LOCUS       sequence-347147-       13476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector for doxycycline-inducible PLXNB2 overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13476)
  AUTHORS   Junqueira Alves C, Dariolli R, Haydak J, Kang S, Hannah T, Wiener 
            RJ, DeFronzo S, Tejero R, Gusella GL, Ramakrishnan A, Alves Dias R, 
            Wojcinski A, Kesari S, Shen L, Sobie EA, Rodrigues Furtado de 
            Mendonca JP, Azeloglu EU, Zou H, Friedel RH
  TITLE     Plexin-B2 orchestrates collective stem cell dynamics via actomyosin 
            contractility, cytoskeletal tension and adhesion.
  JOURNAL   Nat Commun. 2021 Oct 14;12(1):6019. doi: 10.1038/s41467-021-26296-7.
  PUBMED    34650052
REFERENCE   2  (bases 1 to 13476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-10-14"; volume: "12(1):6019. doi"; pages: " 
            10.1038/s41467-021-26296-7"
COMMENT     Sequence Label: pLV-CMVtight-PLXNB2 iOE
FEATURES             Location/Qualifiers
     source          1..13476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4137
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4159..4473
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    4167..4185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4203..4221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4238..4256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4274..4292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4310..4328
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4345..4363
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4381..4399
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4446..4470
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4551..4575
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10120..10144)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10175..10763
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10228..10248)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10646..10657)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        10806..11305
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10841..10864)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11238..11257
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11319..12122
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11382..11401)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11992..12011
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             12213..12446
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12518..12639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12555..12574)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12609..12628
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12679..12814
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12834..12853)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12835..12853)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12863..12880)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12863..12879)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12872..12894)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      13021..13449
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13108..13127)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13318..13339
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc
     4141 gatcacgaga ctagcctcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
     4201 actccctatc agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt
     4261 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
     4321 gatagagaac gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac
     4381 tccctatcag tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata
     4441 agcagagctc gtttagtgaa ccgtcagatc gcctggagaa ttcgagctcg gtacccgggg
     4501 atccaccggt tcgtcgacta gtccagtgtg gtggaattct gcagatatca acaagtttgt
     4561 acaaaaaagc agaagggccg tcaaggccca ccatggcact gcagctctgg gccctgaccc
     4621 tgctgggcct gctgggcgca ggtgccagcc tgaggccccg caagctggac ttcttccgca
     4681 gcgagaaaga gctgaaccac ctggctgtgg atgaggcctc aggcgtggtg tacctggggg
     4741 cggtgaatgc cctctaccag ctggatgcga agctgcagct ggagcagcag gtggccacgg
     4801 gcccggccct ggacaacaag aagtgcacgc cgcccatcga ggccagccag tgccatgagg
     4861 ctgagatgac tgacaatgtc aaccagctgc tgctgctcga ccctcccagg aagcgcctgg
     4921 tggagtgcgg cagcctcttc aagggcatct gcgctctgcg cgccctgagc aacatctccc
     4981 tccgcctgtt ctacgaggac ggcagcgggg agaagtcttt cgtggccagc aatgatgagg
     5041 gcgtggccac agtggggctg gtgagctcca cgggtcctgg tggtgaccgc gtgctgtttg
     5101 tgggcaaagg caatgggcca cacgacaacg gcatcatcgt gagcactcgg ctgttggacc
     5161 ggactgacag cagggaggcc tttgaagcct acacggacca cgccacctac aaggccggct
     5221 acctgtccac caacacacag cagttcgtgg cggccttcga ggacggcccc tacgtcttct
     5281 ttgtcttcaa ccagcaggac aagcacccgg cccggaaccg cacgctgctg gcacgcatgt
     5341 gcagagaaga ccccaactac tactcctacc tggagatgga cctgcagtgc cgggaccccg
     5401 acatccacgc cgctgccttt ggcacctgcc tggccgcctc cgtggctgcg cctggctctg
     5461 gcagggtgct atatgctgtc ttcagcagag acagccggag cagtgggggg cccggtgcgg
     5521 gcctctgcct gttcccgctg gacaaggtgc acgccaagat ggaggccaac cgcaacgcct
     5581 gttacacagg cacccgggag gcccgtgaca tcttctacaa gcccttccac ggcgatatcc
     5641 agtgcggcgg ccacgcgccg ggctccagca agagcttccc atgtggctcg gagcacctgc
     5701 cctacccgct gggcagccgc gacgggctca gaggcacagc cgtgctgcag cgtggaggcc
     5761 tgaacctcac ggccgtgacg gtcgccgccg agaacaacca cactgttgct tttctgggca
     5821 cctctgatgg ccggatcctc aaggtgtacc tcaccccaga tggcacctcc tcagagtacg
     5881 actctatcct tgtggagata aacaagagag tcaagcgcga cctggtactg tctggagacc
     5941 tgggcagcct gtacgccatg acccaggaca aggtgttccg gctgccggtg caggagtgcc
     6001 tgagctaccc gacctgcacc cagtgccgcg actcccagga cccctactgc ggctggtgcg
     6061 tcgtcgaggg acgatgcacc cggaaggccg agtgtccgcg ggccgaggag gccagccact
     6121 ggctgtggag ccgaagcaag tcctgcgtgg ccgtcaccag cgcccagcca cagaacatga
     6181 gccggcgggc ccagggggag gtgcagctga ccgtcagccc cctccctgcc ctgagcgagg
     6241 aggacgagtt gctgtgcctt tttggggagt cgccgccaca ccccgcccgc gtggagggcg
     6301 aggccgtcat ctgcaactcc ccaagcagca tccccgtcac accgccaggc caggaccacg
     6361 tggccgtgac catccagctc ctccttagac gaggcaacat cttcctcacg tcctaccagt
     6421 accccttcta cgactgccgc caggccatga gcctggagga gaacctgccg tgcatctcct
     6481 gcgtgagcaa ccgctggacc tgccagtggg acctgcgcta ccacgagtgc cgggaggctt
     6541 cgcccaaccc tgaggacggc atcgtccgtg cccacatgga ggacagctgt ccccagttcc
     6601 tgggacccag ccccctggtg atccccatga accacgagac agatgtgaac ttccagggca
     6661 agaacctgga caccgtgaag ggttcctccc tgcacgtggg cagtgacttg ctcaagttca
     6721 tggagccggt gaccatgcag gaatctggga ccttcgcctt tcggacccca aagctgtccc
     6781 acgatgccaa cgagacgctg cccctgcacc tctacgtcaa gtcttacggc aagaatatcg
     6841 acagcaagct ccatgtgacc ctctacaact gctcctttgg ccgcagcgac tgcagcctgt
     6901 gccgggccgc taaccccgac tacaggtgtg cgtggtgcgg gggccagagc aggtgcgtgt
     6961 atgaggccct gtgcaacacc acctccgagt gcccgccgcc cgtcatcacc aggatccagc
     7021 ctgagacggg ccccctgggt gggggcatcc gcatcaccat cctggggtcc aatttgggcg
     7081 tccaagcagg ggacatccag aggatctctg tggccggccg gaactgctcc tttcagccgg
     7141 aacgttactc cgtgtccacc cggatcgtgt gtgtgatcga ggctgcggag acgcctttca
     7201 cggggggtgt cgaggtggac gtcttcggga aactgggccg ttcgcctccc aatgtccagt
     7261 tcaccttcca acagcccaag cctctcagtg tggagccgca gcagggaccg caggcgggcg
     7321 gcaccacact gaccatccac ggcacccacc tggacacggg ctcccaggag gacgtgcggg
     7381 tgaccctcaa cggcgtcccg tgtaaagtga cgaagtttgg ggcgcagctc cagtgtgtca
     7441 ctggccccca ggcgacacgg ggccagatgc ttctggaggt ctcctacggg gggtcccccg
     7501 tgcccaaccc cggcatcttc ttcacctacc gcgaaaaccc cgtactgcga gccttcgagc
     7561 cgctacgaag ctttgccagt ggtggccgca gcatcaacgt cacgggtcag ggcttcagcc
     7621 tgatccagag gtttgccatg gtggtcatcg cggagcccct gcagtcctgg cagccgccgc
     7681 gggaggctga atccctgcag cccatgacgg tggtgggtac agactacgtg ttccacaatg
     7741 acaccaaggt cgtcttcctg tccccggctg tgcctgagga gccagaggcc tacaacctca
     7801 cggtgctgat cgagatggac gggcaccgtg ccctgctcag aacagaggcc ggggccttcg
     7861 agtacgtgcc tgaccccacc tttgagaact tcacaggtgg cgtcaagaag caggtcaaca
     7921 agctcatcca cgcccggggc accaatctga acaaggcgat gacgctgcag gaggccgagg
     7981 ccttcgtggg tgccgagcgc tgcaccatga agacgctgac ggagaccgac ctgtactgtg
     8041 agcccccgga ggtgcagccc ccgcccaagc ggcggcagaa acgagacacc acacacaacc
     8101 tgcccgagtt cattgtgaag ttcggctctc gcgagtgggt gctgggccgc gtggagtacg
     8161 acacacgggt gagcgacgtg ccgctcagcc tcatcttgcc gctggtcatc gtgcccatgg
     8221 tggtcgtcat cgcggtgtct gtctactgct actggaggaa gagccagcag gccgaacgag
     8281 agtatgagaa gatcaagtcc cagctggagg gcctggagga gagcgtgcgg gaccgctgca
     8341 agaaggaatt cacagacctg atgatcgaga tggaggacca gaccaacgac gtgcacgagg
     8401 ccggcatccc cgtgctggac tacaagacct acaccgaccg cgtcttcttc ctgccctcca
     8461 aggacggcga caaggacgtg atgatcaccg gcaagctgga catccctgag ccgcggcggc
     8521 cggtggtgga gcaggccctc taccagttct ccaacctgct gaacagcaag tctttcctca
     8581 tcaatttcat ccacaccctg gagaaccagc gggagttctc ggcccgcgcc aaggtctact
     8641 tcgcgtccct gctgacggtg gcgctgcacg ggaaactgga gtactacacg gacatcatgc
     8701 acacgctctt cctggagctc ctggagcagt acgtggtggc caagaacccc aagctgatgc
     8761 tgcgcaggtc tgagactgtg gtggagagga tgctgtccaa ctggatgtcc atctgcctgt
     8821 accagtacct caaggacagt gccggggagc ccctgtacaa gctcttcaag gccatcaaac
     8881 atcaggtgga aaagggcccg gtggatgcgg tacagaagaa ggccaagtac actctcaacg
     8941 acacggggct gctgggggat gatgtggagt acgcacccct gacggtgagc gtgatcgtgc
     9001 aggacgaggg agtggacgcc atcccggtga aggtcctcaa ctgtgacacc atctcccagg
     9061 tcaaggagaa gatcattgac caggtgtacc gtgggcagcc ctgctcctgc tggcccaggc
     9121 cagacagcgt ggtcctggag tggcgtccgg gctccacagc gcagatcctg tcggacctgg
     9181 acctgacgtc acagcgggag ggccggtgga agcgcgtcaa cacccttatg cactacaatg
     9241 tccgggatgg agccaccctc atcctgtcca aggtgggggt ctcccagcag ccggaggaca
     9301 gccagcagga cctgcctggg gagcgccatg ccctcctgga ggaggagaac cgggtgtggc
     9361 acctggtgcg gccgaccgac gaggtggacg agggcaagtc caagagaggc agcgtgaaag
     9421 agaaggagcg gacgaaggcc atcaccgaga tctacctgac gcggctgctc tcagtcaagg
     9481 gcacactgca gcagtttgtg gacaacttct tccagagcgt gctggcgcct gggcacgcgg
     9541 tgccacctgc agtcaagtac ttcttcgact tcctggacga gcaggcagag aagcacaaca
     9601 tccaggatga agacaccatc cacatctgga agacgaacag cttaccgctc cggttctggg
     9661 tgaacatcct caagaacccc cacttcatct ttgacgtgca tgtccacgag gtggtggacg
     9721 cctcgctgtc agtcatcgcg cagaccttca tggatgcctg cacgcgcacg gagcataagc
     9781 tgagccgcga ttctcccagc aacaagctgc tgtacgccaa ggagatctcc acctacaaga
     9841 agatggtgga ggattactac aaggggatcc ggcagatggt gcaggtcagc gaccaggaca
     9901 tgaacacaca cctggcagag atttcccggg cgcacacgga ctccttgaac accctcgtgg
     9961 cactccacca gctctaccaa tacacgcaga agtactatga cgagatcatc aatgccttgg
    10021 aggaggatcc tgccgcccag aagatgcagc tggccttccg cctgcagcag attgccgctg
    10081 cactggagaa caaggtcact gacctctagg gcctcatggg cccagctttc ttgtacaaag
    10141 tggttgatat ccagcacagt ggcggccgct cgacaatcaa cctctggatt acaaaatttg
    10201 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
    10261 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
    10321 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
    10381 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
    10441 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
    10501 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
    10561 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg
    10621 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
    10681 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
    10741 ctccctttgg gccgcctccc cgcctggaat tctgcagata tccggactag tgatctaatt
    10801 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
    10861 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
    10921 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
    10981 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
    11041 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
    11101 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
    11161 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
    11221 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
    11281 ctcttcctca tctccgggcc tttcgacctg cagccaatat gggatcggcc attgaacaag
    11341 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
    11401 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
    11461 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
    11521 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
    11581 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
    11641 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
    11701 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
    11761 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
    11821 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
    11881 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
    11941 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
    12001 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
    12061 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
    12121 gaggggatcc gtcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga
    12181 tcttagccac tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag
    12241 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
    12301 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    12361 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    12421 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
    12481 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
    12541 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    12601 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
    12661 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    12721 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
    12781 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
    12841 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
    12901 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
    12961 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
    13021 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
    13081 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    13141 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    13201 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
    13261 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
    13321 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
    13381 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
    13441 acgcgaattt taacaaaata ttaacgctta caattt
//
