LOCUS       sequence-347073-       10347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrated in CHO-K1 to make parental polyclonal lines for all other
            MultiFate cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Zhu R, Del Rio-Salgado JM, Garcia-Ojalvo J, Elowitz MB
  TITLE     Synthetic multistability in mammalian cells.
  JOURNAL   Science. 2022 Jan 21;375(6578):eabg9765. doi: 
            10.1126/science.abg9765. Epub 2022 Jan 21.
  PUBMED    35050677
REFERENCE   2  (bases 1 to 10347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-01-21"; volume: "375(6578):eabg9765. doi"; pages: " 
            10.1126/science.abg9765. Epub 2022 Jan 21"
COMMENT     Sequence Label: MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
FEATURES             Location/Qualifiers
     source          1..10347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1474..2656
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1705..2647
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2604..2624
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     2658..2682
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2764..3507
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             3514..3570
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3574..4509
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4543..4983
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             4984..5328
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     complement(5354..5371)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5360..5567
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5600..5620)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5605..5934
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5785..5920
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5847..5866
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6001..6795
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6055..6074)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6665..6684
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6855..6875)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6917..7038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6954..6973)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7008..7027
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7801..7835
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8326..8342)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8326..8342)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8339..8361)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8350..8366
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8374..8380,8381..8398,8399..8404))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8419..8440
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8557..8574)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8728..9316)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8808..8827)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9487..10278,10279..10347))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10110..10129
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagga atcccaggga ccgtcgttaa
     1381 actcccacta acgtagaacc cagagatcgc tgcgttcccg ccccctcacc cgcccgctct
     1441 cgtcatcact gaggtggaga agagcatgcg tgaggctccg gtgcccgtca gtgggcagag
     1501 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     1561 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     1621 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     1681 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     1741 tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc tgcagtacgt
     1801 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     1861 aggagcccct tcgcctcgtg cttgagttga ggcctggctt gggcgctggg gccgccgcgt
     1921 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     1981 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     2041 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     2101 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     2161 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     2221 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     2281 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     2341 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     2401 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     2461 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     2521 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     2581 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     2641 atttcaggtg tcgtgagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     2701 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaattc
     2761 accatgtcta gactggacaa gagcaaagtc ataaactctg ctctggaatt actcaatgga
     2821 gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct
     2881 accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg
     2941 ctggacaggc atcataccca ctcctgcccc ctggaaggcg agtcatggca agactttctg
     3001 cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg
     3061 catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc
     3121 ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac
     3181 tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag
     3241 acacctacca ccgattctat gcccccactt ctgaaacaag caattgagct gttcgaccgg
     3301 cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa
     3361 cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg
     3421 ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac
     3481 gattttgacc ttgacatgct ccccgggggc agcgctacta acttcagcct gctgaagcag
     3541 gctggagacg tggaggagaa ccctggacct ggcgctggag acatgagagc tgccaacctt
     3601 tggccaagcc cgctcatgat caaacgctct aagaagaaca gcctggcctt gtccctgacg
     3661 gccgaccaga tggtcagtgc cttgttggat gctgagcccc ccatactcta ttccgagtat
     3721 gatcctacca gacccttcag tgaagcttcg atgatgggct tactgaccaa cctggcagac
     3781 agggagctgg ttcacatgat caactgggcg aagagggtgc caggctttgt ggatttgacc
     3841 ctccatgatc aggtccacct tctagaatgt gcctggctag agatcctgat gattggtctc
     3901 gtctggcgct ccatggagca cccagtgaag ctactgtttg ctcctaactt gctcttggac
     3961 aggaaccagg gaaaatgtgt agagggcatg gtggagatct tcgacatgct gctggctaca
     4021 tcatctcggt tccgcatgat gaatctgcag ggagaggagt ttgtgtgcct caaatctatt
     4081 attttgctta attctggagt gtacacattt ctgtccagca ccctgaagtc tctggaagag
     4141 aaggaccata tccaccgagt cctggacaag atcacagaca ctttgatcca cctgatggcc
     4201 aaggcaggcc tgaccctgca gcagcagcac cagcggctgg cccagctcct cctcatcctc
     4261 tcccacatca ggcacatgag taacaaaggc atggagcatc tgtacagcat gaagtgcaag
     4321 aacgtggtgc ccctctatga cctgctgctg gaggcggcgg acgcccaccg cctacatgcg
     4381 cccactagcc gtggaggggc atccgtggag gagacggacc aaagccactt ggccactgcg
     4441 ggctctactt catcgcattc cttgcaaaag tattacatca cgggggaggc agagggtttc
     4501 cctgccacag ctgtcgacgg tggttctggt ggtggttctg gtatgaagct actgtcttct
     4561 atcgaacaag catgcgatat ttgccgactt aaaaagctca agtgctccaa agaaaaaccg
     4621 aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct actctcccaa aaccaaaagg
     4681 tctccgctga ctagggcaca tctgacagaa gtggaatcaa ggctagaaag actggaacag
     4741 ctatttctac tgatttttcc tcgagaagac cttgacatga ttttgaaaat ggattcttta
     4801 caggatataa aagcattgtt aacaggatta tttgtacaag ataatgtgaa taaagatgcc
     4861 gtcacagata gattggcttc agtggagact gatatgcctc taacattgag acagcataga
     4921 ataagtgcga catcatcatc ggaagagagt agtaacaaag gtcaaagaca gttgactgta
     4981 tcggccaatt ttaatcaaag tgggaatatt gctgatagct cattgtcctt cactttcact
     5041 aacagtagca acggtccgaa cctcataaca actcaaacaa attctcaagc gctttcacaa
     5101 ccaattgcct cctctaacgt tcatgataac ttcatgaata atgaaatcac ggctagtaaa
     5161 attgatgatg gtaataattc aaaaccactg tcacctggtt ggacggacca aactgcgtat
     5221 aacgcgtttg gaatcactac agggatgttt aataccacta caatggatga tgtatataac
     5281 tatctattcg atgatgaaga taccccacca aacccaaaaa aagagtaagc tagcgaattc
     5341 gaatttaaat cggcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5401 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5461 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5521 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggacgc ggccgcaatt
     5581 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5641 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5701 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5761 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5821 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     5881 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     5941 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     6001 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     6061 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     6121 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     6181 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6241 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6301 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6361 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6421 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6481 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     6541 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     6601 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     6661 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     6721 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     6781 gacgagttct tctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     6841 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6901 tatcatgcgt taactaaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6961 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7021 actcatcaat gtatcttatc atgtctggaa ttgactcaaa tgatgtcaat tagtctatca
     7081 gaagctcatc tggtctccct tccgggggac aagacatccc tgtttaatat ttaaacagca
     7141 gtgttcccaa actgggttct tatatccctt gctctggtca accaggttgc agggtttcct
     7201 gtcctcacag gaacgaagtc cctaaagaaa cagtggcagc caggtttagc cccggaattg
     7261 actggattcc ttttttaggg cccattggta tggctttttc cccgtatccc cccaggtgtc
     7321 tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc
     7381 gtgcccgggc tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga
     7441 gcggagcccc gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct
     7501 gggggctttg ggggggggct gtccctgata tctataacaa gaaaatatat atataataag
     7561 ttatcacgta agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa
     7621 gtcacgtaaa agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca
     7681 gtgacactta ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt
     7741 cctaaatgca cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg
     7801 catgcgtcaa ttttacgcag actatctttc tagggttaat ctagctgcat caggatcata
     7861 tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg
     7921 aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag tttgccgagg
     7981 atgactgctg ctgcattgac gttgagcgaa aacgcacgtt taccatgatg attcgggaag
     8041 gtgtggccat gcacgccttt aacggtgaac tgttcgttca ggccacctgg gataccagtt
     8101 cgtcgcggct tttccggaca cagttccgga tggtcagccc gaagcgcatc agcaacccga
     8161 acaataccgg cgacagccgg aactgccgtg ccggtgtgca gattaatgac agcggtgcgg
     8221 cgctgggata ttacgtcagc gaggacgggt atcctggctg gatgccgcag aaatggacat
     8281 ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc
     8341 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8401 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8461 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8521 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8581 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8641 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8701 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8761 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8821 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8881 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8941 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9001 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9061 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9121 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     9181 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9241 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9301 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9361 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9421 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9481 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9541 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9601 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9661 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9721 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9781 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9841 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9901 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9961 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10021 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10081 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10141 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10201 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10261 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10321 ggcgacacgg aaatgttgaa tactcat
//
