LOCUS       sequence-347235-       12470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pges-1 DAF-2 unc-54 3' UTR C.elegans gut expression of  of DAF-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 12470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH4723
FEATURES             Location/Qualifiers
     source          1..12470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9598..9615)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9598..9614)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9607..9629)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9827..10282
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9914..9933)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10124..10145
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10299..10318)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10418..10440
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10478..10496)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10564..10668
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10669..10737,10738..11529)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10887..10906)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11700..12288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12189..12208
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12442..12459
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgaatg
      241 gccatgggac gtcgacgcat agcccgcagt cacttgaaag cacatttaag atggaatcaa
      301 atggtaagaa agctcgtgta atcaggagat tttatccggg gtatgccgag gaatatatgg
      361 tgcactcgca ttagctggca ttttttgtaa ttatcggatt tctacctttt tcttaatcta
      421 gtttgttatc attgtccatc atttacaacc aaaaatttgc gtcatttaca accaaaaatt
      481 gatctgacaa ttatcataat tcggttttta ttttcagaag aaacgctccg atgtgatcct
      541 atagtgaata tacttttcca tcctctctga aataaacaat tatgaagaat gcatcaattt
      601 gcattcgaaa taactgccgt attttgtcta ttggtatggc tgcagtacca tttttcttcg
      661 cgtctttgcg cagccttcgg gcgctaccaa taaggctaag ccggggtaag ttatttcaga
      721 attctaatca actaatctaa gtctcataca tcattgtcaa gtgacgcatt tttttaattt
      781 ctatgttaac tcgactttaa tattcttttc tggtttcacc agaaaaatgc tggactgaaa
      841 aattgaattt cccgcgattt gggaaaaacc gcagtttaag cttttggcat gaatacagtg
      901 aacacaggaa caaaaaattg acttgttatt ttgagtttat attaaatgtt aattttgcat
      961 ctcatatttt cacaaaactt gtttgcttga aaaatgtgca aataacaagt gaacatacat
     1021 caaggtttat gcagatcagc actgatgcaa ctgatagcaa aactgataag ggtcaaaatt
     1081 tcagtggcca gcacaaacac atacacattc gtagttcgtt ttgtgtttcg tgtatttagc
     1141 atttattttt caattccggc aatttgccga tttgccggaa attttgattt ttggcaaatt
     1201 gccagtttgc cgtttgccga acatcaattt gccgaaattt tttagaggga tctttataag
     1261 acggaaacac ttaaaattgt gcttttttgg aatttttttc aatgtttcaa tagaattagc
     1321 ttacatttaa aattagatgt aggaacgttt ataggacgct tacaattttg ccgattaaaa
     1381 tcgaagttct gaacttttca aaaaatgtgc aaaatttttt ttgacaaatt tgacaaatcg
     1441 gccggtttgt cgattgtccg gaatttttca gtttcggcga tttaccggtt tgccggaaaa
     1501 catcgtttgc cacccacaca cacataaaga gacgaaacaa cacaccatct tcaaaaaatt
     1561 tgtgaatctt catttccacg tggtttgcag ggtttcccat ccaccccact ctatctctcc
     1621 aagttgaatt ttggcaaaat tgaccacaca ccgatcaaaa actattgtgt gtgtgtgtat
     1681 ttgtgtcttc aataattcct gtctgtgtct cgcactatct attgactaca agaacgtgat
     1741 cgcagctcta cgaaaccttt actttatttt agaaatattt tttcatgcta ttagcaaaaa
     1801 aaaagttttt ttttgtctaa gtacacttaa aattttccaa aatttttaag atttttcaaa
     1861 atgtgcgatc aaagatctga caaaatgcca aaattttgaa gaggaaattt agtcggcttc
     1921 caaaattata agtggcagaa attgagcaat tgtcactttt tgacagtaaa tagaaaattt
     1981 tttgaaaaat tttgaaacgc tttattttgt tacttggtca ttttggcacc ataggagtag
     2041 tttttaacat tttccccact ggcgctactt cattttaaac ttttccaaag attttaattt
     2101 ttaattttaa aaaatttcat ataattcgtc tgcgtcttac agtttcaggc ttcaaaaatc
     2161 tattacatat cttatctttg aattcaggga tcccccggga tgaatattgt cagatgtcgg
     2221 agacgacaca aaattttgga aaatttggaa gaagagaatc tcggcccgag ctgctcgtcg
     2281 acgacttcaa caaccgctgc caccgaagct ctcggaacaa ccactgagga tatgaggctt
     2341 aagcagcagc gaagctcgtc gcgtgccacg gagcacgata ttgtcgacgg caatcaccac
     2401 gacgacgagc acatcacaat gagacggctt cgacttgtca aaaattcgcg gacgcggcgt
     2461 agaacgacgc ccgattcaag tatggactgc tatgaggaaa acccgccatc acaaaaaact
     2521 tcaataaatt attcttggat ttctaaaaag tcatcaatga cgtcattaat gcttttactg
     2581 ctattcgctt ttgtacagcc gtgtgcctca atagtcgaaa aacgatgcgg cccaatcgat
     2641 attcgaaata ggccgtggga tattaagccg caatggtcga aacttggtga tccgaacgaa
     2701 aaagatttgg ctggtcagag aatggtcaac tgcacagtgg tggaaggttc gctgacaatc
     2761 tcatttgtac tgaaacacaa gacaaaagca caagaagaaa tgcatcgaag tctacagcca
     2821 agatattccc aagacgaatt tatcactttt ccgcatctac gtgaaattac tggaactctg
     2881 ctcgtttttg agactgaagg attagtggat ttgcgtaaaa ttttcccaaa tcttcgtgta
     2941 attggaggcc gttcgctgat tcaacactat gcgctgataa tttatcgaaa tccggatttg
     3001 gagatcggtc ttgacaagct ttccgtaatt cgaaatggtg gtgtacggat aatcgataat
     3061 cgaaaactgt gctacacgaa aacgattgat tggaaacatt tgatcacttc ttccatcaac
     3121 gatgttgtcg ttgataatgc tgccgagtac gctgtcactg agactggatt gatgtgccca
     3181 cgtggagctt gcgaagagga taaaggcgaa tcaaagtgtc attatttgga ggaaaagaat
     3241 caggaacaag gtgtcgaaag agttcagagt tgttggtcga acaccacttg ccaaaagtct
     3301 tgtgcttatg atcgtcttct tccaacgaaa gaaatcggac cgggatgtga tgcgaacggc
     3361 gatcgatgtc acgatcaatg cgtgggcggt tgtgagcgtg tgaatgatgc gacagcatgc
     3421 cacgcgtgca agaatgtcta tcacaaggga aagtgtatcg aaaagtgtga tgctcacctg
     3481 taccttctcc ttcaacgtcg ttgtgtgacc cgtgagcagt gtctgcagct gaatccggtg
     3541 ctctcgaaca aaacagtgcc tatcaaggcg acggcaggcc tttgctcgga taaatgtccc
     3601 gatggttatc aaatcaaccc ggatgatcat cgagaatgcc gaaaatgcgt tggcaagtgt
     3661 gagattgtgt gcgagatcaa tcacgtcatt gatacgtttc cgaaggcaca ggcgatcagg
     3721 ctatgcaata ttattgacgg aaatctgacg atcgagattc gcggaaaaca ggattcggga
     3781 atggcgtccg agttgaagga tatatttgcg aacattcaca cgatcaccgg ctacctgttg
     3841 gtacgtcaat cgtcaccgtt tatctcgttg aacatgttcc ggaatttacg acgtattgag
     3901 gcaaagtcac tgttcagaaa tctatatgct atcacagttt ttgaaaatcc gaatttaaaa
     3961 aagctattcg attcaacgac ggatttgacg cttgatcgtg gaactgtgtc aattgccaat
     4021 aacaagatgt tatgcttcaa gtatatcaag cagctaatgt caaagttaaa tataccactc
     4081 gatccgatag atcaatcaga agggacaaat ggtgagaagg caatctgtga ggatatggca
     4141 atcaacgtga gcatcacagc ggtcaacgcg gactcggtct tctttagttg gccctcattc
     4201 aacattaccg atatagatca gcgaaagttt ctcggctacg agctcttctt caaagaagtc
     4261 ccacgaatcg atgagaacat gacgatcgaa gaggatcgaa gtgcgtgtgt cgattcgtgg
     4321 cagagtgtct tcaaacagta ctacgagacg tcgaacggtg aaccgacccc ggacattttt
     4381 atggatattg gaccgcgcga gcgaattcgg ccgaatacgc tctacgcgta ctatgtggcg
     4441 acgcagatgg tgttgcatgc cggtgcgaag aacggtgtat cgaagattgg ttttgtgagg
     4501 acgagctact atacgcctga tcctccgacg ttggcactag cgcaagtcga ttcggacgct
     4561 attcatatta cgtgggaagc gccgctccaa ccgaacggag acctcacgca ttacacaatt
     4621 atgtggcgtg agaatgaagt gagcccgtac gaggaagccg aaaagttttg tacagatgca
     4681 agcacccccg caaatcgaca acacacgaaa gatccgaaag agacgattgt agccgataag
     4741 ccagtcgata ttccgtcatc acgtaccgta gctccgacac ttttgactat gatgggtcac
     4801 gaagatcagc agaaaacgtg cgctgcaacg cccggttgtt gttcgtgttc ggctatcgga
     4861 gaatcatcgg aacagaacaa gaagaagcga ccggatccga tgtcggcgat cgaatcatct
     4921 gcatttgaga ataagctgtt ggatgaggtt ttaatgccga gagacacgat gcgagtgaga
     4981 cgatcaattg aagacgcgaa tcgagtcagt gaagagttgg aaaaagctga aaatttggga
     5041 aaagctccaa aaactctcgg tggaaagaag ccgctgatcc atatttcgaa gaagaagccg
     5101 tcgagcagca gcaccacatc cacaccggct ccaacgatcg catcaatgta tgccttaaca
     5161 aggaaaccga ctacggtgcc gggaacaagg attcggctct acgagatcta cgaaccttta
     5221 cccggaagct gggcgattaa tgtatcagct ctggcattgg ataatagtta tgtgatacga
     5281 aatttgaagc attacacact ttatgcgatt tctctatccg cgtgccaaaa catgacagta
     5341 cccggagcat cttgctcaat atcccatcgt gcgggagcat tgaaacgaac aaaacacatc
     5401 acagacattg ataaagtgtt gaatgaaaca attgaatgga gatttatgaa taatagtcaa
     5461 caagtcaacg tgacgtggga tccaccgact gaagtgaatg gtggaatatt cggttatgtt
     5521 gtaaagctta agtcaaaagt cgatggatca attgttatga cgagatgtgt cggtgcgaag
     5581 agaggatatt caacacggaa tcagggtgtc ctattccaga atttggccga tggacgttat
     5641 tttgtctcag taacggcgac ctctgtacac ggcgctggac cggaagccga atcctccgac
     5701 ccaatcgtcg tcatgacgcc aggcttcttc actgtggaaa tcattctcgg catgcttctc
     5761 gtctttttga ttttaatgtc aattgccggt tgtataatct actactacat tcaagtacgc
     5821 tacggcaaaa aagtgaaagc tctatctgac tttatgcaat tgaatcccga atattgtgtg
     5881 gacaataagt acaatgcaga cgattgggag ctacggcagg atgatgttgt gctcggacaa
     5941 cagtgtggag agggatcatt cggaaaagtg tacctcggaa ctggaaataa tgttgtttct
     6001 ctgatgggtg atcgtttcgg accgtgtgct attaagatta atgtagatga tccagcgtcg
     6061 actgagaatc tcaactatct catggaagct aatattatga agaactttaa gactaacttt
     6121 atcgtcaaac tgtacggagt tatctctact gtacaaccag cgatggttgt gatggaaatg
     6181 atggatcttg gaaatctccg tgactatctc cgatcgaaac gcgaagacga agtgttcaat
     6241 gagacggact gcaacttttt cgacataatc ccgagggata aattccatga gtgggccgca
     6301 cagatttgtg atggtatggc gtacctggag tcgctcaagt tttgccatcg agatctcgcc
     6361 gcacgtaatt gcatgataaa tcgggatgag actgtcaaga ttggagattt cggaatggct
     6421 cgtgatctat tctatcatga ctattataag ccatcgggca agcgtatgat gcctgttcga
     6481 tggatgtcac ccgagtcgtt gaaagacgga aagtttgact cgaaatctga tgtttggagc
     6541 ttcggagttg ttctctatga aatggttaca ctcggtgctc agccatatat tggtttgagt
     6601 aatgatgagg tgttgaatta tattggaatg gcccggaagg ttatcaagaa gcccgaatgt
     6661 tgtgaaaact attggtataa ggtgatgaaa atgtgctgga gatactcacc tcgggatcgt
     6721 ccgacgttcc tccagctcgt tcatcttcta gcagctgaag cttcaccaga attccgagat
     6781 ttatcatttg tcctaaccga taatcaaatg atccttgacg attcagaagc actggatctt
     6841 gatgatattg atgatactga tatgaatgat caggttgtcg aggtggcacc ggatgttgag
     6901 aacgtcgagg ttcagagtga ttcggaacgt cggaatacgg attcaatacc gttgaaacag
     6961 tttaagacga tccctccgat caatgcgacg acgagtcatt cgacaatatc gattgatgag
     7021 acaccgatga aagcgaagca gcgagaagga tcgctggatg aggagtacgc attgatgaat
     7081 catagtggag gtccgagtga tgcggaagtt cggacgtatg ctggtgatgg agattatgtg
     7141 gagagagatg ttcgagagaa tgatgtgcca acgcgacgaa atactggtgc atcaacatca
     7201 agttacacag gtggtggtcc atattgccta acaaatcgtg gtggttcaaa tgaacgagga
     7261 gccggtttcg gtgaagcagt acgattaact gatggtgttg gaagtggaca tttaaatgat
     7321 gatgattatg ttgaaaaaga gatatcatcc atggatacgc gccggagcac gggcgcctcg
     7381 agctcttcct acggtgttcc acagacgaat tggagtggaa atcgtggtgc cacgtattat
     7441 acgagtaaag ctcaacaggc agcaactgca gcagcagcag cagcagcagc tctccaacag
     7501 caacaaaatg gtggtcgagg cgatcgatta actcaactac ccggaactgg acatttacaa
     7561 tcgacacgtg gtggacaaga tggagattat attgaaactg aaccgaaaaa ttatagaaat
     7621 aatggatctc catcggtact ggattttttt ttttggagaa attagccgga aaattcgatt
     7681 tttttcgctg aaaatctgaa aaaatttggc taaaaattgc cctgaaacac gtttttttcg
     7741 gaattttcat ataaaaatgg acattttcgt aaaaaattgg ctatgtgttg tgaatttttt
     7801 ctgactttta aaggcctttg aactaggaat attaaaattt tattgaaaat tattgaaaag
     7861 tgcaaatttt tatttttttt aggtgaaaaa agcttagatt taggcgaatt ttctgaaatt
     7921 ttatagtgaa tttggttctc aggatattag ccattttttc ccgatttttt ggtaaaaatt
     7981 tgttaaaacc cgcttcaaat gactgtagtt ttatacaaaa ataactaaaa attcgatttt
     8041 tcaattattc agctcgaaaa agcccaacaa aaatatttaa aatttgaaga aaaaagctaa
     8101 aaatgagtaa aaatgactgg tttttttcgc tttttctatt tgaataaatt tctgaaaaat
     8161 gacgaaaatc tgaaaaaatt accccggaca ttgggaaatt tcgaaaaaat cttgaaaata
     8221 gtctgaaatt tttaaaattt acagattttc acaaaaaagg gaaatttcgc atttcctgaa
     8281 attgcccaaa aatttttttt ttcatttttt taaaaattct ggaaatttat ttttggctgt
     8341 ttcaaatttt taaaaaaaga aaaatcccaa aaattggtga aaactccatt aaaattctcc
     8401 agaaaattca aattttccat gaaaaattgt tttaaaagca tgaaaatctg ccgatttttg
     8461 tctaaaattt ttaaaaatat ttcaaaaatt tcagaaaaaa caggaaaagc gaatattaca
     8521 taaataaaaa atctggaaat tctagaaact gaaaattttt taatttttcc gctcaaaaat
     8581 tctaatattc gatgaaaatg agcccgaaaa ttcgagtaaa tttccaaaaa aaattttttt
     8641 ttttttcaaa cacaaatatt aatttttatt aatttttcag cgaaacggca acagccgtga
     8701 cattttcaac ggacgttcgg ctttcggtga aaatgagcat ctaatcgagg ataatgagca
     8761 tccacttgtg gtaccatggt attgatatct gagctccgca tcggccgctg tcatcagatc
     8821 gccatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg
     8881 ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaaac ttcttcttaa
     8941 tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa
     9001 aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa
     9061 taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttttacttc
     9121 tgataaattt tttttgaaac atcatagaaa aaaccgcaca caaaatacct tatcatatgt
     9181 tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct ctcatgacgt
     9241 caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca atcaagtgaa
     9301 agtttatgaa attaattttc ctgcttttgc tttttggggg tttcccctat tgtttgtcaa
     9361 gagtttcgag gacggcgttt ttcttgctaa aatcacaagt attgatgagc acgatgcaag
     9421 aaagatcgga agaaggtttg ggtttgaggc tcagtggaag gtgagtagaa gttgataatt
     9481 tgaaagtgga gtagtgtcta tggggttttt gccttaaatg acagaataca ttcccaatat
     9541 accaaacata actgtttcct actagtggcc acgtgggccg tgcaccttaa gcttggcact
     9601 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     9661 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     9721 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     9781 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     9841 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     9901 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     9961 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
    10021 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
    10081 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
    10141 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
    10201 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
    10261 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
    10321 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
    10381 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
    10441 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
    10501 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
    10561 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    10621 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    10681 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
    10741 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
    10801 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
    10861 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
    10921 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    10981 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
    11041 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    11101 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    11161 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
    11221 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    11281 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    11341 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    11401 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    11461 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    11521 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    11581 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    11641 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    11701 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    11761 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    11821 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    11881 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    11941 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    12001 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    12061 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    12121 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    12181 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    12241 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    12301 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    12361 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    12421 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
//
