LOCUS       sequence-347187-       10338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC2D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10338)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 10338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-eGFP-TAX1BP1 CC2D
FEATURES             Location/Qualifiers
     source          1..10338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     217..236
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             308..706
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    748..869
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(785..804)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    895..1483
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(948..968)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1366..1377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1558..1791
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(1910..1928)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1996..2100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2101..2169,2170..2961)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2319..2338)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3132..3720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3621..3640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3874..3891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4131..4152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4167..4172,4173..4190,4191..4197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4205..4221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4210..4232
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4229..4245
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4229..4245
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             4506..5139
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5186..5311
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5808..6041
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6226..6270
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6563..6680
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6732..7035
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7036..7239
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7190..7210
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7236..7260
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      7319..7328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7325..7327,7328..7330,7331..8041)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7325..7327,7328..7330,7331..8041)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7370..7391)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7631..7650)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7978..7999
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    8057..8081
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10080..10104)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        join(10123..10338,1..284)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10158..10181)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
       61 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
      121 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
      181 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
      241 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccattaa
      301 ttaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
      361 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
      421 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
      481 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
      541 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
      601 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
      661 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac ttcgtggccg
      721 aggagcagga ctgacactcg acctcgaaac ttgtttattg cagcttataa tggttacaaa
      781 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
      841 ggtttgtcca aactcatcaa tgtatcttat catgtctatg catcgataga tcctaatcaa
      901 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
      961 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     1021 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     1081 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     1141 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     1201 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     1261 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     1321 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     1381 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     1441 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac
     1501 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     1561 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     1621 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     1681 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     1741 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     1801 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     1861 ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata
     1921 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1981 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2041 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2101 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2161 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2221 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2281 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2341 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2401 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2461 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2521 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2581 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2641 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     2701 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     2761 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2821 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2881 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2941 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3001 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3061 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3241 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3781 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3841 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3901 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
     3961 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     4021 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
     4081 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     4141 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     4201 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
     4261 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     4321 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     4381 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     4441 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     4501 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     4561 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     4621 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     4681 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     4741 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     4801 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     4861 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     4921 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     4981 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5041 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     5101 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     5161 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     5221 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     5281 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     5341 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     5401 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     5461 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     5521 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     5581 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     5641 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     5701 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     5761 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     5821 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     5881 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     5941 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     6001 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     6061 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     6121 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     6181 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     6241 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     6301 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     6361 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     6421 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     6481 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccgaattcac
     6541 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     6601 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     6661 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggacta
     6721 gtggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     6781 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     6841 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     6901 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     6961 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     7021 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     7081 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     7141 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     7201 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     7261 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggcg gccgatctgc
     7321 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     7381 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     7441 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     7501 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     7561 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     7621 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     7681 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     7741 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     7801 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     7861 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     7921 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     7981 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     8041 ggctcgaggt tatcaaacaa gtttgtacaa aaaagttggc atgacatcct ttcaagaagt
     8101 cccattgcag acttccaact ttgcccatgt catctttcaa aatgtggcca agagttacct
     8161 tcctaatgca cacctggaat gtcattacac cttaactcca tatattcatc cacatccaaa
     8221 agattgggtt ggtatattca aggttggatg gagtactgct cgtgattatt acacgttttt
     8281 atggtcccct atgcctgaac attatgtgga aggatcaaca gtcaattgtg tactagcatt
     8341 ccaaggatat taccttccaa atgatgatgg agaattttat cagttctgtt acgttaccca
     8401 taagggtgaa attcgtggag caagtacacc tttccagttt cgagcttctt ctccagttga
     8461 agagctgctt actatggaag atgaaggaaa ttctgacatg ttagtggtga ccacaaaagc
     8521 aggccttctt gagttgaaaa ttgagaaaac catgaaagaa aaagaagaac tgttaaagtt
     8581 aattgccgtt ctggaaaaag aaacagcaca acttcgagaa caagttggga gaatggaaag
     8641 agaacttaac catgagaaag aaagatgtga ccaactgcaa gcagaacaaa agggtcttac
     8701 tgaagtaaca caaagcttaa aaatggaaaa tgaagagttt aagaagaggt tcagtgatgc
     8761 tacatccaaa gcccatcagc ttgaggaaga tattgtgtca gtaacacata aagcaattga
     8821 aaaagaaacc gaattagaca gtttaaagga caaactcaag aaggcacaac atgaaagaga
     8881 acaacttgaa tgtcagttga agacagagaa ggatgaaaag gaactttata aggtacattt
     8941 gaagaataca gaaatagaaa ataccaagct tatgtcagag gtccagactt taaaaaattt
     9001 agatgggaac aaagaaagcg tgattactca tttcaaagaa gagattggca ggctgcagtt
     9061 atgtttggct gaaaaggaaa atctgcaaag aactttcctg cttacaacct caagtaaaga
     9121 agatactaat aataatgtct tcacaaagaa aacggggaat cagcagaaag tgaatgatgc
     9181 ttcagtaaac acagacccag ccacttctgc ctctactgta gatgtaaagc catcaccttc
     9241 tgcagcagag gcagattttg acatagtaac aaaggggcaa gtctgtgaaa tgaccaaaga
     9301 aattgctgac aaaacagaaa agtataataa atgtaaacaa ctcttgcagg atgagaaagc
     9361 aaaatgcaat aaatatgctg atgaacttgc aaaaatggag ctgaaatgga aagaacaagt
     9421 gaaaattgct gaaaatgtaa aacttgaact agctgaagta caggacaatt ataaagaact
     9481 taaaaggagt ctagaaaatc cagcagaaag gaaaatggaa ggtcagaatt cccagagtcc
     9541 tcaatgtttc aaaacatgct cagagcaaaa tggttatgtt ctcacattgt caaatgcaca
     9601 accagttctg caatatggta atccttatgc atctcaggaa acaagagatg gagcagatgg
     9661 tgctttttac ccagatgaaa tacaaaggcc acctgtcaga gtcccctctt ggggactgga
     9721 agacaatgtt gtctgcagcc agcctgctcg aaactttagt cggcctgatg gcttagagga
     9781 ctctgaggat agcaaagaag atgagaatgt gcctactgct cctgatcctc caagtcaaca
     9841 tttacgtggg catgggacag gcttttgctt tgattccagc tttgatgttc acaagaagtg
     9901 tcccctctgt gagttaatgt ttcctcctaa ctatgatcag agcaaatttg aagaacatgt
     9961 tgaaagtcac tggaaggtgt gcccgatgtg cagcgagcag ttccctcctg actatgacca
    10021 gcaggtgttt gaaaggcatg tgcagaccca ttttgatcag aatgttctaa attttgacta
    10081 cccaactttc ttgtacaaag tggttcgata acgaattcta ccgggtaggg gaggcgcttt
    10141 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
    10201 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
    10261 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
    10321 cgcagctcgc gtcgtgca
//
