LOCUS       sequence-347234-       13185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pmyo-3 DAF-2 unc-54 3' UTR C.elegans muscle expression of DAF-2 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 13185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH4733
FEATURES             Location/Qualifiers
     source          1..13185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10313..10330)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10313..10329)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10322..10344)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10542..10997
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10629..10648)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10839..10860
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11014..11033)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11133..11155
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11193..11211)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11279..11383
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11384..11452,11453..12244)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11602..11621)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12415..13003
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12904..12923
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13157..13174
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgaatg
      241 gccatgggac gtcgacagct tgcatgcctg cagcgtttcc gataccccct tatatcagca
      301 cacattcagt catcatacca caaaatgttg tgatagtgat aggcaaaact accgtatttc
      361 ctctattaat cttgcatgca agactaattt tcgattaacc cgtaggggtg caagactaat
      421 agagactgca agactattag aggctgaaat actaattttc gtatgctcaa taattttgga
      481 aattggccta ttttttgtag aaacttgata ccgtttgcgg ccgccggcta taataagttc
      541 ttgaataaaa taattttccc gacaaaacat gagtatttct ttcgaaaata aaagtgcagg
      601 ctaattagag attattctgt aattaactgc ataatttgtc acgtgccata gttttacatt
      661 ccactacgtc atagttctta aaatactaat ctcctgaaaa tagaagtagg tgaagaaagt
      721 ttaattatca gttctaaaat gacaattgat ctttggaata tgttctgaaa ctaccgatca
      781 ttgaacagat gctatttgaa tgatatagaa ttgtatattt gcaatttctg aaacgcgttc
      841 ttaaaggcac acagattaat tcaaaagggt ctggccgcaa aaaggtttat ggtggccgat
      901 tttgagtttt gtgtgtgatt gctttttcac aatcagtgtt ttcaggatta tgtgatgaac
      961 tagatcttca agtttcgtta catttcatat gttttcggaa ctcacgaagt acatattggg
     1021 tattgtgctc aaaaaattca gcaatcagct tcgctccgct gactttagaa cccaaaaaaa
     1081 tagtatggcc aaactgactg tgttacgatc atttcaattt ttcaatacat atttaagatt
     1141 tctaagagta agaaggtcaa aaactgttct ggaatacata tatatttttc aggttacaat
     1201 tagtcaaaaa gtgcactgaa atatacgttt taatttcacg aataacccaa ttagttcaat
     1261 gtatttttgg tcaaccaacg ttaaagtttg gcttccaacc aattatcatt tctgatcaac
     1321 cacaatgttt tttctttatc tgcaagttaa ttttttattt ttatccagat gtttggcata
     1381 tttttcaatt cttcactagc gcccacttct tgcacttccg gcgccctgaa tctaatgcat
     1441 ctgttgcaag aattgaaaga ccaatcaaca cattgttttc ttcacgagat actgaagaaa
     1501 atgaataaaa acagagaaaa agagccatgt gattagtgac aactgttgct aacagatacc
     1561 atagcttgga cttggtacgt gatggcaacg tatgggtcaa caaaaatgat tgcagagggg
     1621 gtgcaaaaca gtcaagtcga gaaaatatga aaaacagaaa acaaagaaca gaaaaatggg
     1681 tttgagagtc agtataattt ataaaagaaa aattgtacat agaaattaac catttttgta
     1741 gaagaagtta tttttcaagc atcgttaaaa attattcaaa gcaccttatt tcatatttaa
     1801 ttttaaacat ggttaaatga acaacacggt gcgcaatcag gaaaacttga aatctgaaac
     1861 tgttgttgtg atcttcttcg caactgttca gatagcacta gtgtaatgtt aagagtgcgc
     1921 gaatataatg gaatataatg gatcacacct cctgccatca ggtaaacgtc tctgttatca
     1981 catatttcca actattaaat ttttaccttt tacagtttta catttttttg aaaaaagtaa
     2041 ctttttgtct tcaaaatccc tgacgaaaat atcaaatatt ttaatcgaga ctgcagagga
     2101 accgattgat gatttggaaa atccagcttt acctgtgtaa gaactgaaaa gtttcataac
     2161 cctagggtat tcccagttac attccccact ggctaacaat agcacccagt ttttcatcac
     2221 ctttcttcaa atttctcggc gatttgttaa aaacaaaatt tgtgtccctt ctctgatatc
     2281 tctatgtctc taaacacaag ttcatcggaa aacgaaggag ggtaggtgtt ggttgggctc
     2341 ccgaagtgaa aatagaagag caagaataga atattagaga gagagtgcag agagggcggg
     2401 atagctcccg ggattccgtt ttcttcttct ttatcttcaa cgatgatgtg tgtgcgtgtt
     2461 gtatagattc tgttgctccc ccacaactcg ctccgaaggc tcaatacaat tcaattgata
     2521 ttggaggaga gcctaccgga gtgggaggat aagaagaaac ataagaagaa gaagaagaag
     2581 aagcatgctt ctggtttttg atgctatgaa aacggcacaa aaagatgatt gaggtccctt
     2641 ttcaatacct tctctcatct ttcaaatccc attgaaacct aaaacttctc accacgcttt
     2701 accattgttc tccaaaaact tatagcaatg tctataactt ttttatctct gaaaagcagt
     2761 gttccatttt tctttttcct attttatttc aattgtttct cacatttcgt ttggattctt
     2821 tgcttgtcaa ccagcttctt cttccacttt taccgtctaa ttttcagggc agggagccat
     2881 caaacccacg accactagat ccatctagcc cgggatgaat attgtcagat gtcggagacg
     2941 acacaaaatt ttggaaaatt tggaagaaga gaatctcggc ccgagctgct cgtcgacgac
     3001 ttcaacaacc gctgccaccg aagctctcgg aacaaccact gaggatatga ggcttaagca
     3061 gcagcgaagc tcgtcgcgtg ccacggagca cgatattgtc gacggcaatc accacgacga
     3121 cgagcacatc acaatgagac ggcttcgact tgtcaaaaat tcgcggacgc ggcgtagaac
     3181 gacgcccgat tcaagtatgg actgctatga ggaaaacccg ccatcacaaa aaacttcaat
     3241 aaattattct tggatttcta aaaagtcatc aatgacgtca ttaatgcttt tactgctatt
     3301 cgcttttgta cagccgtgtg cctcaatagt cgaaaaacga tgcggcccaa tcgatattcg
     3361 aaataggccg tgggatatta agccgcaatg gtcgaaactt ggtgatccga acgaaaaaga
     3421 tttggctggt cagagaatgg tcaactgcac agtggtggaa ggttcgctga caatctcatt
     3481 tgtactgaaa cacaagacaa aagcacaaga agaaatgcat cgaagtctac agccaagata
     3541 ttcccaagac gaatttatca cttttccgca tctacgtgaa attactggaa ctctgctcgt
     3601 ttttgagact gaaggattag tggatttgcg taaaattttc ccaaatcttc gtgtaattgg
     3661 aggccgttcg ctgattcaac actatgcgct gataatttat cgaaatccgg atttggagat
     3721 cggtcttgac aagctttccg taattcgaaa tggtggtgta cggataatcg ataatcgaaa
     3781 actgtgctac acgaaaacga ttgattggaa acatttgatc acttcttcca tcaacgatgt
     3841 tgtcgttgat aatgctgccg agtacgctgt cactgagact ggattgatgt gcccacgtgg
     3901 agcttgcgaa gaggataaag gcgaatcaaa gtgtcattat ttggaggaaa agaatcagga
     3961 acaaggtgtc gaaagagttc agagttgttg gtcgaacacc acttgccaaa agtcttgtgc
     4021 ttatgatcgt cttcttccaa cgaaagaaat cggaccggga tgtgatgcga acggcgatcg
     4081 atgtcacgat caatgcgtgg gcggttgtga gcgtgtgaat gatgcgacag catgccacgc
     4141 gtgcaagaat gtctatcaca agggaaagtg tatcgaaaag tgtgatgctc acctgtacct
     4201 tctccttcaa cgtcgttgtg tgacccgtga gcagtgtctg cagctgaatc cggtgctctc
     4261 gaacaaaaca gtgcctatca aggcgacggc aggcctttgc tcggataaat gtcccgatgg
     4321 ttatcaaatc aacccggatg atcatcgaga atgccgaaaa tgcgttggca agtgtgagat
     4381 tgtgtgcgag atcaatcacg tcattgatac gtttccgaag gcacaggcga tcaggctatg
     4441 caatattatt gacggaaatc tgacgatcga gattcgcgga aaacaggatt cgggaatggc
     4501 gtccgagttg aaggatatat ttgcgaacat tcacacgatc accggctacc tgttggtacg
     4561 tcaatcgtca ccgtttatct cgttgaacat gttccggaat ttacgacgta ttgaggcaaa
     4621 gtcactgttc agaaatctat atgctatcac agtttttgaa aatccgaatt taaaaaagct
     4681 attcgattca acgacggatt tgacgcttga tcgtggaact gtgtcaattg ccaataacaa
     4741 gatgttatgc ttcaagtata tcaagcagct aatgtcaaag ttaaatatac cactcgatcc
     4801 gatagatcaa tcagaaggga caaatggtga gaaggcaatc tgtgaggata tggcaatcaa
     4861 cgtgagcatc acagcggtca acgcggactc ggtcttcttt agttggccct cattcaacat
     4921 taccgatata gatcagcgaa agtttctcgg ctacgagctc ttcttcaaag aagtcccacg
     4981 aatcgatgag aacatgacga tcgaagagga tcgaagtgcg tgtgtcgatt cgtggcagag
     5041 tgtcttcaaa cagtactacg agacgtcgaa cggtgaaccg accccggaca tttttatgga
     5101 tattggaccg cgcgagcgaa ttcggccgaa tacgctctac gcgtactatg tggcgacgca
     5161 gatggtgttg catgccggtg cgaagaacgg tgtatcgaag attggttttg tgaggacgag
     5221 ctactatacg cctgatcctc cgacgttggc actagcgcaa gtcgattcgg acgctattca
     5281 tattacgtgg gaagcgccgc tccaaccgaa cggagacctc acgcattaca caattatgtg
     5341 gcgtgagaat gaagtgagcc cgtacgagga agccgaaaag ttttgtacag atgcaagcac
     5401 ccccgcaaat cgacaacaca cgaaagatcc gaaagagacg attgtagccg ataagccagt
     5461 cgatattccg tcatcacgta ccgtagctcc gacacttttg actatgatgg gtcacgaaga
     5521 tcagcagaaa acgtgcgctg caacgcccgg ttgttgttcg tgttcggcta tcggagaatc
     5581 atcggaacag aacaagaaga agcgaccgga tccgatgtcg gcgatcgaat catctgcatt
     5641 tgagaataag ctgttggatg aggttttaat gccgagagac acgatgcgag tgagacgatc
     5701 aattgaagac gcgaatcgag tcagtgaaga gttggaaaaa gctgaaaatt tgggaaaagc
     5761 tccaaaaact ctcggtggaa agaagccgct gatccatatt tcgaagaaga agccgtcgag
     5821 cagcagcacc acatccacac cggctccaac gatcgcatca atgtatgcct taacaaggaa
     5881 accgactacg gtgccgggaa caaggattcg gctctacgag atctacgaac ctttacccgg
     5941 aagctgggcg attaatgtat cagctctggc attggataat agttatgtga tacgaaattt
     6001 gaagcattac acactttatg cgatttctct atccgcgtgc caaaacatga cagtacccgg
     6061 agcatcttgc tcaatatccc atcgtgcggg agcattgaaa cgaacaaaac acatcacaga
     6121 cattgataaa gtgttgaatg aaacaattga atggagattt atgaataata gtcaacaagt
     6181 caacgtgacg tgggatccac cgactgaagt gaatggtgga atattcggtt atgttgtaaa
     6241 gcttaagtca aaagtcgatg gatcaattgt tatgacgaga tgtgtcggtg cgaagagagg
     6301 atattcaaca cggaatcagg gtgtcctatt ccagaatttg gccgatggac gttattttgt
     6361 ctcagtaacg gcgacctctg tacacggcgc tggaccggaa gccgaatcct ccgacccaat
     6421 cgtcgtcatg acgccaggct tcttcactgt ggaaatcatt ctcggcatgc ttctcgtctt
     6481 tttgatttta atgtcaattg ccggttgtat aatctactac tacattcaag tacgctacgg
     6541 caaaaaagtg aaagctctat ctgactttat gcaattgaat cccgaatatt gtgtggacaa
     6601 taagtacaat gcagacgatt gggagctacg gcaggatgat gttgtgctcg gacaacagtg
     6661 tggagaggga tcattcggaa aagtgtacct cggaactgga aataatgttg tttctctgat
     6721 gggtgatcgt ttcggaccgt gtgctattaa gattaatgta gatgatccag cgtcgactga
     6781 gaatctcaac tatctcatgg aagctaatat tatgaagaac tttaagacta actttatcgt
     6841 caaactgtac ggagttatct ctactgtaca accagcgatg gttgtgatgg aaatgatgga
     6901 tcttggaaat ctccgtgact atctccgatc gaaacgcgaa gacgaagtgt tcaatgagac
     6961 ggactgcaac tttttcgaca taatcccgag ggataaattc catgagtggg ccgcacagat
     7021 ttgtgatggt atggcgtacc tggagtcgct caagttttgc catcgagatc tcgccgcacg
     7081 taattgcatg ataaatcggg atgagactgt caagattgga gatttcggaa tggctcgtga
     7141 tctattctat catgactatt ataagccatc gggcaagcgt atgatgcctg ttcgatggat
     7201 gtcacccgag tcgttgaaag acggaaagtt tgactcgaaa tctgatgttt ggagcttcgg
     7261 agttgttctc tatgaaatgg ttacactcgg tgctcagcca tatattggtt tgagtaatga
     7321 tgaggtgttg aattatattg gaatggcccg gaaggttatc aagaagcccg aatgttgtga
     7381 aaactattgg tataaggtga tgaaaatgtg ctggagatac tcacctcggg atcgtccgac
     7441 gttcctccag ctcgttcatc ttctagcagc tgaagcttca ccagaattcc gagatttatc
     7501 atttgtccta accgataatc aaatgatcct tgacgattca gaagcactgg atcttgatga
     7561 tattgatgat actgatatga atgatcaggt tgtcgaggtg gcaccggatg ttgagaacgt
     7621 cgaggttcag agtgattcgg aacgtcggaa tacggattca ataccgttga aacagtttaa
     7681 gacgatccct ccgatcaatg cgacgacgag tcattcgaca atatcgattg atgagacacc
     7741 gatgaaagcg aagcagcgag aaggatcgct ggatgaggag tacgcattga tgaatcatag
     7801 tggaggtccg agtgatgcgg aagttcggac gtatgctggt gatggagatt atgtggagag
     7861 agatgttcga gagaatgatg tgccaacgcg acgaaatact ggtgcatcaa catcaagtta
     7921 cacaggtggt ggtccatatt gcctaacaaa tcgtggtggt tcaaatgaac gaggagccgg
     7981 tttcggtgaa gcagtacgat taactgatgg tgttggaagt ggacatttaa atgatgatga
     8041 ttatgttgaa aaagagatat catccatgga tacgcgccgg agcacgggcg cctcgagctc
     8101 ttcctacggt gttccacaga cgaattggag tggaaatcgt ggtgccacgt attatacgag
     8161 taaagctcaa caggcagcaa ctgcagcagc agcagcagca gcagctctcc aacagcaaca
     8221 aaatggtggt cgaggcgatc gattaactca actacccgga actggacatt tacaatcgac
     8281 acgtggtgga caagatggag attatattga aactgaaccg aaaaattata gaaataatgg
     8341 atctccatcg gtactggatt tttttttttg gagaaattag ccggaaaatt cgattttttt
     8401 cgctgaaaat ctgaaaaaat ttggctaaaa attgccctga aacacgtttt tttcggaatt
     8461 ttcatataaa aatggacatt ttcgtaaaaa attggctatg tgttgtgaat tttttctgac
     8521 ttttaaaggc ctttgaacta ggaatattaa aattttattg aaaattattg aaaagtgcaa
     8581 atttttattt tttttaggtg aaaaaagctt agatttaggc gaattttctg aaattttata
     8641 gtgaatttgg ttctcaggat attagccatt ttttcccgat tttttggtaa aaatttgtta
     8701 aaacccgctt caaatgactg tagttttata caaaaataac taaaaattcg atttttcaat
     8761 tattcagctc gaaaaagccc aacaaaaata tttaaaattt gaagaaaaaa gctaaaaatg
     8821 agtaaaaatg actggttttt ttcgcttttt ctatttgaat aaatttctga aaaatgacga
     8881 aaatctgaaa aaattacccc ggacattggg aaatttcgaa aaaatcttga aaatagtctg
     8941 aaatttttaa aatttacaga ttttcacaaa aaagggaaat ttcgcatttc ctgaaattgc
     9001 ccaaaaattt tttttttcat ttttttaaaa attctggaaa tttatttttg gctgtttcaa
     9061 atttttaaaa aaagaaaaat cccaaaaatt ggtgaaaact ccattaaaat tctccagaaa
     9121 attcaaattt tccatgaaaa attgttttaa aagcatgaaa atctgccgat ttttgtctaa
     9181 aatttttaaa aatatttcaa aaatttcaga aaaaacagga aaagcgaata ttacataaat
     9241 aaaaaatctg gaaattctag aaactgaaaa ttttttaatt tttccgctca aaaattctaa
     9301 tattcgatga aaatgagccc gaaaattcga gtaaatttcc aaaaaaaatt tttttttttt
     9361 tcaaacacaa atattaattt ttattaattt ttcagcgaaa cggcaacagc cgtgacattt
     9421 tcaacggacg ttcggctttc ggtgaaaatg agcatctaat cgaggataat gagcatccac
     9481 ttgtggtacc atggtattga tatctgagct ccgcatcggc cgctgtcatc agatcgccat
     9541 ctcgcgcccg tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt
     9601 tctccctgtg ctcccacccc ctatttttgt tattatcaaa aaaacttctt cttaatttct
     9661 ttgtttttta gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag
     9721 aattaattcg taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt
     9781 ctatcccaaa atctacacaa tgttctgtgt acacttctta tgtttttttt acttctgata
     9841 aatttttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt
     9901 ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat
     9961 catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt
    10021 atgaaattaa ttttcctgct tttgcttttt gggggtttcc cctattgttt gtcaagagtt
    10081 tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga
    10141 tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa
    10201 gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa
    10261 acataactgt ttcctactag tggccacgtg ggccgtgcac cttaagcttg gcactggccg
    10321 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
    10381 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
    10441 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
    10501 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
    10561 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
    10621 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
    10681 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
    10741 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    10801 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    10861 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
    10921 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
    10981 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
    11041 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
    11101 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
    11161 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
    11221 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
    11281 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    11341 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
    11401 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
    11461 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
    11521 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
    11581 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
    11641 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    11701 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    11761 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    11821 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    11881 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    11941 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    12001 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    12061 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    12121 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    12181 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    12241 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    12301 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    12361 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    12421 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    12481 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    12541 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    12601 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    12661 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    12721 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    12781 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    12841 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    12901 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    12961 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    13021 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    13081 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    13141 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaga
//
