LOCUS       sequence-347164-       10089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector for doxycycline-inducible YAP1 overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Junqueira Alves C, Dariolli R, Haydak J, Kang S, Hannah T, Wiener 
            RJ, DeFronzo S, Tejero R, Gusella GL, Ramakrishnan A, Alves Dias R, 
            Wojcinski A, Kesari S, Shen L, Sobie EA, Rodrigues Furtado de 
            Mendonca JP, Azeloglu EU, Zou H, Friedel RH
  TITLE     Plexin-B2 orchestrates collective stem cell dynamics via actomyosin 
            contractility, cytoskeletal tension and adhesion.
  JOURNAL   Nat Commun. 2021 Oct 14;12(1):6019. doi: 10.1038/s41467-021-26296-7.
  PUBMED    34650052
REFERENCE   2  (bases 1 to 10089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-10-14"; volume: "12(1):6019. doi"; pages: " 
            10.1038/s41467-021-26296-7"
COMMENT     Sequence Label: pLV-TRE3G-hYAP1-CMV-mCherry-T2A-Puro
FEATURES             Location/Qualifiers
     source          1..10089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1964..1982
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2000..2018
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2036..2054
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2072..2090
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2108..2126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2144..2162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2180..2198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2243..2267
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2334..2358
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2359..2368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3892..3916)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3934..4522
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3987..4007)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4405..4416)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     enhancer        4552..4931
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4932..5135
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5086..5106
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      5162..5171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5168..5875
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5313..5331)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5581..5600
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5885..5938
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5939..6538
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5939..5958)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6435..6455
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6610..6843
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6915..7036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6952..6971)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7006..7025
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7076..7211
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7231..7250)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7232..7250)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7260..7277)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7260..7276)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7269..7291)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7418..7873
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7505..7524)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7715..7736
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7899..8003
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8004..8072,8073..8864)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8222..8241)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9035..9623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9524..9543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9777..9794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9911..9932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9947..9952,9953..9970,9971..9977)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9985..10001
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9990..10012
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10009..10025
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10009..10025
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10044..10064
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10046..10064
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgtt tactccctat cagtgataga
     1981 gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct
     2041 atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc
     2101 agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga
     2161 gaacgtatat ccagtttact ccctatcagt gatagagaac gtataagctt taggcgtgta
     2221 cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt
     2281 ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg taaacaagtt
     2341 tgtacaaaaa agcaggctgc caccatggat cccgggcagc agccgccgcc tcaaccggcc
     2401 ccccagggcc aagggcagcc gccttcgcag cccccgcagg ggcagggccc gccgtccgga
     2461 cccgggcaac cggcacccgc ggcgacccag gcggcgccgc aggcaccccc cgccgggcat
     2521 cagatcgtgc acgtccgcgg ggactcggag accgacctgg aggcgctctt caacgccgtc
     2581 atgaacccca agacggccaa cgtgccccag accgtgccca tgaggctccg gaagctgccc
     2641 gactccttct tcaagccgcc ggagcccaaa tcccactccc gacaggccag tactgatgca
     2701 ggcactgcag gagccctgac tccacagcat gttcgagctc attcctctcc agcttctctg
     2761 cagttgggag ctgtttctcc tgggacactg acccccactg gagtagtctc tggcccagca
     2821 gctacaccca cagctcagca tcttcgacag tcttcttttg agatacctga tgatgtacct
     2881 ctgccagcag gttgggagat ggcaaagaca tcttctggtc agagatactt cttaaatcac
     2941 atcgatcaga caacaacatg gcaggacccc aggaaggcca tgctgtccca gatgaacgtc
     3001 acagccccca ccagtccacc agtgcagcag aatatgatga actcggcttc aggtcctctt
     3061 cctgatggat gggaacaagc catgactcag gatggagaaa tttactatat aaaccataag
     3121 aacaagacca cctcttggct agacccaagg cttgaccctc gttttgccat gaaccagaga
     3181 atcagtcaga gtgctccagt gaaacagcca ccacccctgg ctccccagag cccacaggga
     3241 ggcgtcatgg gtggcagcaa ctccaaccag cagcaacaga tgcgactgca gcaactgcag
     3301 atggagaagg agaggctgcg gctgaaacag caagaactgc ttcggcaggt gaggccacag
     3361 gcaatgcgga atatcaatcc cagcacagca aattctccaa aatgtcagga gttagccctg
     3421 cgtagccagt taccaacact ggagcaggat ggtgggactc aaaatccagt gtcttctccc
     3481 gggatgtctc aggaattgag aacaatgacg accaatagct cagatccttt ccttaacagt
     3541 ggcacctatc actctcgaga tgagagtaca gacagtggac taagcatgag cagctacagt
     3601 gtccctcgaa ccccagatga cttcctgaac agtgtggatg agatggatac aggtgatact
     3661 atcaaccaaa gcaccctgcc ctcacagcag aaccgtttcc cagactacct tgaagccatt
     3721 cctgggacaa atgtggacct tggaacactg gaaggagatg gaatgaacat agaaggagag
     3781 gagctgatgc caagtctgca ggaagctttg agttctgaca tccttaatga catggagtct
     3841 gttttggctg ccaccaagct agataaagaa agctttctta catggttata gacccagctt
     3901 tcttgtacaa agtggtgata atcgaattcc gataatcaac ctctggatta caaaatttgt
     3961 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4021 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4081 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4141 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4201 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4261 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4321 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     4381 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4441 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4501 tccctttggg ccgcctcccc gcatcgggaa ttcccgcggt tcgaacgcgt tgacattgat
     4561 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4621 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4681 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4741 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4801 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4861 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     4921 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     4981 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     5041 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     5101 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
     5161 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     5221 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     5281 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     5341 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     5401 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     5461 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     5521 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     5581 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     5641 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     5701 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     5761 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     5821 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggctc
     5881 cggagagggc aggggaagtc ttctaacatg cggggacgtg gaggaaaatc ccggccccat
     5941 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     6001 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     6061 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6121 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6181 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     6241 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     6301 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     6361 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6421 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6481 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagg
     6541 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6601 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     6661 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6721 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6781 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6841 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     6901 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6961 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7021 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     7081 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7141 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7201 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     7261 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7321 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7381 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     7441 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7501 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7561 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7621 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7681 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7741 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7801 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     7861 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7921 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7981 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     8041 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8101 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8161 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8221 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     8281 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     8341 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     8401 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8461 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     8521 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     8581 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     8641 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     8701 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     8761 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     8821 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8881 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8941 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9001 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9061 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9121 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9421 gtgagctatg agaaagcgcc acgcttcccg aagagagaaa ggcggacagg tatccggtaa
     9481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     9541 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     9601 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     9661 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     9721 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     9781 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     9841 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     9901 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     9961 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    10021 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
    10081 tgcaagctt
//
