LOCUS       sequence-347071-       10450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10450)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3150
FEATURES             Location/Qualifiers
     source          1..10450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3204..3228)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3236..3277
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3388..3976
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3441..3461)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3859..3870)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4048..4281
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4359..4480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4396..4415)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4450..4469
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4520..4655
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4675..4694)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4676..4694)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4704..4721)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4704..4720)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4713..4735)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4862..5317
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4949..4968)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5159..5180
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5343..5447
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5448..5516,5517..6308)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5666..5685)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6479..7067
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6968..6987
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7221..7238
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7355..7376
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7391..7396,7397..7414,7415..7421)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7429..7445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7434..7456
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7453..7469
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7453..7469
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7488..7508
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7490..7508
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7536..7762
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7763..7943
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7990..8115
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8608..8841
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9026..9070
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9301..9511
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9323..9519
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9370..9505
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9551..10153
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9551..9570)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10047..10067
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10196..10313
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgcc aggaccccct agaagcctag aaatgggact
     1261 gttgacattt agggatgtgg ccatagaatt ctctctggag gagtggcaac acctggacat
     1321 tgcacagcag aatttatata gaaatgtgat gttagagaac tacagaaacc tggccttcct
     1381 gggtattgct gtctctaagc cagacctgat cacctgtctg gaacaaggga aagagccctg
     1441 gaatatgaag cgacatgaga tggtggatga acccccaggt atgtgtcctc attttgctca
     1501 agacctttgg ccagagcagg gcatggaaga ttcttttcaa aaagcaatac tgagaagata
     1561 tggaaaatat ggacatgaga atttacagtt aagaaaaggc tgtaaaagtg tggatgagta
     1621 taaggtgaac aaagaaggtt ataatggact taaccagtgt ttcacaactg cccagagcaa
     1681 agtatttcaa tgtgataaat atttgaaagt cttctataaa tttttaaatt caaacagacc
     1741 taagataaga catactgaaa agaaatcttt caaatgtaaa aaacgtgtca aattattttg
     1801 catgctttca cataaaaccc aacacaaaag catttatcat agagagaagt cctacaaatg
     1861 taaagaatgt ggaaaaacct ttaattggtc ctcaaccctt actaatcata ggaaaattta
     1921 tactgaagag aaaccttaca aatgtgaaga atataacaaa tctcctaagc aactctcaac
     1981 ccttactaca catgaaataa ttcatgctgg agagaaactc tacaaatgtg aagaatgtgg
     2041 tgaagctttt aatcgatcct caaatcttac tacacataag ataattcata ctggagagaa
     2101 accttacaag tgtgaagaat gtggcaaagc atttatctgg tcctcaaccc ttactgagca
     2161 taagaaaatt catactagaa agaaacccta caagtgtgaa gaatgtggca aagcatttat
     2221 atggtcctca acactaacta gacataagag gatgcacact ggagagaaac cctacaaatg
     2281 tgaagaatgt ggcaaagctt ttagccagtc ctcaaccctt actacacata agataattca
     2341 tactggagag aaacgctaca aatgcttaga atgtggcaaa gcttttaagc aactctcaac
     2401 tcttactaca cataaaataa ttcatgttgg agagaaactc tacaaatgtg aagaatgcgg
     2461 caaaggtttt aatcgatctt caaatcttac tacacataag ataattcata ctggagagaa
     2521 accttacaag tgtgaagaat gtggcaaagc atttatctgg tcctcaaccc ttactaaaca
     2581 taagagaatt catactagag agaaacccta caaatgtgaa gaatgtggca aggcatttat
     2641 atggtcctca accctaacta gacataagag gatgcacact ggagagaaac cctacaaatg
     2701 tgaagaatgt ggcaaatctt ttagccaatc ctcaaccctt actacacata agataattca
     2761 tactggagag aaaccctaca aatgtgaaga atgtggcaaa gcctttaact ggtcctcaac
     2821 tcttactaaa cataagataa ttcatactga agagaaaccc tacaaatgtg aaaaatgtgg
     2881 caaagccttt aagcagtctt caatccttac taaccataag agaattcata ctggagagaa
     2941 accctataaa tgtgaagaat gtggcaaatc ttttaaccgg tcttcaactt ttactaaaca
     3001 taaggtaatt catactggag taaaacccta caaatgtgaa gaatgtggca aagcattttt
     3061 ctggtcctca accctaacta aacataagag aattcatact ggagagcaac cctacaaatg
     3121 ggaaaaattt ggcaaagcct ttaatcggtc ctcgcacctc accacagata agataactca
     3181 ttggagagaa atcttacaag tattgccaac tttcttgtac aaagtggttg atatcggtaa
     3241 gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgaacta gtccgtaact
     3301 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgatc cgtgttggtc
     3361 aagcgacgcg ccacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     3421 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3481 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3541 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3601 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3661 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3721 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3781 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3841 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3901 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3961 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     4021 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     4081 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4141 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4201 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4261 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     4321 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     4381 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4441 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     4501 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4561 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4621 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     4681 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4741 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4801 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4861 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4921 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4981 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5041 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5101 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5161 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5221 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5281 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     5341 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5401 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5461 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5521 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5581 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5641 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5701 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5761 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5821 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5881 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5941 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6001 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6061 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6121 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6181 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6241 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6301 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6361 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6421 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6481 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6541 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6601 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6661 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6721 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6781 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6841 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6901 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6961 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7021 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7081 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7141 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7201 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7261 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7321 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7381 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7441 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     7501 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     7561 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     7621 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     7681 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     7741 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     7801 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7861 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7921 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     7981 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     8041 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     8101 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     8161 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     8221 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     8281 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     8341 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     8401 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     8461 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     8521 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     8581 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     8641 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     8701 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     8761 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8821 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8881 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     8941 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     9001 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     9061 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     9121 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     9181 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     9241 acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     9301 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta
     9361 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9421 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9481 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc
     9541 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     9601 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     9661 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     9721 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
     9781 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9841 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9901 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     9961 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
    10021 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
    10081 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
    10141 cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
    10201 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
    10261 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
    10321 attacaggga cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg
    10381 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
    10441 agtgggaatt
//
