LOCUS       sequence-347145-       11216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCasRx-ALKBH5 in mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11216)
  AUTHORS   Xia Z, Tang M, Ma J, Zhang H, Gimple RC, Prager BC, Tang H, Sun C, 
            Liu F, Lin P, Mei Y, Du R, Rich JN, Xie Q
  TITLE     Epitranscriptomic editing of the RNA N6-methyladenosine modification
            by dCasRx conjugated methyltransferase and demethylase.
  JOURNAL   Nucleic Acids Res. 2021 Jul 21;49(13):7361-7374. doi: 
            10.1093/nar/gkab517.
  PUBMED    34181729
REFERENCE   2  (bases 1 to 11216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2021-07-21- 21"; volume: "49"; issue: 
            "13"; pages: "7361-7374"
COMMENT     Sequence Label: pMSCV-dCasRx-ALKBH5-PURO
FEATURES             Location/Qualifiers
     source          1..11216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..122
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     114..131
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     115..131
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        146..372
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             373..553
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    600..725
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1218..1451
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1636..1680
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1947..2063
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             2096..2116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2126..5023
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLAHWVVANNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFANKAVALEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             5033..5080
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      5088..5097
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6272..6295
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6311..6337
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6341..6394
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6401..7000
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6401..6420)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6897..6917
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7013..7601
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7066..7086)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7484..7495)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8150..8330
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8402..8523
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8439..8458)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8493..8512
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8563..8698
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8731..8747)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8731..8747)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8744..8766)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8755..8771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8779..8785,8786..8803,8804..8809))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8824..8845
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8962..8979)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9133..9721)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9892..10683,10684..10752))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10515..10534
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10753..10857)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10925..10943
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10981..11003)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11103..11122
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
       61 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
      121 cgacggccag tgccaagctg acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac
      181 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
      241 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
      301 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
      361 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
      421 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
      481 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      541 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
      601 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
      661 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
      721 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
      781 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
      841 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
      901 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
      961 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     1021 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccact gatcttcaga
     1081 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     1141 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     1201 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     1261 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     1321 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     1381 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     1441 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     1501 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     1561 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     1621 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     1681 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     1741 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     1801 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     1861 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     1921 aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt
     1981 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2041 caaattacaa aattcaaaat tttatcgata ctagagctag agctagcgca tgagccccaa
     2101 gaagaagaga aaggtggagg ccagcatcga aaaaaaaaag tccttcgcca agggcatggg
     2161 cgtgaagtcc acactcgtgt ccggctccaa agtgtacatg acaaccttcg ccgaaggcag
     2221 cgacgccagg ctggaaaaga tcgtggaggg cgacagcatc aggagcgtga atgagggcga
     2281 ggccttcagc gctgaaatgg ccgataaaaa cgccggctat aagatcggca acgccaaatt
     2341 cagccatcct aagggctacg ccgtggtggc taacaaccct ctgtatacag gacccgtcca
     2401 gcaggatatg ctcggcctga aggaaactct ggaaaagagg tacttcggcg agagcgctga
     2461 tggcaatgac aatatttgta tccaggtgat ccataacatc ctggacattg aaaaaatcct
     2521 cgccgaatac attaccaacg ccgcctacgc cgtcaacaat atctccggcc tggataagga
     2581 cattattgga ttcggcaagt tctccacagt gtatacctac gacgaattca aagaccccga
     2641 gcaccatagg gccgctttca acaataacga taagctcatc aacgccatca aggcccagta
     2701 tgacgagttc gacaacttcc tcgataaccc cagactcggc tatttcggcc aggccttttt
     2761 cagcaaggag ggcagaaatt acatcatcaa ttacggcaac gaatgctatg acattctggc
     2821 cctcctgagc ggactggcgc actgggtggt cgctaacaac gaagaagagt ccaggatctc
     2881 caggacctgg ctctacaacc tcgataagaa cctcgacaac gaatacatct ccaccctcaa
     2941 ctacctctac gacaggatca ccaatgagct gaccaactcc ttctccaaga actccgccgc
     3001 caacgtgaac tatattgccg aaactctggg aatcaaccct gccgaattcg ccgaacaata
     3061 tttcagattc agcattatga aagagcagaa aaacctcgga ttcaatatca ccaagctcag
     3121 ggaagtgatg ctggacagga aggatatgtc cgagatcagg aaaaatcata aggtgttcga
     3181 ctccatcagg accaaggtct acaccatgat ggactttgtg atttataggt attacatcga
     3241 agaggatgcc aaggtggctg ccgccaataa gtccctcccc gataatgaga agtccctgag
     3301 cgagaaggat atctttgtga ttaacctgag gggctccttc aacgacgacc agaaggatgc
     3361 cctctactac gatgaagcta atagaatttg gagaaagctc gaaaatatca tgcacaacat
     3421 caaggaattt aggggaaaca agacaagaga gtataagaag aaggacgccc ctagactgcc
     3481 cagaatcctg cccgctggcc gtgatgtttc cgccttcagc aaactcatgt atgccctgac
     3541 catgttcctg gatggcaagg agatcaacga cctcctgacc accctgatta ataaattcga
     3601 taacatccag agcttcctga aggtgatgcc tctcatcgga gtcaacgcta agttcgtgga
     3661 ggaatacgcc tttttcaaag actccgccaa gatcgccgat gagctgaggc tgatcaagtc
     3721 cttcgctaga atgggagaac ctattgccga tgccaggagg gccatgtata tcgacgccat
     3781 ccgtatttta ggaaccaacc tgtcctatga tgagctcaag gccctcgccg acaccttttc
     3841 cctggacgag aacggaaaca agctcaagaa aggcaagcac ggcatgagaa atttcattat
     3901 taataacgtg atcagcaata aaaggttcca ctacctgatc agatacggtg atcctgccca
     3961 cctccatgag atcgccaaaa acgaggccgt ggtgaagttc gtgctcggca ggatcgctga
     4021 catccagaaa aaacagggcc agaacggcaa gaaccagatc gacaggtact acgaaacttg
     4081 tatcggaaag gataagggca agagcgtgag cgaaaaggtg gacgctctca caaagatcat
     4141 caccggaatg aactacgacc aattcgacaa gaaaaggagc gtcattgagg acaccggcag
     4201 ggaaaacgcc gagagggaga agtttaaaaa gatcatcagc ctgtacctca ccgtgatcta
     4261 ccacatcctc aagaatattg tcaatatcaa cgccaggtac gtcatcggat tccattgcgt
     4321 cgagcgtgat gctcaactgt acaaggagaa aggctacgac atcaatctca agaaactgga
     4381 agagaaggga ttcagctccg tcaccaagct ctgcgctggc attgatgaaa ctgcccccga
     4441 taagagaaag gacgtggaaa aggagatggc tgaaagagcc aaggagagca ttgacagcct
     4501 cgagagcgcc aaccccaagc tgtatgccaa ttacatcaaa tacagcgacg agaagaaagc
     4561 cgaggagttc accaggcaga ttaacaggga gaaggccaaa accgccctga acgcctacct
     4621 gaggaacacc aagtggaatg tgatcatcag ggaggacctc ctgagaattg acaacaagac
     4681 atgtaccctg ttcgcaaaca aggccgtcgc cctggaagtg gccaggtatg tccacgccta
     4741 tatcaacgac attgccgagg tcaattccta cttccaactg taccattaca tcatgcagag
     4801 aattatcatg aatgagaggt acgagaaaag cagcggaaag gtgtccgagt acttcgacgc
     4861 tgtgaatgac gagaagaagt acaacgatag gctcctgaaa ctgctgtgtg tgcctttcgg
     4921 ctactgtatc cccaggttta agaacctgag catcgaggcc ctgttcgata ggaacgaggc
     4981 cgccaagttc gacaaggaga aaaagaaggt gtccggcaat tccggatccg gaaagcgacc
     5041 tgccgccaca aagaaggctg gacaggctaa gaagaagaaa ggtagttcca tggcggccgc
     5101 cagcggctac acggacctgc gtgagaagct caagtccatg acgtcccggg acaactataa
     5161 ggcgggcagc cgggaggccg ccgccgctgc cgcagccgcc gtagccgccg cagccgcagc
     5221 cgccgctgcc gccgaacctt accctgtgtc cggggccaag cgcaagtatc aggaggactc
     5281 ggaccccgag cgcagcgact atgaggagca gcagctgcag aaggaggagg aggcgcgcaa
     5341 ggtgaagagc ggcatccgcc agatgcgcct cttcagccag gacgagtgcg ccaagatcga
     5401 ggcccgcatt gacgaggtgg tgtcccgcgc tgagaagggc ctgtacaacg agcacacggt
     5461 ggaccgggcc ccactgcgca acaagtactt cttcggcgaa ggctacactt acggcgccca
     5521 gctgcagaag cgcgggcccg gccaggagcg cctctacccg ccgggcgacg tggacgagat
     5581 ccccgagtgg gtgcaccagc tggtgatcca aaagctggtg gagcaccgcg tcatccccga
     5641 gggcttcgtc aacagcgccg tcatcaacga ctaccagccc ggcggctgca tcgtgtctca
     5701 cgtggacccc atccacatct tcgagcgccc catcgtgtcc gtgtccttct ttagcgactc
     5761 tgcgctgtgc ttcggctgca agttccagtt caagcctatt cgggtgtcgg aaccagtgct
     5821 ttccctgccg gtgcgcaggg gaagcgtgac tgtgctcagt ggatatgctg ctgatgaaat
     5881 cactcactgc atacggcctc aggacatcaa ggagcgccga gcagtcatca tcctcaggaa
     5941 gacaagatta gatgcacccc ggttggaaac aaagtccctg agcagctccg tgttaccacc
     6001 cagctatgct tcagatcgcc tgtcaggaaa caacagggac cctgctctga aacccaagcg
     6061 gtcccaccgc aaggcagacc ctgatgctgc ccacaggcca cggatcctgg agatggacaa
     6121 ggaagagaac cggcgctcgg tgctgctgcc cacacaccgg cggaggggta gcttcagctc
     6181 tgagaactac tggcgcaagt catacgagtc ctcagaggac tgctctgagg cagcaggcag
     6241 ccctgcccga aaggtgaaga tgcggcggca cgactacaaa gacgatgacg ataaaggggg
     6301 cggtggaggt tacccatacg atgttccaga ttacgctgct gagggcagag gaagtcttct
     6361 aacatgcggt gacgtggagg agaatcccgg cccttccggg atgaccgagt acaagcccac
     6421 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     6481 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     6541 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6601 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     6661 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     6721 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     6781 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     6841 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     6901 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     6961 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga atctaggtcg acaatcaacc
     7021 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7081 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     7141 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7201 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7261 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7321 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7381 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     7441 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7501 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7561 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa
     7621 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7681 ggctaattca ctcccaacga tgtcaagaat tgtgaaagac cccacctgta ggtttggcaa
     7741 gttagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag
     7801 ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg
     7861 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc
     7921 ctcagcagtt tctagcgaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     7981 cctgtgcctt atttgaacta accaatcagt ttgcttcttg cttctgtttg tgtgcttctg
     8041 ctccctgagc tcaataaaag agcccacaac ccctcacttg gtgggccagt cctctgatag
     8101 actgtgtccc ctggataccc gtatatcgat tctgcttttt gcttctactg ggtctctctg
     8161 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8221 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8281 taactagaga tccctcagac cctttcagtc agtgtggaaa atctctagca gtagtagttc
     8341 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8401 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8461 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8521 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     8581 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8641 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8701 gacttttgca gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     8761 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8821 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8881 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8941 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     9001 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     9061 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     9121 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9181 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9241 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9301 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9361 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9421 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9481 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9541 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     9601 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9661 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9721 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9781 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9841 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9901 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9961 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10021 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    10081 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    10141 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10201 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10261 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10321 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10381 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10441 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10501 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10561 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10621 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10681 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10741 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10801 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10861 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatgt cattaaccta
    10921 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
    10981 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    11041 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
    11101 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    11161 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaact
//
