LOCUS       sequence-347054-        9589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3148
FEATURES             Location/Qualifiers
     source          1..9589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2343..2367)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2375..2416
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2527..3115
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2580..2600)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2998..3009)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3187..3420
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3498..3619
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3535..3554)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3589..3608
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3659..3794
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3814..3833)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3815..3833)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3843..3860)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3843..3859)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3852..3874)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4001..4456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4088..4107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4298..4319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4482..4586
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4587..4655,4656..5447)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4805..4824)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5618..6206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6107..6126
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6360..6377
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6494..6515
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6530..6535,6536..6553,6554..6560)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6568..6584
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6573..6595
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6592..6608
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6592..6608
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6627..6647
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6629..6647
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6675..6901
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6902..7082
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7129..7254
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7747..7980
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8165..8209
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8440..8650
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8462..8658
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8509..8644
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8690..9292
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8690..8709)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9186..9206
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9335..9452
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgtcccc gtgtcctccc cagcagagca ggaacagggt
     1261 gatacagctg tccacttcag agctaggaga gatggaactg acttggcagg agatcatgtc
     1321 catcaccgag ctgcagggtc tgaatgctcc aagtgagcca tcatttgagc cccaagcccc
     1381 agctccatac cttggacctc caccacccac aacttactgc ccctgctcaa tccacccaga
     1441 ttctggcttc ccacttcctc caccacctta tgagctccca gcatccacat cccatgtccc
     1501 agatccccca tactcctatg gcaacatggc cataccagtc tccaagccac tgagcctctc
     1561 aggcctgctc agtgagccgc tccaagaccc cttagccctc ctggacattg ggctgccagc
     1621 agggccacct aagccccaag aagacccaga atccgactca ggattatccc tcaactatag
     1681 cgatgctgaa tctcttgagc tggaggggac agaggctggt cggcggcgca gcgaatatgt
     1741 agagatgtac ccagtggagt acccctactc actcatgccc aactccttgg cccactccaa
     1801 ctataccttg ccagctgctg agaccccctt ggccttagag ccctcctcag gccctgtgcg
     1861 ggctaagccc actgcacggg gggaggcagg gagtcgggat gaacgtcggg ccttggccat
     1921 gaagattcct tttcctacgg acaagattgt caacttgccg gtagatgact ttaatgagct
     1981 attggcaagg tacccgctga cagagagcca gctagcgcta gtccgggaca tccgacgacg
     2041 gggcaaaaac aaggtggcag cccagaactg ccgcaagagg aagctggaaa ccattgtgca
     2101 gctggagcgg gagctggagc ggctgaccaa tgaacgggag cggcttctca gggcccgcgg
     2161 ggaggcagac cggaccctgg aggtcatgcg ccaacagctg acagagctgt accgtgacat
     2221 tttccagcac cttcgggatg aatcaggcaa cagctactct cctgaagagt acgcgctgca
     2281 acaggctgcc gatgggacca tcttccttgt gccccggggg accaagatgg aggccacaga
     2341 ctgcccaact ttcttgtaca aagtggttga tatcggtaag cctatcccta accctctcct
     2401 cggtctcgat tctacgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     2461 gctttatata tcttgtggaa aggacgacat acccctggga cacatcattg aacgcgttaa
     2521 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2581 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2641 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2701 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2761 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2821 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2881 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     2941 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3001 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     3061 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     3121 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3181 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     3241 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3301 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3361 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3421 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     3481 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3541 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3601 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     3661 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3721 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3781 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     3841 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3901 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3961 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     4021 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4081 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4141 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4201 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4261 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4321 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4381 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4441 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4501 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4561 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4621 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4681 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4741 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4801 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4861 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4921 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4981 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5041 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5101 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5161 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5221 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5281 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5341 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5401 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5461 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5521 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5581 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5641 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5701 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5761 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5821 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5881 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5941 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6001 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6061 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6121 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6181 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6241 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6301 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6361 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6421 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     6481 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     6541 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     6601 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     6661 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     6721 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     6781 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     6841 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     6901 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6961 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7021 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7081 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     7141 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     7201 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     7261 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     7321 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     7381 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     7441 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     7501 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     7561 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     7621 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     7681 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     7741 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     7801 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     7861 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     7921 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     7981 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     8041 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     8101 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     8161 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     8221 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     8281 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     8341 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     8401 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8461 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8521 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8581 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8641 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
     8701 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     8761 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     8821 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     8881 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
     8941 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     9001 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     9061 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     9121 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     9181 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     9241 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
     9301 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     9361 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     9421 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     9481 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
     9541 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
