LOCUS       sequence-347137-       13673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector for constitutive PLXNB2 overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13673)
  AUTHORS   Junqueira Alves C, Dariolli R, Haydak J, Kang S, Hannah T, Wiener 
            RJ, DeFronzo S, Tejero R, Gusella GL, Ramakrishnan A, Alves Dias R, 
            Wojcinski A, Kesari S, Shen L, Sobie EA, Rodrigues Furtado de 
            Mendonca JP, Azeloglu EU, Zou H, Friedel RH
  TITLE     Plexin-B2 orchestrates collective stem cell dynamics via actomyosin 
            contractility, cytoskeletal tension and adhesion.
  JOURNAL   Nat Commun. 2021 Oct 14;12(1):6019. doi: 10.1038/s41467-021-26296-7.
  PUBMED    34650052
REFERENCE   2  (bases 1 to 13673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-10-14"; volume: "12(1):6019. doi"; pages: " 
            10.1038/s41467-021-26296-7"
COMMENT     Sequence Label: pLV-hPLXNB2_PGK_Neo
FEATURES             Location/Qualifiers
     source          1..13673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4137
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4183..4693
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4612..4630
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    4748..4772
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10317..10341)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10372..10960
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10425..10445)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10843..10854)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        11003..11502
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11038..11061)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11435..11454
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11516..12319
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11579..11598)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12189..12208
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             12410..12643
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12715..12836
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12752..12771)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12806..12825
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12876..13011
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(13031..13050)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(13032..13050)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13060..13077)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13060..13076)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13069..13091)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13218..13646
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13305..13324)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13515..13536
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc
     4141 gatcacgaga ctagcctcga gaagcttgat atcgaattcc acggggttgg ggttgcgcct
     4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4501 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4681 cctctctccc cagggggatc caccggttcg tcgactagtc cagtgtggtg gaattctgca
     4741 gatatcaaca agtttgtaca aaaaagcaga agggccgtca aggcccacca tggcactgca
     4801 gctctgggcc ctgaccctgc tgggcctgct gggcgcaggt gccagcctga ggccccgcaa
     4861 gctggacttc ttccgcagcg agaaagagct gaaccacctg gctgtggatg aggcctcagg
     4921 cgtggtgtac ctgggggcgg tgaatgccct ctaccagctg gatgcgaagc tgcagctgga
     4981 gcagcaggtg gccacgggcc cggccctgga caacaagaag tgcacgccgc ccatcgaggc
     5041 cagccagtgc catgaggctg agatgactga caatgtcaac cagctgctgc tgctcgaccc
     5101 tcccaggaag cgcctggtgg agtgcggcag cctcttcaag ggcatctgcg ctctgcgcgc
     5161 cctgagcaac atctccctcc gcctgttcta cgaggacggc agcggggaga agtctttcgt
     5221 ggccagcaat gatgagggcg tggccacagt ggggctggtg agctccacgg gtcctggtgg
     5281 tgaccgcgtg ctgtttgtgg gcaaaggcaa tgggccacac gacaacggca tcatcgtgag
     5341 cactcggctg ttggaccgga ctgacagcag ggaggccttt gaagcctaca cggaccacgc
     5401 cacctacaag gccggctacc tgtccaccaa cacacagcag ttcgtggcgg ccttcgagga
     5461 cggcccctac gtcttctttg tcttcaacca gcaggacaag cacccggccc ggaaccgcac
     5521 gctgctggca cgcatgtgca gagaagaccc caactactac tcctacctgg agatggacct
     5581 gcagtgccgg gaccccgaca tccacgccgc tgcctttggc acctgcctgg ccgcctccgt
     5641 ggctgcgcct ggctctggca gggtgctata tgctgtcttc agcagagaca gccggagcag
     5701 tggggggccc ggtgcgggcc tctgcctgtt cccgctggac aaggtgcacg ccaagatgga
     5761 ggccaaccgc aacgcctgtt acacaggcac ccgggaggcc cgtgacatct tctacaagcc
     5821 cttccacggc gatatccagt gcggcggcca cgcgccgggc tccagcaaga gcttcccatg
     5881 tggctcggag cacctgccct acccgctggg cagccgcgac gggctcagag gcacagccgt
     5941 gctgcagcgt ggaggcctga acctcacggc cgtgacggtc gccgccgaga acaaccacac
     6001 tgttgctttt ctgggcacct ctgatggccg gatcctcaag gtgtacctca ccccagatgg
     6061 cacctcctca gagtacgact ctatccttgt ggagataaac aagagagtca agcgcgacct
     6121 ggtactgtct ggagacctgg gcagcctgta cgccatgacc caggacaagg tgttccggct
     6181 gccggtgcag gagtgcctga gctacccgac ctgcacccag tgccgcgact cccaggaccc
     6241 ctactgcggc tggtgcgtcg tcgagggacg atgcacccgg aaggccgagt gtccgcgggc
     6301 cgaggaggcc agccactggc tgtggagccg aagcaagtcc tgcgtggccg tcaccagcgc
     6361 ccagccacag aacatgagcc ggcgggccca gggggaggtg cagctgaccg tcagccccct
     6421 ccctgccctg agcgaggagg acgagttgct gtgccttttt ggggagtcgc cgccacaccc
     6481 cgcccgcgtg gagggcgagg ccgtcatctg caactcccca agcagcatcc ccgtcacacc
     6541 gccaggccag gaccacgtgg ccgtgaccat ccagctcctc cttagacgag gcaacatctt
     6601 cctcacgtcc taccagtacc ccttctacga ctgccgccag gccatgagcc tggaggagaa
     6661 cctgccgtgc atctcctgcg tgagcaaccg ctggacctgc cagtgggacc tgcgctacca
     6721 cgagtgccgg gaggcttcgc ccaaccctga ggacggcatc gtccgtgccc acatggagga
     6781 cagctgtccc cagttcctgg gacccagccc cctggtgatc cccatgaacc acgagacaga
     6841 tgtgaacttc cagggcaaga acctggacac cgtgaagggt tcctccctgc acgtgggcag
     6901 tgacttgctc aagttcatgg agccggtgac catgcaggaa tctgggacct tcgcctttcg
     6961 gaccccaaag ctgtcccacg atgccaacga gacgctgccc ctgcacctct acgtcaagtc
     7021 ttacggcaag aatatcgaca gcaagctcca tgtgaccctc tacaactgct cctttggccg
     7081 cagcgactgc agcctgtgcc gggccgctaa ccccgactac aggtgtgcgt ggtgcggggg
     7141 ccagagcagg tgcgtgtatg aggccctgtg caacaccacc tccgagtgcc cgccgcccgt
     7201 catcaccagg atccagcctg agacgggccc cctgggtggg ggcatccgca tcaccatcct
     7261 ggggtccaat ttgggcgtcc aagcagggga catccagagg atctctgtgg ccggccggaa
     7321 ctgctccttt cagccggaac gttactccgt gtccacccgg atcgtgtgtg tgatcgaggc
     7381 tgcggagacg cctttcacgg ggggtgtcga ggtggacgtc ttcgggaaac tgggccgttc
     7441 gcctcccaat gtccagttca ccttccaaca gcccaagcct ctcagtgtgg agccgcagca
     7501 gggaccgcag gcgggcggca ccacactgac catccacggc acccacctgg acacgggctc
     7561 ccaggaggac gtgcgggtga ccctcaacgg cgtcccgtgt aaagtgacga agtttggggc
     7621 gcagctccag tgtgtcactg gcccccaggc gacacggggc cagatgcttc tggaggtctc
     7681 ctacgggggg tcccccgtgc ccaaccccgg catcttcttc acctaccgcg aaaaccccgt
     7741 actgcgagcc ttcgagccgc tacgaagctt tgccagtggt ggccgcagca tcaacgtcac
     7801 gggtcagggc ttcagcctga tccagaggtt tgccatggtg gtcatcgcgg agcccctgca
     7861 gtcctggcag ccgccgcggg aggctgaatc cctgcagccc atgacggtgg tgggtacaga
     7921 ctacgtgttc cacaatgaca ccaaggtcgt cttcctgtcc ccggctgtgc ctgaggagcc
     7981 agaggcctac aacctcacgg tgctgatcga gatggacggg caccgtgccc tgctcagaac
     8041 agaggccggg gccttcgagt acgtgcctga ccccaccttt gagaacttca caggtggcgt
     8101 caagaagcag gtcaacaagc tcatccacgc ccggggcacc aatctgaaca aggcgatgac
     8161 gctgcaggag gccgaggcct tcgtgggtgc cgagcgctgc accatgaaga cgctgacgga
     8221 gaccgacctg tactgtgagc ccccggaggt gcagcccccg cccaagcggc ggcagaaacg
     8281 agacaccaca cacaacctgc ccgagttcat tgtgaagttc ggctctcgcg agtgggtgct
     8341 gggccgcgtg gagtacgaca cacgggtgag cgacgtgccg ctcagcctca tcttgccgct
     8401 ggtcatcgtg cccatggtgg tcgtcatcgc ggtgtctgtc tactgctact ggaggaagag
     8461 ccagcaggcc gaacgagagt atgagaagat caagtcccag ctggagggcc tggaggagag
     8521 cgtgcgggac cgctgcaaga aggaattcac agacctgatg atcgagatgg aggaccagac
     8581 caacgacgtg cacgaggccg gcatccccgt gctggactac aagacctaca ccgaccgcgt
     8641 cttcttcctg ccctccaagg acggcgacaa ggacgtgatg atcaccggca agctggacat
     8701 ccctgagccg cggcggccgg tggtggagca ggccctctac cagttctcca acctgctgaa
     8761 cagcaagtct ttcctcatca atttcatcca caccctggag aaccagcggg agttctcggc
     8821 ccgcgccaag gtctacttcg cgtccctgct gacggtggcg ctgcacggga aactggagta
     8881 ctacacggac atcatgcaca cgctcttcct ggagctcctg gagcagtacg tggtggccaa
     8941 gaaccccaag ctgatgctgc gcaggtctga gactgtggtg gagaggatgc tgtccaactg
     9001 gatgtccatc tgcctgtacc agtacctcaa ggacagtgcc ggggagcccc tgtacaagct
     9061 cttcaaggcc atcaaacatc aggtggaaaa gggcccggtg gatgcggtac agaagaaggc
     9121 caagtacact ctcaacgaca cggggctgct gggggatgat gtggagtacg cacccctgac
     9181 ggtgagcgtg atcgtgcagg acgagggagt ggacgccatc ccggtgaagg tcctcaactg
     9241 tgacaccatc tcccaggtca aggagaagat cattgaccag gtgtaccgtg ggcagccctg
     9301 ctcctgctgg cccaggccag acagcgtggt cctggagtgg cgtccgggct ccacagcgca
     9361 gatcctgtcg gacctggacc tgacgtcaca gcgggagggc cggtggaagc gcgtcaacac
     9421 ccttatgcac tacaatgtcc gggatggagc caccctcatc ctgtccaagg tgggggtctc
     9481 ccagcagccg gaggacagcc agcaggacct gcctggggag cgccatgccc tcctggagga
     9541 ggagaaccgg gtgtggcacc tggtgcggcc gaccgacgag gtggacgagg gcaagtccaa
     9601 gagaggcagc gtgaaagaga aggagcggac gaaggccatc accgagatct acctgacgcg
     9661 gctgctctca gtcaagggca cactgcagca gtttgtggac aacttcttcc agagcgtgct
     9721 ggcgcctggg cacgcggtgc cacctgcagt caagtacttc ttcgacttcc tggacgagca
     9781 ggcagagaag cacaacatcc aggatgaaga caccatccac atctggaaga cgaacagctt
     9841 accgctccgg ttctgggtga acatcctcaa gaacccccac ttcatctttg acgtgcatgt
     9901 ccacgaggtg gtggacgcct cgctgtcagt catcgcgcag accttcatgg atgcctgcac
     9961 gcgcacggag cataagctga gccgcgattc tcccagcaac aagctgctgt acgccaagga
    10021 gatctccacc tacaagaaga tggtggagga ttactacaag gggatccggc agatggtgca
    10081 ggtcagcgac caggacatga acacacacct ggcagagatt tcccgggcgc acacggactc
    10141 cttgaacacc ctcgtggcac tccaccagct ctaccaatac acgcagaagt actatgacga
    10201 gatcatcaat gccttggagg aggatcctgc cgcccagaag atgcagctgg ccttccgcct
    10261 gcagcagatt gccgctgcac tggagaacaa ggtcactgac ctctagggcc tcatgggccc
    10321 agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga caatcaacct
    10381 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
    10441 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
    10501 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
    10561 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
    10621 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
    10681 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
    10741 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
    10801 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
    10861 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
    10921 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattct gcagatatcc
    10981 ggactagtga tctaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
    11041 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
    11101 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
    11161 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
    11221 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
    11281 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
    11341 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
    11401 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
    11461 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag ccaatatggg
    11521 atcggccatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
    11581 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
    11641 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
    11701 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
    11761 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
    11821 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
    11881 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
    11941 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
    12001 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
    12061 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
    12121 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
    12181 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
    12241 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
    12301 tcttgacgag ttcttctgag gggatccgtc gagggcccgg tacctttaag accaatgact
    12361 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
    12421 gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
    12481 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    12541 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    12601 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
    12661 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
    12721 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    12781 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    12841 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
    12901 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
    12961 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
    13021 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
    13081 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
    13141 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
    13201 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
    13261 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
    13321 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
    13381 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
    13441 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
    13501 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
    13561 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
    13621 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa ttt
//
