cgtacgcgta
     3061 tcgatggcgc cagctgcagg cggccgccat atgcatccta ggcctattaa tattccggag
     3121 tatacgtagc cggctaacgt taacaaccgg tacctctaga taagtaatga tcataatcag
     3181 ccatatcaca tctgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     3241 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     3301 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3361 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca attcgtaatc
     3421 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     3481 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     3541 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     3601 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     3661 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3721 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3781 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3841 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3901 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3961 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4021 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4081 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4141 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4201 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4261 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4321 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4381 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4441 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4501 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4561 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4621 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4681 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4741 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4801 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4861 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4921 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4981 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5041 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5101 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5161 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5221 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5281 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5341 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5401 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5461 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5521 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     5581 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     5641 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     5701 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     5761 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     5821 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     5881 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     5941 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     6001 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagttggg atctttgcat
     6061 tggcccacgg ctctcaggat ggggatgctc cccttcagca cccggttccc cttggaaact
     6121 gatggtcctg gctctgtggc atggcagtgg cactgtgagg agccccctac cagcagcaca
     6181 cagtggggtt ggcactgcca cgctccggat gccgcgctct gatccaaccc cataatcaag
     6241 ggaacccgaa ttgccccatc attgccccca ccacccccat cctgccgggc cctcacaccc
     6301 cacgctgcct tctggtgaca ttccccagcc caaacccacg gcttcatggc taccgcgggg
     6361 catttcccat tgccgcccca ttatcagctc tgcacacctc ccgctgtacc catgcctcgt
     6421 ggctgccctt ctttgacgta taatcttcta attaataccc ggccttgtca aagtggagca
     6481 caaacgttaa ttaattcccc agcaggcagg taattaacag tgtgactccc tttttgctgc
     6541 gagtggggct gatacagaga gatgtggcac tatggagccc acggggtcct ggcactgggt
     6601 gcccacggag gtccccatgt gctgcagtgt caccgcctcc gaggagacag tattgtccct
     6661 gcggtgtccc tgcagctcag ctctgtccac agggccacct ccagtttgga ggggacacaa
     6721 tgcagccccg atgcaaccca tcctcgcagc atcccaggga caaagacccc actgcaagac
     6781 cgcacacagg gctgggtccc gctcccctaa tatctacagt gcttcttgca tggcccctta
     6841 atcaatgcag ttaatcagca tgcgctcatg caccgctctg gagctgcaaa gcccctcgca
     6901 gcgctgctca ccaacaccgc gcaccgcccc ggcccagcct gcagcacgcg ctgcaaacag
     6961 gaaagaaaca aaatattgcc caaatgtagg caaaggcatt cggctgcctt gacctccgcc
     7021 gggccgggcc ctgcctgact cagctcctta ctcagcgctc gcttcctccc tccggctgcc
     7081 accgccgcag cgcacaccct gacaaagagt ggcccttaac gggctctgag gtgcacccag
     7141 cagtgcactc agcagtccaa gggccggcct ggaggtttgc accgctacgt gctgacatta
     7201 gcattgaact tggccctggg tagtgctgca ggccgggcgg ggtgggtgta gagagtgcag
     7261 cgcgcgttgc acccggtgcc ccttcccctc ccttgcatcc cagcaggctg caccccagca
     7321 ccaggcccgt gcatgcatgc tcctggtgtt attgcagcct ggtgcatgca tgcgtcttga
     7381 tggtgcagcg ctgtgcatgc atcctccttg gtgtgtagca gtctagtgca tgcatacccc
     7441 tcggtgttat tgctgctctg tgcacgcacg ctcattgtat cacttcatcc cagtgcatgc
     7501 actcacactg gagcgattgc tgctcggtgc acgcacactc attgtatcac tgcagctcag
     7561 tggctgcacg cacaccggtg ttattgctgc tcggtgcgtg catgcacatc agtgtcgctg
     7621 cagctcagtg catgcacgct cattgcccat cgctatccct gcctctcctg ctggcgctcc
     7681 ccgggaggtg acttcaaggg gaccgcagga ccacctcggg ggtgggggga gggctgcaca
     7741 cgcggacccc gctccccctc cccaacaaag cactgtggaa tcaaaaaggg gggagggggg
     7801 atggaggggc gcgtcacacc cccgccccac accctcacct cgaggtgagc cccacgttct
     7861 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
     7921 aattattttg tgcagcgatg ggggcggggg
//
