LOCUS       sequence-347144-        9096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sgRNA for CasRx in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Xia Z, Tang M, Ma J, Zhang H, Gimple RC, Prager BC, Tang H, Sun C, 
            Liu F, Lin P, Mei Y, Du R, Rich JN, Xie Q
  TITLE     Epitranscriptomic editing of the RNA N6-methyladenosine modification
            by dCasRx conjugated methyltransferase and demethylase.
  JOURNAL   Nucleic Acids Res. 2021 Jul 21;49(13):7361-7374. doi: 
            10.1093/nar/gkab517.
  PUBMED    34181729
REFERENCE   2  (bases 1 to 9096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2021-07-21- 21"; volume: "49"; issue: 
            "13"; pages: "7361-7374"
COMMENT     Sequence Label: lenti-sgRNA-BSD
FEATURES             Location/Qualifiers
     source          1..9096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(111..130)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        306..685
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        687..885
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     840..860
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             903..1083
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1130..1255
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1748..1981
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2166..2210
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2508..2625
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2676..2924
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2676..2696
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2847..2866
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2929..2958
                     /label=RfxCas13d DR30
                     /note="5' processed 30-base direct repeat for an RfxCas13d 
                     gRNA (Konermann et al., 2018)"
     promoter        3602..3813
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3832..3841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3838..4236
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    4252..4840
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4305..4325)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4723..4734)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4912..5145
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5171..5188)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5177..5401
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5447..5875
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5534..5553)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5744..5765
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5884..5904)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5889..6218
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6069..6204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6131..6150
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6266..6313
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6332..6706
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6836..6957
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6873..6892)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6927..6946
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7006..7022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7006..7022)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7019..7041)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7030..7046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7054..7060,7061..7078,7079..7084))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7099..7120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7237..7254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7408..7996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7488..7507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8167..8958,8959..9027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8790..8809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9028..9096,1..36))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
       61 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
      121 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
      181 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
      241 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgc
      301 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      361 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      421 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      481 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      541 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      601 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      661 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      721 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      781 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      841 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta
      901 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      961 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     1021 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     1081 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     1141 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     1201 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     1261 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     1321 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     1381 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     1441 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     1501 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     1561 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     1621 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     1681 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     1741 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     1801 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     1861 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     1921 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     1981 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     2041 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     2101 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     2161 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     2221 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     2281 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     2341 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     2401 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     2461 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     2521 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2581 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     2641 gacagcagag atccagtttg gttaattaag gtaccgaggg cctatttccc atgattcctt
     2701 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa
     2761 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
     2821 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
     2881 tcgatttctt ggctttatat atcttgtgga aaggacgaaa aaacaccgaa cccctaccaa
     2941 ctggtcgggg tttgaaaccg agacggttgt aaatgagcac acaaaataca catgctaaaa
     3001 tattatattc tatgaccttt ataaaatcaa ccaaaatctt ctttttaata actttagtat
     3061 caataattag aatttttatg ttcctttttg caaactttta ataaaaatga gcaaaataaa
     3121 aaaacgctag ttttagtaac tcgcgttgtt ttcttcacct ttaataatag ctactccacc
     3181 acttgttcct aagcggtcag ctcctgcttc aatcattttt tgagcatctt cctaatctta
     3241 caaaatgacc atcatgtttt attgccattc caaatctgtc agcatctggg tcattcataa
     3301 taataatatc tgcatcatgt ttaataccat attcaagcgg tatttttcat gcaggatcaa
     3361 attctggatt tggatttaca acatttttaa atgtttcatc ttcaaatgca tgctcttcaa
     3421 cctcaataac gttatatcct gattcacgta atatttttgg ggtaaattta gttcctgttc
     3481 cattaactgc gctaaaaata atttttaaat cttttttagc ttcttgctct tttttgtacg
     3541 tctctttttt ttgctagcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     3601 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     3661 tccggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     3721 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     3781 ctttttcgca acgggtttgc cgccagaaca caggaccggt tctagagcgc tgccaccatg
     3841 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
     3901 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
     3961 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
     4021 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
     4081 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
     4141 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
     4201 gaattgctgc cctctggtta tgtgtgggag ggctaaacgc gttaagtcga caatcaacct
     4261 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4321 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4381 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4441 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4501 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4561 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4621 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4681 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4741 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4801 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     4861 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     4921 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     4981 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5041 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5101 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
     5161 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5221 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5281 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     5341 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     5401 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     5461 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     5521 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     5581 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     5641 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     5701 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     5761 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5821 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     5881 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5941 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6001 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6061 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6121 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     6181 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     6241 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     6301 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     6361 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     6421 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     6481 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     6541 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     6601 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     6661 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     6721 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6781 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6841 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6901 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6961 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7021 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7081 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7141 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7201 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7261 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7321 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7381 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7441 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7501 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7561 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7621 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7681 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7741 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7801 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7861 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7921 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7981 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8041 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8101 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8161 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8221 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8281 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8341 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8401 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8461 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8521 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8581 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8641 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8701 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8761 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8821 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8881 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8941 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9001 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9061 tcagggttat tgtctcatga gcggatacat atttga
//
