LOCUS       sequence-347188-        8888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the DisCas7-11 protein only for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Ozcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, 
            Koonin EV, Abudayyeh OO, Gootenberg JS
  TITLE     Programmable RNA targeting with the single-protein CRISPR effector 
            Cas7-11.
  JOURNAL   Nature. 2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5.
  PUBMED    34489594
REFERENCE   2  (bases 1 to 8888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 
            10.1038/s41586-021-03886-5."
COMMENT     Sequence Label: pDF0039 DisCas7-11 only expression with CRISPR...
FEATURES             Location/Qualifiers
     source          1..8888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2715..3509)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(2715..3509)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2826..2845)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3436..3455
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3510..3614)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3738..3755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3872..4417)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3952..3971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaggagatga taagcggatt tcccgtttga tgaaccccgc ttttgatgaa acagatgtgg
       61 ctgtgccgga aaagccgaaa accgatgccg aggttcggat tgaggcggaa aaggtttatt
      121 atcctcatta ttttgtcgag cctcataaaa aggtggagcg tgaagaaaag ccttgcggcc
      181 atcagaaatt tcatgaagga cggctgaccg gaaaaattcg atgcaaactg atcacaaaga
      241 cgccgctcat tgtaccggac acctcgaatg acgatttttt caggcccgca gacaaagaag
      301 ccagaaaaga aaaagatgag tatcacaaaa gctatgcctt tttcaggctt cacaagcaaa
      361 ttatgattcc cggttccgag ctgcggggca tggtcagttc ggtttacgag accgtgacca
      421 actcctgctt tcgcattttt gatgagacca agcggctttc gtggcggatg gatgcagacc
      481 atcagaatgt gcttcaagat tttcttcccg gcagagttac ggctgacgga aagcatatac
      541 aaaaattttc tgaaaccgca cgggttcctt tttatgataa aacccagaag cattttgaca
      601 tattggatga acaggaaata gcaggcgaaa aacctgttag aatgtgggtc aaaagattta
      661 tcaaacgttt gtctctggtc gatcctgcta aacatcctca aaaaaagcag gacaacaaat
      721 ggaagcgcag aaaagaggga atagccacat tcatagaaca gaaaaacggc agttattatt
      781 tcaatgtagt tacgaacaat ggctgtacga gctttcattt atggcacaaa cctgacaact
      841 ttgaccaaga gaaacttgaa ggtatccaga atggtgagaa attggattgt tgggtgagag
      901 attccagata tcaaaaggct ttccaggaaa tacccgaaaa tgatccggat ggatgggaat
      961 gtaaagaagg ataccttcat gttgtcggtc ccagcaaagt agagttttcc gacaagaaag
     1021 gtgatgtcat aaacaatttt cagggaaccc tgccatctgt accgaatgat tggaaaacga
     1081 tccgaaccaa tgatttcaaa aaccgaaaac gcaaaaatga acccgtattt tgctgtgagg
     1141 atgataaggg aaactattat acgatggcaa aatattgtga aacctttttc tttgacctca
     1201 aagaaaatga ggagtatgaa attccagaaa aagcccgtat taaatacaaa gagctgttga
     1261 gagtttataa caataatccc caagcagttc ctgaatcggt atttcaatct cgcgtcgcaa
     1321 gagaaaatgt cgaaaagctt aaatctggcg atcttgtcta ttttaaacac aatgaaaaat
     1381 atgttgagga tattgttccc gtccgcattt cccggacagt tgatgatcgg atgatcggca
     1441 aacgcatgtc agccgatctc cgcccctgtc acggtgactg ggtggaagac ggcgaccttt
     1501 ccgccctgaa cgcctacccg gaaaagcgcc ttctcctgcg tcatcccaaa ggattgtgcc
     1561 cggcctgccg ccttttcgga accggttcat acaagggccg ggttcggttc ggatttgcaa
     1621 gccttgaaaa tgatcccgaa tggctgatac ccggcaagaa tcccggtgat cccttccacg
     1681 ggggaccggt aatgctgtcg cttctggagc gcccccggcc cacatggtcg atccccggat
     1741 cggacaataa gttcaaagtg ccgggccgga agttttatgt ccatcatcat gcatggaaaa
     1801 cgataaaaga cggcaaccat cccacaaccg gaaaggccat agaacaaagc cccaacaacc
     1861 ggacagttga agccttggcc gggggaaaca gcttttcttt tgaaatcgct tttgaaaatc
     1921 tgaaagaatg ggaactcggt ttgctgattc attccctgca attggaaaaa gggttggcac
     1981 acaagctggg catggcaaaa tcaatgggat tcggcagcgt tgagattgat gttgaatctg
     2041 tgcgtctgag gaaggactgg aaacagtgga gaaacggaaa cagtgaaata ccaaactggc
     2101 ttgggaaagg ctttgccaag ctaaaggaat ggttcagaga tgagctggat tttatagaaa
     2161 atctgaagaa gttgctctgg tttccagaag gagaccaagc gccgagagtc tgttatccga
     2221 tgctgagaaa aaaggatgac ccgaacggaa actccggcta cgaagaactg aaagacggcg
     2281 aatttaaaaa ggaagaccgc cagaaaaagc tcaccacgcc ttggacccct tgggcctgat
     2341 cttctgtgcc gcctgatact ctcccttcgt aagatgattc agcatgaaaa gttcattatt
     2401 tcagcccgga agttcatttt cgggctgaaa cctgttggtt tcctccgata acccgcttca
     2461 gcgggtttca ataactgagc cggaccgggt cgaatttgct ttcgaatttc tgccattcat
     2521 ccgcttatta tcacttattc aggcgtagca accaggcgtt taagggcacg tctgacgctc
     2581 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2641 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2701 cttggtctga cagttcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     2761 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     2821 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     2881 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     2941 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac
     3001 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     3061 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     3121 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     3181 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     3241 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     3301 agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc
     3361 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     3421 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     3481 cctgcgtgca atccatcttg ttcaatcata ctcttccttt ttcaatatta ttgaagcatt
     3541 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3601 ataggggttc cgcgcacgta tactggctta ctatgttggc actgatgagg gtgtcagtga
     3661 agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac
     3721 aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga
     3781 gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa gatacttaac
     3841 agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc
     3901 atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc
     3961 aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
     4021 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
     4081 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
     4141 gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg
     4201 gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg
     4261 ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa
     4321 agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc
     4381 agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat
     4441 aaaatatttc tagacaggtt gactcggagc ggagatgggc gtaatgtcag tgagaatttc
     4501 gtttcgggtc gttttcggcc ctgttctttt aaaaaatcct ttttatacag catgatacac
     4561 gcatgaatgg cgcgttttcc catgctgccc accccgagga tttttcattt tttaagacta
     4621 ccggctttcc ggcttatggt tttgaattgt ttgactaaac cggcttttcc gttttttacc
     4681 cgaaatcggt atttccaatg aaatcactca ggttggaaag ccggttttct ttgatgtcac
     4741 ggaaccgcca atataacaat acaggtatta tagttgggtt ggaaagccgg ttttctttga
     4801 tgtcacggaa cattgtaaac tgcatatctg tgtttgccgt ctggttggaa agccggtttt
     4861 ctttgatgtc acggaactca cgtgagtcag tcttagttct atacttcttt agggttggaa
     4921 agccggtttt ctttgatgtc acggaacagg aacttgaaca acatcgttac taacgagctg
     4981 gttggaaagc cggttttctt tgatgtcacg gaacctctag ataattcgat ttggtccggt
     5041 ttgggggttg gaaagccggt tttctttgat gtcacggaac aattctatcg cttacggacc
     5101 tacaaatgca actggttgga aagccggttt tctttgatgt cacggaactt tgtatctatc
     5161 atatcgctcg gaagactaaa ggttggaaag ccggttttct ttgatgtcac ggaacgagag
     5221 atggtgtttg tctggtagag catcaaggtt ggaaagccgg ttttctttga tgtcacggaa
     5281 cacatcaaga ctcagtatca caaaatgttc taaaggttgg aaagccggtt ttctttgatg
     5341 tcacggaaca cattatgatc aatggtgctt tcacgaattt ttcggttgga aagccggttt
     5401 tctttgatgt cacggaactc agctcgtcta cttgtgggat gccttgactg ggttggaaag
     5461 ccggttttct ttgatgtcac ggaactttct tgatcaaaaa tacctttata gagtatcctg
     5521 gttggaaagc cggttttctt tgatgtcacg gaacttacga ctactttctt ctcttatctg
     5581 tttatttggt tggaaagccg gttttctttg atgtcacgga acaccagatg gcgggtttac
     5641 gtcaaacggg ttaggttgga aagccggttt tctttgatgt cacggaacga gaccaattat
     5701 tcgcgcagtt gcacacatca gtggttggaa agccggtttt ctttgatgtc acggaaccaa
     5761 acacgttctt cataatgaac caataatgtg gttggaaagc cggttttctt tgatgtcacg
     5821 gaacattttg cgctctcctt ctgttattcg ggttaggttg gaaagccggt tttctttgat
     5881 gtcacggaac tatttctcgt atctttcagg atctccggca gaaggttgga aagccggttt
     5941 tctttgatgt cacggaaccg tagcggacag cgatgttttc tccgactgcc tggttggaaa
     6001 gccggttttc tttgatgtca cggaactttt atctttttct tatcaaaaaa gctcttcggt
     6061 tggaaagccg gttttctttg atgtcacgga actcgtttga ttttcgcagt ctgcctcata
     6121 atacggttgg aaagccggtt ttctttgatg tcacggaacc actgttttcg agacgtaaaa
     6181 ttatttttga ggttggaaag ccggttttct ttgatgtcac ggttgccacc cggatcctca
     6241 gagaccgggc agcccacacg tcttccttct ttcccgatcc agcagagcta cagggaaagc
     6301 gtataagaac gggaaaaatt caggccggag cgggactttc cttttctcac acattttccc
     6361 tgtagttttc ccaaggcttt gagggacgat ttccacgcga cataccgagg agacacatac
     6421 catgacgacg actatgaaaa tttccatcga atttctcgaa ccctttcgga tgactaaatg
     6481 gcaggaaagt accagacgaa acaaaaacaa caaagaattc gtccggggtc aggcatttgc
     6541 cagatggcac agaaacaaaa aagacaacac aaagggccgt ccctatatca ccggaaccct
     6601 gctccgttcg gcagtcatcc gctcggcgga gaacctgctg acgctttccg atggaaaaat
     6661 ttcagaaaag acctgttgtc cggggaaatt cgacactgaa gacaaagaca gattgttaca
     6721 attgcgccag cgttccacgc tcaggtggac agataaaaat ccctgcccgg acaacgccga
     6781 aacatattgc ccgttctgtg aactgctggg ccgatccggt aacgacggaa agaaagccga
     6841 aaaaaaagat tggagatttc gtatccattt cggcaatctc agtttgcctg gaaaaccgga
     6901 ctttgacggc ccgaaggcaa tcggatcaca gcgcgttctg aaccgggtcg atttcaaaag
     6961 cggcaaggcc catgactttt ttaaagccta tgaagtggat catacccgct ttccccggtt
     7021 tgagggagag atcaccattg acaacaaggt gagtgccgag gcccgaaagc ttctgtgcga
     7081 cagcctgaaa ttcacagacc ggctctgcgg ggccttgtgt gtcatccggt ttgacgagta
     7141 tactccggct gccgattccg gcaaacagac ggagaatgtg caggcagagc caaatgccaa
     7201 tctcgccgag aaaaccgccg agcagatcat cagcatcttg gatgacaaca aaaaaaccga
     7261 atacacccgc cttctggctg atgccatccg ttcgctccgc cggagcagca agcttgtcgc
     7321 cggtctgccc aaagatcacg atggaaagga tgatcattat ctctgggaca tcggaaagaa
     7381 aaaaaaagat gaaaacagcg taacaatccg ccaaatcctg acaacatcgg cggatacaaa
     7441 agaacttaaa aatgccggaa agtggcgcga attctgcgag aagctgggcg aggcactcta
     7501 cctgaaatcc aaagacatga gcggcggctt gaaaataacc cggcgaattc tgggagacgc
     7561 cgaatttcac ggaaagccgg atcgtctgga aaaatcccgt tctgtttcca tcggctctgt
     7621 gctgaaagag acggttgtct gcggagaact ggtcgcaaaa acgccgtttt tcttcggagc
     7681 cattgatgag gatgcaaaac agaccgatct tcaggtgttg ctgacgcccg acaacaaata
     7741 ccgcctgccg cgctccgctg ttcggggaat cctgcgccgg gatttgcaga catactttga
     7801 cagcccgtgc aatgccgaac tcggcggaag gccctgtatg tgcaaaacct gccggatcat
     7861 gcgcggtatc accgtcatgg atgcccgcag tgaatacaat gcgccgccgg aaatccgcca
     7921 ccgcacccgc atcaatccct ttaccggcac ggttgccgag ggagcgctgt tcaatatgga
     7981 agtggcaccg gagggaattg tgtttccctt ccagcttcgc taccggggaa gcgaagacgg
     8041 attgccggat gcactgaaaa ccgtcctgaa atggtgggca gagggccagg cgtttatgag
     8101 cggcgcggca tccacgggga aagggcgatt ccgtatggaa aacgccaagt atgagacatt
     8161 ggatttgtcc gatgaaaatc aacggaatga ttatctgaaa aattggggat ggcgggatga
     8221 aaaaggattg gaggaactga aaaaacgcct gaattctggc ttgccggaac cgggcaatta
     8281 ccgtgacccc aagtggcacg aaatcaacgt cagtatagaa atggcctccc ccttcatcaa
     8341 tggcgacccg atccgcgccg ccgtggataa aaggggtacg gatgtggtca cgttcgtcaa
     8401 atacaaagcc gaaggtgaag aggccaaacc agtttgtgcc tacaaggcgg agagtttcag
     8461 gggcgtgata cgttcggcag ttgcgcgaat ccatatggaa gatggtgttc cgctgaccga
     8521 actgacccac agcgattgcg aatgcctgct ctgccagatt ttcggcagcg aatatgaggc
     8581 cgggaaaatc cggtttgagg atctggtgtt tgaatcagat ccagagccgg tgacattcga
     8641 ccatgtggcc attgaccggt ttaccggcgg cgcggctgac aaaaagaaat ttgacgattc
     8701 tccgcttccg ggcagtccgg ccagacctct catgctaaaa ggcagcttct ggatacggcg
     8761 ggatgttctg gaagatgagg aatattgcaa agcgctggga aaggcgctgg cagatgtgaa
     8821 caacgggctt tatcctctgg gcggaaaaag cgccatcgga tatgggcagg taaaaagtct
     8881 ggggatta
//
