LOCUS       sequence-347143-       11708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCasRx-METTL3 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11708)
  AUTHORS   Xia Z, Tang M, Ma J, Zhang H, Gimple RC, Prager BC, Tang H, Sun C, 
            Liu F, Lin P, Mei Y, Du R, Rich JN, Xie Q
  TITLE     Epitranscriptomic editing of the RNA N6-methyladenosine modification
            by dCasRx conjugated methyltransferase and demethylase.
  JOURNAL   Nucleic Acids Res. 2021 Jul 21;49(13):7361-7374. doi: 
            10.1093/nar/gkab517.
  PUBMED    34181729
REFERENCE   2  (bases 1 to 11708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2021-07-21- 21"; volume: "49"; issue: 
            "13"; pages: "7361-7374"
COMMENT     Sequence Label: pMSCV-dCasRx-METTL3-PURO
FEATURES             Location/Qualifiers
     source          1..11708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..54
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     46..63
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     47..63
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        78..304
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             305..485
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    532..657
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1150..1383
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1568..1612
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1879..1995
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             2028..2048
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2058..4955
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLAHWVVANNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFANKAVALEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             4965..5012
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6696..6719
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6735..6761
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6765..6818
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6825..7424
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6825..6844)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7321..7341
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7437..8025
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7490..7510)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7908..7919)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8574..8754
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8826..8947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8863..8882)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8917..8936
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8987..9122
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9155..9171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9155..9171)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9168..9190)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9179..9195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9203..9209,9210..9227,9228..9233))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9248..9269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9386..9403)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9557..10145)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(10316..11107,11108..11176))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10939..10958
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11177..11281)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11349..11367
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11405..11427)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11527..11546
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
       61 agtgccaagc tgacgcgtgt agtcttatgc aatactcttg tagtcttgca acatggtaac
      121 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
      181 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
      241 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
      301 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      361 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      421 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      481 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      541 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      601 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      661 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      721 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      781 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      841 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      901 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      961 gagcaaaaca aaagtaagac caccgcacag caagcggcca ctgatcttca gacctggagg
     1021 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1081 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1141 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1201 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1261 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1321 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1381 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1441 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1501 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1561 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1621 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     1681 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     1741 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     1801 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     1861 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     1921 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     1981 aaaattcaaa attttatcga tactagagct agagctagcg catgagcccc aagaagaaga
     2041 gaaaggtgga ggccagcatc gaaaaaaaaa agtccttcgc caagggcatg ggcgtgaagt
     2101 ccacactcgt gtccggctcc aaagtgtaca tgacaacctt cgccgaaggc agcgacgcca
     2161 ggctggaaaa gatcgtggag ggcgacagca tcaggagcgt gaatgagggc gaggccttca
     2221 gcgctgaaat ggccgataaa aacgccggct ataagatcgg caacgccaaa ttcagccatc
     2281 ctaagggcta cgccgtggtg gctaacaacc ctctgtatac aggacccgtc cagcaggata
     2341 tgctcggcct gaaggaaact ctggaaaaga ggtacttcgg cgagagcgct gatggcaatg
     2401 acaatatttg tatccaggtg atccataaca tcctggacat tgaaaaaatc ctcgccgaat
     2461 acattaccaa cgccgcctac gccgtcaaca atatctccgg cctggataag gacattattg
     2521 gattcggcaa gttctccaca gtgtatacct acgacgaatt caaagacccc gagcaccata
     2581 gggccgcttt caacaataac gataagctca tcaacgccat caaggcccag tatgacgagt
     2641 tcgacaactt cctcgataac cccagactcg gctatttcgg ccaggccttt ttcagcaagg
     2701 agggcagaaa ttacatcatc aattacggca acgaatgcta tgacattctg gccctcctga
     2761 gcggactggc gcactgggtg gtcgctaaca acgaagaaga gtccaggatc tccaggacct
     2821 ggctctacaa cctcgataag aacctcgaca acgaatacat ctccaccctc aactacctct
     2881 acgacaggat caccaatgag ctgaccaact ccttctccaa gaactccgcc gccaacgtga
     2941 actatattgc cgaaactctg ggaatcaacc ctgccgaatt cgccgaacaa tatttcagat
     3001 tcagcattat gaaagagcag aaaaacctcg gattcaatat caccaagctc agggaagtga
     3061 tgctggacag gaaggatatg tccgagatca ggaaaaatca taaggtgttc gactccatca
     3121 ggaccaaggt ctacaccatg atggactttg tgatttatag gtattacatc gaagaggatg
     3181 ccaaggtggc tgccgccaat aagtccctcc ccgataatga gaagtccctg agcgagaagg
     3241 atatctttgt gattaacctg aggggctcct tcaacgacga ccagaaggat gccctctact
     3301 acgatgaagc taatagaatt tggagaaagc tcgaaaatat catgcacaac atcaaggaat
     3361 ttaggggaaa caagacaaga gagtataaga agaaggacgc ccctagactg cccagaatcc
     3421 tgcccgctgg ccgtgatgtt tccgccttca gcaaactcat gtatgccctg accatgttcc
     3481 tggatggcaa ggagatcaac gacctcctga ccaccctgat taataaattc gataacatcc
     3541 agagcttcct gaaggtgatg cctctcatcg gagtcaacgc taagttcgtg gaggaatacg
     3601 cctttttcaa agactccgcc aagatcgccg atgagctgag gctgatcaag tccttcgcta
     3661 gaatgggaga acctattgcc gatgccagga gggccatgta tatcgacgcc atccgtattt
     3721 taggaaccaa cctgtcctat gatgagctca aggccctcgc cgacaccttt tccctggacg
     3781 agaacggaaa caagctcaag aaaggcaagc acggcatgag aaatttcatt attaataacg
     3841 tgatcagcaa taaaaggttc cactacctga tcagatacgg tgatcctgcc cacctccatg
     3901 agatcgccaa aaacgaggcc gtggtgaagt tcgtgctcgg caggatcgct gacatccaga
     3961 aaaaacaggg ccagaacggc aagaaccaga tcgacaggta ctacgaaact tgtatcggaa
     4021 aggataaggg caagagcgtg agcgaaaagg tggacgctct cacaaagatc atcaccggaa
     4081 tgaactacga ccaattcgac aagaaaagga gcgtcattga ggacaccggc agggaaaacg
     4141 ccgagaggga gaagtttaaa aagatcatca gcctgtacct caccgtgatc taccacatcc
     4201 tcaagaatat tgtcaatatc aacgccaggt acgtcatcgg attccattgc gtcgagcgtg
     4261 atgctcaact gtacaaggag aaaggctacg acatcaatct caagaaactg gaagagaagg
     4321 gattcagctc cgtcaccaag ctctgcgctg gcattgatga aactgccccc gataagagaa
     4381 aggacgtgga aaaggagatg gctgaaagag ccaaggagag cattgacagc ctcgagagcg
     4441 ccaaccccaa gctgtatgcc aattacatca aatacagcga cgagaagaaa gccgaggagt
     4501 tcaccaggca gattaacagg gagaaggcca aaaccgccct gaacgcctac ctgaggaaca
     4561 ccaagtggaa tgtgatcatc agggaggacc tcctgagaat tgacaacaag acatgtaccc
     4621 tgttcgcaaa caaggccgtc gccctggaag tggccaggta tgtccacgcc tatatcaacg
     4681 acattgccga ggtcaattcc tacttccaac tgtaccatta catcatgcag agaattatca
     4741 tgaatgagag gtacgagaaa agcagcggaa aggtgtccga gtacttcgac gctgtgaatg
     4801 acgagaagaa gtacaacgat aggctcctga aactgctgtg tgtgcctttc ggctactgta
     4861 tccccaggtt taagaacctg agcatcgagg ccctgttcga taggaacgag gccgccaagt
     4921 tcgacaagga gaaaaagaag gtgtccggca attccggatc cggaaagcga cctgccgcca
     4981 caaagaaggc tggacaggct aagaagaaga aaggtagttc catgtcggac acgtggagct
     5041 ctatccaggc ccacaagaag cagctggact ctctgcggga gaggctgcag cggaggcgga
     5101 agcaggactc ggggcacttg gatctacgga atccagaggc agcattgtct ccaaccttcc
     5161 gtagtgacag cccagtgcct actgcaccca cctctggtgg ccctaagccc agcacagctt
     5221 cagcagttcc tgaattagct acagatcctg agttagagaa gaagttgcta caccacctct
     5281 ctgatctggc cttaacattg cccactgatg ctgtgtccat ctgtcttgcc atctccacgc
     5341 cagatgctcc tgccactcaa gatggggtag aaagcctcct gcagaagttt gcagctcagg
     5401 agttgattga ggtaaagcga ggtctcctac aagatgatgc acatcctact cttgtaacct
     5461 atgctgacca ttccaagctc tctgccatga tgggtgctgt ggcagaaaag aagggccctg
     5521 gggaggtagc agggactgtc acagggcaga agcggcgtgc agaacaggac tcgactacag
     5581 tagctgcctt tgccagttcg ttagtctctg gtctgaactc ttcagcatcg gaaccagcaa
     5641 aggagccagc caagaaatca aggaaacatg ctgcctcaga tgttgatctg gagatagaga
     5701 gccttctgaa ccaacagtcc actaaggaac aacagagcaa gaaggtcagt caggagatcc
     5761 tagagctatt aaatactaca acagccaagg aacaatccat tgttgaaaaa tttcgctctc
     5821 gaggtcgggc ccaagtgcaa gaattctgtg actatggaac caaggaggag tgcatgaaag
     5881 ccagtgatgc tgatcgaccc tgtcgcaagc tgcacttcag acgaattatc aataaacaca
     5941 ctgatgagtc tttaggtgac tgctctttcc ttaatacatg tttccacatg gatacctgca
     6001 agtatgttca ctatgaaatt gatgcttgca tggattctga ggcccctggc agcaaagacc
     6061 acacgccaag ccaggagctt gctcttacac agagtgtcgg aggtgattcc agtgcagacc
     6121 gactcttccc acctcagtgg atctgttgtg atatccgcta cctggacgtc agtatcttgg
     6181 gcaagtttgc agttgtgatg gctgacccac cctgggatat tcacatggaa ctgccctatg
     6241 ggaccctgac agatgatgag atgcgcaggc tcaacatacc cgtactacag gatgatggct
     6301 ttctcttcct ctgggtcaca ggcagggcca tggagttggg gagagaatgt ctaaacctct
     6361 gggggtatga acgggtagat gaaattattt gggtgaagac aaatcaactg caacgcatca
     6421 ttcggacagg ccgtacaggt cactggttga accatgggaa ggaacactgc ttggttcgtt
     6481 ccaccagtca taaaccagat gaaatctatg gcatgattga aagactatct cctggcactc
     6541 gcaagattga gttatttgga cgaccacaca atgtgcaacc caactggatc acccttggaa
     6601 accaactgga tgggatccac ctactagacc cagatgtggt tgcacggttc aagcaaaggt
     6661 acccagatgg tatcatctct aaacctaaga atttagacta caaagacgat gacgataaag
     6721 ggggcggtgg aggttaccca tacgatgttc cagattacgc tgctgagggc agaggaagtc
     6781 ttctaacatg cggtgacgtg gaggagaatc ccggcccttc cgggatgacc gagtacaagc
     6841 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     6901 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     6961 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     7021 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     7081 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     7141 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     7201 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     7261 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     7321 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     7381 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaatctag gtcgacaatc
     7441 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     7501 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7561 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7621 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7681 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7741 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7801 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     7861 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7921 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7981 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga
     8041 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8101 gaagggctaa ttcactccca acgatgtcaa gaattgtgaa agaccccacc tgtaggtttg
     8161 gcaagttagc ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga
     8221 gaagttcaga tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct
     8281 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc
     8341 cgccctcagc agtttctagc gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
     8401 tgaccctgtg ccttatttga actaaccaat cagtttgctt cttgcttctg tttgtgtgct
     8461 tctgctccct gagctcaata aaagagccca caacccctca cttggtgggc cagtcctctg
     8521 atagactgtg tcccctggat acccgtatat cgattctgct ttttgcttct actgggtctc
     8581 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8641 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8701 ctggtaacta gagatccctc agaccctttc agtcagtgtg gaaaatctct agcagtagta
     8761 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8821 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8881 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8941 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     9001 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     9061 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9121 cctagacttt tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt
     9181 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9241 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9301 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     9361 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9421 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9481 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9541 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9601 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9661 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9721 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9781 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9841 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9901 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9961 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10021 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10081 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10141 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10201 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10261 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    10321 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    10381 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    10441 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    10501 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10561 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10621 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10681 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10741 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10801 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    10861 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10921 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10981 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11041 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11101 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    11161 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    11221 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    11281 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
    11341 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
    11401 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    11461 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
    11521 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    11581 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
    11641 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    11701 gtgctgca
//
