LOCUS       sequence-347072-       12227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12227)
  AUTHORS   McCobb DP, Fowler NL, Featherstone T, Lingle CJ, Saito M, Krause JE,
            Salkoff L
  TITLE     A human calcium-activated potassium channel gene expressed in 
            vascular smooth muscle.
  JOURNAL   Am J Physiol. 1995 Sep . 269(3 Pt 2):H767-77.
  PUBMED    7573516
REFERENCE   2  (bases 1 to 12227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Physiol. 1995 Sep . 269(3 Pt 2):H767-77."
COMMENT     Sequence Label: pM2AH + Slo1
FEATURES             Location/Qualifiers
     source          1..12227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2113..2705
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     5'UTR           2962..3004
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     primer_bind     complement(6829..6845)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6829..6845)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    6870..7004
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6894..6913)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6948..6967
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7429..7449)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7579..7644
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(8064..8858)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8175..8194)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8785..8804
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(9219..9548)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9233..9368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9533..9553
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(9591..9613)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9699..9839
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9754..9773
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9854..9871)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10025..10613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10105..10124)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10784..11575,11576..11644))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11407..11426
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11645..11749)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11817..11835
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gctgggtgag gccatgtgta tacatatctg cagactgtgt atgtggagct taagacctat
       61 ctcagtgcac cagtacctga tactgggaga tgtgtgactt agtcctgatc tgcctggaac
      121 acatcactcc tgccagccgg gcactaacaa ttatcaccca atccaggact taaactgtga
      181 tagagtggct gatgttctgc ctttaaatag agtgtcccaa aagatgggac cactggtcag
      241 ctgccatgga ctagattctc caccggttgg ggacaatctg gtcactttgc tgcccaatcc
      301 ggacctggag ccaccactgc acaagtgtca agtactggca gaagcccatg ggtggaagaa
      361 agacctctgc gactggctga ttgacccctg ctgaaagccg aggaccttgt ccacagacgg
      421 gaacagttct cttcatgaat aaaggtcaga gacaagtggg tgctgccatg gtagacaaca
      481 caatgtcatc tgggatggct caacccccca gtacatcagc gctgcagatg cctttgccat
      541 ctcttggatg ccctgatgaa gccaacaact gaatagataa aagctttcct caccaaacga
      601 gagacggcct ctacaatcat caagaagata ctggaagaaa tcttcctctt ggaatgccca
      661 aggtaatctg gtcagacaac ggccctactt tcgttgccaa ggtaagccag ggtgtggcca
      721 agtatttaga ggtcgattga aaattacatt gtatttacag acctcaaagt tcaggacagg
      781 tagggtaaat aaataaaact ctaaaagaga ccctgaccaa attaaccatg gagactggca
      841 cagactgggt gacactcctt ccctcttgct ctcttcagag caagaaatac cccttccaga
      901 ttcagcctta ccccctttga gatcttatat ggggcctcag ccccactgac tgtattagat
      961 gatgttactg aaccaacatg tcatagtgcc atagtaataa tgatttgtgt gccaggctaa
     1021 aagacctaca ggtgatacag aaagaaatct gctcagagct ggcagcagcc tatgccctgg
     1081 ggacccctga gacatctcac cagttccagg tcggagactc ggctacatac gatggcaccg
     1141 aacccagata ctcactggaa aggaccgtac ctggttctgc tgaccaccct gacagccatc
     1201 agctctcaac cctcaccagc cgtgtactag ctgttggggc tgagagctgg gacctagagg
     1261 gaaactcaga tcttcaaaaa gctccctaga cttaatttca tgtttgcccc gggttttatc
     1321 aagataggtg tggggatagg cttgatttct attacaaatg atgtaacatt gcatatgtta
     1381 gtactcctaa cacttcttgg gactgtgcct cagggatcac aatctatata agtttagaag
     1441 ttctaaaagc tagtcatgac cttggtgtgt aggtttagat agtgtccaga ttagaatcct
     1501 gatgctgaga gttgaaagtt acttaaggct atctaggtgc tgcaagggca gcacaaggac
     1561 atctgccatt gccctgcaag gctcggttta tagaaaattc agaactgcca ttgactcaga
     1621 tataaaaaga ataaaagact ttttagctga cctccaagaa tacctaacct ccctctcaga
     1681 ggtagtcctt cagaatagaa aaagattaga cctgatattc cttaaagaaa gaacctgtgt
     1741 gctacactaa aagaatgttg cttctatgcg gaccacacag gactagtgag agacagcatg
     1801 gccaaattga gagagaggct gaatcagaga cagaaactgt ttgagtcaac tcaaagatgg
     1861 tttgagggac tgtttaacag atccccttgg tttaccacct tgatatctac cattatggga
     1921 cccctcattg tactcctaat gattttgctc ttcggaccct gcattcttaa tcgattagtc
     1981 caatttgtta aagacaggat atcagtggtc caggctctag ttttgactca acaatatcac
     2041 cagctgaagc ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag
     2101 aaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     2161 attttacaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg
     2221 aacagagaaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     2281 ggcccagggc caagaacaga tagaacagct gaatatgggc caaacaggat atctgtggta
     2341 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct
     2401 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
     2461 tgtgccttat ttaaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
     2521 ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc tccgattgac
     2581 tgagtcgccc gggtacccgt atatccaata aaccctcttg cagttgcatc cgacttgtgg
     2641 tctcgctgtt ccttgggagg gtctcctcta agtgattgac tacccgttag tgggggtctt
     2701 tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg acccaccacc
     2761 gggaggcaag ccggccggcg tttgtcttgt ctgttgtgtc ttgtcctgtg aacgatcgat
     2821 caataggctc agatctgggg actatctggg cgggccagag aaggagctga cgagctcgga
     2881 cttctccccc gcagccctgg aagacgttcc aagggtggtt ggaggagagg gagatccgga
     2941 tcttaagggc ccctcgagct tcttgttctt tttgcagaag ctcagaataa acgctcaact
     3001 ttggcagaat cgataagctt gatcgagccc ctgcgcctgc cgcccattgc tagctatggc
     3061 aaacggtggc ggcggcggcg gcggcagcag cggcggcggc ggcggcggcg gcggaggcag
     3121 cggtcttaga atgagcagca atatccacgc gaaccatctc agcctagacg cgtcctcctc
     3181 ctcctcctcc tcctcttcct cttcttcttc ttcctcctcc tcttcctcct cgtcctcggt
     3241 ccacgagccc aagatggatg cgctcatcat cccggtgacc atggaggtgc cgtgcgacag
     3301 ccggggccaa cgcatgtggt gggctttcct ggcctcctcc atggtgactt tcttcggggg
     3361 cctcttcatc atcttgctct ggcggacgct caagtacctg tggaccgtgt gctgccactg
     3421 cgggggcaag acgaaggagg cccagaagat taacaatggc tcaagccagg cggatggcac
     3481 tctcaaacca gtggatgaaa aagaggaggc agtggccgcc gaggtcggct ggatgacctc
     3541 cgtgaaggac tgggcggggg tgatgatatc cgcccagaca ctgactggca gagtcctggt
     3601 tgtcttagtc tttgctctca gcatcggtgc acttgtaata tacttcatag attcatcaaa
     3661 cccaatagaa tcctgccaga atttctacaa agatttcaca ttacagatcg acatggcttt
     3721 caacgtgttc ttccttctct acttcggctt gcggtttatt gcagccaacg ataaattgtg
     3781 gttctggctg gaagtgaact ctgtagtgga tttcttcacg gtgccccccg tgtttgtgtc
     3841 tgtgtactta aacagaagtt ggcttggttt gagattttta agagctctga gactgataca
     3901 gttttcagaa attttgcagt ttctgaatat tcttaaaaca agtaattcca tcaagctggt
     3961 gaatctgctc tccatattta tcagcacgtg gctgactgca gctgggttca tccatttggt
     4021 ggagaattca ggggacccat gggaaaattt ccaaaacaac caggctctca cctactggga
     4081 atgtgtctat ttactcatgg tcacaatgtc caccgttggt tatggggatg tttatgcaaa
     4141 aaccacactt gggcgcctct tcatggtctt cttcatcctc gggggactgg ccatgtttgc
     4201 cagctacgtc cctgaaatca tagagttaat aggaaaccgc aagaaatacg ggggctccta
     4261 tagtgcggtt agtggaagaa agcacattgt ggtctgcgga cacatcactc tggagagtgt
     4321 ttccaacttc ctgaaggact ttctgcacaa ggaccgggat gacgtcaatg tggagatcgt
     4381 ttttcttcac aacatctccc ccaacctgga gcttgaagct ctgttcaaac gacattttac
     4441 tcaggtggaa ttttatcagg gttccgtcct caatccacat gatcttgcaa gagtcaagat
     4501 agagtcagca gatgcatgcc tgatccttgc caacaagtac tgcgctgacc cggatgcgga
     4561 ggatgcctcg aatatcatga gagtaatctc cataaagaac taccatccga agataagaat
     4621 catcactcaa atgctgcagt atcacaacaa ggcccatctg ctaaacatcc cgagctggaa
     4681 ttggaaagaa ggtgatgacg caatctgcct cgcagagttg aagttgggct tcatagccca
     4741 gagctgcctg gctcaaggcc tctccaccat gcttgccaac ctcttctcca tgaggtcatt
     4801 cataaagatt gaggaagaca catggcagaa atactacttg gaaggagtct caaatgaaat
     4861 gtacacagaa tatctctcca gtgccttcgt gggtctgtcc ttccctactg tttgtgagct
     4921 gtgttttgtg aagctcaagc tcctaatgat agccattgag tacaagtctg ccaaccgaga
     4981 gagccgtata ttaattaatc ctggaaacca tcttaagatc caagaaggta ctttaggatt
     5041 tttcatcgca agtgatgcca aagaagttaa aagggcattt ttttactgca aggcctgtca
     5101 tgatgacatc acagatccca aaagaataaa aaaatgtggc tgcaaacggc ttgaagatga
     5161 gcagccgtca acactatcac caaaaaaaaa gcaacggaat ggaggcatgc ggaactcacc
     5221 caacacctcg cctaagctga tgaggcatga ccccttgtta attcctggca atgatcagat
     5281 tgacaacatg gactccaatg tgaagaagta cgactctact gggatgtttc actggtgtgc
     5341 acccaaggag atagagaaag tcatcctgac tcgaagtgaa gctgccatga ccgtcctgag
     5401 tggccatgtc gtggtctgca tctttggcga cgtcagctca gccctgatcg gcctccggaa
     5461 cctggtgatg ccgctccgtg ccagcaactt tcattaccat gagctcaagc acattgtgtt
     5521 tgtgggctct attgagtacc tcaagcggga atgggagacg cttcataact tccccaaagt
     5581 gtccatattg cctggtacgc cattaagtcg ggctgattta agggctgtca acatcaacct
     5641 ctgtgacatg tgcgttatcc tgtcagccaa tcagaataat attgatgata cttcgctgca
     5701 ggacaaggaa tgcatcttgg cgtcactcaa catcaaatct atgcagtttg atgacagcat
     5761 cggagtcttg caggctaatt cccaagggtt cacacctcca ggaatggata gatcctctcc
     5821 agataacagc ccagtgcacg ggatgttacg tcaaccatcc atcacaactg gggtcaacat
     5881 ccccatcatc actgaactag tgaacgatac taatgttcag tttttggacc aagacgatga
     5941 tgatgaccct gatacagaac tgtacctcac gcagcccttt gcctgtggga cagcatttgc
     6001 cgtcagtgtc ctggactcac tcatgagcgc gacgtacttc aatgacaata tcctcaccct
     6061 gatacggacc ctggtgaccg gaggagccac gccggagctg gaggctctga ttgctgagga
     6121 aaacgccctt agaggtggct acagcacccc gcagacactg gccaataggg accgctgccg
     6181 cgtggcccag ttagctctgc tcgatgggcc atttgcggac ttaggggatg gtggttgtta
     6241 tggtgatctg ttctgcaaag ctctgaaaac atataatatg ctttgttttg gaatttaccg
     6301 gctgagagat gctcacctca gcacccccag tcagtgcaca aagaggtatg tcatcaccaa
     6361 cccgccctat gagtttgagc tcgtgccgac ggacctgatc ttctgcttaa tgcagtttga
     6421 ccacaatgcc ggccagtccc gggccagcct gtcccattcc tcccactcgt cgcagtcctc
     6481 cagcaagaag agctcctctg ttcactccat cccatccaca gcaaaccgac agaaccggcc
     6541 caagtccagg gagtcccggg acaaacagaa gtacgtgcag gaagagcggc tttgatatgt
     6601 gtatccaccg ccactgtgtg aaactgtatc tgccactcat ttccccagtt ggtgtttcca
     6661 acaaagtaac tttccctgtt ttcccctgta gtccccccct ttttttttac acatatttgc
     6721 atatgtatga tagtgtgcat gtggttgtca tttttatttc accaccataa aacccttgag
     6781 cacaacagca aataagcaga cgggctccgg aattcctgca gcccggggga tccactagtt
     6841 ctagagcggc cgcccgcgga agcttccttg atccagacat gataagatac attgatgagt
     6901 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     6961 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     7021 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     7081 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     7141 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     7201 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     7261 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     7321 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     7381 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     7441 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     7501 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     7561 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     7621 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     7681 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cggaccaaag cggccatcgt
     7741 gcctccccac tcctgcagtt cgggggcatg gatgcgcgga tagccgctgc tggtttcctg
     7801 gatgccgacg gatttgcact gccggtagaa ctccgcgagg tcgtccagcc tcaggcagca
     7861 gctgaaccaa ctcgcgaggg gatcgagccc ggggtgggcg aagaactcca gcatgagatc
     7921 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     7981 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
     8041 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     8101 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     8161 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     8221 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     8281 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     8341 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     8401 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     8461 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     8521 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     8581 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     8641 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac
     8701 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     8761 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     8821 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     8881 tcttgatcag atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
     8941 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg
     9001 cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc
     9061 tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc
     9121 cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc
     9181 ttgcgccctg agtgcttgcg gcagcgtgaa gctagctttt tgcaaaagcc taggcctcca
     9241 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     9301 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     9361 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     9421 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     9481 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     9541 tgacacacat tccacagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     9601 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     9661 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     9721 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     9781 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     9841 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     9901 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     9961 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    10021 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    10081 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    10141 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    10201 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    10261 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    10321 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    10381 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    10441 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
    10501 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    10561 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    10621 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    10681 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    10741 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    10801 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    10861 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    10921 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    10981 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    11041 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
    11101 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    11161 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    11221 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    11281 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    11341 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    11401 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    11461 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    11521 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    11581 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    11641 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    11701 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    11761 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
    11821 ggcgtatcac gaggcccttt cgtcttcaag aattcctggc taaagatcag tgtgcctaat
    11881 acaaagaaaa aggacattgg gtcagggact gccccagaaa gaacaagagg ggacactaga
    11941 gagcctcttg gtgctggcta tgcacagaga tagaagggaa gcccgcccag ttgttttgtg
    12001 gagacaggga cccaatgctc tgtgctccta tgccccccag tgggccaata tccaaagacc
    12061 ttgagtgcaa ggggctactg gcaacaaaca atatttatag actgcccgaa gaacagtgga
    12121 ccttggcgtg ggccgggtaa cccactagta catggtcatc cctgactgcc cctatccctt
    12181 gctcatgaga aatttgctgt ccagaatggc ttgtgtgggc taaaatg
//
