LOCUS       sequence-347049-       10202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pgref-1KPC-1 mini-gene unc-54 3' UTR C.elegans neuron and tissue 
            expression of KPC-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH3113
FEATURES             Location/Qualifiers
     source          1..10202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7330..7347)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7330..7346)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7339..7361)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7559..8014
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7646..7665)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7856..7877
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8031..8050)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8150..8172
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8210..8228)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8296..8400
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8401..8469,8470..9261)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8619..8638)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9432..10020
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9921..9940
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10174..10191
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgaatg
      241 gccatgggac gtcgacagct tgcatgcctg cagcgtttcc gataccccct tatatcagca
      301 cacattcagt catcatacca caaaatgttg tgatagtgat aggcaaaact accgtatttc
      361 ctctattaat cttgcatgca agactaattt tcgattaacc cgtaggggtg caagactaat
      421 agagactgca agactattag aggctgaaat actaattttc gtatgctcaa taattttgga
      481 aattggccta ttttttgtag aaacttgata ccgtttaaac aaggaaaaat acacactttt
      541 taatatttca tcaataattt gaacgatttt gtgattttaa gttcaatttg cccaaaaaaa
      601 gacaattttt ctgacgttac cgcaaagtaa tgctggtcag gcaaaaagag cggtgcaaaa
      661 atataagaga ctgcaatact aatagaggaa atacggtaat tgaattttag tgaaacttgc
      721 gacagttttc ctcatttttt tgttattccg gcatacgagt gtggcatacg agtgaggtca
      781 tcttttgttt cttccgtttc ttcatcgctt ttgaaaaaaa atgttgaaga aaacttcgta
      841 gccgatagcc ggataatgtt tggcacggcg ttccaacata tatcattgga aattttttaa
      901 gatttttgcg aaaaatcaca ttcttcacga tgagaacacg ttattgaagg aatataaatc
      961 aagaataaca tatagttata tttctctatt actttaacgt taaatatgag caaatttgag
     1021 cattttgatt gcgatgaaaa gcagaatcga gtcaactgaa atccgttcaa aaacctaagc
     1081 tcggttcgcc cgaaagtcga tttttccaat agccgaacag cactgcttct ttattttcca
     1141 ggtatttttg gggtgtaaga attatatctt ctttgtttgg gtatttacgt ttccgaattc
     1201 cctttcttga tctgtcgcta tttttcagaa accagaacag tttatccttt ttcatgagaa
     1261 cctgaatagc tcaaaaaccc gtttaatttc ttttcatcat ctcatttgaa actttcctag
     1321 tcttcttaat gatttccagg ctcctcctac atttgtatct caagatgcat acactttatt
     1381 tccgctccaa ttcgaatcat ctacttctct tttttcttca actttctact tttttccatt
     1441 cttttctttc aaatgttcga cgtcttcaag tgactgctct tcattctcct catttttctc
     1501 gaatttcatt cttgtctctt ttttgctcaa aaatggaaaa tgaaagtgac gtgagatttg
     1561 gacggcggga cacgggggca gtagaagcag caaaaaggag agaaagagga cacaaataag
     1621 aagaacgaat tcaaaaataa gcggagagga gctatttccg tcaattctac ctccccaatc
     1681 ttcatcaatt cggctcaatt gaatgacgtc acagggagat aaaccgtttg gatgagcgcc
     1741 gtccatcact gacgccatcc cgtttgggac aagaaaagag aaaaaagagc acaaagtttt
     1801 tggtgacgga tcttgtcaat catatgaaag ttgttctgat tgattgtcag tttttttcct
     1861 acttttttgg attctatcca cttctgaact tttgacaagt ttcaaacttt ctgaatcatt
     1921 ttctatgcat tttcctggaa ttctttttat gtaaaatatg aaatagaatg tttttgaatt
     1981 caagttctgc ttttttcttc ttttttgttc tgttcgggct tgggtatgct ttttttaaaa
     2041 attattttgc acatcgacca ataagtgcgc aacttataaa attaatttat ttttgttaat
     2101 ttttgaaata cttgtattgc tttaagtgat ctgacctcgc cctgagcttt ccacgtagtt
     2161 atcaaataca aatcctccaa gggtaacgta cctatattac tgatctttat aataacttta
     2221 tcacctgtcc agttccagag gaattctgtt aagcttatag gcacagaagg agtcatttct
     2281 gctggttttt aatgatccaa atctttattt caagtaaaaa actgaacact tgcgaataaa
     2341 actatcagat taaccattca ccaaaaatgt gtttgaatct aaaactttct cagtattcca
     2401 aatatagaaa taaataacca cgacattgct aaaatctgtc tgaattgtgt actccttacc
     2461 gtgaagtaat aatggatata atgaatcgtt tgaaatgaat gatcagcaca tttttggtga
     2521 aagatcacaa ataaggaata agcgacggaa aataaaacga ttatttcgga tcaaaaattt
     2581 gttgaagatc atatacactc gagaccaaga ttattctaga caattttcaa attggcttct
     2641 ttgtttgcaa atcttcataa taatctgtga agtttggaaa tttgaatttt taatcttttt
     2701 tcatagatta tagtttttat ttctttgcaa aactatatta aaaaccgatg cattgtttta
     2761 gggaaattaa tgagcctttt gttcaacact aaaaacaata aaattaaaat tttggcttca
     2821 tcatttgacc ttttttaagt tcgaaaactt ttctcgtatt tctgaaccgc caattttttc
     2881 acacatctct agacttttgg tgcccgttcc agaaagttaa gtaattgcta ttctaagaaa
     2941 gttctcaaca ttgtttttta gttctgattg aattctgatg ttccaggaat atattttaaa
     3001 tttaatattc tttgcactat ttctatacta actaaataat aaatagtcta agtatgttca
     3061 atgagaacaa aaaagctcta tctatatttt tctatcctat ttcatttata tccttttcat
     3121 tttgaactca ccagcttctc ttcttcttct tgtccatata cttcttaaaa gtctcgaact
     3181 tctctctctc actctccatc aatttcttta tggctaccgc ttgcgctctg ccgcttccga
     3241 agaaagaagg cgttggcaga gctctcaatt ctattttttc tcgccgtagg ctatcgattt
     3301 ctcagcttct cttctctctt cctcactcca ccaccaccac ttcgggcttc ttcttttctt
     3361 cattttcgtc ttcttcttca tcctcttctt tttttttcag ttccccctca ctcctcccct
     3421 tatatgcgcg tgcgagaggg gtgcaaaagc agcgcgcatc cgagaattga aacgagaaaa
     3481 cggagacgca gcagcagttc gtcctcagaa aaatagccag taaaaagaga aaaagataga
     3541 gagagaccta tcgcatttta ttttcaaatt gcaattcctg caattcatgt gtgcgtgtgc
     3601 ccattttcaa ttcttcccgt tatttttcag accattacca acgttctttc tagatcttga
     3661 gacaatcttc cttctgctca atcgttcgtc gtgaagacgg catcgacgac gacgacgatc
     3721 agcagtaaag gatcccccgg gctgcaggaa tgtcaaatat cagttggtac cgtcattgtt
     3781 cggttcggct tcaactcgtc acacttgcac ttttcctttt attaggctcc gcctctttag
     3841 gctccgccca cattgatgaa gagttcgaag atgacgtcac gacgacaata tcatcgattg
     3901 cgtcaccgat gaggcggacg tacacaaatg agtgggcggt gcggatagcg ggtgggaagg
     3961 ttgaagaagc gaaccgactg gcgaacaaat acgggtacac gaatttgggt ccgataattc
     4021 cgggtgacga gtactattta ttccgtgacg atcgaaaaaa gagtcgatcg tcgcgaaaaa
     4081 cgcgaagctt atcagcaaat cagcttcaac acgaggaaga tgtcatgtgg atggaacagc
     4141 aagtggcgaa gcggagagtg aaaagaggat atcgaaggat tcgacgacat actgatgata
     4201 atgatatttt tgaagaggat gatgatggta ctcaaattag caagtcgaga aatagaaaac
     4261 atccggatcc gaacgatccg ttgtggactg atatgtggta tttgaatcga ggtgaacatc
     4321 attctgactc aacaacccgt atggatcaca atgtaaaaga agcctgggac ctcgggtaca
     4381 caggaaaagg agtcgtcgtc acgattcttg acgacggttt ggaaagaact cacccagata
     4441 tttctccgaa ttacgatgaa cgtgcttcat atgacgtcaa cgatcgagat aatgacccga
     4501 tgccaagata cgaattttcg gatgaaaatc gacatggaac tcggtgtgct ggagaagttg
     4561 cggcgatttt taataatagt ttatgtattg ttggaatagc ttacaatgca aatatcggtg
     4621 gaatcagaat gctcgatggt gacgtcacag atgccgtcga agccgcttcc gttgggcata
     4681 atgcagatta cattgatatt tatagcgcat cctggggacc ggatgacgat ggccgaaccg
     4741 tcgacggccc cgcaaaactc acaagaagtg ccttcgaaaa aggaatcacc atgggtcgaa
     4801 aaggaaaagg ctcaattttt gtatgggcat caggaaatgg aggaaaagac gcggattcgt
     4861 gtaattgtga tgggtacacc aactccatat acacgttatc catatcaagt gccactgaaa
     4921 atggaaatat cccatggtac tctgaagctt gcagtagtac ccttgcgact acctactcta
     4981 gtggagcaac tggcgaaaag atgatcctaa ccacagatct acaccacgcg tgcacaaata
     5041 tgcacacggg gaccagtgca agtgctccgt tggctgctgg aattgttgct cttgctctag
     5101 aagccaaccc gaatctcacg tggcgagacc ttcaacatat tgtgatacga actgcaaagc
     5161 ctatcaattt aagagcagga gattggacta caaatggggt tgggagaaat gtgtcccata
     5221 gttttggtta tgggttgatg gatgctggag ccatggtgaa gctggcaaag atctggaaga
     5281 aggttgatga gcagcatcgg tgcaggcagt tttatccgtc gagatataag tgagtagtta
     5341 tcacccaaat ttatgaaaaa ttccagaagt ttctcgaatt ttgcaaaagt tttcaaaagt
     5401 ttctaaacat ttctaaaact ttcctgctgt tttcagaatt tttaagaatt ttccgttttc
     5461 aggagctatt aaaatttttt tagactttct agaagtttgt caaacattta aaaatgtttc
     5521 ctaatactcg agaaaatttt aaaaatttac tgaaaatccc tgagatcttc aaaaattttt
     5581 gacaagtttc tagacgtttc tcaaatcttc cagaagttta tagaagtcta cagaaatttc
     5641 caaaaatttc tcgatttttc cagaagtttc tagaagtttc agaagttcac acaattttcc
     5701 agaaatttcc agggacttct acgcgttttc agaagtttcc gaaaattcta gaaattgaga
     5761 aaatgtttag aaattccaaa aaactccaag tttttagaaa tatccccaac ggaaaccgcc
     5821 ttcaactcca actttactcc gacggatgtt atggtggagc cgatgagaat aaggtgtctt
     5881 atgtggagca cgtgcaggca atcgtcacat tgaaagctcc aaaacgtgga gatttgcaaa
     5941 tctacttgac ttctccgtca ggtaatgatt ttgaaaataa atttaggatg taaaactgac
     6001 tttttttaaa ccttcataac tttttttgac gaaaagctag aacattggct caaaaagcga
     6061 aaatgctcag ctgcaaattt caaatttaaa aaaatttaaa acgttcaggc acaaaatcca
     6121 cgttgctcac aaaaagagca cgcgacacat cacgaagtgg cttcacagat tgggctttca
     6181 tgacgaccca taactggggg gagcaggccg ccgggctgtg gattctggaa atcgataatg
     6241 atggatggga tggtgagtat tgatttctga aaattcattg atttttagga gtgattatcg
     6301 atttttgcag acgccgagct tgtaaaatgg gaactcgtgc tctacggaac tgatcgggaa
     6361 accggcgact ttggaggcca acatgcttca ccgttagccg tcagaagtgt ccagatggaa
     6421 gccacgtcat ccggcaccca atattcaatt ttccacgtca tcactcttgt catattaaca
     6481 ttttcccaga ttttatatta aggtaccatg gtattgatat ctgagctccg catcggccgc
     6541 tgtcatcaga tcgccatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa
     6601 catccctaca tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa
     6661 acttcttctt aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg
     6721 tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc
     6781 ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt
     6841 tttttttact tctgataaat tttttttgaa acatcataga aaaaaccgca cacaaaatac
     6901 cttatcatat gttacgtttc agtttatgac cgcaattttt atttcttcgc acgtctgggc
     6961 ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt ggagtatttt tggaattttt
     7021 caatcaagtg aaagtttatg aaattaattt tcctgctttt gctttttggg ggtttcccct
     7081 attgtttgtc aagagtttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga
     7141 gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag
     7201 aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata
     7261 cattcccaat ataccaaaca taactgtttc ctactagtgg ccacgtgggc cgtgcacctt
     7321 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     7381 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     7441 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     7501 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     7561 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7621 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7681 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7741 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     7801 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7861 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     7921 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7981 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     8041 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     8101 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     8161 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     8221 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     8281 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8341 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     8401 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     8461 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     8521 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8581 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8641 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8701 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8761 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8821 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8881 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8941 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9001 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9061 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9121 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     9181 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9241 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9301 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     9361 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9421 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     9481 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     9541 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     9601 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9661 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9721 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9781 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9841 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9901 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9961 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    10021 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    10081 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    10141 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    10201 ga
//
